Ultrasound pretreated rice bran for Rhizopus sp. phytase production as a feed

2021 ◽  
pp. 101281
Author(s):  
Subramaniyam Suresh ◽  
Ramachandran Sivaramakrishnan ◽  
Kuravappullam Vedhaiyan Radha ◽  
Aran Incharoensakdi ◽  
Arivalagan Pugazhendhi
Keyword(s):  
Rice Bran ◽  
Phytase Production ◽  
Rhizopus Sp

scholarly journals Sperathe effects of solid-state fermentation in the functional properties of defatted rice bran and wheat bran

2009 ◽  
Vol 52 (6) ◽  
pp. 1555-1562 ◽  
Author(s):  
Cristina Moreira da Silveira ◽  
Eliana Badiale-Furlong
Keyword(s):  
Solid State ◽  
Protein Content ◽  
Functional Properties ◽  
Rice Bran ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Protein Solubility ◽  
Defatted Rice Bran ◽  
Rhizopus Sp ◽  
Water Holding

Functional properties of fermented bran produced by Aspergillus oryzae and Rhizopus sp. in a solid-state fermentation system were determined, with an aim to evaluate their application in food formulation. The defatted rice bran and wheat bran were inoculated with the spores of the cultures and incubated at 30º C for 72 h. Samples were withdrawn at 0, 24, 48 and 72 h. Protein content, protein solubility, in-vitro digestibility, gelation and water holding capacity were determined in bran with or without fermentation. Rhizopus sp. increased significantly the protein content (69.0 and 56.0%, respectively, for defatted rice bran and wheat bran); protein solubility (28.5 and 36.2) and water holding capacity (11.4% for wheat bran). When A. oryzae was used all these properties were modified significantly after fermentation.

scholarly journals Rice bran as a substrate for proteolytic enzyme production

2006 ◽  
Vol 49 (5) ◽  
pp. 843-851 ◽  
Author(s):  
Alagarsamy Sumantha ◽  
Paul Deepa ◽  
Chandran Sandhya ◽  
George Szakacs ◽  
Carlos Ricardo Soccol ◽  
...  
Keyword(s):  
Rice Bran ◽  
Enzyme Production ◽  
Proteolytic Enzyme ◽  
Initial Moisture Content ◽  
Fold Increase ◽  
Maximal Activity ◽  
Protease Production ◽  
Rhizopus Microsporus ◽  
Rhizopus Sp ◽  
Optimized Conditions

Rice bran was used as the substrate for screening nine strains of Rhizopus sp. for neutral protease production by solid-state fermentation. The best producer, Rhizopus microsporus NRRL 3671, was used for optimizing the process parameters for enzyme production. Fermentation carried out with 44.44 % initial moisture content at a temperature of 30 C for 72 h was found to be the optimum for enzyme secretion by the fermenting organism. While most of the carbon supplements favored enzyme production, addition of casein resulted in a marginal increase in protease yield. Fermentation was then carried out under optimized conditions to obtain the crude extract of the enzyme, which was partially purified by precipitation and dialysis. A 3-fold increase in the enzyme purity was achieved in this manner. The enzyme was found to be a metalloprotease, being activated by Mn2+, with maximal activity at a temperature of 60 C and pH 7.0.

scholarly journals Optimization of cultivation conditions for the production of phytate-degrading enzymes by Enterobacter sakazakii ASUIA279 isolated from Malaysian maize root

2012 ◽  
Vol 3 (2) ◽  
pp. 1-10
Author(s):  
Anis Shobirin Meor Hussin ◽  
Abd El Aziem Farouk ◽  
Ralf Greiner
Keyword(s):  
Rice Bran ◽  
Incubation Temperature ◽  
Enterobacter Sakazakii ◽  
Cultivation Conditions ◽  
Phytase Production ◽  
Initial Ph ◽  
The Media ◽  
Linear Effects ◽  
Full Factorial ◽  
Agitation And Aeration

The production of extracellular phytase by Enterobacter sakazakii ASUIA279 was optimized using response surface methodology with full-factorial faced centred central composite design. Two sets of experiments were carried out to optimize the five most profound factors of the cultivation conditions in order to maximize phytase production. Incubation temperature, initial pH of the media and percentage of rice bran supplemented into the media were optimized in Erlenmeyer flasks while agitation and aeration effect were controlled in a bioreactor. This design reduced the number of actual experiment performed to optimize phytase production and allowed the study of possible interactions among the factors. In the first set of experiments linear and quadratic effect of initial pH was determined to be the most significant factor affecting phytase production. In the bioreactor both linear effects of agitation and aeration, were identified to be highly significant (> 99 %) in respect to phytase yields. Optimal phytase production was observed at a incubation temperature of 39.7 ºC, an initial pH of 7.1, supplementation with 13.6 % rice bran , 320 rpm of agitation and 0 vvm of aeration.  

Purification and characterization of phytase from Bacillus lehensis MLB2

Biologia ◽  
2015 ◽  
Vol 70 (3) ◽  
Author(s):  
Sunil S. More ◽  
Supreeth Shrinivas ◽  
Anushree Agarwal ◽  
Arpitha Chikkanna ◽  
Bhavya Janardhan ◽  
...  
Keyword(s):  
Rice Bran ◽  
Sodium Dodecyl ◽  
Cost Effective ◽  
Potassium Nitrate ◽  
Phytase Production ◽  
Inoculum Level ◽  
Maximum Stability ◽  
Bacterium Bacillus ◽  
Acidic Region

AbstractA potent phytase-producing bacterium Bacillus lehensis MLB2 was isolated from bean-grown soil. The optimum conditions recorded after optimization were 24 h incubation time, pH 5.5, 37°C, 2% inoculum level, 0.5% rice bran and 0.5% potassium nitrate. An overall 3.144-fold enhancement in phytase production was achieved after optimization. The use of an inexpensive substrate rice bran and short incubation period make the phytase production cost effective. The purified phytase (152.9 U/mg) had a molecular mass of approximately 98.686 kDa as determined by sodium dodecyl sulphatepolyacryalamide gel electrophoresis and confirmed by liquid chromatography-mass spectrometry, optimum pH of 4.5, and temperature of 37°C. It maintained maximum stability in the acidic region from pH 2.0 to 6.0 and retained 100% at 60◦C or below. It showed an enhanced activity in the presence of 5 mM K

Fermented rice bran extract improves dyslipidemia in rodents

2018 ◽  
Vol 48 (2) ◽  
pp. 375-383 ◽  
Author(s):  
Bira Arumndari Nurrahma ◽  
Mega Febia Suryajayanti ◽  
Anggi Laksmita Dewi ◽  
Zunamilla Khairia ◽  
Rio Jati Kusuma ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Lipid Profile ◽  
Rice Bran ◽  
Blood Lipid ◽  
Control Group ◽  
Sprague Dawley ◽  
Content Type ◽  
Rice Bran Extract ◽  
Glucose Levels ◽  
Rhizopus Sp

Purpose The study aims to investigate the potency of fermented rice bran extract as anti-hypercholesterolemia product by looking at its effect on lipid profile levels and blood glucose levels in dyslipidemia model rats. Design/methodology/approach Rice bran was fermented using Rhizopus oligosporus-contained tempeh mold extracted using distilled water. Twenty-four Sprague Dawley rats were divided into a control group and hypercholesterolemia groups. Hypercholesterolemia, also known as dyslipidemia, was induced with fructose-supplemented high-fat diet. Rats induced with dyslipidemia received three different fermented rice bran extract doses, 0 (negative) 1102.5 mg/kgBW/day (FRBE 1) and 2205 mg/kgBW/day (FRBE 2). Blood was collected before and after four weeks of treatment for lipid profile and blood glucose analysis. Findings FRBE 2 had significantly lower total cholesterol (101.6 ± 3.3 vs 187.6 ± 3.7 mg/dL), triglyceride (83.3 ± 2.8 vs 130.7 ± 3.4 mg/dL) and LDL level (27.9 ± 1.7 vs 76.7 ± 1.5 mg/dL) but higher HDL level (64.1 ± 3.0 vs 25.5 ± 1.2 mg/dL) compared to the negative group (p < 0.001). Provision of fermented rice bran showed dose-response relationship in all blood lipid markers. Originality/value This study was the first to investigate the effectivity of Rhizopus sp.-fermented rice bran extract to improve glucose and lipid profile.

scholarly journals Pengaruh Variasi Media terhadap Aktivitas Fitase Burkholderia Sp. Strain HF. 7

bionature ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Hafsan Hafsan ◽  
Muhammad Maslan ◽  
Mashuri Masri ◽  
Laily Agustina ◽  
Asmuddin Natsir ◽  
...  
Keyword(s):  
Phytic Acid ◽  
Rice Bran ◽  
Growth Phase ◽  
Animal Feed ◽  
Phytase Activity ◽  
Cereal Crops ◽  
Phytase Production ◽  
Descriptive Approach ◽  
Current Value ◽  
Low Activity

Abstract.  Cereals and legumes used as animal feed containing phytic acid which cannot be absorbed by the digestive tract of monogastric animals because phytic acid (C6H18O24P6) is an antinutrient that binds approximately 80% P in feed, also binds to proteins, vitamins and minerals (Mg++, Fe++, Zn++, Mn++, Ca++). Then, one option to overcome this problem is the application of phytase enzymes from various sources, including those produced by bacteria. Phytase enzymes can hydrolyse phytic acid in the feed. This study was aimed to determine the growth phase of Burkholderia sp. HF.7 and optimisation of production of phytase activity from variations of Phytate Production Media (PPM) media. This research used as a descriptive approach. The study design used a completely randomised design (CRD) with a factorial pattern consisting of 2 factors, each variation in phytate sources: calcium (Ca) phytate, rice bran, corn bran, and soybeans. Nitrogen source: (NH4) 2SO4, yeast extract, and peptone. Burkholderia sp. HF.7 Growth phase as a standard for phytase production is the 62-hour stationary phase with an OD value of 2.060 log/cell. The optimum phytase production in the variation of PPM media is soybean-peptone with a protein content value of 46.5 mg/mL and an activity value of 8.20 U/mL under conditions of pH 7 with incubation of 37oC for 62 hours. So, phytase activity produced by PPM of cereal crops has a higher current value compared to PPM Ca-phytate media with low activity value.Keywords: Burkholderia, Phytate, Phytase, enzymes.

Anti-atherogenic and Nephroprotective Effects of Tocotrienol Rich Fraction (TRF) from Palm Oil and Rice Bran Oil

Planta Medica ◽  
2010 ◽  
Vol 76 (05) ◽  
Author(s):  
MR Khan ◽  
S Siddiqui ◽  
K Parveen ◽  
WA Siddiqui
Keyword(s):  
Rice Bran ◽  
Palm Oil ◽  
Rice Bran Oil ◽  
Tocotrienol Rich Fraction

The role of rice bran extract on acetyl CoA carboxylase in liver of rats fed a high-fat diet

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
C Charkhonpunya ◽  
S Sireeratawong ◽  
S Komindr ◽  
N Lerdvuthisopon
Keyword(s):  
Rice Bran ◽  
High Fat Diet ◽  
Acetyl Coa Carboxylase ◽  
High Fat ◽  
Acetyl Coa ◽  
Rice Bran Extract

Developing a nutraceutical from Egyptian stabilized rice bran: a pharmacological approach

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
AM Helal ◽  
MT Khayyal ◽  
HM Abd El Aziz ◽  
RM Abdel Salam
Keyword(s):  
Rice Bran ◽  
Pharmacological Approach
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