Chemical extraction and modification of chitin and chitosan from shrimp shells

2021 ◽  
Vol 159 ◽  
pp. 110709
Author(s):  
Majid Pakizeh ◽  
Ali Moradi ◽  
Toktam Ghassemi
Keyword(s):  
Chemical Extraction ◽  
Shrimp Shells ◽  
Chitin And Chitosan

Characterization of chitin and chitosan extracted from shrimp shells by two methods

e-Polymers ◽  
2010 ◽  
Vol 10 (1) ◽  
Author(s):  
Carmiña Gartner ◽  
Carlos Alberto Peláez ◽  
Betty Lucy López
Keyword(s):  
Low Cost ◽  
Magic Angle Spinning ◽  
Chemical Extraction ◽  
Magic Angle ◽  
X Ray Diffraction ◽  
Shrimp Shells ◽  
Before And After ◽  
Chitin Extraction ◽  
Angle Spinning ◽  
Chitin And Chitosan

AbstractShrimp shells from Penaeus Vannamei species were hydrolyzed for chitin extraction by a chemical and a papain enzymatic method. Composition of shells was analyzed and their microstructure was characterized before and after hydrolysis by microscopy. Chitin fibers arrangement in the tissue was preserved after chemical extraction, but after papain hydrolysis the tissue presented structural disarrangement indicating that papain reacts indistinctly with peptidic and N-acetyl linkages. Although chemical purification is very effective, by-products are not recoverable. Conversely, papain hydrolysis yields partially purified chitosan but permits aminoacids isolation, which is important in food industry. This method has other advantages such as low cost and easy accessibility of papain. Chitin and chitosan were characterized by thermogravimetric analysis, infrared spectrophotometry and capillary electrophoresis. Degree of N-acetylation (DA) was determined by cross-polarization magic angle spinning nuclear magnetic resonance (CPMAS 13CNMR) or potentiometry and crystallinity was measured by X ray diffraction.

Extraction and Characterization of Chitin and Chitosan Biopolymer as Wound Healing Material Using Shrimp Shells

2021 ◽  
Vol 10 (2) ◽  
pp. 69-75
Author(s):  
Mavis Ampah ◽  
Margaret Akyea Brago ◽  
Mercy Adusei Boatemaa ◽  
Paul Arthur ◽  
Maxwell Mamfe Sakyiamah
Keyword(s):  
Wound Healing ◽  
Antimicrobial Property ◽  
Industrial Applications ◽  
Chemical Extraction ◽  
Morbidity Rate ◽  
Mortality And Morbidity ◽  
Antimicrobial Studies ◽  
Shrimp Shells ◽  
Specific Temperature ◽  
Chitin And Chitosan

Wound refers to any damage or disruption to the normal anatomical structure. In our everyday pathology, wounds remains one of the demanding clinical problems with it related complications which increases mortality and morbidity rate yearly. In this study, chitin and chitosan were extracted from shrimp shells waste by chemical method, treated with an acid and an alkali which gave a yield of 12 % and 66.57 % respectively. The chemical extraction method included demineralization where the sample was treated with hydrochloric acid (HCL) under a specific temperature. The sample was then treated with sodium hydroxide (NaOH) to remove all the protein in the material at a specific temperature. To obtain the chitosan, the chitin was treated with 50% NaOH at a temperature of 1000C. E.coli proved to be more susceptible in antimicrobial studies with a value of 11.67± 0.47. The FTIR spectra gave a characteristic bands of –NH at 3430.09 cm-1, OH at 3256.32 cm-1.At 2960.75 cm-1, NH was attached to a single bond. The characteristics of produced chitosan were in accordance with the commercial standard that showed a higher percentile yield posing many properties of commercial value and greater scope of industrial applications. This study revealed that shrimp shell waste could be effectively utilized for the extraction of chitin, chitosan, and chitooligomer for industrial applications. The zone of inhibition study of E.coli shows that chitosan and COS may have a high antimicrobial property hence it usefulness in the wound healing management.

Chitin and chitosan preparation from shrimp shells using optimized enzymatic deproteinization

2012 ◽  
Vol 47 (12) ◽  
pp. 2032-2039 ◽  
Author(s):  
Islem Younes ◽  
Olfa Ghorbel-Bellaaj ◽  
Rim Nasri ◽  
Moncef Chaabouni ◽  
Marguerite Rinaudo ◽  
...  
Keyword(s):  
Shrimp Shells ◽  
Enzymatic Deproteinization ◽  
Chitin And Chitosan

Improved method for production of chitin and chitosan from shrimp shells

2020 ◽  
Vol 489 ◽  
pp. 107913 ◽  
Author(s):  
Trang Si Trung ◽  
Le Huyen Tram ◽  
Nguyen Van Tan ◽  
Nguyen Van Hoa ◽  
Nguyen Cong Minh ◽  
...  
Keyword(s):  
Improved Method ◽  
Shrimp Shells ◽  
Chitin And Chitosan

Production of high-quality chitin and chitosan from preconditioned shrimp shells

2006 ◽  
Vol 81 (7) ◽  
pp. 1113-1118 ◽  
Author(s):  
Nguyen Van Toan ◽  
Chuen-How Ng ◽  
Kyaw Nyein Aye ◽  
Trung Si Trang ◽  
Willem F. Stevens
Keyword(s):  
High Quality ◽  
Shrimp Shells ◽  
Chitin And Chitosan

scholarly journals The Potential of Insects as Alternative Sources of Chitin: An Overview on the Chemical Method of Extraction from Various Sources

2020 ◽  
Vol 21 (14) ◽  
pp. 4978
Author(s):  
Nurul Alyani Zainol Abidin ◽  
Faridah Kormin ◽  
Nurul Akhma Zainol Abidin ◽  
Nor Aini Fatihah Mohamed Anuar ◽  
Mohd Fadzelly Abu Bakar
Keyword(s):  
Limited Resource ◽  
Abundant Species ◽  
Chemical Extraction ◽  
Comprehensive Knowledge ◽  
The World ◽  
Yield Values ◽  
Initial Discovery ◽  
Alternative Sources ◽  
Chitin And Chitosan ◽  
Method Of Extraction

Chitin, being the second most abundant biopolymer after cellulose, has been gaining popularity since its initial discovery by Braconot in 1811. However, fundamental knowledge and literature on chitin and its derivatives from insects are difficult to obtain. The most common and sought-after sources of chitin are shellfish (especially crustaceans) and other aquatic invertebrates. The amount of shellfish available is obviously restricted by the amount of food waste that is allowed; hence, it is a limited resource. Therefore, insects are the best choices since, out of 1.3 million species in the world, 900,000 are insects, making them the most abundant species in the world. In this review, a total of 82 samples from shellfish—crustaceans and mollusks (n = 46), insects (n = 23), and others (n = 13)—have been collected and studied for their chemical extraction of chitin and its derivatives. The aim of this paper is to review the extraction method of chitin and chitosan for a comparison of the optimal demineralization and deproteinization processes, with a consideration of insects as alternative sources of chitin. The methods employed in this review are based on comprehensive bibliographic research. Based on previous data, the chitin and chitosan contents of insects in past studies favorably compare and compete with those of commercial chitin and chitosan—for example, 45% in Bombyx eri, 36.6% in Periostracum cicadae (cicada sloughs), and 26.2% in Chyrysomya megacephala. Therefore, according to the data reported by previous researchers, demonstrating comparable yield values to those of crustacean chitin and the great interest in insects as alternative sources, efforts towards comprehensive knowledge in this field are relevant.

scholarly journals Antimicrobial Activity of Chemically and Biologically Treated Chitosan Prepared from Black Soldier Fly (Hermetia illucens) Pupal Shell Waste

2021 ◽  
Vol 9 (12) ◽  
pp. 2417
Author(s):  
Mevin Kiprotich Lagat ◽  
Samuel Were ◽  
Francis Ndwigah ◽  
Violah Jepkogei Kemboi ◽  
Carolyne Kipkoech ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Properties ◽  
Diffusion Method ◽  
Chemical Extraction ◽  
Black Soldier Fly ◽  
Research Attention ◽  
Pupal Exuviae ◽  
Chitin Extraction ◽  
Acid Producing Bacteria ◽  
Chitin And Chitosan

Globally, the broad-spectrum antimicrobial activity of chitin and chitosan has been widely documented. However, very little research attention has focused on chitin and chitosan extracted from black soldier fly pupal exuviae, which are abundantly present as byproducts from insect-farming enterprises. This study presents the first comparative analysis of chemical and biological extraction of chitin and chitosan from BSF pupal exuviae. The antibacterial activity of chitosan was also evaluated. For chemical extraction, demineralization and deproteinization were carried out using 1 M hydrochloric acid at 100 °C for 2 h and 1 M NaOH for 4 h at 100 °C, respectively. Biological chitin extraction was carried out by protease-producing bacteria and lactic-acid-producing bacteria for protein and mineral removal, respectively. The extracted chitin was converted to chitosan via deacetylation using 40% NaOH for 8 h at 100 °C. Chitin characterization was done using FTIR spectroscopy, while the antimicrobial properties were determined using the disc diffusion method. Chemical and biological extraction gave a chitin yield of 10.18% and 11.85%, respectively. A maximum chitosan yield of 6.58% was achieved via chemical treatment. From the FTIR results, biological and chemical chitin showed characteristic chitin peaks at 1650 and 1550 cm−1—wavenumbers corresponding to amide I stretching and amide II bending, respectively. There was significant growth inhibition for Escherichia coli, Bacillus subtilis,Pseudomonas aeruginosa,Staphylococcus aureus, and Candida albicans when subjected to 2.5 and 5% concentrations of chitosan. Our findings demonstrate that chitosan from BSF pupal exuviae could be a promising and novel therapeutic agent for drug development against resistant strains of bacteria.

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