Difference in expression of collagen type I and matrix metalloproteinase-1 in uterosacral ligaments of women with and without pelvic organ prolapse

2011 ◽  
Vol 155 (2) ◽  
pp. 225-228 ◽  
Author(s):  
Marko Vulic ◽  
Tomislav Strinic ◽  
Snjezana Tomic ◽  
Vesna Capkun ◽  
Ivana Alujevic Jakus ◽  
...  
Keyword(s):  
Pelvic Organ Prolapse ◽  
Matrix Metalloproteinase ◽  
Collagen Type ◽  
Collagen Type I ◽  
Pelvic Organ ◽  
Type I ◽  
Uterosacral Ligaments ◽  
Matrix Metalloproteinase 1

scholarly journals Ηλεκτροφυσιολογικές και ηλεκτροανατομικές ιδιότητες του δεξιού κόλπου σε ασθενείς με εμμένουσα κολπική μαρμαρυγή

2015 ◽  
Author(s):  
Χρήστος Γουδής
Keyword(s):  
Matrix Metalloproteinases ◽  
Matrix Metalloproteinase ◽  
Collagen Type ◽  
Collagen Type I ◽  
Type I ◽  
Tissue Inhibitor ◽  
Matrix Metalloproteinase 1

Σκοπός: Η κολπική μαρμαρυγή σχετίζεται με παθολογικό κολπικό υπόστρωμα. Στην παρούσα μελέτη διερευνήθηκε αν ασθενείς με μονήρη εμμένουσα κολπική μαρμαρυγή και ασθενείς με εμμένουσα κολπική μαρμαρυγή και μη ισχαιμική διατατική μυοκαρδιοπάθεια παρουσιάζουν διαφορές στις ηλεκτροφυσιολογικές και ηλεκτροανατομικές ιδιότητες του δεξιού κόλπου και στους βιοχημικούς δείκτες των μεταβολών του κολλαγόνου. Επίσης διερευνήθηκε η σχέση μεταξύ των δυναμικών του δεξιού κόλπου και των βιοχημικών δεικτών μεταβολών του κολλαγόνου στις δύο ομάδες.Μέθοδοι: Δέκα ασθενείς με εμμένουσα μονήρη κολπική μαρμαρυγή και οκτώ ασθενείς με εμμένουσα κολπική μαρμαρυγή και μη ισχαιμική διατατική μυοκαρδιοπάθεια συμπεριλήφθηκαν στη μελέτη. Προσδιορίστηκαν οι χρόνοι ανάνηψης του φλεβοκόμβου και οι δραστικές ανερέθιστες περίοδοι σε μήκη κύκλου 600ms, 500ms και 400ms, στο υψηλό και χαμηλό πλάγιο τοίχωμα δεξιού κόλπου, στον εγγύ στεφανιαίο κόλπο και δεξιό κολπικό ωτίο, και χαρτογραφήθηκε ο δεξιός κόλπος. Το αμινοτελικό προπεπτίδιο του κολλαγόνου τύπου Ι (serum N-terminal propeptide of collagen type I-PINP) και ο ιστικός αναστολέας της μεταλλοπρωτεϊνάσης-1 (tissue inhibitor of matrix metalloproteinases, TIMP-1), υπολογίστηκαν ως βιοχημικοί δείκτες σύνθεσης του κολλαγόνου τύπου Ι, ενώ το διασταυρούμενο καρβοξυτελικό τελοπεπτίδιο του κολλαγόνου τύπου Ι (cross-linked C-terminal telopeptide of collagen type I-CTx) και η μεταλλοπρωτεϊνάση-1 (matrix metalloproteinase-1, MMP-1) ως βιοχημικοί δείκτες αποδόμησης του κολλαγόνου τύπου Ι μεταξύ των δύο ομάδων.Αποτελέσματα: Δε διαπιστώθηκε σημαντική διαφορά στους χρόνους ανάνηψης του φλεβοκόμβου, στις δραστικές ανερέθιστες περιόδους στο υψηλό και χαμηλό πλάγιο τοίχωμα δεξιού κόλπου σε μήκη κύκλου 600ms, στις δραστικές ανερέθιστες περιόδους στον εγγύ στεφανιαίο κόλπο και το δεξιό κολπικό ωτίο, στο μέσο δυναμικό του δεξιού κόλπου και στους βιοχημικούς δείκτες μεταβολών του κολλαγόνου μεταξύ των δύο ομάδων. Σε ασθενείς με μονήρη εμμένουσα κολπική μαρμαρυγή τα επίπεδα ορού του TIMP-1 σχετίζονται με τα μέσα δυναμικά στο υψηλό πλάγιο και υψηλό οπίσθιο τοίχωμα του δεξιού κόλπου. Συμπέρασμα: Ασθενείς με μονήρη εμμένουσα κολπική μαρμαρυγή και ασθενείς με εμμένουσα κολπική μαρμαρυγή και μη ισχαιμκή διατατική μυοκαρδιοπάθεια παρουσιάζουν παρόμοιες μεταβολές στις ηλεκτροφυσιολογικές και ηλεκτροανατομικές ιδιότητες του δεξιού κόλπου, καθώς και παρόμοιες δομικές μεταβολές. Επιπλέον, ο βιοχημικος δείκτης της σύνθεσης κολλαγόνου TIMP-1 σχετίζεται με τα μέσα δυναμικά στο υψηλό πλάγιο και υψηλό οπίσθιο τοίχωμα του δεξιού κόλπου σε ασθενείς με μονήρη εμμένουσα κολπική μαρμαρυγή.

Type I collagen and matrix metalloproteinase 1, 3 and 9 gene polymorphisms in the predisposition to pelvic organ prolapse

2011 ◽  
Vol 285 (6) ◽  
pp. 1581-1586 ◽  
Author(s):  
Maria Maddalena Ferrari ◽  
Gabriela Rossi ◽  
Maria Luisa Biondi ◽  
Paola Viganò ◽  
Chiara Dell’Utri ◽  
...  
Keyword(s):  
Pelvic Organ Prolapse ◽  
Matrix Metalloproteinase ◽  
Gene Polymorphisms ◽  
Type I Collagen ◽  
Pelvic Organ ◽  
Type I ◽  
Matrix Metalloproteinase 1

Investigation of the effects of prostaglandin E2 on equine superficial digital flexor tendon fibroblasts in vitro

2010 ◽  
Vol 23 (06) ◽  
pp. 417-423 ◽  
Author(s):  
J. M. Cissell ◽  
S. C. Milton ◽  
L. A. Dahlgren
Keyword(s):  
Gene Expression ◽  
Matrix Protein ◽  
Collagen Type ◽  
Flexor Tendon ◽  
Cell Metabolism ◽  
Collagen Type I ◽  
Type I ◽  
Matrix Metalloproteinase 1 ◽  
Superficial Digital Flexor Tendon

Summary Objectives: To evaluate the effects of pros-taglandin E2 (PGE2) treatment on the metabolism of equine tendon fibroblasts in vitro to aid in investigating the response of tendon fibroblasts to injury and novel therapeutics. Methods: Superficial digital flexor tendon fibroblasts isolated via collagenase digestion from six young adult horses were grown in monolayer in four concentrations of PGE2 (0, 10, 50, 100 ng/ml) for 48 hours. Cells and medium were harvested for gene expression (collagen types I and III, cartilage oligomeric matrix protein [COMP], decorin, and matrix metalloproteinase-1, –3, and –13), biochemical analysis (glycosaminoglycan, DNA, and collagen content), and cytological staining. Results: Gene expression for collagen type I was significantly increased at 100 ng/ml PGE2 compared to 10 and 50 ng/ml. There were not any significant differences detected for gene expression of collagen type III, COMP or dec-orin or for biochemical content and cell morphology. Clinical significance: Under the conditions investigated, exogenous treatment of equine tendon fibroblasts with PGE2 failed to alter cell metabolism in a manner useful as a model of tendon injury. A model that applies cyclic strain to a three dimensional construct seeded with tendon fibroblasts may prove to be a more useful model and merits further investigation for this purpose. The ability to assess cellular responses in an environment where the cells are supported within the extracellular matrix may prove beneficial.

scholarly journals Antifibrotic cardioprotection of berberine via downregulating myocardial IGF-1 receptor-regulated MMP-2/MMP-9 expression in diabetic rats

2018 ◽  
Vol 315 (4) ◽  
pp. H802-H813 ◽  
Author(s):  
Guohua Li ◽  
Wenjuan Xing ◽  
Min Zhang ◽  
Fenghao Geng ◽  
Hongyan Yang ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
High Glucose ◽  
Cardiac Fibrosis ◽  
Collagen Type ◽  
Cardiac Fibroblasts ◽  
Collagen Type I ◽  
Diabetic Rats ◽  
Type I ◽  
Antifibrotic Effect

Diabetic cardiac fibrosis increases ventricular stiffness and facilitates the occurrence of diastolic dysfunction. Our previous studies have shown that berberine, a natural alkaloid, attenuates cardiac ischemia-reperfusion injury in diabetic rats. The aim of present study was to investigate the effects of long-term berberine treatment on cardiac remodeling in diabetic rats and the underlying mechanisms. Diabetic rats induced by low-dose streptozotocin injection combined with 8 wk of high-fat diet displayed significant cardiac matrix collagen deposition and dysfunction, whereas berberine administration (200 mg·kg−1·day−1, gavage 4 wk) significantly ameliorated cardiac fibrosis and dysfunction and reduced cardiac IGF-1 receptor (IGF-1R) expression in diabetic rats. Interestingly, IGF-1R expression was upregulated in cardiac fibroblasts isolated from diabetic hearts or cultured in high-glucose conditions (30 mM). High glucose treatment or IGF-1R overexpression increased matrix metalloproteinase (MMP)-2/MMP-9 expression, α-smooth muscle actin (α-SMA), and collagen type I expression in cardiac fibroblasts. In contrast, berberine treatment significantly inhibited IGF-1R expression and exerted an antifibrotic effect in high glucose-cultured cardiac fibroblasts, as manifested by decreased MMP-2/MMP-9, α-SMA, and collagen type I expression, whereas IGF-1R siRNA plus berberine treatment did not further enhance this antifibrotic effect compared with berberine treatment alone. Taken together, long-term berberine treatment ameliorates cardiac fibrosis and dysfunction by downregulating IGF-1R expression in cardiac fibroblasts and subsequently reducing MMP-2/MMP-9, α-SMA, and collagen type I expression in diabetic hearts. The findings suggest the therapeutic potential of berberine for diabetic cardiomyopathy associated with cardiac fibrosis. NEW & NOTEWORTHY Berberine downregulated IGF-1 receptor expression and matrix metalloproteinase-2/matrix metalloproteinase-9 levels in cardiac fibroblasts and thus inhibited fibroblast differentiation and collagen overproduction in diabetic hearts, suggesting a novel mechanism for antifibrotic cardioprotection of berberine in type 2 diabetes.

Increased expression of matrix metalloproteinase-1 in uterosacral ligament tissue from women with pelvic organ prolapse

2010 ◽  
Vol 89 (6) ◽  
pp. 832-834 ◽  
Author(s):  
Tomislav Strinic ◽  
Marko Vulic ◽  
Snjezana Tomic ◽  
Vesna Capkun ◽  
Ivica Stipic ◽  
...  
Keyword(s):  
Pelvic Organ Prolapse ◽  
Matrix Metalloproteinase ◽  
Pelvic Organ ◽  
Uterosacral Ligament ◽  
Matrix Metalloproteinase 1

Temporal extracellular matrix adaptations in ligament during wound healing and hindlimb unloading

2007 ◽  
Vol 293 (4) ◽  
pp. R1552-R1560 ◽  
Author(s):  
D. A. Martinez ◽  
A. C. Vailas ◽  
R. Vanderby ◽  
R. E. Grindeland
Keyword(s):  
Gene Expression ◽  
Extracellular Matrix ◽  
Wound Healing ◽  
Matrix Metalloproteinase ◽  
Collagen Type ◽  
Male Rats ◽  
Matrix Metalloproteinase 2 ◽  
Type Iii Collagen ◽  
Type I ◽  
Matrix Metalloproteinase 1

Previous data from spaceflight studies indicate that injured muscle and bone heal slowly and abnormally compared with ground controls, strongly suggesting that ligaments or tendons may not repair optimally as well. Thus the objective of this study was to investigate the biochemical and molecular gene expression of the collagen extracellular matrix in response to medial collateral ligament (MCL) injury repair in hindlimb unloaded (HLU) rodents. Male rats were assigned to 3- and 7-wk treatment groups with three subgroups each: sham control, ambulatory healing (Amb-healing), and HLU-healing groups. Amb- and HLU-healing animals underwent bilateral surgical transection of their MCLs, whereas control animals were subjected to sham surgeries. All surgeries were performed under isoflurane anesthesia. After 3 wk or 7 wk of HLU, rats were euthanized and MCLs were surgically isolated and prepared for molecular or biochemical analyses. Hydroxyproline concentration and hydroxylysylpyridinoline collagen cross-link contents were measured by HPLC and showed a substantial decrement in surgical groups. MCL tissue cellularity, quantified by DNA content, remained significantly elevated in all HLU-healing groups vs. Amb-healing groups. MCL gene expression of collagen type I, collagen type III, collagen type V, fibronectin, decorin, biglycan, lysyl oxidase, matrix metalloproteinase-2, and tissue inhibitor of matrix metalloproteinase-1, measured by real-time quantitative PCR, demonstrated differential expression in the HLU-healing groups compared with Amb-healing groups at both the 3- and 7-wk time points. Together, these data suggest that HLU affects dense fibrous connective tissue wound healing and confirms previous morphological and biomechanical data that HLU inhibits the ligament repair processes.

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