Fumonisin B1 induced aggressiveness and infection mechanism of Fusarium proliferatum on banana fruit

2021 ◽  
pp. 117793
Author(s):  
Lihong Xie ◽  
Yanfei Wu ◽  
Yong Wang ◽  
Yueming Jiang ◽  
Bao Yang ◽  
...  
Keyword(s):  
Fumonisin B1 ◽  
Fusarium Proliferatum ◽  
Banana Fruit ◽  
Infection Mechanism

Fumonisin Production in Corn by Toxigenic Strains of Fusarium moniliforme and Fusarium proliferatum

1994 ◽  
Vol 57 (6) ◽  
pp. 514-521 ◽  
Author(s):  
CHARLES W. BACON ◽  
PAUL E. NELSON
Keyword(s):  
Fusarium Moniliforme ◽  
Fruits And Vegetables ◽  
Animal Feed ◽  
Fusaric Acid ◽  
Fumonisin B1 ◽  
Fusarium Proliferatum ◽  
Food Crops ◽  
Ear Rot ◽  
Corn Products ◽  
Toxigenic Strains

The fungi Fusarium moniliforme Sheldon and Fusarium proliferatum (Matsushima) Nirenberg produce a series of toxins on corn which include the fumonisins of which fumonisin B1 and B2 are considered to have cancer promoting activity. Both fungi produce similar ratios of the fumonisins B1 to B2. Other mycotoxins produced include moniliformin, fusarin C and fusaric acid. Fumonisin B1 has been shown to be responsible for most of the toxicological affects observed from ingesting corn infected by toxigenic isolates of these fungi. The distribution of the two fungi is generally similar, although F. proliferatum is isolated more frequently from sorghum than corn. They occur worldwide on other food crops, such as rice, sorghum, millet, several fruits and vegetables. Both fungi are ear rot pathogens of corn, thus, mycotoxin production occurs under field conditions, although it also may occur in storage. One or both fungi may have a frequency of occurrence of 90% or higher in corn; 90% of the F. moniliforme isolates produce the fumonisins. On corn and corn products the range of concentrations reported is 0.3 to 330 μg/g of corn-based product. These concentrations include both corn-based animal feed and human foods.

Fumonisin B1 Production and Growth of Fusarium moniliforme and Fusarium proliferatum on Maize, Wheat, and Barley Grain

1999 ◽  
Vol 64 (5) ◽  
pp. 921-924 ◽  
Author(s):  
S. Marin ◽  
N. Magan ◽  
J. Serra ◽  
A.J. Ramos ◽  
R. Canela ◽  
...  
Keyword(s):  
Fusarium Moniliforme ◽  
Fumonisin B1 ◽  
Barley Grain ◽  
Fusarium Proliferatum

scholarly journals Serohematologic Alterations in Broiler Chicks on Feed Amended with Fusarium Proliferatum Culture Material or Fumonisin B1 and Moniliformin

1995 ◽  
Vol 7 (4) ◽  
pp. 520-526 ◽  
Author(s):  
Tariq Javed ◽  
Mary A. Dombrink-Kurtzman ◽  
John L. Richard ◽  
Glenn A. Bennett ◽  
L. Marie Côté ◽  
...  
Keyword(s):  
Blood Cell ◽  
Fumonisin B1 ◽  
Fusarium Proliferatum ◽  
Total Serum ◽  
Broiler Chicks ◽  
Cell Counts ◽  
Blood Cell Counts ◽  
Culture Material ◽  
Disease Antibody ◽  
Glutamyl Transferase

Two hundred twenty-eight male broiler chicks (Columbia x New Hampshire) were given feed amended with autoclaved culture material of Fusarium proliferatum containing fumonisin B1 (FB1) at 61, 193, and 546 ppm, fumonisin B2 (FB2) at 14, 38, and 98 ppm, and moniliformin at 66, 193, and 367 ppm in 3 separate feeding trials (amounts of toxin in each trial, respectively). Birds were started on amended rations at days 1, 7, and 21 and continued on their respective ration until they were 28 days old. Purified FB1 (125, 274 ppm) and moniliformin (27, 154 ppm) were given separately and in combination (137 and 77 ppm, respectively), starting on day 1 and continuing for 14 days. Of serum chemistry parameters, only glucose was significantly decreased. Significant increases were noted in serum cholesterol, sodium, alkaline phosphatase, aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, and γ-glutamyl transferase. Of the hematologic parameters, significant decreases were noted in red blood cell counts, hemoglobin, packed cell volume, and white blood cell counts. Immunologic changes included impaired anti-Newcastle disease antibody hemagglutination inhibition titers associated with relative decreases in total serum globulins and increases in albumin/globulin ratios. The changes were noted in all treatment groups when compared to controls.

scholarly journals Mycorrhizal Root Exudates Induce Changes in the Growth and Fumonisin Gene (FUM1) Expression of Fusarium proliferatum

Agronomy ◽  
2019 ◽  
Vol 9 (6) ◽  
pp. 291
Author(s):  
Zoltán Mayer ◽  
Ákos Juhász ◽  
Katalin Posta
Keyword(s):  
Root Exudates ◽  
Biological Control Agent ◽  
Fumonisin B1 ◽  
Nutrient Supply ◽  
Fusarium Proliferatum ◽  
Mitogen Activated Protein Kinase ◽  
Fungal Colonization ◽  
Kinase Gene ◽  
Mycorrhizal Root

In this study, root exudates from mycorrhizal and non-mycorrhizal plants growing at low or high nutrient supply were used in vitro to examine their effects on the growth and fumonisin B1 gene (FUM1) expression of Fusarium proliferatum (Hypocreales: Nectriaceae). After one day of exposure to root exudates originating from non-mycorrhizal and low nutrient supply treatment, a significant change in the growth of F. proliferatum was measured, which then equalized after 5 days of incubation. Aside from the fumonisin gene (FUM1) gene, the expression of the mitogen-activated protein kinase gene (HOG1) was also studied using quantitative real-time polymerase chain reaction (qRT-PCR). After 5 days of incubation, mycorrhizal root exudates significantly reduced the expression of the FUM1 gene, irrespective of the extent of the nutrient supplement and colonization level of the target plant. Similar trends in the expressions of FUM1 and HOG1 genes found in our experiment suggest that arbuscular mycorrhizal fungal colonization did not only affect directly the growth and mycotoxin production of F. proliferatum, but also modulated indirectly a number of other mechanisms. Mycorrhizal inoculation showed potential as a biological control agent in the suppression of fumonisin production by F. proliferatum.

Comparative Detection of Fumonisin by HPLC, ELISA, and Immunocytochemical Localization in Fusarium Cultures

1995 ◽  
Vol 58 (6) ◽  
pp. 666-672 ◽  
Author(s):  
MARIA VICTORIA TEJADA-SIMON ◽  
LILIAN T. MAROVATSANGA ◽  
JAMES J. PESTKA
Keyword(s):  
Monoclonal Antibody ◽  
Fusarium Moniliforme ◽  
High Performance ◽  
Time Course ◽  
Fumonisin B1 ◽  
Fusarium Proliferatum ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunocytochemical Localization ◽  
Large Deposits

Fumonisins are a group of mycotoxins that are elaborated by Fusarium moniliforme and Fusarium proliferatum and that have recently been associated with animal and human disease. In this study, the time course of fumonisin B1 (FB1) production in corn was monitored in five Fusarium cultures using high-performance liquid chromatography (HPLC), enzyme-linked immunosorbent assay (ELISA), and in situ localization by an enzyme-linked immunocytochemical technique (ELICT). Using HPLC on culture extracts prepared with 50% (vol/vol) acetonitrile in water, FB1 was detectable at 3 days with maximal FB1 (ranging from 230 to 3,000 ppm) occurring between 14 and 28 days. Although there was a positive correlation between FB1 detected by HPLC and ELISA, the latter consistently yielded higher results than HPLC. Maximal FB1 “equivalents” detected by ELISA ranged from 12,000 to 35,000 ppm. Following fixation of Fusarium from cultures, ELICT revealed the presence of large deposits indicative of fumonisin or fumonisin-like cross-reacting compounds in mycelia, microconidia, and microconidia. Prior to fixation, these compounds were extractable in 50% (vol/vol) acetonitrile in water. ELICT results qualitatively correlated with HPLC and ELISA over the time course of the cultures. Taken together, the results suggest that (a) ELISA or ELICT could be used for qualitative screening of FB1-producing cultures, and (b) in addition to FB1, the monoclonal antibody-based ELISA detected one or more compounds that structurally resemble FB1 and occur concurrently with FB1.

scholarly journals Effects of Different Carbon Sources on Fumonisin Production and FUM Gene Expression by Fusarium proliferatum

Toxins ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 289 ◽  
Author(s):  
Yu Wu ◽  
Taotao Li ◽  
Liang Gong ◽  
Yong Wang ◽  
Yueming Jiang
Keyword(s):  
Carbon Source ◽  
Biological Effect ◽  
Culture Medium ◽  
Carbon Sources ◽  
Fusarium Proliferatum ◽  
Banana Peel ◽  
Transcriptional Level ◽  
Banana Fruit ◽  
Limited Information ◽  
Future Work

Fusarium proliferatum can infect many crops and then produce fumonisins that are very harmful to humans and animals. Previous study indicates that carbon sources play important roles in regulating the fumonisin biosynthesis. Unfortunately, there is limited information on the effects of carbon starvation in comparison with the carbon sources present in the host of fumonisin production in F. proliferatum. Our results indicated that F. proliferatum cultivated in the Czapek’s broth (CB) medium in the absence of sucrose could greatly induce production of fumonisin, while an additional supplementation of sucrose to the culture medium significantly reduced the fumonisin production. Furthermore, cellulose and hemicellulose, and polysaccharide extracted from banana peel, which replaced sucrose as the carbon source, can reduce the production of fumonisin by F. proliferatum. Further work showed that these genes related to the synthesis of fumonisin, such as FUM1 and FUM8, were significantly up-regulated in the culture medium in the absence of sucrose. Consistent with fumonisin production, the expressions of FUM gene cluster and ZFR1 gene decreased after the addition of sucrose. Moreover, these genes were also significantly down-regulated in the presence of cellulose, hemicellulose or polysaccharide extracted from peel. Altogether, our results suggested that fumonisin production was regulated in F. proliferatum in response to different carbon source conditions, and this regulation might be mainly via the transcriptional level. Future work on these expressions of the fumonisin biosynthesis-related genes is needed to further clarify the response under different carbon conditions during the infection of F. proliferatum on banana fruit hosts. The findings in this study will provide a new clue regarding the biological effect of the fumonisin production in response to environmental stress.

Experimental infection of Fusarium proliferatum in Oryza sativa plants; fumonisin B1 production and survival rate in grains

2012 ◽  
Vol 156 (3) ◽  
pp. 204-208 ◽  
Author(s):  
Masayo Kushiro ◽  
Hatsuo Saitoh ◽  
Yoshitsugu Sugiura ◽  
Takayuki Aoki ◽  
Shin-ichi Kawamoto ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Survival Rate ◽  
Experimental Infection ◽  
Fumonisin B1 ◽  
Fusarium Proliferatum
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