scholarly journals Lecithin-based nanoemulsions of traditional herbal wound healing agents and their effect on human skin cells

2021 ◽  
Author(s):  
Claudia Vater ◽  
Leonie Bosch ◽  
Alexandra Mitter ◽  
Thomas Göls ◽  
Saskia Seiser ◽  
...  
Keyword(s):  
Wound Healing ◽  
Human Skin ◽  
Skin Cells ◽  
Human Skin Cells

Enhanced extracellular matrix synthesis using collagen dressings loaded with Artemisia absinthium plant extract

2018 ◽  
Vol 33 (5) ◽  
pp. 516-528 ◽  
Author(s):  
Alexandra Gaspar-Pintiliescu ◽  
Ana-Maria Seciu ◽  
Florin Miculescu ◽  
Lucia Moldovan ◽  
Elena Ganea ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Wound Healing ◽  
Human Skin ◽  
Composite Scaffold ◽  
Dermal Fibroblasts ◽  
Environment Interaction ◽  
High Porosity ◽  
Skin Cells ◽  
Extracellular Matrix Components ◽  
Human Skin Cells

The aim of this study was to develop three-dimensional porous composites of collagen (Col) incorporating polyphenolic-rich wormwood extract and to investigate their interaction with human skin cells, in order to optimize wound healing treatments. The scaffolds’ ultrastructure was observed by scanning electron microscopy, and biodegradability and bioactive compounds release were investigated in physiologic environment. Interaction of composites in direct and indirect contact with human skin cells was evaluated using two in vitro experimental models. ColWE scaffolds presented high porosity, swelling degree, and increased stability against enzymatic degradation, compared to Col scaffold. Composite scaffolds incorporating higher quantities of wormwood extract allowed better control of polyphenolics release. ColWE 0.5 variant favored the attachment and proliferation of human dermal fibroblasts and keratinocyte cells. In addition, the composite scaffold stimulated the synthesis of skin extracellular matrix components. All these results demonstrated that ColWE composites with improved physico-chemical and biological properties could be used in advanced wound healing applications.

scholarly journals 690 The influence of octenidine dihydrochloride on human skin cells and wound healing

2017 ◽  
Vol 137 (10) ◽  
pp. S311
Author(s):  
N. Nikolić ◽  
P. Kienzl ◽  
P. Tajpara ◽  
M. Mildner ◽  
J. Matiasek ◽  
...  
Keyword(s):  
Wound Healing ◽  
Human Skin ◽  
Skin Cells ◽  
Octenidine Dihydrochloride ◽  
Human Skin Cells

Wound Healing Effect of Conditioned Media Obtained From Adipose Tissue on Human Skin Cells

2016 ◽  
Vol 77 (2) ◽  
pp. 156-163 ◽  
Author(s):  
Johanna Kober ◽  
Alfred Gugerell ◽  
Melanie Schmid ◽  
Maximilian Zeyda ◽  
Elisabeth Buchberger ◽  
...  
Keyword(s):  
Wound Healing ◽  
Adipose Tissue ◽  
Human Skin ◽  
Conditioned Media ◽  
Skin Cells ◽  
Healing Effect ◽  
Wound Healing Effect ◽  
Human Skin Cells

scholarly journals Exogenous Antioxidants Impact on UV-Induced Changes in Membrane Phospholipids and the Effectiveness of the Endocannabinoid System in Human Skin Cells

Antioxidants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1260
Author(s):  
Agnieszka Gęgotek ◽  
Anna Jastrząb ◽  
Marta Dobrzyńska ◽  
Michał Biernacki ◽  
Elżbieta Skrzydlewska
Keyword(s):  
Ascorbic Acid ◽  
Cell Viability ◽  
Human Skin ◽  
Phospholipid Fatty Acid ◽  
Antioxidant Properties ◽  
Endocannabinoid System ◽  
Membrane Phospholipids ◽  
Skin Cells ◽  
Human Skin Cells ◽  
Induced Changes

Natural antioxidants effectively counteract changes caused by UV radiation in human skin cells. However, their action is limited due to their lipo/hydrophilicity. Therefore, the aim of this study was to analyze the mutual protective action of hydrophilic ascorbic acid and partially lipophilic rutin against UVA/UVB-induced changes in membranes phospholipid and endocannabinoid system in keratinocytes and fibroblasts. Obtained results clearly showed that, despite the stronger antioxidant properties of ascorbic acid, the lipid membranes were more effectively protected against UV-induced oxidation by rutin, including changes in phospholipid fatty acid levels, prevention against reactive aldehydes formation and endocannabinoids degradation. Ascorbic acid more strongly prevented UV-induced endocannabinoid receptors expression in fibroblasts, especially CB1. However, the combined action of used antioxidants resulted in the greatest cytoprotective effect, which was evident in the inflammatory marker TNFα down-regulation and increased cell viability following cell irradiation. The applied mixture of antioxidants showed a stronger protective in relation to membrane phospholipids in keratinocytes and in the endocannabinoid system in fibroblasts. In conclusion, it can be suggested that combined antioxidant capacities of ascorbic acid and rutin protects against lipid peroxidation but also decreases the UV-induced inflammation by direct interaction with the endocannabinoid system, thus increasing skin cell viability.

Basic Red 51, a permitted semi-permanent hair dye, is cytotoxic to human skin cells: Studies in monolayer and 3D skin model using human keratinocytes (HaCaT)

2014 ◽  
Vol 227 (2) ◽  
pp. 139-149 ◽  
Author(s):  
Thalita B. Zanoni ◽  
Manoela Tiago ◽  
Fernanda Faião-Flores ◽  
Silvia B. de Moraes Barros ◽  
Aalt Bast ◽  
...  
Keyword(s):  
Human Skin ◽  
Human Keratinocytes ◽  
Skin Model ◽  
Hair Dye ◽  
Skin Cells ◽  
Human Skin Cells ◽  
3D Skin
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