Cryopreservation of Spix's yellow-toothed cavy epididymal sperm using Tris- and coconut water-based extenders supplemented with egg yolk or Aloe vera

SummaryThe aim of this study was to establish a functional freezing–thawing protocol for epididymal sperm of collared peccaries (Pecari tajacu L., 1758) by comparing different extenders. The epididymal sperm from 12 sexually mature males was recovered by retrograde flushing using Tris-based or coconut water-based (ACP®-116c) extenders. After initial evaluation, samples were diluted and frozen with the same extenders to which 20% egg yolk and 6% glycerol were added. After 2 weeks, thawing was performed at 37°C/60 s and sperm motility, vigour, morphology, functional membrane integrity, sperm viability, sperm plasma membrane integrity, and a computer-assisted semen analysis (CASA) were assessed. In addition, to evaluate the survival of frozen–thawed sperm, a thermal resistance test (TRT) was executed. Samples preserved using Tris were in better condition compared with those preserved using ACP®, showing higher values for most assessments performed, including CASA and the TRT (P<0.05). After determining Tris to be the better of the two extenders, additional samples were thawed using different thawing rates (37°C/60 s, 55°C/7 s, 70°C/8 s). Sperm thawed at 37°C/60 s had the greatest preservation (P<0.05) of viability (54.1 ± 5.9%) and functional membrane integrity (43.2 ± 5.4%), and had higher values for various CASA parameters. In conclusion, we suggest the use of a Tris-based extender added to egg yolk and glycerol for the cryopreservation of epididymal sperm obtained from collared peccaries. In order to achieve better post-thawing sperm quality, we suggest that samples should be thawed at 37°C/60 s.

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Recovery of epididymal sperm from domestic cats (Felis Catus) using Tris- or powdered coconut water-based solutions

Acta Veterinaria Brasilica ◽

10.21708/avb.2013.7.2.3526 ◽

2013 ◽

Vol 7 (2) ◽

Keyword(s):

Felis Catus ◽

Coconut Water ◽

Domestic Cats ◽

Epididymal Sperm ◽

Water Based

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Comparison between egg yolk-based and coconut water-based extenders to freeze buffalo semen

Reproduction Abstracts ◽

10.1530/repabs.1.p263 ◽

2014 ◽

Author(s):

Mariana Zorzetto ◽

Yame Sancler-Silva ◽

Camila Freitas Dell'aqua ◽

Carlos Ramires Neto ◽

Saulo Zocca ◽

...

Keyword(s):

Egg Yolk ◽

Coconut Water ◽

Water Based ◽

Buffalo Semen

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Kidding rate of artificial insemination with Boer goat liquid semen during chilled preservation using coconut water-based diluent

Jurnal Ilmu-Ilmu Peternakan ◽

10.21776/ub.jiip.2020.030.03.02 ◽

2020 ◽

Vol 30 (3) ◽

pp. 184-189

Author(s):

Muhammad Ade Salim ◽

Muhammad Nur Ihsan ◽

Nurul Isnaini ◽

Trinil Susilawati

Keyword(s):

Egg Yolk ◽

Return Rate ◽

Coconut Water ◽

Chi Square ◽

Boer Goat ◽

Estrous Synchronization ◽

Water Based ◽

Materials Used ◽

Chi Square Analysis

The quality of liquid semen with coconut water as diluent could be preserved up to 3 to 4 days during cold storage at 4 - 5oC. In this research, the successful insemination rate of coconut water-based diluent Boer goat liquid semen was observed to understand the application of coconut water as semen diluent. The materials used in this research were 5 to 7 months Viridis coconut water, liquid semen from 3 years old Boer buck, 30 does for acceptor, and Tris-aminomethane added with 10% egg yolk (EY) as a control diluent. The Boer semen was collected once a week by using artificial vagina, diluted and then stored before used for insemination. The insemination was done by using intra-cervical method at position 3, started with estrous synchronization by using 2 mL PGF2α. The research was conducted as experimental method under randomized group design with two treatments, that were Tris-aminomethane + 10% EY (P0) and coconut water + 10% EY (P1). All of the treatments were repeated for 15 times and the collected data were analyzed with Pearson’s chi square with Genstat 18 program. The observed variables include non-return rate and kidding rate. The results showed that the first non-return rate of P1 were 93.33% and in P0 were 73.33%, while the second non-return rate of P1 were 93.33% and in P0 were 80%. Furthermore, the kidding rate of P1 were 40% and P0 were 66.66%. The Pearson’s chi square analysis showed that there was no effect of coconut water as semen diluent to the kidding rate. The research concludes that the usage of coconut water as semen diluent did not affect the successful insemination rate of Boer goat.

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Kualitas Semen Cair Kambing Boer Berbahan Pengencer Air Kelapa Muda Varietas Viridis Setelah Simpan Dingin

Jurnal Ilmu dan Teknologi Peternakan Tropis ◽

10.33772/jitro.v6i1.5449 ◽

2019 ◽

Vol 6 (1) ◽

pp. 78

Author(s):

Muhammad Ade Salim ◽

Muhammad Nur Ihsan ◽

Nur Isnaini ◽

Trinil Susilawati

Keyword(s):

Semen Quality ◽

Block Design ◽

Animal Husbandry ◽

Egg Yolk ◽

Coconut Water ◽

Boer Goat ◽

Randomized Block Design ◽

Frozen Semen ◽

Artificial Vagina

ABSTRAKAir kelapa muda varietas viridisdapat dijadikan pengencer aletrnatif semen cair bagi program IB di daerah minim sarana semen beku. Tujuan penelitian ini untuk menguji pengaruh penggunaan air kelapa muda viridissebagai bahan pengencer terhadap kualitas semen cair kambing Boer setelah didinginkan. Dilaksanakanselama 3 bulan di Laboratorium Fakultas Peternakan UBUnit SumberSekar,Malang. Metodenya yaitu eksperimen. Semen dari 3 pejantan Boer umur 3-5 tahun, dikoleksi seminggu sekali dengan VB. Air kelapa mudaviridis umur 5-7 bulan serta tris aminomethane sebagai kontrol. Didesain menggunakan Rancangan Acak Kelompok (RAK) dengan 2 perlakuan yaitu P0 (tris aminomethane + 10% KT) dan P1 (air kelapa muda viridis + 10% KT) masing-masing diulang 10 kali. Data dianalisis dengan analisis Ragam (Anova) dengan software Genstat 18. Variabelnya yaitu motilitas individu, viabilitas dan abnormalitas. Hasil penelitian yaitu motilitas individu pada P1bertahan sampai 4 hari (40,5± 24,3%), viabilitas terbaik sampai hari ke-5 (42±24,6%), abnormalitas terendah di hari ke-7(1,31± 0,6). Kesimpulannya, Pengencer air kelapa muda viridis dapat mempertahankan kualitas semen cair kambing Boer selama 4 hari untuk motilitas dan 5 hari untuk viabilitas.Kata Kunci:pengencer, air kelapa, varietas viridisABSTRACTYoung viridis coconut water could be used as an alternative to liquid semen diluent for artificial insemination program in the area with limited facility for frozen semen production. This study evaluated the use of young coconut water as a diluent on liquid semen quality of Boer goat after cold storage. This study was carried out for 3 months at Sumber Sekar Laboratory, Faculty of Animal Husbandry, University of Brawijaya, Malang. The semen was collected from 3 Boer bucks aged at 3 to 5 years old. The semen collection was done once a week with the aid of artificial vagina. The diluents used were young Viridis coconut (5 to 7 months old) and tris aminomethane. The method used was an experiment in a randomized block design with 2 treatments and 10 replicates. The treatments used were T0: tris aminomethane + 10% egg yolk (control) and T1: young Viridis coconut water + 10% egg yolk. Data were analyzed by analysis of variance using Genstat 18 software. The variables measured were sperm individual motility, viability, and abnormality. The results showed that the sperm individual motility in T1 survived up to 4 days (40.5± 24.3%), the best viability at 5 days (42.0±24.6%), while the lowest abnormality at 7 days (1.31±0.6). It could be concluded that: 1. Tris aminomethane diluent has higher quality with the storage length up to 9 days, 2. Young Viridis coconut water diluent could preserve liquid semen quality of Boer goat up to 4 days for sperm motility and 5 days for sperm viability.Keywords: diluents, coconut water, viridis variety

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Determination of Value-Added and Contributing Organization in the Development of Coconut Water-Based Agro Industry

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/709/1/012062 ◽

2021 ◽

Vol 709 (1) ◽

pp. 012062

Author(s):

Hermiza Mardesci ◽

Santosa ◽

Novizar Nazir ◽

Rika Ampuh Hadiguna

Keyword(s):

Value Added ◽

Coconut Water ◽

Water Based

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Development of coconut water-based exopolysaccharide rich functional beverage by fermentation with probiotic Lactobacillus plantarum SVP2

Biocatalysis and Agricultural Biotechnology ◽

10.1016/j.bcab.2021.102030 ◽

2021 ◽

pp. 102030

Author(s):

Louella Concepta Goveas ◽

K.S. Ashwath ◽

Brejeth Renita Nazerath ◽

Ostrin Dsouza ◽

Ullekh ◽

...

Keyword(s):

Lactobacillus Plantarum ◽

Coconut Water ◽

Probiotic Lactobacillus ◽

Water Based

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Network Meta-Analysis of 10 Storage Mediums for Preserving Avulsed Teeth

Frontiers in Medicine ◽

10.3389/fmed.2021.749278 ◽

2021 ◽

Vol 8 ◽

Author(s):

Na Zhang ◽

Yuzhao Cheng ◽

Fenglan Li ◽

Qian Kang

Keyword(s):

Green Tea ◽

Meta Analysis ◽

Aloe Vera ◽

Egg White ◽

Coconut Water ◽

Cochrane Library ◽

Oral Rehydration ◽

Pdl Cells ◽

Comprehensive Search ◽

The Cochrane Library

Many storage mediums are available for the storage of avulsed teeth to preserve the viability of periodontal ligament (PDL) cells before replantation; however, it is unclear which medium is the optimal option. We performed this network meta-analysis to answer this question. A comprehensive search was conducted in PubMed, EMBASE, and the Cochrane library to capture eligible studies investigating the comparative efficacy of Hank's balanced salt solution (HBSS), aloe vera gel (AVG), oral rehydration solution (ORS), coconut water, egg white, green tea, propolis, saline, milk, and water. Statistical analysis was conducted using Review Manager v5.3 and ADDIS v1.16.8. In total, 20 RCTs involving 31 reports were included finally. Direct meta-analysis suggested that HBSS was superior to ORS, milk, saline, and water, ORS was superior to milk but inferior to coconut water and propolis, egg white was superior to milk but inferior to AVG and propolis, propolis was superior to AVG, milk, and saline, and coconut water and water was inferior to saline and milk, respectively. Network meta-analysis suggested that AVG was inferior to the other nine mediums, and propolis was superior to HBSS (SMD, −5260.24; 95% CrI, −10447.39 to −70.37) and milk (SMD, −5461.11; 95% CrI, −10574.99 to −328.51). Moreover, ranking probabilities indicated the highest probability for propolis, followed by saline, ORS, HBSS, milk, egg white, water, green tea, and AVG successively. Propolis may be the optimal media for storing avulsed teeth before replantation. However, given the availability of propolis and HBSS and the hypotonic properties of saline, ORS or milk should also be preferentially selected.

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