The effect of different synthetic serum supplementation for fertilization media on sperm capacitation in a short incubation period

2014 ◽  
Vol 102 (3) ◽  
pp. e316
Author(s):  
T.-H. Lee ◽  
Y.-F. Shih ◽  
C.-C. Huang ◽  
M.-S. Lee
Keyword(s):  
Incubation Period ◽  
Sperm Capacitation ◽  
Short Incubation Period ◽  
Serum Supplementation ◽  
Synthetic Serum

scholarly journals Effects of Synthetic Serum Supplementation in Sperm Preparation Media on Sperm Capacitation and Function Test Results

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Ying-Fu Shih ◽  
Shu-Ling Tzeng ◽  
Wen-Jung Chen ◽  
Chun-Chia Huang ◽  
Hsiu-Hui Chen ◽  
...  
Keyword(s):  
Tyrosine Phosphorylation ◽  
Acrosome Reaction ◽  
Culture Media ◽  
Semen Analysis ◽  
Reaction Rates ◽  
Test Results ◽  
Sperm Capacitation ◽  
Serum Supplementation ◽  
Function Test ◽  
Synthetic Serum

Albumin supplementation of culture media induces sperm capacitation in assisted reproduction technique cycles. Synthetic serum supplementation is clinically used to replace albumin for preventing transmission of infectious agents. However, the effects of synthetic serum supplementation on sperm capacitation have rarely been investigated. Spermatozoa from 30 men with normal basic semen analysis results were collected, divided into five aliquots, and cultured in capacitating conditions in four combinations of two synthetic serum supplements, serum substitute supplement (SSS) and serum protein substitute (SPS), and two fertilization media, Quinns Advantage™Fertilization (QF) and human tubular fluid (HTF) media. Reactive oxygen species (ROS) levels in spermatozoa were measured through chemiluminescence. Furthermore, acrosome reaction and western blotting for tyrosine phosphorylation were used to evaluate sperm capacitation. HTF+SSS had significantly higher ROS levels than QF+SPS did (11,725 ± 1,172 versus 6,278 ± 864 relative light units). In addition, the spermatozoa cultured in QF+SPS had lower motility, acrosome reaction rates, and tyrosine phosphorylation levels compared with those cultured in HTF+SSS. In conclusion, the effects of synthetic serum supplementation on sperm capacitation varied according to the combination of media. These differences may lead to variations in spermatozoon ROS levels, thus affecting sperm function test results.

A bat rabies isolate with an unusually short incubation period

1980 ◽  
Vol 33 (2) ◽  
pp. 211-222 ◽  
Author(s):  
G.M. Baer ◽  
A.K. Harrison ◽  
S.P. Bauer ◽  
J.H. Shaddock ◽  
F.A. Murphy
Keyword(s):  
Incubation Period ◽  
Short Incubation Period ◽  
Bat Rabies

scholarly journals Effect of dimethylmaleate and phorone on glutathione content of Setaria cervi (Nematoda: Filarioidea) during in vitro incubation

2007 ◽  
Vol 44 (1) ◽  
pp. 14-17 ◽  
Author(s):  
S. Gupta ◽  
N. Banu ◽  
A. Srivastava
Keyword(s):  
Drug Design ◽  
Incubation Period ◽  
Salt Solution ◽  
Glutathione Metabolism ◽  
Setaria Cervi ◽  
Short Incubation Period ◽  
In Vitro Incubation ◽  
Dose Dependent

AbstractGlutathione metabolism represents a prospective target for antifilarial drug design, and therefore, the alterations in glutathione (GSH) content of filarial worms by known mammalian GSH depletors i.e. dimethylmaleate (DMM) and phorone were first thought for investigation in model filarial worms Setaria cervi. The dose dependent GSH depletion was achieved when these worms were incubated at 37°C for 6 h in Hanks balanced salt solution with varying concentrations (10–250 μM) of DMM or phorone. During the short incubation period of 6 h, 250 μM of DMM and phorone declined more than 90 % of the GSH content of filarial worms.

scholarly journals The Shanghai Pasteur Institute

1901 ◽  
Vol 1 (2) ◽  
pp. 260-268
Author(s):  
Arthur Stanley
Keyword(s):  
Incubation Period ◽  
Human Subject ◽  
Local Condition ◽  
Pasteur Institute ◽  
Short Incubation Period

The idea of having the means of applying the Anti-rabic treatment of Pasteur in Shanghai, China, had its origin in the marked incidence of rabies among the numerous dogs of the Shanghai district and the consequent large mortality from this disease. The local condition was the more marked because of the short incubation period observed in Shanghai both in the human subject and in the rabbit inoculated from the rabid dog, being seldom much over a month in man and rarely over two weeks in rabbits inoculated subdurally.

scholarly journals Inhibitory Effects of Iso-α and β Hop Acids Against Pediococcus pentosaceus

2019 ◽  
Vol 47 (4) ◽  
pp. 1316-1322
Author(s):  
Delia MICHIU ◽  
Frank DELVIGNE ◽  
Nicolas MABON ◽  
Mirela JIMBOREAN ◽  
Melinda FOGARASI ◽  
...  
Keyword(s):  
Cell Viability ◽  
Incubation Period ◽  
Acid Stress ◽  
Digital Object Identifier ◽  
Inhibitory Effects ◽  
Pediococcus Pentosaceus ◽  
Selective Media ◽  
Ph Values ◽  
Short Incubation Period ◽  
Stress Changes

The goal of the research was to assess the inhibitory effects of  hop extracts, iso-α and β acids, against Pediococcus pentosaceus bacteria, during a short incubation period, both in liquid selective media (high pH values) and beer wort fermentation (low pH values) and testing if the identified iso-α acid stress changes the activity of S. cerevisiae boulardii yeast and ethanol production. Flow cytometry analysis was used for bacterial and yeast cell viability. In relation to the antibacterial activity of β-acids, a lower viability of Pediococcus pentosaceus cells was observed after a short incubation period in selective media, under iso-α acid stress. In beer wort, for a mixed culture with P. pentosaceus bacteria and S. cerevisiae boulardii yeast, under iso-α acid stress conditions at pH 4.0-5.0, Pediococcus pentosaceus exhibited lower cell viability (20.7%) than in selective media (61.4%). Regarding iso-α hop acid on S. cerevisiae boulardii yeast, the results showed that iso-α does not change the S. cerevisiae activity but prevents the culture from being contaminated by Pediococcus pentosaceus. The results highlighted reliable inhibitory effects of iso-α and β-acids against P. pentosaceus, both at pH 6.0-7.0 and pH 4.0-5.0, which open the possibility of hops being used as a supplement to prevent beverage contamination with spoilage microorganisms.   ********* In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 4, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue. *********

A Case of Generalized Tetanus With a Short Incubation Period

2018 ◽  
pp. 64-66
Author(s):  
Fatih Temocin ◽  
◽  
Hatice Kose ◽  
Suleyman Sari ◽  
◽  
...  
Keyword(s):  
Incubation Period ◽  
Short Incubation Period

scholarly journals Tetanus in School Age Children Seen at the University of Port Harcourt Teaching Hospital: A Need for Booster Doses of Tetanus Vaccine

2020 ◽  
pp. 1-9
Author(s):  
B. A. Alex-Hart ◽  
D. LongJohn
Keyword(s):  
Incubation Period ◽  
Teaching Hospital ◽  
Case Fatality ◽  
School Age Children ◽  
School Age ◽  
Tetanus Vaccine ◽  
Short Incubation Period ◽  
Port Harcourt ◽  
The Mean ◽  
The University

Background: Tetanus still causes significant morbidity and mortality amongst children in Nigeria despite decades of immunisation with tetanus vaccine. Objectives: To determine the prevalence, case fatality rate and predictors of fatality amongst school age children treated for tetanus at the University of Port Harcourt Teaching Hospital. Materials and Methods:  This was a retrospective study of all children aged 4 to 17 years treated for tetanus at the University of Port Harcourt Teaching Hospital between January 1, 2009 and December 31, 2019. Data was obtained from the case notes and ward registers. Socio-demographic characteristics, presenting complaints, incubation period, onset interval, number of Diphtheria, Pertussis and Tetanus (DPT) vaccine received at infancy, treatment outcome, duration and cost of hospital stay were obtained, entered into a spread sheet and analysed with SPSS version 20. Results are presented in tables and percentages. The level of significance was set at P value <0.05. Results:  During the period under review, there were 53 children aged 4 to 17 years treated for tetanus, accounting for 0.3% of the total admission. The mean age was 10.79±3.35 years. There were 33 (62.26%) males and 20 (37.74%) females. Majority 26 (49.06%) of the children were of low socioeconomic class. The commonest 28(52.8%) route of infection was broomstick injury. Majority 32(60.3%) of the children were not immunised in infancy. The mean incubation period was 7.34±4.21 days and the mean onset interval was 8.87±10.44 hours. The two most common symptoms were generalized spasms 49 (21.03%) and trismus 47 (20.17%). Short incubation period (≤4 days) and short onset interval (≤4 hours) were significantly associated with higher death rates (p=0.0002; p=0.012).  Patients with short incubation period of ≤4 days and short onset interval of≤ 4 hours are more likely to die (p=0.0002; p=0.012). Conclusion: There was a high tetanus case fatality rate amongst school age children at University of Port Harcourt. Short incubation period and onset interval were associated with higher mortality. Booster doses of TT containing vaccines should be administered to primary and secondary school children in Port Harcourt to curb the menace.

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