scholarly journals Association of transcription factor WRKY56 gene from Populus simonii × P. nigra with salt tolerance in Arabidopsis thaliana

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7291 ◽  
Author(s):  
Lei Wang ◽  
Wenjing Yao ◽  
Yao Sun ◽  
Jiying Wang ◽  
Tingbo Jiang
Keyword(s):  
Transcription Factor ◽  
Salt Stress ◽  
Abiotic Stress ◽  
Salt Tolerance ◽  
Stress Responses ◽  
Germination Rate ◽  
Wrky Transcription Factor ◽  
Biotic And Abiotic Stress ◽  
Populus Simonii ◽  
Reading Frame

The WRKY transcription factor family is one of the largest groups of transcription factor in plants, playing important roles in growth, development, and biotic and abiotic stress responses. Many WRKY genes have been cloned from a variety of plant species and their functions have been analyzed. However, the studies on WRKY transcription factors in tree species under abiotic stress are still not well characterized. To understand the effects of the WRKY gene in response to abiotic stress, mRNA abundances of 102 WRKY genes in Populus simonii × P. nigra were identified by RNA sequencing under normal and salt stress conditions. The expression of 23 WRKY genes varied remarkably, in a tissue-specific manner, under salt stress. Since the WRKY56 was one of the genes significantly induced by NaCl treatment, its cDNA fragment containing an open reading frame from P. simonii × P. nigra was then cloned and transferred into Arabidopsis using the floral dip method. Under salt stress, the transgenic Arabidopsis over-expressed the WRKY56 gene, showing an increase in fresh weight, germination rate, proline content, and peroxidase and superoxide dismutase activity, when compared with the wild type. In contrast, transgenic Arabidopsis displayed a decrease in malondialdehyde content under salt stress. Overall, these results indicated that the WRKY56 gene played an important role in regulating salt tolerance in transgenic Arabidopsis.

Overexpression of DnWRKY11 enhanced salt and drought stress tolerance of transgenic tobacco

Biologia ◽  
2014 ◽  
Vol 69 (8) ◽  
Author(s):  
Xiang-Bin Xu ◽  
Yuan-Yuan Pan ◽  
Chun-Ling Wang ◽  
Qi-Cai Ying ◽  
Hong-Miao Song ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Stress Tolerance ◽  
Transgenic Tobacco ◽  
Stress Responses ◽  
Environmental Changes ◽  
Germination Rate ◽  
Defense Responses ◽  
Biotic And Abiotic Stress ◽  
Field Environment

AbstractDendrobium seedlings showed low survival rate when they were transferred from in vitro conditions to greenhouse or field environment. One of the major reasons is their low tolerance to environmental changes. WRKY transcription factors are one of the largest families of transcriptional regulators in plants. They are involved in various biotic and abiotic stress responses. One DnWRKY11 gene was isolated from Dendrobium nobile. To explore the function of DnWRKY11 in Dendrobium defense responses to abiotic stress, it was overexpressed in tobacco. Under salt and drought stresses, the DnWRKY11 transgenic tobacco showed higher germination rate, longer root length, higher fresh weight, higher activities of catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), and lower content of malonidialdehyde (MDA) than the wild type. These results proved the important roles of DnWRKY11 in plant response to drought and salt stresses, and provided a potential gene for improving environmental stress tolerance of Dendrobium seedlings.

scholarly journals Isolation, Expression, and Promoter Analysis ofGbWRKY2: A Novel Transcription Factor Gene fromGinkgo biloba

2015 ◽  
Vol 2015 ◽  
pp. 1-17 ◽  
Author(s):  
Yong-Ling Liao ◽  
Yong-Bao Shen ◽  
Jie Chang ◽  
Wei-Wei Zhang ◽  
Shui-Yuan Cheng ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Zinc Finger ◽  
Stress Responses ◽  
Genomic Dna ◽  
Single Copy ◽  
Wrky Transcription Factor ◽  
Full Length ◽  
Flanking Sequence ◽  
Reading Frame ◽  
Full Length Cdna

WRKY transcription factor is involved in multiple life activities including plant growth and development as well as biotic and abiotic responses. We identified 28WRKYgenes from transcriptome data ofGinkgo bilobaaccording to conserved WRKY domains and zinc finger structure and selected three WRKY genes, which areGbWRKY2, GbWRKY16, andGbWRKY21, for expression pattern analysis.GbWRKY2was preferentially expressed in flowers and strongly induced by methyl jasmonate. Here, we cloned the full-length cDNA and genomic DNA ofGbWRKY2. The full-length cDNA ofGbWRKY2was 1,713 bp containing a 1,014 bp open reading frame encoding a polypeptide of 337 amino acids. TheGbWRKY2genomic DNA had one intron and two exons. The deducedGbWRKY2contained one WRKY domain and one zinc finger motif.GbWRKY2was classified into Group II WRKYs. Southern blot analysis revealed thatGbWRKY2was a single copy gene inG. biloba. Manycis-acting elements related to hormone and stress responses were identified in the 1,363 bp-length 5′-flanking sequence ofGbWRKY2, including W-box, ABRE-motif, MYBCOREs, and PYRIMIDINE-boxes, revealing the molecular mechanism of upregulated expression ofGbWRKY2by hormone and stress treatments. Further functional characterizations in transiently transformed tobacco leaves allowed us to identify the region that can be considered as the minimal promoter.

scholarly journals CRK2 enhances salt tolerance in Arabidopsis thaliana by regulating endocytosis and callose deposition in connection with PLDα1

2018 ◽  
Author(s):  
Kerri Hunter ◽  
Sachie Kimura ◽  
Anne Rokka ◽  
Cuong Tran ◽  
Masatsugu Toyota ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Subcellular Localization ◽  
Stress Responses ◽  
Negative Regulator ◽  
Biotic And Abiotic Stress ◽  
Callose Deposition ◽  
Receptor Like Kinase ◽  
Stress Dependent

AbstractHigh salinity has become an increasingly prevalent source of stress to which plants need to adapt. The receptor-like protein kinases (RLKs), including the cysteine-rich receptor-like kinase (CRK) subfamily, are a highly expanded family of transmembrane proteins in plants and are largely responsible for communication between cells and the extracellular environment. Various CRKs have been implicated in biotic and abiotic stress responses, however their functions on a cellular level remain largely uncharacterized. Here we have shown that CRK2 enhances salt tolerance at the germination stage in Arabidopsis thaliana. We identified CRK2 as a negative regulator of endocytosis, under both normal growth conditions and salt stress. We also established that functional CRK2 is required for salt-induced callose deposition. In doing so, we revealed a novel role for callose deposition, in response to increased salinity, and demonstrated its importance for salt tolerance during germination. Using fluorescently tagged proteins we observed specific changes in CRK2’s subcellular localization in response to various stress treatments. Many of CRK2’s cellular functions were dependent on phospholipase D (PLD) activity, as were the subcellular localization changes. Thus we propose that CRK2 acts downstream of PLD during salt stress to regulate endocytosis and promote callose deposition, and that CRK2 adopts specific stress-dependent subcellular localization patterns in order to carry out its functions.One sentence summaryThe receptor-like kinase CRK2 acts in connection with PLDα1 to regulate endocytosis and callose deposition at plasmodesmata, enhancing salt tolerance in Arabidopsis thaliana.

scholarly journals The WRKY Transcription Factor GmWRKY12 Confers Drought and Salt Tolerance in Soybean

2018 ◽  
Vol 19 (12) ◽  
pp. 4087 ◽  
Author(s):  
Wen-Yan Shi ◽  
Yong-Tao Du ◽  
Jian Ma ◽  
Dong-Hong Min ◽  
Long-Guo Jin ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Salt Tolerance ◽  
Stress Responses ◽  
Abiotic Stress Tolerance ◽  
Wrky Transcription Factor ◽  
Rna Seq ◽  
Soybean Seedlings ◽  
Drought And Salt Tolerance ◽  
Drought And Salt Stress ◽  
Mda Content

WRKYs are important regulators in plant development and stress responses. However, knowledge of this superfamily in soybean is limited. In this study, we characterized the drought- and salt-induced gene GmWRKY12 based on RNA-Seq and qRT-PCR. GmWRKY12, which is 714 bp in length, encoded 237 amino acids and grouped into WRKY II. The promoter region of GmWRKY12 included ABER4, MYB, MYC, GT-1, W-box and DPBF cis-elements, which possibly participate in abscisic acid (ABA), drought and salt stress responses. GmWRKY12 was minimally expressed in different tissues under normal conditions but highly expressed under drought and salt treatments. As a nucleus protein, GmWRKY12 was responsive to drought, salt, ABA and salicylic acid (SA) stresses. Using a transgenic hairy root assay, we further characterized the roles of GmWRKY12 in abiotic stress tolerance. Compared with control (Williams 82), overexpression of GmWRKY12 enhanced drought and salt tolerance, increased proline (Pro) content and decreased malondialdehyde (MDA) content under drought and salt treatment in transgenic soybean seedlings. These results may provide a basis to understand the functions of GmWRKY12 in abiotic stress responses in soybean.

scholarly journals Improved salt tolerance in transgenic tobacco by over-expression of poplar NAC13 gene

2019 ◽  
Author(s):  
Xuemei Zhang ◽  
Zihan Cheng ◽  
Kai Zhao ◽  
Renhua Li ◽  
Boru Zhou ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Transgenic Tobacco ◽  
Stress Responses ◽  
Gene Networks ◽  
Molecular Mechanisms ◽  
Physiological Characterization ◽  
Protein Functions ◽  
Forest Genetic

Background: NACs are one of the major transcription factor families in plants which play an important role in plant growth and development, as well as in adverse stress responses. Methods: In this study, we cloned a salt-inducible NAC transcription factor gene (NAC13) from a poplar variety 84K, followed by transforming it into both tobacco and Arabidopsis. Results: Stable expression analysis of 35S::NAC13-GFP fusion protein in Arabidopsis indicated that NAC13 was localized to the nucleus. We also obtained five transgenic tobacco lines. Evidence from morphological and physiological characterization and salt treatment analyses indicated that the transgenic tobacco enhanced salt tolerance, suggesting that NAC13 gene may function as a positive regulator in tobacco responses to salt stress. Furthermore, evidence from yeast two-hybrid screening demonstrated that NAC13 protein functions as a transcriptional activator, with an activation domain located in the C-terminal region. Discussion: NAC13 gene plays an important role in response to salt stress in tobacco. Future studies are needed to shed light on molecular mechanisms of gene regulation and gene networks related to NAC13 gene in response to salt stress, which will provide a valuable theoretical basis for forest genetic breeding and resistant breeding.

scholarly journals Function and Mechanism of WRKY Transcription Factors in Abiotic Stress Responses of Plants

Plants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1515
Author(s):  
Weixing Li ◽  
Siyu Pang ◽  
Zhaogeng Lu ◽  
Biao Jin
Keyword(s):  
Transcription Factor ◽  
Transcription Factors ◽  
Abiotic Stress ◽  
Stress Responses ◽  
Regulatory Mechanism ◽  
Structural Characteristics ◽  
Wrky Transcription Factor ◽  
Future Perspectives ◽  
Wrky Transcription Factors ◽  
Different Response

The WRKY gene family is a plant-specific transcription factor (TF) group, playing important roles in many different response pathways of diverse abiotic stresses (drought, saline, alkali, temperature, and ultraviolet radiation, and so forth). In recent years, many studies have explored the role and mechanism of WRKY family members from model plants to agricultural crops and other species. Abiotic stress adversely affects the growth and development of plants. Thus, a review of WRKY with stress responses is important to increase our understanding of abiotic stress responses in plants. Here, we summarize the structural characteristics and regulatory mechanism of WRKY transcription factors and their responses to abiotic stress. We also discuss current issues and future perspectives of WRKY transcription factor research.

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