Direct oral mucosal epithelial transplantation supplies stem cells and promotes corneal wound healing to treat refractory persistent corneal epithelial defects

2022 ◽  
pp. 108934
Author(s):  
Danni Gong ◽  
Chenxi Yan ◽  
Fei Yu ◽  
Dan Yan ◽  
Nianxuan Wu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Corneal Wound Healing ◽  
Corneal Epithelial ◽  
Corneal Wound

scholarly journals Mesenchymal Stem Cell-Derived Extracellular Vesicles for Corneal Wound Repair

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Hongyan Tao ◽  
Xiaoniao Chen ◽  
Hongmei Cao ◽  
Lingyue Zheng ◽  
Qian Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Stem Cell ◽  
Extracellular Vesicles ◽  
Wound Repair ◽  
Bioactive Molecules ◽  
Persistent Epithelial Defect ◽  
Corneal Wound Healing ◽  
Corneal Epithelial ◽  
Corneal Wound

With the immunoregulation potential, mesenchymal stem cells (MSCs) have been used for tissue regeneration by relieving inflammation in the injured tissues. When this repair process is interfered by immune disorders or pathological angiogenesis, the delays in corneal epithelial wound healing can lead to a persistent epithelial defect. Stem cell-derived extracellular vesicles (EVs), which carry abundant bioactive molecules from stem cells, have provided an alternative to regeneration therapy. In this study, we aimed to investigate if EVs from human placenta-derived MSCs (hP-MSCs) could ameliorate alkali injury of the cornea in the mouse model. 33.33 μg/μL EVs in 10 μL PBS were applied to the cornea. Repeat application three times, and 100 μg EVs (in 30 μL PBS) in total were administrated per day for two weeks. Our results revealed that EVs from hP-MSCs had preferable functions including enhancing proliferation and anti-inflammation and suppressing apoptosis of corneal epithelial cells. Furthermore, hP-MSC-derived EVs ameliorated mouse corneal wound healing by inhibiting angiogenesis and inflammation. Taken together, our current data suggested that hP-MSC-derived EVs have the beneficial effects of corneal wound healing, which provide alternative cell-free therapy with great practical value.

scholarly journals Effect of Nitric Oxide on Human Corneal Epithelial Cell Viability and Corneal Wound Healing

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Joo-Hee Park ◽  
Ja-Yeon Kim ◽  
Dong Ju Kim ◽  
Martha Kim ◽  
Minwook Chang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Wound Healing ◽  
Epithelial Cell ◽  
Cell Viability ◽  
Corneal Epithelial Cell ◽  
Corneal Wound Healing ◽  
Corneal Epithelial ◽  
Human Corneal Epithelial Cell ◽  
Corneal Wound

A nano-phytochemical ophthalmic solution for marked improvement of corneal wound healing in healthy or diabetic mice

Nanomedicine ◽  
2021 ◽  
Author(s):  
Qiqi Li ◽  
Meng Xin ◽  
Xianggen Wu ◽  
Bo Lei
Keyword(s):  
Wound Healing ◽  
Ophthalmic Solution ◽  
Diabetic Mice ◽  
Promoting Effect ◽  
Related Factors ◽  
Corneal Wound Healing ◽  
Corneal Epithelial ◽  
Trigeminal Neurons ◽  
Corneal Wound

Aim: To formulate a novel nano-phytochemical ophthalmic solution to promote corneal wound healing. Methods: Dipotassium glycyrrhizinate (DG) and palmatine (PAL) were used to formulate this formulation marked as DG-PAL, and its efficacy and mechanisms for promoting corneal wound healing were evaluated in mice. Results: DG-PAL was easily fabricated with excellent physical profiles. In in vivo efficiency evaluations, DG-PAL demonstrated an excellent promoting effect on corneal epithelial/nerve wound healing in both healthy and diabetic mice. These effects were involved in the DG-PAL-induced decreased expression levels of HMGB1 and its signaling-related factors in the corneas and trigeminal neurons of the healthy or diabetic mice. Conclusion: DG-PAL possibly represents a promising ophthalmic solution for promoting corneal wound healing.

MiRNA-155-5p Reduces Corneal Epithelial Permeability by Remodeling Epithelial Tight Junctions during Corneal Wound Healing

2020 ◽  
Vol 45 (8) ◽  
pp. 904-913
Author(s):  
Feng Wang ◽  
Duomei Wang ◽  
Meng Song ◽  
Qing Zhou ◽  
Rongfeng Liao ◽  
...  
Keyword(s):  
Wound Healing ◽  
Tight Junctions ◽  
Epithelial Permeability ◽  
Corneal Wound Healing ◽  
Corneal Epithelial ◽  
Corneal Wound ◽  
Epithelial Tight Junctions

scholarly journals Caveolin-1 regulates corneal wound healing by modulating Kir4.1 activity

2016 ◽  
Vol 310 (11) ◽  
pp. C993-C1000 ◽  
Author(s):  
Chengbiao Zhang ◽  
Xiaotong Su ◽  
Lars Bellner ◽  
Dao-Hong Lin
Keyword(s):  
Wound Healing ◽  
Epithelial Cells ◽  
Western Blot ◽  
Negative Control ◽  
Promoting Effect ◽  
Corneal Epithelial Cells ◽  
Corneal Wound Healing ◽  
Corneal Epithelial ◽  
Caveolin 1 ◽  
Corneal Wound

The expression of caveolin-1 (Cav1) in corneal epithelium is associated with regeneration potency. We used Cav1−/− mice to study the role of Cav1 in modulating corneal wound healing. Western blot and whole cell patch clamp were employed to study the effect of Cav1 deletion on Kir4.1 current density in corneas. We found that Ba2+-sensitive K+ currents in primary cultured murine corneal epithelial cells (pMCE) from Cav1−/− were dramatically reduced (602 pA) compared with those from wild type (WT; 1,300 pA). As a consequence, membrane potential was elevated in pMCE from Cav1−/− compared with that from WT (−43 ± 7.5 vs. −58 ± 4.0 mV, respectively). Western blot showed that either inhibition of Cav1 expression or Ba2+ incubation stimulated phosphorylation of the EGFR. The transwell migration assay showed that Cav1 genetic inactivation accelerated cell migration. The regrowth efficiency of human corneal epithelial cells (HCE) transfected with siRNA-Cav1 or negative control was evaluated by scrape injury assay. With the presence of mitomycin C (10 μg/ml) to avoid the influence of cell proliferation, Cav1 inhibition with siRNA significantly increased migration compared with control siRNA in HCE. This promoting effect by siRNA-Cav1 could not be further enhanced by cotransfection with siRNA-Kcnj10. By using corneal debridement, we found that wound healing was significantly accelerated in Cav1−/− compared with WT mice (70 ± 10 vs. 36 ± 3%, P < 0.01). Our findings imply that the mechanism by which Cav-1 knockout promotes corneal regrowth is, at least partially, due to the inhibition of Kir4.1 which stimulates EGFR signaling.

scholarly journals Hyaluronate Acid-Dependent Protection and Enhanced Corneal Wound Healing against Oxidative Damage in Corneal Epithelial Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Jing Zhong ◽  
Yuqing Deng ◽  
Bishan Tian ◽  
Bowen Wang ◽  
Yifang Sun ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Epithelial Cells ◽  
Cell Apoptosis ◽  
Corneal Epithelial Cell ◽  
Corneal Epithelial Cells ◽  
Corneal Wound Healing ◽  
Corneal Epithelial ◽  
Expression Levels ◽  
Corneal Wound

Purpose. To evaluate the effects and mechanism of exogenous hyaluronate (HA) in promoting corneal wound healing.Methods. Human corneal epithelial cells (HCECs) were incubated with different concentrations of HA to evaluate their efficiency in promoting cell migration and their modulation of repair factors. After inducing hyperosmolar conditions, the cell morphologies, cell apoptosis, and expression levels of TNF-αand MMP-9 were detected to assess the protective role of HA. Corneal epithelium-injured rat models were established to test the therapeutic effects of 0.3% HA. Then, the wound healing rates, the RNA expression levels of inflammatory cytokines, and repair factors were examined.Results. HCECs in the 0.03% and 0.3% HA groups showed fewer morphological alterations and lower rates of cell apoptosis following preincubation with HA under hyperosmolar conditions, as well as the expression levels of MMP-9 and TNF-α. In the rat model, the areas of fluorescein staining in the corneas of 0.3% HA group were significantly smaller than the control group. The expression levels of IL-1βand MMP-9 were decreased, while CD44 and FN were increased in the 0.3% HA group.Conclusion. HA enhanced corneal epithelial cell wound healing by promoting cell migration, upregulating repair responses, and suppressing inflammatory responses.

scholarly journals Potential role of corneal epithelial cell-derived exosomes in corneal wound healing and neovascularization

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Kyu-Yeon Han ◽  
Jennifer A. Tran ◽  
Jin-Hong Chang ◽  
Dimitri T. Azar ◽  
James D. Zieske
Keyword(s):  
Wound Healing ◽  
Epithelial Cell ◽  
Potential Role ◽  
Corneal Epithelial Cell ◽  
Corneal Wound Healing ◽  
Corneal Epithelial ◽  
Corneal Wound

scholarly journals Novel nanopolymer RNA therapeutics normalize human diabetic corneal wound healing and epithelial stem cells

2021 ◽  
Vol 32 ◽  
pp. 102332
Author(s):  
Andrei A. Kramerov ◽  
Ruchi Shah ◽  
Hui Ding ◽  
Eggehard Holler ◽  
Sue Turjman ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Epithelial Stem Cells ◽  
Corneal Wound Healing ◽  
Corneal Wound

Therapeutic Proteins for Treatment of Corneal Epithelial Defects

2019 ◽  
Vol 26 (3) ◽  
pp. 517-545 ◽  
Author(s):  
Evgeni Yu. Zernii ◽  
Viktoriia E. Baksheeva ◽  
Elena V. Yani ◽  
Pavel P. Philippov ◽  
Ivan I. Senin
Keyword(s):  
Wound Healing ◽  
De Novo ◽  
Pain Syndrome ◽  
Active Components ◽  
Preclinical Development ◽  
Corneal Wound Healing ◽  
Corneal Epithelial ◽  
Corneal Scarring ◽  
Corneal Wound ◽  
Systemic Factors

Corneal epithelial disorders take pride of place in modern ophthalmology. Defects of corneal epithelium are commonly accompanied by blurry vision, photophobia and tearing. Since cornea is the most densely innervated tissue of organisms, its disruption leads to development of a severe pain syndrome. Mild corneal erosions commonly undergo quick spontaneous recovery. Suppression of corneal wound healing due to various pathological causes results in development of severe recurrent erosions and persistent corneal defects. These pathological events can in turn lead to corneal scarring, opacification, and ulceration of cornea, and ultimately to the permanent vision impairment. The etiology of the underlying corneal diseases that commonly involves inflammatory, neurotrophic and systemic factors, should be considered for treating such defects. Therefore, the research focus has been shifted to establish therapeutics based on proteins and peptides. Due to varied mechanisms of action, proteinbased pharmaceuticals can be involved in the protection of corneal surface, mimicking tear components, stimulation of corneal wound healing, regeneration of corneal innervation, suppressing oxidative stress, inflammation and neovascularization. The active components can be naturally occurring (blood- or tear-derived) or be created de novo and optimized in order to achieve the level of activity required. Such pharmaceuticals are characterized by low toxicity and absence of systemic side-effects due to their low absorption into the bloodstream, if administrated topically. This review summarizes existing data on protein-based drugs for treatment of corneal epithelial defects that are currently under preclinical development or testing in clinical trials, or approved for medical use.

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