Gene Expression in Embryos From Norwegian Red Bulls With High or Low Non Return Rate: An RNA-Seq Study of in vivo-Produced Single Embryos

During the last decade, paternal effects on embryo development have been found to have greater importance than previously believed. In domestic cattle, embryo mortality is an issue of concern, causing huge economical losses for the dairy cattle industry. In attempts to reveal the paternal influence on embryo death, recent approaches have used transcriptome profiling of the embryo to find genes and pathways affected by different phenotypes in the bull. For practical and economic reasons, most such studies have used in vitro produced embryos. The aim of the present study was to investigate the differences in the global transcriptome of in vivo produced embryos, derived from sires with either high or low field fertility measured as the non-return rate (NRR) on day 56 after first AI of the inseminated cows. Superovulated heifers (n = 14) in the age span of 12–15 months were artificially inseminated with semen from either high fertility (n = 6) or low fertility (n = 6) bulls. On day seven after insemination, embryos were retrieved through uterine flushing. Embryos with first grade quality and IETS stage 5 (early blastocyst), 6 (blastocyst) or 7 (expanded blastocyst) were selected for further processing. In total, RNA extracted from 24 embryos was sequenced using Illumina sequencing, followed by differential expression analysis and gene set enrichment analysis. We found 62 genes differentially expressed between the two groups (adj.p-value<0.05), of which several genes and their linked pathways could explain the different developmental capacity. Transcripts highly expressed in the embryos from low fertility bulls were related to sterol metabolism and terpenoid backbone synthesis, while transcripts highly expressed in the high fertility embryos were linked to anti-apoptosis and the regulation of cytokine signaling. The leukocyte transendothelial migration and insulin signaling pathways were associated with enrichments in both groups. We also found some highly expressed transcripts in both groups which can be considered as new candidates in the regulation of embryo development. The present study is an important step in defining the paternal influence in embryonic development. Our results suggest that the sire’s genetic contribution affects several important processes linked to pre-and peri implantation regulation in the developing embryo.

The Effect of Leisure Involvement and Leisure Satisfaction on the Well-Being of Pickleball Players

The purpose of this study is to investigate the effect of leisure involvement and leisure satisfaction on the well-being of pickleball players. This study enrolled 260 participants from the 2019 International Pickleball Tournament by purposive sampling. A total of 250 questionnaires were returned, for a return rate of 96%; 215 questionnaires were valid, for an effective recovery rate of 86%. The data were archived using SPSS 24.0, and the correlation between variables was analyzed using AMOS 24.0. By analyzing the empirical data in this paper, the following main findings were obtained: (1) leisure involvement has a significant effect on leisure satisfaction; (2) leisure involvement does not have a significant effect on well-being; (3) leisure satisfaction has a significant effect on well-being; and (4) leisure satisfaction has a mediating effect on the relationship between leisure involvement and well-being.

Semen Viability Properties of Sexually Stimulated Boars on the Fertilization Capacity of Artificially Inseminated Large White Sows

Background: This study aimed to investigate the influences of sexual preparation on the effects of boars’ semen viability on the fertilization capacity of artificially inseminated sows. After all, boar sires more pigs than farrowed by a sow which the boar has been mated to improve reproductive parameters in response to AI. Methods: The semen viability of boars were studied during various sexual stimulations and analyzed during the study period. Sperm rich fractions were collected and separated at every level of sexual stimulation during the morning (08:30) and the afternoon (14:30) hours, respectively. Artificial insemination was performed following three levels of sexual preparations of boars (0 minutes of sexual restraint (MSR), 5 MSR and 10 MSR) before semen collection. Receptive sows were inseminated and evaluated for fertility traits using non-return rate, farrowing rate and litter size. Result: The non-return rate was recorded as a percentage of sows conceived after insemination over the total number of sows inseminated. The farrowing rate was recorded as a percentage of sows that farrows over the number of sows conceived and litter size as several live piglets per sow. Sexual desire was influenced by sexual preparations and significantly influenced the fertility of the artificially inseminated sows. This study is of practical significance to the animal breeder mainly because boars have greater influence than sows on the average litter size and live piglets. The study concludes that the use of at least 5 to 10 minutes of sexual restraint during the afternoon periods prior to semen collection and artificial insemination is found to be a practical method for optimizing sperm viability and fertility of sows in the intensive system.

Adjustable Security Proportions in the Fuzzy Portfolio Selection under Guaranteed Return Rates

Based on the concept of high returns as the preference to low returns, this study discusses the adjustable security proportion for excess investment and shortage investment based on the selected guaranteed return rates in a fuzzy environment, in which the return rates for selected securities are characterized by fuzzy variables. We suppose some securities are for excess investment because their return rates are higher than the guaranteed return rates, and the other securities whose return rates are lower than the guaranteed return rates are considered for shortage investment. Then, we solve the proposed expected fuzzy returns by the concept of possibility theory, where fuzzy returns are quantified by possibilistic mean and risks are measured by possibilistic variance, and then we use linear programming model to maximize the expected value of a portfolio’s return under investment risk constraints. Finally, we illustrate two numerical examples to show that the expected return rate under a lower guaranteed return rate is better than a higher guaranteed return rates in different levels of investment risks. In shortage investments, the investment proportion for the selected securities are almost zero under higher investment risks, whereas the portfolio is constructed from those securities in excess investments.

Return Sponsorships in the EU’s New Pact on Migration and Asylum: High Stakes, Low Gains

The concept of ‘return sponsorships’ is central to the European Commission’s New Pact on Migration and Asylum, and its ambition to deliver a “fresh start on migration”. Enabling a system of mandatory yet flexible solidarity, the concept is designed to overcome the opposing viewpoints that have long marked political discussions over responsibility-sharing within Europe. This article critically considers whether return sponsorships can work in practice. It identifies three challenges. First, the proposal’s envisioned solidarity measures lack predictability and tangibility. Second, return sponsorships will create new vulnerabilities for those issued return orders. Third, the mechanism is informed by the improbable assumption that more conditionalities vis-à-vis third countries will substantially increase the EU’s return rate. Overall, the Commission is taking a considerable risk. The return sponsorship concept will likely prolong the polarised discussions among Member States and provide only modest support to countries at the EU’s external border.

902. Unreturned Pill Bottles in the 1489 and 1490 Clinical Trials: An Important Measure of Poor Adherence that Is Often Ignored in Pill Count Calculations

Background Adherence to antiretroviral therapy is important for HIV suppression. In clinical trials, adherence is commonly measured by pill count; limitations are that unreturned pills may not have been taken and unreturned pill bottle data are omitted. This analysis focuses on the relationship between unreturned pill bottles as a measure of poor adherence and the overall effect on virologic success rates across all treatment groups. Methods Pill bottle return category (all bottles returned or ≥1 bottle unreturned) and return rates for participants across all treatment groups from two treatment-naïve INSTI clinical trials (Studies 1489 and 1490) were calculated. Association of bottle return category or rates with virologic events through week 144, including last on-treatment observation carried forward (LOCF) outcome, was determined; comparisons used Fisher’s exact or Wilcoxon rank sum test. Results Virologic suppression with ≥95% adherence by pill count can differ for those with unreturned pill bottles (Figure). In these studies, 60% of participants returned all their pill bottles through week 144; if one visit with ≥1 unreturned bottle was allowed, this percentage increased to 81%. The mean bottle return rate was 94% and did not differ by study, treatment arm, or sex. Failure to return pill bottles was significantly associated with lower suppression rates. Additionally, significant differences in pill bottle return rate (p < 0.01) were observed by week 144 LOCF outcome (95% vs 77% mean return rate for those with HIV RNA < 50 c/mL vs ≥50 c/mL), need for resistance testing (95% vs 77% return rate for those not tested vs tested), confirmed virologic failure (VF) (94% vs 90% return rate for those without VF vs with VF) and blip status (95% vs 92% return rate for those without blips vs with blips). HIV-1 Viral Loads of Two Participants with ≥95% Adherence by Pill Count through Week 144 Conclusion In these treatment-naïve INSTI clinical trials, failure to return pill bottles was associated with lower suppression rates. Although the calculated adherence rates in these studies was relatively high (median ≥95%), these calculations did not account for unreturned pill bottles. We believe that assessing adherence by both pill count and pill bottle return rate may provide a more complete picture of adherence in clinical trials. Disclosures Rima K. Acosta, BS, Gilead Sciences, Inc. (Employee, Shareholder) Grace Q. Chen, BS, Gilead Sciences, Inc. (Employee) Hailin Huang, PhD, Gilead Sciences, Inc. (Employee, Shareholder) Hui Liu, PhD, Gilead Sciences, Inc. (Employee, Shareholder) Kirsten L. White, PhD, Gilead Sciences, Inc (Employee, Shareholder)

Sustainable Design of a Multi-Echelon Closed Loop Supply Chain under Uncertainty for Durable Products

The increased awareness of environmental sustainability has led to increasing attention to closed loop supply chains (CLSC). The main objective of the CLSC is to capture values from end-of-life (EOL) products in a way that ensures a business to be economically and environmentally sustainable. The challenge is the complexity that occurrs due to closing the loop. At the same time, considering stochastic variables will increase the realism of the obtained results as well as the complexity of the model. This study aims to design a CLSC for durable products using a multistage stochastic model in mixed-integer linear programming (MILP) while considering uncertainty in demand, return rate, and return quality. Demand was described by a normal distribution whereas return rate and return quality were represented by a set of discrete possible outcomes with a specific probability. The objective function was to maximize the profit in a multi-period and multi-echelon CLSC. The multistage stochastic model was tested on a real case study at an air-conditioning company. The computational results identified which facilities should be opened in the reversed loop to optimize profit. The results showed that the CLSC resulted in a reduction in purchasing costs by 52%, an annual savings of 831,150 USD, and extra annual revenue of 5459 USD from selling raw material at a material market. However, the transportation cost increased by an additional annual cost of 6457 USD, and the various recovery processes costs were annually about 152,897 USD. By running the model for nine years, the breakeven point will be after three years of establishing the CLSC and after the annual profit increases by 1.92%. In conclusion, the results of this research provide valuable analysis that may support decision-makers in supply chain planning regarding the feasibility of converting the forward chain to closed loop supply chain for durable products.

Improving the completion rate of outpatient stool-based colon cancer screening during a global pandemic.

238 Background: Colon Cancer Screening is an important intervention to reduce the morbidity and mortality of colorectal cancer. In particular, stool based screening tests help to increase the availability of screening especially amidst a global pandemic. However stool based screening is limited by patient return rates of FIT/iFOBT tests. Here at the Austin Outpatient VA we sought to improve the FIT test return rate of patients in our resident clinic by at least 20%. Methods: We did a root cause analysis as to why iFOBT/FIT were not being returned, particularly during the height of the pandemic when most visits were virtual. With help from the VA data core we generated a list of 76 patients during a period from 2/3/2020-7/7/2020 who had not returned an ordered FIT/iFOBT test in >30 days. We called these patients to identify the reasons why they did not return their test. Results: We were able to reach (44/76) patients and discovered that only (12/44) 27.3% of patients contacted had received a FIT test. Of our patients-reached 33/44 of them asked for a new FIT test to be sent to them and ultimately (21/44) patients contacted returned and completed their FIT test successfully with 3 tests being positive and referred to diagnostic colonoscopy. This resulted in an improvement in return rate of ̃47% of all patients ultimately contacted. Conclusions: Through a meeting of stakeholders including nurses and resident physicians we discovered in our EMR, that the order to send out a FIT test does not automatically alert a nurse nor does it automatically mail out or send out a FIT test. We discovered there was not a standardized process in place to facilitate communication between physicians ordering tests and nurses, thus FIT/FOBT tests were not being sent. We are currently working on a process to standardize the FIT test ordering process to improve communication between physicians and nurses. We are developing a better order set that reminds providers to alert and message nurses prior to ordering the FIT/iFOBT test. We are also educating Residents about the FIT/FOBT ordering process. With future PDSA cycles we should be able to improve the robustness and reliability of our outpatient colon cancer screening process through improved inter-professional communication.