Design, synthesis, acetylcholinesterase inhibition and larvicidal activity of girgensohnine analogs on Aedes aegypti, vector of dengue fever

Background and Aim: Aedes aegypti is the vector of dengue fever, dengue hemorrhagic fever, chikungunya, and, most recently, Zika. Dengue fever is one of Indonesia's endemic diseases. The principal tool for preventing dengue is controlling Ae. aegypti by chemical insecticides since vaccine against dengue is still under research. However, Ae. aegypti developed resistance to various chemical insecticides worldwide. Therefore, research on alternate compounds as mosquito insecticides is urgently needed. This study demonstrated the efficacy of Artemisia vulgaris extract as larvicidal, ovicidal, adulticidal, repellency, and oviposition deterrent activity against Ae. aegypti. Materials and Methods: A. vulgaris was obtained from Temanggung, Indonesia, while the eggs of Ae. aegypti were collected from Yogyakarta, Indonesia, and were hatched in Laboratory of Parasitology, Faculty of Veterinary Medicine, Universitas Gadjah Mada. Larvicidal activity was evaluated according to the WHO protocol; adulticidal activity was performed using the Centers for Disease Control protocol. Oviposition activity was evaluated using ovitraps added with A. vulgaris extract, complete protection time in the repellent assay was defined as the number of minutes elapsed between compound application and the landing of the first mosquito. Results: A test of the larvicidal activity of A. vulgaris extract returned an LC50 of 65.8 ppm (r2=0.9014) in 1 h and 18.6 ppm (r2=0.575) in 24 h. A. vulgaris was effective as an adulticidal, demonstrating LC50 values of 11.35 mg (r2=0.875) in 90 min, 9.63 mg (r2=0.924) in 105 min, and 6.46 mg (r2=0.925) in 120 min. A. vulgaris at a concentration of 1000 ppm was able to reach 96% of oviposition deterrent effect. The ovicidal assay, a concentration of 1000 ppm resulted in 82.67% of eggs remaining unhatched. An extract concentration of 80 mg/ml achieved 63.3±3.5% biting repellency in adults. Conclusion: This study gives a clear indication that A. vulgaris extract acts on Ae. aegypti at various developmental stages and is a potential alternative bioinsecticide for controlling this disease vector.

Download Full-text

Larvicidal activity of extracellular secondary metabolites from a Stereum species Hill ex Pers. (JO5289) against the dengue fever mosquito, Aedes aegypti (Linn) (Diptera: Culicidae)

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb2013.12447 ◽

2013 ◽

Vol 12 (44) ◽

pp. 6302-6309 ◽

Cited By ~ 2

Author(s):

Kipngeno Chirchir Dennis ◽

Ben Omondi Ouma Russell ◽

Kiplagat Cheplogoi Peter ◽

Ouma Omolo Josiah

Keyword(s):

Secondary Metabolites ◽

Aedes Aegypti ◽

Dengue Fever ◽

Larvicidal Activity

Download Full-text

Environmental and cultured cyanobacteria as sources of Aedes aegypti larvicides

Universitas Scientiarum ◽

10.11144/javeriana.sc24-3.eacc ◽

2019 ◽

Vol 24 (3) ◽

pp. 465-496

Author(s):

Farja I Ayala ◽

Laura M Becerra ◽

Jairo Quintana ◽

Lina M Bayona ◽

Freddy A Ramos ◽

...

Keyword(s):

Public Health ◽

Aedes Aegypti ◽

Bioactive Compounds ◽

Larvicidal Activity ◽

Artemia Salina ◽

Acetylcholinesterase Inhibition ◽

Effective Control ◽

Benthic Cyanobacteria ◽

Organic Extracts ◽

Chemical Profiles

In tropical countries, the control of the mosquito Aedes aegypti is a public health priority due to its role as a vector of important viral diseases. Marine cyanobacteria are recognized as abundant sources of bioactive compounds, and they constitute a potential source of insecticides useful for controlling mosquito populations and preventing epidemic outbreaks. We collected 30 benthic cyanobacterial mats in Providencia and Rosario islands (in the Colombian Caribbean) belonging to the genera Phormidium, Symploca, Oscillatoria, Lyngbya, Pseudoanabaena, Leptolyngbya, Moorea, and Dapis. Fractions of organic extracts from the most abundant environmental samples were evaluated in three bioassays, assessing (i) larvicidal activity against A. aegypti, (ii) toxicity against the brine shrimp (Artemia salina) nauplii, and (iii) acetylcholinesterase inhibition. Non-polar fractions exhibited larvicidal activity. The polar fraction from one Dapis pleuosa extract showed larvicidal activity without being toxic against A. salina nauplii. Extracts from Moorea producens exhibited the greatest toxicity against A. aegypti larvae and A. salina nauplii. From 23 cultured cyanobacterial samples, only five grew under laboratory conditions and produced enough biomass to yield organic extracts. Of these, three extracts showed strong larvicidal activity, but only the extract from Phormidium tenue showed reduced toxicity against A. salina nauplii. We detected variation among the chemical profiles and larvicidal activity of cyanobacterial consortia depending on sites and dates of collection. Our findings suggest that despite variation in chemical profiles, extracts of marine benthic cyanobacteria can be further developed as effective control agents against insect vectors, in their larval stages. The culture of marine benthic cyanobacteria needs to be further explored to provide enough biomass leading to the identification of bioactive compounds with public health applications.

Download Full-text

Synthesis and characterization of Mn(I) complexes and their larvicidal activity against Aedes aegypti, vector of dengue fever

Inorganic Chemistry Communications ◽

10.1016/j.inoche.2017.07.018 ◽

2017 ◽

Vol 84 ◽

pp. 49-55 ◽

Cited By ~ 4

Author(s):

Inara de Aguiar ◽

Edjane Rocha dos Santos ◽

Ana Carolina Mafud ◽

Vinicius Annies ◽

Mario Antonio Navarro-Silva ◽

...

Keyword(s):

Aedes Aegypti ◽

Dengue Fever ◽

Larvicidal Activity ◽

Synthesis And Characterization

Download Full-text

Larvicidal, adulticidal, and oviposition-deterrent activity of Piper betle L. essential oil to Aedes aegypti

Veterinary World ◽

10.14202/vetworld.2019.367-371 ◽

2019 ◽

Vol 12 (3) ◽

pp. 367-371 ◽

Cited By ~ 3

Author(s):

Riesna Martianasari ◽

Penny Humaidah Hamid

Keyword(s):

Essential Oil ◽

Aedes Aegypti ◽

Dengue Fever ◽

Larvicidal Activity ◽

Mosquito Control ◽

Activity Index ◽

Piper Betle ◽

Primary Vector ◽

Aegypti Population ◽

Deterrent Activity

Background and Aim: Aedes aegypti is a primary vector of many arthropod-borne diseases. One of the diseases, dengue fever, is an endemic disease in Indonesia causing high mortalities for decades. There are no preventive and specific treatments for dengue so far. Therefore, prevention of this disease largely depends on the mosquito control. Since resistance to chemical insecticides occurred worldwide, the study on alternate and new mosquito insecticides are mandatory. This study aimed to demonstrate the effect of essential oil from P. betle L. in the larval and adult stages, as well as its influence on oviposition activity of A. aegypti mosquito. Materials and Methods: P. betle efficacy was evaluated in various stages of A. aegypti development. For the larvicidal activity, larvae instar III stage was used. Adulticidal assay in this experiment was performed using newly emerged A. aegypti. For oviposition assay, mated A. aegypti was tested for their responses to P. betle-treated and non-treated ovitraps. Results: P. betle L. - adulticide activity was effective with a concentration of 2.5 μl/ml, caused 100% mortality within 15-30 min. Larvicide activity was observed after 1 h, 24 h, and 48 h post-treatment with LC50183, 92.7, and 59.8 ppm and LC90> 637, 525, and 434.7 ppm, respectively. Oviposition activity index was −0.917 in 1000 ppm. In addition, the eggs number of A. aegypti oviposition with 100 ppm of essential oil P. betle L. was 5 times lower than the control. Conclusion: This study demonstrated clearly that essential oil derived from P. betle L. potentially acts as alternate bioinsecticide to control A. aegypti population. The application can be varied or combined in different stages of mosquito development.

Download Full-text