Potential role of toll-like receptor 3 in a murine model of polyinosinic-polycytidylic acid-induced embryo resorption

2006 ◽  
Vol 85 ◽  
pp. 1125-1129 ◽  
Author(s):  
Y LIN ◽  
Y ZENG ◽  
S ZENG ◽  
T WANG
Keyword(s):  
Murine Model ◽  
Potential Role ◽  
Toll Like Receptor ◽  
Polycytidylic Acid ◽  
Embryo Resorption ◽  
Polyinosinic Polycytidylic Acid

scholarly journals The Imbalance between Foxp3+Tregs and Th1/Th17/Th22 Cells in Patients with Newly Diagnosed Autoimmune Hepatitis

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Ma Liang ◽  
Zhang Liwen ◽  
Zhuang Yun ◽  
Ding Yanbo ◽  
Chen Jianping
Keyword(s):  
Autoimmune Hepatitis ◽  
Murine Model ◽  
Potential Role ◽  
Serum Levels ◽  
Newly Diagnosed ◽  
Foxp3 Mrna ◽  
Th22 Cells ◽  
Pathogenic Process ◽  
Experimental Autoimmune

This study is aimed at examining the potential role of regulatory T- (Treg-) Th1-Th17-Th22 cells in the pathogenic process of autoimmune hepatitis (AIH). The numbers of Foxp3+Tregs and Th1, Th17, and Th22 cells were measured in 32 AIH patients using flow cytometry. Moreover, a murine model of experimental autoimmune hepatitis (EAH) was also established and used to investigate the function of Treg-Th1-Th17-Th22 cells in disease progression. AIH patients undergoing an active state had significantly decreased numbers of CD3+CD4+CD25+Foxp3+Tregs and increased numbers of CD3+CD4+CD25−Foxp3+T, CD3+CD4+IFN-γ+Th1, CD3+CD4+IL-17+Th17, and CD3+CD4+IL-2+Th22 cells as well as higher levels of Th1/Th17/Th22-type cytokines compared to AIH patients in remission and HC. Additionally, the numbers of CD3+CD4+CD25+Foxp3+Tregs were negatively correlated with the numbers of Th1-Th17-Th22 cells. Also, the serum levels of IL-17A and IL-22 were correlated positively with liver injury (ALT/AST), whereas the serum levels of IL-10 were correlated negatively with hypergammaglobulinaemia (IgG, IgM) in AIH patients. Interestingly, the percentages of spleen Tregs, expression of Foxp3 mRNA, and liver IL-10 levels decreased, whereas the percentages of spleen Th1-Th17-Th22 cells, expression of T-bet/AHR/RORγt mRNA, and liver IFN-γ, IL-17, and IL-22 levels increased in the murine model of EAH. Our findings demonstrated that an imbalance between Tregs and Th1-Th17-Th22 cells might contribute to the pathogenic process of AIH.

scholarly journals Length-dependent recognition of double-stranded ribonucleic acids by retinoic acid–inducible gene-I and melanoma differentiation–associated gene 5

2008 ◽  
Vol 205 (7) ◽  
pp. 1601-1610 ◽  
Author(s):  
Hiroki Kato ◽  
Osamu Takeuchi ◽  
Eriko Mikamo-Satoh ◽  
Reiko Hirai ◽  
Tomoji Kawai ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Rna Recognition ◽  
Rna Helicases ◽  
Ribonucleic Acids ◽  
Polycytidylic Acid ◽  
Differential Recognition ◽  
Vesicular Stomatitis ◽  
Polyinosinic Polycytidylic Acid ◽  
Inducible Gene

The ribonucleic acid (RNA) helicases retinoic acid-inducible gene-I (RIG-I) and melanoma differentiation–associated gene 5 (MDA5) recognize distinct viral and synthetic RNAs, leading to the production of interferons. Although 5′-triphosphate single-stranded RNA is a RIG-I ligand, the role of RIG-I and MDA5 in double-stranded (ds) RNA recognition remains to be characterized. In this study, we show that the length of dsRNA is important for differential recognition by RIG-I and MDA5. The MDA5 ligand, polyinosinic-polycytidylic acid, was converted to a RIG-I ligand after shortening of the dsRNA length. In addition, viral dsRNAs differentially activated RIG-I and MDA5, depending on their length. Vesicular stomatitis virus infection generated dsRNA, which is responsible for RIG-I–mediated recognition. Collectively, RIG-I detects dsRNAs without a 5′-triphosphate end, and RIG-I and MDA5 selectively recognize short and long dsRNAs, respectively.

247 The Role of Toll-Like Receptor-4 in Gut-Brain Cross Talk in a Murine Model of Parkinson's Disease

2014 ◽  
Vol 146 (5) ◽  
pp. S-59
Author(s):  
Paula Perez-Pardo ◽  
Hidde Douna ◽  
Tom Wijnands ◽  
Sofia Lopes da Silva ◽  
Christopher B. Forsyth ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Cross Talk ◽  
Murine Model ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4
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