Genetic diversity and species differentiation of medicinal plant Persian Poppy (Papaver bracteatum L.) using AFLP and ISSR markers

2020 ◽  
Vol 16 ◽  
pp. 100058
Author(s):  
Mahdi Hadipour ◽  
S. Kamal Kazemitabar ◽  
Hamid Yaghini ◽  
Soleyman Dayani
Keyword(s):  
Genetic Diversity ◽  
Medicinal Plant ◽  
Issr Markers ◽  
Species Differentiation ◽  
Papaver Bracteatum

ISSR Marker Based Genetic Diversity in Morinda Spp. For Its Enhanced Collection, Conservation and Utilization

2021 ◽  
Author(s):  
Lalit Arya ◽  
Ramya Kossery Narayanan ◽  
Anjali Kak ◽  
Chitra Devi Pandey ◽  
Manjusha Verma ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Gene Diversity ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Morinda Citrifolia ◽  
Species Differentiation ◽  
Information Index ◽  
Upgma Cluster Analysis ◽  
Simple Sequence

Abstract Morinda (Rubiaceae) is considerably recognized for its multiple uses viz. food, medicine, dyes, firewood, tools, oil, bio-sorbent etc. The molecular characterization of such an important plant would be very useful for its multifarious enhanced utilization. In the present study, 31 Morinda genotypes belonging to two different species Morinda citrifolia and Morinda tomentosa collected from different regions of India were investigated using Inter Simple Sequence Repeat (ISSR) markers. Fifteen ISSR primers generated 176 bands with an average of 11.7 bands per primer, of which (90.34%) were polymorphic. The percentage of polymorphic bands, mean Nei’s gene diversity, mean Shannon’s information index in Morinda tomentosa and Morinda citrifolia was [(69.89%, 30.68%); (0.21 ± 0.19, 0.12 ± 0.20); (0.32 ± 0.27 0.17 ± 0.28)] respectively, revealing higher polymorphism and genetic diversity in Morinda tomentosa compared to Morinda citrifolia. Structure, and UPGMA cluster analysis placed the genotypes into well-defined separate clusters belonging to two species Morinda tomentosa and Morinda citrifolia revealing the utility of ISSR markers in species differentiation. Distinct ecotypes within a particular species could also be inferred emphasizing the collection and conservation of Morinda genotypes from different regions, in order to capture the overall diversity of respective species. Further higher diversity of M. tomentosa must be advanced for its utilization in nutraceutical, nutritional and other nonfood purposes.

scholarly journals Assessment of genetic diversity among India Ginseng, Withania somnifera (L) Dunal using RAPD and ISSR markers

2016 ◽  
Vol 7 ◽  
Author(s):  
Shahana Khan ◽  
Rehana Anjum Shah
Keyword(s):  
Genetic Diversity ◽  
Medicinal Plant ◽  
Withania Somnifera ◽  
Population Analysis ◽  
Issr Markers ◽  
Neighbor Joining ◽  
Indian Ginseng ◽  
Medicinal Use ◽  
Wide Range ◽  
Rapd And Issr

<em>Withania somnifera</em> is commonly known as Indian Ginseng or Ashwagandha which is listed highly medicinal plant in Ayurveda for its wide range of medicinal use. Genetic diversity among 16 genotypes of <em>Withania somnifera</em> collected from four different regions viz. Lucknow, Nimuch, Karnataka and Mumbai was studied using RAPD and ISSR techniques. The ISSR and RAPD together produced 89 bands across 16 genotypes of <em>W. somnifera </em>of which 39 were polymorphic showing 47.89% polymorphism. Over 99% of the RAPD and ISSR fragments were reproducible in the present experiment. There was 90% uniformity within the population. The neighbor joining tree also confirmed a clear grouping and differentiation based on populations of origin i.e. samples were grouped on the basis of their regions of origin. The dendrogram reveals that outgroup of <em>W. somnifera </em>Karnataka origin are evolutionarily related to <em>W. somnifera </em>Nimuch group varieties and <em>W. somnifera </em>Lucknow and Mumbai group varieties are evolutionarily closely related. Within population analysis of Lucknow and Nimuch location showed two sub groups of two lines each showing slight diversity. Plants from Karnataka and Mumbai region show less diversity within population.

scholarly journals KERAGAMAN GENETIK ALANG-ALANG (Imperata cylindrica (L.) Beauv.) BERDASARKAN MARKA INTER-SIMPLE SEQUENCE REPEATS (ISSR)

2018 ◽  
Vol 17 (2) ◽  
Author(s):  
Dyah Subositi ◽  
Harto Widodo
Keyword(s):  
Genetic Diversity ◽  
Medicinal Plant ◽  
Ethnic Groups ◽  
Simple Sequence Repeats ◽  
Issr Markers ◽  
Imperata Cylindrica ◽  
Sample Collection ◽  
Issr Primer ◽  
Inter Simple Sequence Repeats ◽  
Simple Sequence

Alang-alang (Imperata cylindrica (L.) Beauv.) has been widely used as a medicinal plant to treat some diseases, such as fever, headache, and diuretic. Nowadays, there is no information of genetic diversity of this plant used in herbal formula by ethnic groups in Indonesia. The main objective of this study was to asses genetic diversity of alang-alang from 18 selected ethnic groups in Indonesia based on Inter Simple Sequence Repeats (ISSR). Location of sample collection was identified by using data on Research on Medicinal Plant (Ristoja) 2012. Total DNA genome was isolated and ISSR primer screening were done on collected samples. Ten selected ISSR primers produced 74 amplified DNA fragments 58, fragments (78.4 %) were polymorphic. Dice index similarity was used to construct UPGMA dendrogram. The genetic similarity indexing which among accessions was ranged from 70.5–90.5% thereby indicating a low level of genetic diversity occurred in alang-alang. The results of this study also showed that ISSR markers were able to genetically differentiate alang-alang accessions by which this information can be useful for further researchs such as for standardization of medicinal plants. 

Assessment of genetic diversity in Solanum trilobatum L., an important medicinal plant from South India using RAPD and ISSR markers

2013 ◽  
Vol 60 (3) ◽  
pp. 807-818 ◽  
Author(s):  
Jayabalan Shilpha ◽  
Tamilarasan Silambarasan ◽  
Shunmugiah Karutha Pandian ◽  
Manikandan Ramesh
Keyword(s):  
Genetic Diversity ◽  
Medicinal Plant ◽  
South India ◽  
Issr Markers ◽  
Solanum Trilobatum ◽  
Rapd And Issr ◽  
Important Medicinal Plant

Genetic Diversity Among Saudi Peganum harmala and Rhazya stricta Populations Using Chemical and ISSR Markers

2019 ◽  
Vol 20 (13) ◽  
pp. 1134-1146
Author(s):  
Magda E. Abd-Elgawad ◽  
Modhi O. Alotaibi
Keyword(s):  
Genetic Diversity ◽  
Folk Medicine ◽  
Issr Markers ◽  
Wild Plants ◽  
Gas Chromatography Mass Spectrometry ◽  
Group Method ◽  
Peganum Harmala ◽  
Molecular Polymorphism ◽  
Arithmetic Average ◽  
Rhazya Stricta

Background:The vernacular name 'Harmal' is used for two plant species in Saudi Arabia, i.e. Peganum harmala L. and Rhazya stricta Decne. Both are important medicinal plants which offer interesting pharmacological properties.Objective:This study aimed to evaluate the genetic diversity among different populations of harmal based on chemical variations of alkaloids and molecular polymorphism.Methods:Total alkaloids were extracted from plants of three populations of each species and estimated by using spectrophotometer and the chemical compounds were analyzed by Gas chromatography mass spectrometry (GC-MS). Molecular polymorphism was estimated by using the Inter Simple Sequence Repeat (ISSR) fingerprints.Results:The results showed that the alkaloids content of R. stricta was higher than P. harmala populations. The GC-MS analysis revealed the presence of (65-53) compounds in R. stricta and P. harmala, and the percentage of polymorphism was found to be 93.2%. Sixteen ISSR primers produced 170 scorable bands with an average of 9.6 bands per primer and 75%-100% polymorphism. The cluster analysis using the unweighted pair-group method of the arithmetic average (UPGMA) method based on combined data of GC-MS and ISSR markers divided the six harmal genotypes into two major groups.Conclusion:The existence of variations in chemical and genetic markers is useful for the selection of potential genotypes for medicinal use, and for breeding lines for medicinal substances production to spare wild plants from uncontrolled harvesting for folk medicine.

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