The effects of intra-rectal and intra-peritoneal application of Origanum onites L. essential oil on 2,4,6-trinitrobenzenesulfonic acid-induced colitis in the rat

Essential oil of Origanum species is well known for antimicrobial activity, but only a few have been evaluated in narrow spectrum antiprotozoal assays. Herein, we assessed the antiprotozoal potential of Turkish Origanum onites L. oil and its major constituents against a panel of parasitic protozoa. The essential oil was obtained by hydrodistillation from the dried herbal parts of O. onites and analyzed by Gas Chromatography-Flame Ionization Detector (GC-FID) and Gas Chromatography coupled with Mass Spectrometry (GC-MS). The in vitro activity of the oil and its major components were evaluated against Trypanosoma brucei rhodesiense, T. cruzi, Leishmania donovani, and Plasmodium falciparum. The main component of the oil was identified as carvacrol (70.6%), followed by linalool (9.7%), p-cymene (7%), γ-terpinene (2.1%), and thymol (1.8%). The oil showed significant in vitro activity against T. b. rhodesiense (IC50 180 ng/mL), and moderate antileishmanial and antiplasmodial effects, without toxicity to mammalian cells. Carvacrol, thymol, and 10 additional abundant oil constituents were tested against the same panel; carvacrol and thymol retained the oil’s in vitro antiparasitic potency. In the T. b. brucei mouse model, thymol, but not carvacrol, extended the mean survival of animals. This study indicates the potential of the essential oil of O. onites and its constituents in the treatment of protozoal infections.

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Extraction, Chemical Composition, and Anticancer Potential of Origanum onites L. Essential Oil

Molecules ◽

10.3390/molecules24142612 ◽

2019 ◽

Vol 24 (14) ◽

pp. 2612 ◽

Cited By ~ 10

Author(s):

Katerina Spyridopoulou ◽

Eleni Fitsiou ◽

Eleni Bouloukosta ◽

Angeliki Tiptiri-Kourpeti ◽

Manolis Vamvakias ◽

...

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Cell Lines ◽

Cancer Cell ◽

Antiproliferative Activity ◽

Biological Activities ◽

Colorectal Cancer Cell Line ◽

Ht 29 ◽

Origanum Onites

Origanum species are plants rich in volatile oils that are mainly used for culinary purposes. In recent years, there has been a growing interest in the biological activities of their essential oils. Origanum onites L. is a plant mainly found in Greece, Turkey, and Sicily, whose oil is rich in carvacrol, a highly bioactive phytochemical. The aim of this study was to analyze the chemical composition of Origanum onites essential oil (OOEO), and investigate its potential anticancer effects in vitro and in vivo. GC/MS analysis identified carvacrol as OOEO’s main constituent. In vitro antiproliferative activity was assayed with the sulforhodamine B (SRB) assay against human cancer cell lines from four tumor types. HT-29, a colorectal cancer cell line, was the most sensitive to the antiproliferative activity of OOEO. Wound-healing assay and Annexin V-PI staining were employed to investigate the antimigratory and the pro-apoptotic potential of OOEO, respectively, against human (HT-29) and murine (CT26) colon cancer cells. Notably, OOEO attenuated migration and induced apoptosis-related morphological changes in both cell lines. Prophylactic oral administration of the oil in a BALB/c experimental mouse model inhibited the growth of syngeneic CT26 colon tumors. As far as we know, this is the first report on the antitumor potential of orally administered OOEO.

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Assessment of anti-angiogenic and anti-tumoral potentials of Origanum onites L. essential oil

Food and Chemical Toxicology ◽

10.1016/j.fct.2012.03.074 ◽

2012 ◽

Vol 50 (6) ◽

pp. 2002-2008 ◽

Cited By ~ 34

Author(s):

Rakibe Beklem Bostancıoğlu ◽

Mine Kürkçüoğlu ◽

Kemal Hüsnü Can Başer ◽

Ayşe Tansu Koparal

Keyword(s):

Essential Oil ◽

Origanum Onites

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Antioxidant and anticancer activity of essential oil from Origanum onites (Lamiaceae)

Toxicology Letters ◽

10.1016/j.toxlet.2010.03.459 ◽

2010 ◽

Vol 196 ◽

pp. S131-S132 ◽

Cited By ~ 1

Author(s):

A. Ozkan ◽

A. Erdogan ◽

N.G. Trak

Keyword(s):

Essential Oil ◽

Anticancer Activity ◽

Origanum Onites

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In vitro physiological effects of Origanum onites L. essential oil treatment on human origin cell lines

TURKISH JOURNAL OF BOTANY ◽

10.3906/bot-2010-40 ◽

2021 ◽

Keyword(s):

Essential Oil ◽

Cell Lines ◽

Physiological Effects ◽

Human Origin ◽

Origanum Onites

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Protective Effects of Origanum onites Essential Oil in the Methotrexate-Induced Rat Model: Role on Apoptosis and Hepatoxicity

Records of Natural Products ◽

10.25135/rnp.186.20.04.1631 ◽

2020 ◽

Vol 14 (6) ◽

pp. 395-404

Author(s):

Asli Aykac ◽

Eda Becer ◽

Dilek Özbeyli ◽

Göksel Şener ◽

Kemal Hüsnü Can Başer

Keyword(s):

Essential Oil ◽

Rat Model ◽

Protective Effects ◽

Origanum Onites

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A Multi-Parameter Evaluation of the Neuroprotective and Cognitive-Enhancing Effects of Origanum Onites L. (Turkish Oregano) Essential Oil on Scopolamine-Induced Amnestic Rats

10.21203/rs.3.rs-1148541/v1 ◽

2021 ◽

Author(s):

Asli Aykac ◽

Kerem Teralı ◽

Dilek Özbeyli ◽

Seren Ede ◽

Ömercan Albayrak ◽

...

Keyword(s):

Essential Oil ◽

Neurodegenerative Disorder ◽

Therapeutic Potential ◽

Age Groups ◽

Health Concern ◽

Albino Rats ◽

Herbaceous Plant ◽

Oregano Essential Oil ◽

Origanum Onites

Abstract Alzheimer’s disease (AD) is a neurodegenerative disorder characterized by progressive deterioration of cognitive functions (dementia) and represents a growing public health concern since the population in the age groups at risk is increasing. The latter raises an urgent need to translate research findings in the basic brain and behavioral sciences into anti-AD drugs and disease-modifying therapies. Origanum onites (L.), also called Turkish oregano, is a perennial and herbaceous plant species grown for centuries for medicinal, cosmetic and culinary purposes. This is the first study to investigate the putative neuroprotective and pro-cognitive activities of O. onites essential oil (OOEO) against scopolamine-induced amnesia of AD-type in Wistar albino rats. The results of behavioral tests revealed that OOEO administration was able to significantly alleviate learning and memory impairments induced by scopolamine in vivo. The observed effects could be attributed to inhibition of acetylcholinesterase activity, attenuation of oxidative stress and prevention of neuronal apoptosis in the hippocampus and frontal cortex of AD rats. Modulation of pro-inflammatory enzymes, including cyclooxygenase-2, inducible nitric oxide synthase and myeloperoxidase, might further contribute to the neuroprotective properties of OEOO, as predicted by our in silico models. These findings offer novel insights into the therapeutic potential of OEOO in patients with AD.

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