scholarly journals Application of artificial substrate samplers to assess enrichment of metals of concern by river floodwaters to lakes across the Peace-Athabasca Delta

2021 ◽  
Vol 38 ◽  
pp. 100954
Author(s):  
Cory A.M. Savage ◽  
Tanner Owca ◽  
Mitchell L. Kay ◽  
Jelle Faber ◽  
Brent B. Wolfe ◽  
...  
Keyword(s):  
Author(s):  
G. D. Gagne ◽  
M. F. Miller

We recently described an artificial substrate system which could be used to optimize labeling parameters in EM immunocytochemistry (ICC). The system utilizes blocks of glutaraldehyde polymerized bovine serum albumin (BSA) into which an antigen is incorporated by a soaking procedure. The resulting antigen impregnated blocks can then be fixed and embedded as if they are pieces of tissue and the effects of fixation, embedding and other parameters on the ability of incorporated antigen to be immunocyto-chemically labeled can then be assessed. In developing this system further, we discovered that the BSA substrate can also be dried and then sectioned for immunolabeling with or without prior chemical fixation and without exposing the antigen to embedding reagents. The effects of fixation and embedding protocols can thus be evaluated separately.


1970 ◽  
Vol 23 (02) ◽  
pp. 306-312 ◽  
Author(s):  
D Nyman

SummaryA method for preparation of an artificial substrate for the one-stage factor VIII assay is described. Standardization of the test is given. The method is specific and reproducible.


1987 ◽  
Vol 35 (8) ◽  
pp. 909-916 ◽  
Author(s):  
G D Gagne ◽  
M F Miller

We describe an artificial substrate system for optimization of labeling parameters in electron microscope immunocytochemical studies. The system involves use of blocks of glutaraldehyde-polymerized BSA into which a desired antigen is incorporated by a simple soaking procedure. The resulting antigen-impregnated artificial substrate can then be fixed and embedded identically to a piece of tissue. The BSA substrate can also be dried and then sectioned for immunolabeling with or without chemical fixation and without exposing the antigen to dehydrating agents and embedding resins. The effects of various fixation and embedding procedures can thus be evaluated separately. Other parameters affecting immunocytochemical labeling, such as antibody and conjugate concentration, can also be evaluated. We used this system, along with immunogold labeling, to determine quantitatively the optimal fixation and embedding conditions for labeling of hepatitis B surface antigen (HbsAg), human IgG, and horseradish peroxidase. Using unfixed and unembedded HBsAg, we were able to detect antigen concentrations below 20 micrograms/ml. We have shown that it is not possible to label HBsAg within resin-embedded cells using conventional aldehyde fixation protocols and polyclonal antibodies.


1986 ◽  
Vol 15 (11) ◽  
pp. 1885-1888 ◽  
Author(s):  
Yuji Maki ◽  
Gotaro Watanabe ◽  
Akio Saito ◽  
Tanetoshi Koyama ◽  
Kyozo Ogura

2021 ◽  
Vol 25 (5) ◽  
pp. 39-45
Author(s):  
A.Kh. Sariev ◽  
N.Yu. Cherbakova ◽  
N.Yu. Terentyeva

The assessment of the possibility of creating a plant-soil cover on an artificially created seal-gravel drainage layer with a rocky bed (h=110¸150 cm), on the disturbed areas of the adjacent areas of tailings storage facilities. Research for 2017–2019 shows that when sowing recult-vants with sowing standards of 150–200 kg/ha and doses of fertilizer N60P60K60 and N120P60K60 on an artificial substrate it is possible to form meadow formations with a density of grass 10–12000 pc/m2 and average productivity of 21–24 c/ha. A plant-soil-blooded blood with a turf layer of 8–10 cm is created with the simultaneous overlap of heavy metals of man-made substrates and elimination of pulp sands dusting, which contributes to the improvement of the microclimate of the environment.


1996 ◽  
Vol 251 (1) ◽  
pp. 53-64 ◽  
Author(s):  
Hideto Shibata ◽  
Tatsuhiko Yagi
Keyword(s):  

Author(s):  
MI Hoque ◽  
AKMF Rahman ◽  
MA Mansur ◽  
S Rahman

An experiment was carried out on the effects of periphyton on monoculture of Thai sharputi, Puntius gonionotus at the Department of Fisheries Management, Bangladesh Agricultural University, Mymensingh during 7th August to 8th November. In treatment-1 bamboo poles were used as artificial substrate for periphyton production and in treatment-2 there was no artificial substrate (control). Each of the six ponds was stocked with 150 fingerlings of average size 6.41 cm and 3.60 g. The ponds were fertilized fortnightly with manure (cow dung) at a rate of 10 kg decimal-1, urea 60 g decimal-1 and triple super phosphate 90 g decimal-1. During the experimental period, the ranges of physico-chemical parameters viz. air temperature (31.0-35.50C), water temperature (29-320C), water depth (0.56-0.84 m), transparency (32-63 cm), dissolved oxygen (3.5-7.8 mg L-1), pH (6.8-7.9), total alkalinity (44-92 mg L-1), free CO2 (1.5-4.0 mg L-1), phosphate-phosphorus (0.31-1.07 mg L-1) and nitrate-nitrogen (1.12-2.30 mg L-1) were within the productive range and more or less similar in the ponds under treatments-1 and 2. Among the observed biological parameters, there were 35 genera of phytoplankton composed of five groups and 13 genera of zooplankton composed of four groups in the experimental ponds. Thirty three genera under the groups of Bacillariophyceae, Chlorophyceae, Cyanophyceae and Euglenophyceae formed the periphyton on bamboo poles in the experimental ponds. Net fish production of the ponds with periphyton under treatment-1 was about 1.5 times higher than those of the ponds without periphyton (treatment-2). By analysis of variance, it was found that the net fish production of Thai sharputi under treatment-1 was significantly higher than that under treatment-2 (p< 0.05). Finally, it can be concluded that periphyton is one of the preferable food item of Thai sharputi and it is also suggested that growth and production of Thai sharputi can be increased if arrangement is made for periphyton production. Int. J. Agril. Res. Innov. & Tech. 8 (2): 13-23, December, 2018


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