scholarly journals Total phenolic and anthocyanin contents, as well as antioxidant activity, of black bean koji fermented by Aspergillus awamori under different culture conditions

2007 ◽  
Vol 104 (3) ◽  
pp. 936-942 ◽  
Author(s):  
I-Hsin Lee ◽  
Yu-Hsiang Hung ◽  
Cheng-Chun Chou
Keyword(s):  
Antioxidant Activity ◽  
Culture Conditions ◽  
Aspergillus Awamori ◽  
Total Phenolic ◽  
Black Bean

scholarly journals Induction and evaluation of secondary metabolite and antioxidant activity in adventitious root of Codonopsis javanica

2021 ◽  
Vol 63 (4) ◽  
pp. 11-16
Author(s):  
Thi Huong Trinh ◽  
◽  
Quoc Tuan Nguyen ◽  
Thi Huyen Trang Nguyen ◽  
Dang Giap Do ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Adventitious Root ◽  
Adventitious Roots ◽  
Large Scale ◽  
Culture Conditions ◽  
Total Phenolic ◽  
Root Induction ◽  
Material Source ◽  
Large Scale Systems ◽  
Dark Conditions

In this study, the effects of auxin (IBA, NAA), explants, and culture conditions (light/dark) on adventitious root induction of Codonopsis javanica were investigated. The results showed that dark conditions were more suitable for adventitious root induction than light conditions. All three types of explants (internodes, leaves, and nodes) induced adventitious roots, and the appropriate concentration of auxin was 0.5 mg/l IBA. After 4 weeks of incubation under dark conditions, the rooting percentage and number of roots/explant of internode, leaf, and node segments on media supplemented with 0.5 mg/l IBA were 100% and 33.87 roots, 97.78% and 23.48 roots, 100% and 25.20 roots, respectively. These adventitious roots were analysed for the presence of alkaloids, carbohydrates, saponin, fixed oils and fats, phenol, flavonoids, gum, and mucilage. The total polysaccharide content, total phenolic content, and the antioxidant activity (IC50) of C. javanica adventitious root biomass were 16.98%, 1.876 (mg GAE/g DW), and 2.44 (mg/ml), respectively. These results indicate that the adventitious roots of C. javanica contain bioactive compounds, which can be used as a material source for multiplication in large-scale systems.

scholarly journals Mild Salinity Stimulates Biochemical Activities and Metabolites Associated with Anticancer Activities in Black Horehound (Ballota nigra L.)

Agronomy ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 2538
Author(s):  
Mehdi Younessi-Hamzekhanlu ◽  
Zahra Dibazarnia ◽  
Shahin Oustan ◽  
Teniyah Vinson ◽  
Ramesh Katam ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Salt Stress ◽  
In Vitro Culture ◽  
Salinity Stress ◽  
Membrane Integrity ◽  
Morphological Characteristics ◽  
Culture Conditions ◽  
Salinity Level ◽  
Total Phenolic

Black horehound (Ballota nigra L.) is one of the most important medicinal plants, as a rich source of health-promoting essential oils and metabolites. Salinity stress affects plant development and alters antioxidant activity and plant metabolite composition. The present research aimed to study the effect of salinity on physiological and biochemical changes and metabolites of B. nigra under greenhouse and in vitro culture conditions. The plants were treated with different concentrations of NaCl (25, 50, 75, 100 mM), and morphological characteristics of the plant were measured. The growth-related traits and soil plant analysis development (SPAD) were decreased both in vivo and in vitro. Additionally, increased salt concentration negatively affected the cell membrane integrity. The total phenolic content and flavonoids of plants growing in the greenhouse increased by 21% at 50 mM of NaCl, but the amounts decreased significantly at higher stress levels (100 mM of NaCl). Antioxidant activity was also measured. Among the NaCl treatments, the most increased DPPH scavenging activities (IC50) under greenhouse and in vitro conditions were detected at mild salinity stress, but the activity significantly decreased in higher salinity treatments (i.e., 75 and 100 mM). In general, with increasing the salinity level to 75 mM, the activities of CAT and APX were significantly upregulated in both greenhouse and in vitro culture conditions. A correlation between total phenolics and flavonoids contents as well as antioxidant activity was obtained. Salinity level caused a shift in the metabolite expression. Mild salinity stress elevated the metabolites involved in anticancer and anti-inflammatory activities, such as β-ionone and caryophyllene oxide. However, the higher salt stress resulted in a significant reduction in their expression. Differential expression of metabolites to various levels of salt stress can be further exploited for the in vitro biosynthesis of metabolites.

LC-DAD-ESI-MS/MS, total phenolic content, and antioxidant activity of Firmiana simplex

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
MA Ghareeb ◽  
T Mohamed ◽  
AM Saad ◽  
LA Refahy ◽  
MA Sobeh ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Esi Ms

Evaluation of antioxidant and Anthelmintic activity of methanolic flower extract of Nyctanthes arbor-tristis

2018 ◽  
Vol 9 (1) ◽  
pp. 78
Author(s):  
Vijaya Jyothi M ◽  
Bhargav E ◽  
Pavan Kumar K ◽  
Praneeth Gowd K ◽  
Ram Pavan S
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Iridoid Glycosides ◽  
Radical Scavenging ◽  
Anthelmintic Activity ◽  
Total Phenolic ◽  
Standard Drug ◽  
Flower Extract ◽  
Reducing Ability ◽  
Total Flavonoids Content

Nyctanthes arbour-tristis is a shrub belongs to the family Oleaceae. The flowers of this plant are fragrant since the presence of flavonol glycosides. It has also been reported for the presence of β-sitosterol, iridoid glycosides, tannins etc., and known to have immunostimulant, hepatoprotective, antiviral and antifungal activities. In the present study an attempt is made to identify antioxidant capacity and anthelminthic potential of methanolic flower extract of Nyctanthes arbour-tristis. Antioxidant activity was evaluated by total phenolic content assay, total flavonoids content assay, free radical scavenging activity and reducing ability methods. Anthelmintic activity was evaluated on Perithima posthuma using Piperazine citrate as standard drug. The results obtained for the above activities reveals that Nyctanthes arbour-tristis shows considerable antioxidant activity for all the methods and anthelminthic potential at 300 mg/ml. Keywords: arbour-tristis; antioxidant activity; anthelminthic activity; Perithima posthuma; Piperazine citrate.

SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIOKSIDAN DAN ANTITUMOR 20 TUMBUHAN OBAT ASAL KABUPATEN MANOKWARI

2008 ◽  
Vol 7 (1) ◽  
Author(s):  
Bimo Budi Santoso
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Antitumor Activity ◽  
Traditional Method ◽  
Extraction Efficiency ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Total Phenolic Contents

<strong><em>Flavanoid, steroid, and terpenoid contents of 20 Manokwari medicinal plants were analyzed.  The antioxidant activity, total phenolic contents and antitumor activity of 20 Manokwari medicinal plants were also evaluated. The result shows that 83% positif to flavanoid test, 59% positif to terpenoid test and only 25% positif steroid. Antioxidant activity and total phenolic contents evaluated using Ferric Thiocianate (FTC) and Folin-Ciocalteu methods respectively. Antioxidant activity and total phenolic contents of medicinal plants were extracted by the traditional method, boiling in water and also in 80% methanol. Twenty plants evaluated in both exstracts have significantly varies of antioxidant activities and phenolic contents,  A significant and linier correlation coefficient between the antioxidant activity and the total phenolic content was found in both aqueous (R<sup>2</sup>= 0,77) and methanol (R<sup>2</sup> = 0,85). Antitumor activity was tested using cell maurine P-388 and only 2 of medicinal plants are active to inhibit cell maurine P-388. Comparing extraction efficiency of the two methods, the methanol extracted phenolic compounds more efficiently, and antioxidant activity of the extract was higher.</em></strong>

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