A WRKY transcription factor PbWRKY40 from Pyrus betulaefolia functions positively in salt tolerance and modulating organic acid accumulation by regulating PbVHA-B1 expression

2022 ◽  
pp. 104782
Author(s):  
Likun Lin ◽  
Kaili Yuan ◽  
Yongdan Huang ◽  
Huizhen Dong ◽  
Qinghai Qiao ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Salt Tolerance ◽  
Organic Acid ◽  
Wrky Transcription Factor ◽  
Acid Accumulation

scholarly journals Association of transcription factor WRKY56 gene from Populus simonii × P. nigra with salt tolerance in Arabidopsis thaliana

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7291 ◽  
Author(s):  
Lei Wang ◽  
Wenjing Yao ◽  
Yao Sun ◽  
Jiying Wang ◽  
Tingbo Jiang
Keyword(s):  
Transcription Factor ◽  
Salt Stress ◽  
Abiotic Stress ◽  
Salt Tolerance ◽  
Stress Responses ◽  
Germination Rate ◽  
Wrky Transcription Factor ◽  
Biotic And Abiotic Stress ◽  
Populus Simonii ◽  
Reading Frame

The WRKY transcription factor family is one of the largest groups of transcription factor in plants, playing important roles in growth, development, and biotic and abiotic stress responses. Many WRKY genes have been cloned from a variety of plant species and their functions have been analyzed. However, the studies on WRKY transcription factors in tree species under abiotic stress are still not well characterized. To understand the effects of the WRKY gene in response to abiotic stress, mRNA abundances of 102 WRKY genes in Populus simonii × P. nigra were identified by RNA sequencing under normal and salt stress conditions. The expression of 23 WRKY genes varied remarkably, in a tissue-specific manner, under salt stress. Since the WRKY56 was one of the genes significantly induced by NaCl treatment, its cDNA fragment containing an open reading frame from P. simonii × P. nigra was then cloned and transferred into Arabidopsis using the floral dip method. Under salt stress, the transgenic Arabidopsis over-expressed the WRKY56 gene, showing an increase in fresh weight, germination rate, proline content, and peroxidase and superoxide dismutase activity, when compared with the wild type. In contrast, transgenic Arabidopsis displayed a decrease in malondialdehyde content under salt stress. Overall, these results indicated that the WRKY56 gene played an important role in regulating salt tolerance in transgenic Arabidopsis.

Functional Analysis of a Novel Chrysanthemum WRKY Transcription Factor Gene Involved in Salt Tolerance

2013 ◽  
Vol 32 (1) ◽  
pp. 282-289 ◽  
Author(s):  
Qing-Lin Liu ◽  
Ke-Dong Xu ◽  
Yuan-Zhi Pan ◽  
Bei-Bei Jiang ◽  
Guang-Li Liu ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Transcription Factor ◽  
Salt Tolerance ◽  
Wrky Transcription Factor ◽  
Transcription Factor Gene ◽  
Factor Gene

A novel salt inducible WRKY transcription factor gene, AhWRKY75, confers salt tolerance in transgenic peanut

2021 ◽  
Vol 160 ◽  
pp. 175-183
Author(s):  
Hong Zhu ◽  
Yanan Jiang ◽  
Yue Guo ◽  
Jianbin Huang ◽  
Minghan Zhou ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Salt Tolerance ◽  
Wrky Transcription Factor ◽  
Transcription Factor Gene ◽  
Factor Gene ◽  
Transgenic Peanut

scholarly journals In silico genome-wide identification and phylogenetic analysis of the WRKY transcription factor family in sweet orange (Citrus sinensis)

2017 ◽  
Vol 11 (06) ◽  
pp. 716-726 ◽  
Author(s):  
Eduardo Goiano da Silva ◽  
◽  
Tania Mayumi Ito ◽  
Silvia Graciele Hülse de Souza ◽  
◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Transcription Factor ◽  
In Silico ◽  
Citrus Sinensis ◽  
Sweet Orange ◽  
Wrky Transcription Factor ◽  
Transcription Factor Family ◽  
Genome Wide

scholarly journals The transcription factor OpWRKY2 positively regulates the biosynthesis of the anticancer drug camptothecin in Ophiorrhiza pumila

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Xiaolong Hao ◽  
Chenhong Xie ◽  
Qingyan Ruan ◽  
Xichen Zhang ◽  
Chao Wu ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Anticancer Activity ◽  
Time Course ◽  
Indole Alkaloid ◽  
Fold Increase ◽  
Wrky Transcription Factor ◽  
Mobility Shift ◽  
Pathway Gene ◽  
Low Concentrations ◽  
Encoding Gene

AbstractThe limited bioavailability of plant-derived natural products with anticancer activity poses major challenges to the pharmaceutical industry. An example of this is camptothecin, a monoterpene indole alkaloid with potent anticancer activity that is extracted at very low concentrations from woody plants. Recently, camptothecin biosynthesis has been shown to become biotechnologically amenable in hairy-root systems of the natural producer Ophiorrhiza pumila. Here, time-course expression and metabolite analyses were performed to identify novel transcriptional regulators of camptothecin biosynthesis in O. pumila. It is shown here that camptothecin production increased over cultivation time and that the expression pattern of the WRKY transcription factor encoding gene OpWRKY2 is closely correlated with camptothecin accumulation. Overexpression of OpWRKY2 led to a more than three-fold increase in camptothecin levels. Accordingly, silencing of OpWRKY2 correlated with decreased camptothecin levels in the plant. Further detailed molecular characterization by electrophoretic mobility shift, yeast one-hybrid and dual-luciferase assays showed that OpWRKY2 directly binds and activates the central camptothecin pathway gene OpTDC. Taken together, the results of this study demonstrate that OpWRKY2 acts as a direct positive regulator of camptothecin biosynthesis. As such, a feasible strategy for the over-accumulation of camptothecin in a biotechnologically amenable system is presented.

A cotton (Gossypium hirsutum) WRKY transcription factor (GhWRKY22) participates in regulating anther/pollen development

2019 ◽  
Vol 141 ◽  
pp. 231-239 ◽  
Author(s):  
Yao Wang ◽  
Yang Li ◽  
Shao-Ping He ◽  
Ya Gao ◽  
Na-Na Wang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Gossypium Hirsutum ◽  
Pollen Development ◽  
Wrky Transcription Factor

Weak Organic Acid Stress Triggers Hyperphosphorylation of the Yeast Zinc-Finger Transcription Factor War1 and Dampens Stress Adaptation

2010 ◽  
Vol 14 (5) ◽  
pp. 575-586 ◽  
Author(s):  
Ingrid E. Frohner ◽  
Christa Gregori ◽  
Dorothea Anrather ◽  
Elisabeth Roitinger ◽  
Christoph Schüller ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Organic Acid ◽  
Zinc Finger ◽  
Acid Stress ◽  
Stress Adaptation ◽  
Zinc Finger Transcription Factor ◽  
Weak Organic Acid
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