Seaweed Lobophora variegata-based Silver Nanopesticide for environmental friendly management of economically important pest, Spodoptera litura

2021 ◽  
pp. 100531
Author(s):  
Kitherian Sahayaraj ◽  
Thangapandi Veni ◽  
Jesu Antony Martin Rathi
Keyword(s):  
Spodoptera Litura ◽  
Lobophora Variegata ◽  
Environmental Friendly ◽  
Important Pest

scholarly journals A systemic study of indoxacarb resistance in Spodoptera litura revealed complex expression profiles and regulatory mechanism

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Li Shi ◽  
Yao Shi ◽  
Ya Zhang ◽  
Xiaolan Liao
Keyword(s):  
Spodoptera Litura ◽  
Molecular Mechanisms ◽  
Target Genes ◽  
Expression Profiles ◽  
Resistance Mechanisms ◽  
Differentially Expressed ◽  
Glutathione S Transferase ◽  
Differentially Expressed Mirnas ◽  
Important Pest ◽  
Resistant Strains

Abstract The tobacco cutworm, Spodoptera litura, is an important pest of crop and vegetable plants worldwide, and its resistance to insecticides have quickly developed. However, the resistance mechanisms of this pest are still unclear. In this study, the change in mRNA and miRNA profiles in the susceptible, indoxacarb-resistant and field indoxacarb-resistant strains of S. litura were characterized. Nine hundred and ten co-up-regulated and 737 co-down-regulated genes were identified in the resistant strains. Further analysis showed that 126 co-differentially expressed genes (co-DEGs) (cytochrome P450, carboxy/cholinesterase, glutathione S-transferase, ATP-binding cassette transporter, UDP-glucuronosyl transferase, aminopeptidase N, sialin, serine protease and cuticle protein) may play important roles in indoxacarb resistance in S. litura. In addition, a total of 91 known and 52 novel miRNAs were identified, and 10 miRNAs were co-differentially expressed in the resistant strains of S. litura. Furthermore, 10 co-differentially expressed miRNAs (co-DEmiRNAs) had predicted co-DEGs according to the expected miRNA-mRNA negative regulation pattern and 37 indoxacarb resistance-related co-DEGs were predicted to be the target genes. These results not only broadened our understanding of molecular mechanisms of insecticide resistance by revealing complicated profiles, but also provide important clues for further study on the mechanisms of miRNAs involved in indoxacarb resistance in S. litura.

scholarly journals Efication of Some Entomopatogen Fungus on Green Ladybug Imago (Nezara Viridula Linnaeus) (Hemiptera: Pentatomidae)

2019 ◽  
Vol 1 (2) ◽  
Author(s):  
Muhammad Agung Permadi ◽  
Rafiqah Amanda Lubis ◽  
Mukhlis Mukhlis ◽  
Imam Aryo Pahlawan ◽  
Sulaiman Abdi
Keyword(s):  
Mortality Rate ◽  
Entomopathogenic Fungi ◽  
Entomopathogenic Fungus ◽  
Body Part ◽  
Nezara Viridula ◽  
Fungal Mycelium ◽  
Environmental Friendly ◽  
Randomized Design ◽  
Important Pest ◽  
Completely Randomized Design

Green ladybug (Nezara viridula L.) is an important pest in some plants, including soybeans. N. viridula attacks cause a decrease in yield of up to 80%. Environmental friendly pest control studies are needed to overcome the problem of resurgence caused by N. viridula, among others by utilizing entomopathogenic fungus as their natural enemies. This study discusses about the efficacy of entomopathogenic fungi against N. viridula imago. The research is conducted at the Agricultural Agrotechnology Laboratory, Muhammadiyah University of South Tapanuli. The study uses a completely randomized design, nine treatments (control and eight isolates of entomopathogenic fungus) with three replications. The conidia density used is 108 conidia / ml. The entomopathogenic fungus application was performed on N. viridula imago. The results shows that MetKP fungus isolates are able to kill N. viridula imago with the highest mortality rate of 78.33%. The isolates of MetKM fungi and M. anisopliae caused the lowest imago mortality of 61.67%. Isolate of B. bassiana fungus had the lowest LT50 with a time of 5.66 HSA and the isolate of the MetTmM fungus had the highest LT50 with a time of 7.27 HSA. In the immature body part of N. viridula infected with fungus is enveloped by entomopathogenic fungal mycelium.

scholarly journals Compatibility of Lemongrass Extract with Spodoptera litura Nuclear Polyhedrosis Virus Against Spodoptera litura on Soybean Plants

2021 ◽  
Vol 2 (1) ◽  
pp. 7-13
Author(s):  
Nafisatul Afidah ◽  
Tutung Hadiastono ◽  
Bedjo Bedjo ◽  
Fery Abdul Choliq
Keyword(s):  
Spodoptera Litura ◽  
Biocontrol Agent ◽  
Nuclear Polyhedrosis Virus ◽  
Randomized Design ◽  
Important Pest ◽  
Completely Randomized Design ◽  
Polyhedrosis Virus ◽  
Soybean Plants ◽  
Nuclear Polyhedrosis ◽  
Tuber Crops

Armyworm (Spodoptera litura) is an important pest that damages the leaves of soybean. SlNPV is one type of pathogen that has potential as a biocontrol agent to control armyworms because it is specific, selective, and effective. This study aimed to investigate the compatibility between the lemongrass and SlNPV in controlling armyworm. The Research was conducted at the Laboratory of Indonesian Legumes and Tuber Crops Research Institute (ILETRI) from February 2016 until April 2016. The study using completely randomized design with 7 treatments and 3 replication with a treatment that consists of control, SlNPV-JTM 97C 1,5 × 106 PIB/ml, 10% of lemongrass extract, a combination SlNPV-JTM 97C fixed concentrate of 1,5 × 106 PIB/ml with 2.5%, 5%, 7.5%, and 10% of lemongrass extract. The results showed that the combination of SlNPV-JTM 97C 1.5 × 106 PIB/ml with 10% lemongrass had the highest level of virulence, with 96% of mortality on larva 100% on the pupa stage, so that the combination of SlNPV and lemongrass extracts was compatible in controlling S.litura.

Negative cross resistance of Spodoptera litura Fabricius population of tomato to newer molecule spinetoram

ENTOMON ◽  
2019 ◽  
Vol 44 (2) ◽  
pp. 127-132
Author(s):  
M. Visnupriya ◽  
N. Muthukrishnan
Keyword(s):  
Spodoptera Litura ◽  
Laboratory Strain ◽  
Field Population ◽  
Resistance Ratio ◽  
Cross Resistance ◽  
In Vivo Toxicity

Field population of Spodoptera litura from tomato ( resistant to the majority of the conventional insecticide molecules) were subjected to the in vivo toxicity of spinetoram 12 SC to assess whether cross resistance exists or not. Untreated larvae of both field and laboratory strains showed no mortality during 48 hours of feeding. After 48 hours of feeding on spinetoram 12 SC treated leaves, LC50s of field larvae were 0.28, 0.93, 3.71 and 7.11 ppm for the 2nd, 3rd, 4th and 5th instars of S. litura respectively. However, in the laboratory strain these values were 1.12, 5.86, 36.72 and 91.55 ppm for 2nd, 3rd, 4th and 5th instars of S. litura respectively. Resistance ratio was 0.25, 0.16, 0.10 and 0.08 for the 2nd instar up to the 5th instar of S. litura.

Production of Asbestos-free Brake Pad Using Groundnut Shell as Filler Material

2018 ◽  
Vol 4 (12) ◽  
Author(s):  
W. C. Solomon ◽  
M. T. Lilly ◽  
J. I. Sodiki
Keyword(s):  
Phenolic Resin ◽  
Cost Effective ◽  
Filler Material ◽  
Particle Sizes ◽  
Brake Pad ◽  
Brake Pads ◽  
Environmental Friendly ◽  
Sieve Size ◽  
Groundnut Shell ◽  
The Cost

The development and evaluation of brake pads using groundnut shell (GS) particles as substitute material for asbestos were carried out in this study. This was with a view to harnessing the properties of GS, which is largely deposited as waste, and in replacing asbestos which is carcinogenic in nature despite its good tribological and mechanical properties. Two sets of composite material were developed using varying particle sizes of GS as filler material, with phenolic resin as binder with percentage compositions of 45% and 50% respectively. Results obtained indicate that the compressive strength and density increase as the sieve size of the filler material decreases, while water and oil absorption rates increase with an increase in sieve size of GS particle. This study also indicates that the cost of producing brake pad can be reduced by 19.14 percent if GS is use as filler material in producing brake pad. The results when compared with those of asbestos and industrial waste showed that GS particle can be used as an effective replacement for asbestos in producing automobile brake pad. Unlike asbestos, GS-based brake pads are environmental friendly, biodegradable and cost effective.

Peramalan Serangan Hama Tanaman Tembakau (Nicotiana tabaccum) pada Fase Vegetatif di PT. Tarutama Nusantara Jember

2020 ◽  
Author(s):  
Irma Harlianingtyas ◽  
◽  
Ramadhan Taufika ◽  
Keyword(s):  
Spodoptera Litura ◽  
Nicotiana Tabaccum

Tembakau adalah produk pertanian semusim yang bukan termasuk dalam komoditas perkebunan. Produk ini dikonsumsi bukan untuk makanan tetapi sebagai pengisi waktu luang yaitu sebagai bahan baku rokok dan cerutu. Tembakau juga dapat dikunyah. Kandungan metabolit sekunder yang kaya juga membuatnya bermanfaat sebagai pestisida dan bahan baku obat. Salah satu tantangan dalam budidaya tembakau adalah adanya organisme pengganggu tanaman. Serangan hama dan penyakit dapat mempengaruhi kualitas maupun kuantitas hasil panen tembakau. Serangan hama dan penyakit yang paling banyak terjadi adalah pada saat fase vegetatif, karena pada vase ini semua bagian tanaman menjadi sasaran makanan bagi organisme pengganggu tanaman. Organisme pengganggu tanaman yang dianggap sebagai hama pada vase vegetattif tanaman tembakau adalah ualt tanah, ulat daun, belalang, kupu, gayas/jangkrik. Kegiatan penelitian ini dilakukan di PT Tarutama Nusantara Jember, data yang digunakan adalah data serangan hama yang terjadi pada seluruh kebun milik TTN dari tahun 2013 hingga 2019. Tujuan dilakukan penelitian ini adalah mengtahui prediksi serangan hama yang akan terjadi guna mempermudah perusahaan dalam menentukan strategi dan kebijakan guna meningkatkan produksi tembakau khususnya sebagai pelapis cerutu dekblad/wrapper. Metode penelitian yang digunakan untuk memprediksi serangan hama yang terjadi adalah metode regresi linier. Hasil penelitian menunjukkan prediksi serangan hama ulat tanah (Agrotis iplison) tahun 2021 sebesar 15,6%, ulat grayak (Spodoptera litura) sebesar 19,08%, serangan hama belalang sebesar 15,77%, dan serangan hama ketep (Setomorpha rutella) sebesar 15,23%, serta serangan hama jangkrik (Gryllidae) pada tahun 2021 adalah sebesar 8,20%.

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