scholarly journals Assessment of genetic diversity among India Ginseng, Withania somnifera (L) Dunal using RAPD and ISSR markers

2016 ◽  
Vol 7 ◽  
Author(s):  
Shahana Khan ◽  
Rehana Anjum Shah
Keyword(s):  
Genetic Diversity ◽  
Medicinal Plant ◽  
Withania Somnifera ◽  
Population Analysis ◽  
Issr Markers ◽  
Neighbor Joining ◽  
Indian Ginseng ◽  
Medicinal Use ◽  
Wide Range ◽  
Rapd And Issr

<em>Withania somnifera</em> is commonly known as Indian Ginseng or Ashwagandha which is listed highly medicinal plant in Ayurveda for its wide range of medicinal use. Genetic diversity among 16 genotypes of <em>Withania somnifera</em> collected from four different regions viz. Lucknow, Nimuch, Karnataka and Mumbai was studied using RAPD and ISSR techniques. The ISSR and RAPD together produced 89 bands across 16 genotypes of <em>W. somnifera </em>of which 39 were polymorphic showing 47.89% polymorphism. Over 99% of the RAPD and ISSR fragments were reproducible in the present experiment. There was 90% uniformity within the population. The neighbor joining tree also confirmed a clear grouping and differentiation based on populations of origin i.e. samples were grouped on the basis of their regions of origin. The dendrogram reveals that outgroup of <em>W. somnifera </em>Karnataka origin are evolutionarily related to <em>W. somnifera </em>Nimuch group varieties and <em>W. somnifera </em>Lucknow and Mumbai group varieties are evolutionarily closely related. Within population analysis of Lucknow and Nimuch location showed two sub groups of two lines each showing slight diversity. Plants from Karnataka and Mumbai region show less diversity within population.

Assessment of genetic diversity in Solanum trilobatum L., an important medicinal plant from South India using RAPD and ISSR markers

2013 ◽  
Vol 60 (3) ◽  
pp. 807-818 ◽  
Author(s):  
Jayabalan Shilpha ◽  
Tamilarasan Silambarasan ◽  
Shunmugiah Karutha Pandian ◽  
Manikandan Ramesh
Keyword(s):  
Genetic Diversity ◽  
Medicinal Plant ◽  
South India ◽  
Issr Markers ◽  
Solanum Trilobatum ◽  
Rapd And Issr ◽  
Important Medicinal Plant

scholarly journals Molecular analysis of genetic diversity in population of Silybum marianum (L.) Gaertn in Egypt

2019 ◽  
Vol 17 (1) ◽  
Author(s):  
Marwa Hamouda
Keyword(s):  
Genetic Diversity ◽  
Electrophoretic Pattern ◽  
Issr Markers ◽  
Silybum Marianum ◽  
Inter Simple Sequence Repeats ◽  
Sds Page ◽  
Dna Pattern ◽  
Pharmaceutical Properties ◽  
High Level ◽  
Rapd And Issr

Abstract Background Silybum marianum L. Gaertn is a medicinal plant of unique pharmaceutical properties in the treatment of liver disorders and diabetic nephropathy. Biochemical (SDS-PAGE) and molecular markers such as randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) technologies were used in this work to detect genetic diversity of 14 collections of Silybum marianum population in Egypt. Results The electrophoretic pattern of seed protein gave different molecular weight bands, ranging from 24 to 111 KDa with the presence of unique bands. RAPD results revealed a high level of polymorphism (73.2%) using 12 RAPD primers, but only eight of them gave reproducible polymorphic DNA pattern. Sixteen primers were used in the ISSR method; only ten of them yielded clearly identifiable bands. The percentage of polymorphism is about 80% of the studied samples. Conclusion The obtained data confirmed that SDS-protein, RAPD, and ISSR markers are important tools for genetic analysis for Silybum marianum and recommended to give accurate results.

scholarly journals Efficiency of improved RAPD and ISSR markers in assessing genetic diversity and relationships in Angelica sinensis (Oliv.) Diels varieties of China

2015 ◽  
Vol 18 (2) ◽  
pp. 96-102 ◽  
Author(s):  
Zhiqiang Mei ◽  
Chun Zhang ◽  
Md. Asaduzzaman Khan ◽  
Ye Zhu ◽  
Mousumi Tania ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Issr Markers ◽  
Angelica Sinensis ◽  
Rapd And Issr

scholarly journals MIRACLE AYURVEDIC HERB - ASHWAGANDHA (WITHANIA SOMNIFERA DUNAL)

2021 ◽  
Vol 9 (8) ◽  
pp. 1778-1788
Author(s):  
Bargale Sushant Sukumar
Keyword(s):  
Withania Somnifera ◽  
Chemical Constituents ◽  
Immunomodulatory Activity ◽  
Indian Ginseng ◽  
Aerial Parts ◽  
Wide Range ◽  
The World ◽  
Anti Inflammatory ◽  
Ayurvedic Herb ◽  
Present World

Ayurveda is successfully implemented as a primary healthcare system in India, and it is flourishing in the promotion of health around the world. Ayurveda aims to create a society happily, healthy, and peaceful. In general, the simple regimes mentioned in Ayurveda texts are of immense use faced by the present world today regarding health pro- motion. Ashwagandha (Withania Somnifera) is usually referred to as 'Indian winter cherry' or 'Indian Ginseng'. It is a shrub grown in India and North America whose roots have been used by Ayurvedic practitioners for thousands of years. Ashwagandha is one of Ayurveda's most essential herbs, used as Rasayana for its wide range of health benefits for centuries. Chemicals constitute of Ashwagandha (Withania Somnifera)- The chemistry of Ashwagan- dha (Withania Somnifera) has been widely studied and numerous categories of chemical constituents have been described, extracted, and isolated, such as steroidal lactones, alkaloids, flavonoids, tannin, etc. More than 12 alka- loids, 40 withanolides and several sitoindosides have currently been isolated from the aerial parts, roots, and berries of the Withania species and have been recorded. The principal biochemical element of Ashwagandha (WS) root is withanolides, which are steroidal alkaloids and steroidal lactones. The pharmacological activity of Ashwagan- dha- It has been shown to have pharmacological importance as an Adaptogen, Antibiotic, abortifacient, aphrodisiac, Astringent, Anti-inflammatory, deobstructive, diuretic, narcotic, sedative, and tonic through centuries of Ayurvedic medicinal practice using Ashwagandha (Withania Somnifera). Along with these Ashwagandha acts as Anti-stress,Anticarcinogenic activity, Anti-inflammatory activity, Anti-aging activity, Cardioprotective activity, hypothyroid activity and Immunomodulatory activity. Keywords: Ashwagandha, Withania Somnifera, Alkaloids, Withanoids, sitoindosides

scholarly journals Assessment of genetic diversity in Colletotrichum falcatum Went accessions based on RAPD and ISSR markers

2018 ◽  
Vol 16 (1) ◽  
pp. 153-159
Author(s):  
Prittesh Patel ◽  
B.K. Rajkumar ◽  
Preeti Parmar ◽  
Rusabh Shah ◽  
R. Krishnamurthy
Keyword(s):  
Genetic Diversity ◽  
Issr Markers ◽  
Colletotrichum Falcatum ◽  
Rapd And Issr
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