Efficiency of the optimized microwave assisted extractions on the yield, chemical composition and biological activities of Tunisian Rosmarinus officinalis L. essential oil

2017 ◽  
Vol 105 ◽  
pp. 224-233 ◽  
Author(s):  
Asma Farhat ◽  
Hasnia Benmoussa ◽  
Rafik Bachoual ◽  
Zina Nasfi ◽  
Walid Elfalleh ◽  
...  

2019 ◽  
Vol 2019 ◽  
pp. 1-6 ◽  
Author(s):  
Majda Elyemni ◽  
Bouchra Louaste ◽  
Imane Nechad ◽  
Taha Elkamli ◽  
Abdelhak Bouia ◽  
...  

The extraction of essential oils is generally carried out by two main techniques: azeotropic distillation (hydrodistillation, hydrodiffusion, and steam distillation) and extraction with solvents. However, these traditional methods are a bit expensive, especially since they are extremely energy and solvent consuming. This work consists in studying two methods of extraction of the essential oils of Rosmarinus officinalis L.: microwave assisted hydrodistillation (MAH) and Clevenger hydrodistillation (CH). Several parameters have been studied: the extraction time, the yield, and the chemical composition of the essential oils as well as the efficiency and cost of each procedure. The results obtained revealed that microwave-assisted hydrodistillation makes it possible to minimize the extraction time of the essential oils in comparison with conventional hydrodistillation. Thus, the same yield of essential oils is obtained for 20 minutes only with MAH while it takes 180 minutes with CH. In addition, the quality of the essential oil is improved thanks to a 1.14% increase in oxygenates. In conclusion, the MAH method offers significant advantages over conventional hydrodistillation and can therefore replace it on a pilot and industrial scale.



2005 ◽  
Vol 68 (4) ◽  
pp. 790-795 ◽  
Author(s):  
S. SANTOYO ◽  
S. CAVERO ◽  
L. JAIME ◽  
E. IBAÑEZ ◽  
F. J. SEÑORÁNS ◽  
...  

The chemical composition and antimicrobial activity of essential oil–rich fractions obtained by supercritical CO2 extraction from Rosmarinus officinalis L. were investigated. Gas chromatography–mass spectroscopy analysis of these fractions resulted in the identification of 33 compounds of the essential oil. The main components of these fractions were α-pinene, 1,8-cineole, camphor, verbenone, and borneol, constituting ca. 80% of the total oil. The antimicrobial activity was investigated by the disc diffusion and broth dilution methods against six microbial species, including gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis), gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa), a yeast (Candida albicans), and a fungus (Aspergillus niger). All of the essential oil–rich fractions obtained showed antimicrobial activity against all of the microorganisms tested, with inhibition zones and minimal bactericidal and fungicidal concentration values in the range of 17 to 33 mm and 2.25 to 0.25 mg/ml, respectively. The most active fraction was the one obtained in experiment 4 (4% ethanol as modifier; extraction pressure, 25 MPa; extraction temperature, 60°C). S. aureus was found to be the most sensitive bacteria to the rosemary extracts, whereas the least susceptible was A. niger. α-Pinene, 1,8-cineole, camphor, verbenone, and borneol standards also showed antimicrobial activity against all the microorganisms tested, borneol being the most effective followed by camphor and verbenone. In that way, it was confirmed that essential oil from experiment 4, with the best antimicrobial activity, presented the highest quantity of camphor, borneol, and verbenone.



2013 ◽  
Vol 25 (5) ◽  
pp. 2601-2603 ◽  
Author(s):  
K. Hcini ◽  
J.A. Sotomayor ◽  
M.J. Jordan ◽  
S. Bouzid




2020 ◽  
Vol 10 ◽  
Author(s):  
Rizwana Khatoon ◽  
Md. Aftab Alam ◽  
Pramod Kumar Sharma

Aim: The purpose of this review paper is to elaborate many nano-formulations of Rosemary which are used against many diseases and also explain the Pharmacological activity of phytocompounds of Rosemary and details about the applications and uses of Rosemary in many fields. Objectives: Rosemary (Rosmarinus officinalis L.) is a medicinal herbal plant, their leaves are used in Europe, America, and Asia as a food additive. It is very valuable, their parts contain many chemical constituents which are very effective against many diseases like diabetes, obesity, cancer, infections and also have antioxidant, anti-inflammation, memory-improvement, etc. properties. Results: In the literature reviewed, Rosmarinus officinalis (rosemary) has shown exciting potential both as a natural food preservative and as a therapeutic agent. Rosemary (Rosmarinus officinalis) essential oil has been extensively studied due to its chemical composition and biological activities. The extract of Rosemary also shows the medicinal properties like anticancer, antimicrobial, antidiabetic, anti-inflammatory etc. From many studies, it was also found that nano-formulation of Rosemary had been developed by using the Rosemary essential oil and its extract which are used in the diagnosis and treatment of many diseases. Conclusion: From the conventional times, therapeutic effects of Rosemary are seen, which is also used in Modern Medicine now days. It had been used as food additives in the conventional times. In renal colic and dysmenorrhea, it is used as an antispasmodic to relieve respiratory disorders and to boost hair development. CNS, cardiac and locomotive activity promotes inhalation and oral administration of Rosemary oil, suggesting the direct effect of one or more of its constituents. Rosemary have curative potential in the therapy and avoidance of bronchial asthma, peptic ulcer, spasmodic disorders, inflammatory illnesses, atherosclerosis, hepatotoxicity, cancer, ischemic heart illness, bad sperm motility, Alzheimer, and may act as antidepressant and anxiolytic properties.



Foods ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1768
Author(s):  
Stefania Garzoli ◽  
Valentina Laghezza Masci ◽  
Sara Franceschi ◽  
Antonio Tiezzi ◽  
Pierluigi Giacomello ◽  
...  

In this work, essential oils (EOs) and hydrolates (Hys) of Rosmarinus officinalis L. and Lavandula angustifolia Mill., grown in Tuscany (Italy), were studied to describe their chemical composition and biological activities. The aromatic profile of the EOs liquid phase was carried out by gas chromatography–mass spectrometry (GC–MS), while the volatile composition of vapor phase EOs and Hys was performed by headspace (HS)/GC–MS. The obtained results show that monoterpene hydrocarbons (71.5% and 89.5%) were the main compounds, followed by oxygenated monoterpenes (26.0% and 10.5%) in the liquid and vapor phase of R. officinalis EO, respectively. The oxygenated monoterpenes were the main components of L. angustifolia EO, reaching 86.9% in the liquid phase and 53.7% in the vapor phase. Regarding Hys, they consisted only of oxygenated monoterpenes, and 1,8-cineole (56.2%) and linalool (42.9%), were the main components of R. officinalis and L. officinalis Hys, respectively. Their cytotoxicity was investigated on an SHSY5Y neuroblastoma cell line by thiazolyl blue tetrazolium bromide (MTT) test, showing a notable effect of the EOs with a time-independent manner of activity and half maximal effective concentration (EC50) values quite similar for the two plant species (from 0.05% to 0.06% v/v for the three time points evaluated). A measurable activity of Hys was also obtained although with higher EC50 values. The antibacterial activity against Escherichia coli ATCC® 25922, Pseudomonas fluorescens ATCC® 13525, Acinetobacter bohemicus DSM 102855 as Gram-negative bacteria and Kocuria marina DSM 16420, Bacillus cereus ATCC® 10876 as Gram-positive bacteria, was evaluated by the agar disk-diffusion method and the VPT (vapor phase test) to determinate the MIC (minimal inhibitory concentration) and the MBC (minimal bactericidal concentration) values. Both EOs possessed a high activity against all the bacterial strains with MIC values ranging from 0.19% to 3.13% v/v. Unlike EOs, Hys did not show an inhibition of the bacterial growth at the tested concentrations. Furthermore, antioxidant power was measured by 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt-based (ABTS•+) and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays, showing a remarkable ability to reduce radicals by both EOs; Hys were slightly less active. The findings highlighted that R. officinalis and L. angustifolia EOs and Hys have a chemical composition rich in bioactive molecules, which can exert different biological activities.



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