Investigation of nanocapsules stabilization by amorphous excipients during freeze-drying and storage

SummaryIn this paper an inhibitor is described that is found in hemophilic plasma and serum different from any till now described inhibitor. The inhibitor only inhibits prothrombin activation in the “intrinsic clotting systems”. This inhibitor is probably not present in normal human plasma or serum. It is destroyed by ether and freeze drying, is labile to acid and storage at room temperature. It is stable upon dialysis and has not been adsorbed on barium sulfate, aluminum hydroxide or kaolin. It precipitates at 50% v/v saturation with alcohol. The nature of this inhibitor seems to be a protein or lipoprotein.Factor VIII was isolated from hemophilic plasma. The amount isolated was the same as from normal plasma and the activity properties were not different. Hemophiliacs have normal amounts of factor VIII.

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Effect of additives on freeze-drying and storage of Yarrowia lipolytica lipase

Journal of Biotechnology ◽

10.1016/j.jbiotec.2010.09.396 ◽

2010 ◽

Vol 150 ◽

pp. 352-352 ◽

Cited By ~ 1

Author(s):

F. Darvishi ◽

J. Destain ◽

I. Nahvi ◽

P. Thonart ◽

H. Zarkesh-Esfahani

Keyword(s):

Yarrowia Lipolytica ◽

Freeze Drying ◽

Effect Of Additives ◽

And Storage

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Optimising storage conditions and processing of sheep urine for nitrogen cycle and gaseous emission measurements from urine patches

Scientific Reports ◽

10.1038/s41598-021-91498-4 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Alice F. Charteris ◽

Karina A. Marsden ◽

Jess R. Evans ◽

Harry A. Barrat ◽

Nadine Loick ◽

...

Keyword(s):

Freeze Drying ◽

Storage Conditions ◽

Chemical Components ◽

Gaseous Emissions ◽

Gaseous Emission ◽

Grazing Systems ◽

And Storage ◽

Sheep Urine ◽

Country Specific

AbstractIn grazing systems, urine patches deposited by livestock are hotspots of nutrient cycling and the most important source of nitrous oxide (N2O) emissions. Studies of the effects of urine deposition, including, for example, the determination of country-specific N2O emission factors, require natural urine for use in experiments and face challenges obtaining urine of the same composition, but of differing concentrations. Yet, few studies have explored the importance of storage conditions and processing of ruminant urine for use in subsequent gaseous emission experiments. We conducted three experiments with sheep urine to determine optimal storage conditions and whether partial freeze-drying could be used to concentrate the urine, while maintaining the constituent profile and the subsequent urine-derived gaseous emission response once applied to soil. We concluded that filtering of urine prior to storage, and storage at − 20 °C best maintains the nitrogen-containing constituent profile of sheep urine samples. In addition, based on the 14 urine chemical components determined in this study, partial lyophilisation of sheep urine to a concentrate represents a suitable approach to maintain the constituent profile at a higher overall concentration and does not alter sheep urine-derived soil gaseous emissions.

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Effect of Additives on Freeze-Drying and Storage of Yarrowia lipolytica Lipase

Applied Biochemistry and Biotechnology ◽

10.1007/s12010-012-9844-z ◽

2012 ◽

Vol 168 (5) ◽

pp. 1101-1107 ◽

Cited By ~ 19

Author(s):

Farshad Darvishi ◽

Jacqueline Destain ◽

Iraj Nahvi ◽

Philippe Thonart ◽

Hamid Zarkesh-Esfahani

Keyword(s):

Yarrowia Lipolytica ◽

Freeze Drying ◽

Effect Of Additives ◽

And Storage

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Stability of pseudorabies virus during freeze-drying and storage: effect of suspending media

Journal of Clinical Microbiology ◽

10.1128/jcm.4.1.1-5.1976 ◽

1976 ◽

Vol 4 (1) ◽

pp. 1-5

Author(s):

E M Scott ◽

W Woodside

Keyword(s):

Freeze Drying ◽

Pseudorabies Virus ◽

Storage Effect ◽

Drying Process ◽

Sodium Glutamate ◽

Degree Of Protection ◽

The Stability ◽

Subsequent Storage ◽

And Storage ◽

Lactalbumin Hydrolysate

The effect of suspending media on the stability of pseudorabies virus upon freeze-drying and subsequent storage was studied. A variety of media was tested, including: sodium glutamate; sucrose; lactose; lactalbumin hydrolysate; peptone; a combination of sucrose, dextran, and glutamate; and various combinations of sucrose, glutamate, and potassium phosphates. Suspending media containing glutamate, either alone or in combination with sucrose and either dextran or phosphates, afforded the greatest degree of protection during the freeze-drying process and upon storage. Some possible functions of these additives in preventing injury to the virus during freezing and drying have been suggested.

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