Identification of acacia honey treated with macroporous adsorption resins using HPLC-ECD and chemometrics

This study reports an analytical method for the determination of nitroimidazole and quinolones in honey using liquid chromatography-tandem mass spectrometry (LC-MS/MS). A modified QuEChERS methodology was used to extract the analytes and determine veterinary drugs in honey by LC-MS/MS. The linear regression was excellent at the concentration levels of 1–100 ng/mL in the solution standard curve and the matrix standard curve. The recovery rates of nitroimidazole and quinolones were 4.4% to 59.1% and 9.8% to 46.2% with relative standard deviations (RSDs) below 5.2% and the recovery rates of nitroimidazole and quinolones by the matrix standard curve ranged from 82.0% to 117.8% and 79% to 115.9% with relative standard deviations (RSDs) lower than 6.3% in acacia and jujube honey. The acacia and jujube honeys have stronger matrix inhibition effect to nitroimidazole and quinolones residue; the matrix inhibition effect of jujube honey is stronger than acacia honey. The matrix standard curve can calibrate matrix effect effectively. In this study, the detection method of antibiotics in honey can be applied to the actual sample. The results demonstrated that the modified QuEChERS method combined with LC-MS/MS is a rapid, high, sensitive method for the analysis of nitroimidazoles and quinolones residues in honey.

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In-situ measurement of Auramine-O adsorption on macroporous adsorption resins at low temperature using fiber-optic sensing

10.5004/dwt.2021.26675 ◽

2021 ◽

Vol 213 ◽

pp. 240-247

Author(s):

Tursunjan Aydan ◽

Jing-Jing Yang ◽

Turghun Muhammad ◽

Fei Gao ◽

Xiao-Xia Yang ◽

...

Keyword(s):

Low Temperature ◽

Fiber Optic ◽

In Situ Measurement ◽

Auramine O ◽

Macroporous Adsorption Resins ◽

Fiber Optic Sensing

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Antibacterial properties of Kelulut, Tualang and Acacia honey against fourteen clinically-isolated strains of bacteria-infecting wound

10.1063/5.0020300 ◽

2020 ◽

Author(s):

Mohd-Amir Mohd ◽

Raihana Edros ◽

Norul A. Hamzah

Keyword(s):

Antibacterial Properties ◽

Acacia Honey

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ACACIA HONEY INDUCES APOPTOSIS IN HUMAN BREAST ADENOCARCINOMA CELL LINES (MCF-7)

Jurnal Teknologi ◽

10.11113/jt.v79.9882 ◽

2017 ◽

Vol 79 (4) ◽

Author(s):

Muhammad Ashraf Mohd Salleh ◽

Zolkapli Eshak ◽

Wan Iryani Wan Ismail

Keyword(s):

Antioxidant Properties ◽

Breast Adenocarcinoma ◽

Total Phenolic ◽

Flavonoid Content ◽

Cell Shrinkage ◽

Cancer Cell Growth ◽

Breast Cancer Cell Growth ◽

Acacia Honey ◽

Induced Apoptosis ◽

Mcf 7

Apoptosis is one of markers considered in drug design including in treating cancers. Conventional treatments for cancer cause various side effects. One of possible alternatives is honey, an antioxidant driven by its phenolic and flavonoids content. This study aims to observe the effects of Malaysian Acacia honey in inhibiting breast cancer cell growth through apoptosis. Antioxidant properties of the honey were measured using total phenolic content (TPC) and total flavonoid content (TFC). Then, antiproliferative activity of the honey were observed using MTT assay. The honey exhibited IC50 at concentration of 5.5% (v/v). Further studies using TUNEL and live cell view revealed that Acacia honey induced apoptosis after 6 hours of treatment. Cell shrinkage, which is one of the apoptotic features, was observed as early as 2 hours, followed by the formation of apoptotic bodies within 6 hours of the honey treatment. Details of mechanism and actual compounds involved in displaying the results are being intensively studied.

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Discrimination of geographical origins of Chinese acacia honey using complex 13C/12C, oligosaccharides and polyphenols

Food Chemistry ◽

10.1016/j.foodchem.2018.07.227 ◽

2019 ◽

Vol 272 ◽

pp. 580-585 ◽

Cited By ~ 15

Author(s):

Seng She ◽

Lanzhen Chen ◽

Hongbo Song ◽

Guanghui Lin ◽

Yi Li ◽

...

Keyword(s):

Acacia Honey

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Changes of antioxidant activity in honey after heat treatment

Czech Journal of Food Sciences ◽

10.17221/509/2012-cjfs ◽

2013 ◽

Vol 31 (No. 6) ◽

pp. 601-606 ◽

Cited By ~ 8

Author(s):

G. Šarić ◽

K. Marković ◽

D. Vukičević ◽

E. Lež ◽

M. Hruškar ◽

...

Keyword(s):

Antioxidant Activity ◽

Total Phenolic Content ◽

Phenolic Content ◽

Total Phenolic ◽

Antioxidant Power ◽

Dpph Method ◽

Significant Difference ◽

Acacia Honey ◽

Different Temperatures ◽

Ferric Reducing Antioxidant Power

We determined how the antioxidant activity and total phenolic content of honey changed after being subjected to a high temperature. Antioxidant activity was determined using two methods – FRAP (ferric reducing antioxidant power) and DPPH (1,1-diphenyl-2-picrylhydrazyl) assays. Total phenolic content was determined by modified Folin-Ciocalteu method. The research was conducted on 31 samples of acacia honey and 8 samples of chestnut honey. All measurements were done at two temperatures – at 23°C (room temperature) and after 5 min of heating at 95°C. The obtained results show uneven changes of antioxidant activity and total phenolic content among individual samples, i.e. in some samples antioxidant activity decreased after heating, while in others it increased. The same applies to the total phenolic content. Statistical analysis of the results (t-test) showed no statistically significant differences between the results measured at two different temperatures (P > 0.05) in all three methods used, and in both types of honey. The only statistically significant difference (P < 0.05) was observed when using DPPH method in acacia honey.

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Antioxidant Activities and Total Phenolics of Acacia Honey

Czech Journal of Food Sciences ◽

10.17221/1112-cjfs ◽

2009 ◽

Vol 27 (Special Issue 1) ◽

pp. S245-S247 ◽

Cited By ~ 17

Author(s):

M. Krpan ◽

K. Marković ◽

G. Šarić ◽

B. Skoko ◽

M. Hruškar ◽

...

Keyword(s):

Antioxidant Activity ◽

Total Phenolic Content ◽

Antiradical Activity ◽

Phenolic Content ◽

Antioxidant Activities ◽

Optical Rotation ◽

Total Phenolic ◽

Antioxidant Power ◽

Dpph Method ◽

Acacia Honey

The antioxidant activities and total phenolic content of 30 samples of acacia honey from Croatian territory were analysed. Phenolics were determined by the modified Folin-Ciocalteu method, antiradical activity by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and potential antioxidant activity using the ferric reducing antioxidant power (FRAP) method. In all samples, physicochemical parameters (water content, electrical conductivity, total reducing sugars, sucrose content, acidity, hydroxymethylfurfural content, prolin content, optical rotation, diastase activity and invertase activity) were measured according to Croatian legislation and International regulatory standards. Honey can be considered as a dietary supplement as it contains some important components including α-tocopherol, ascorbic acid, flavonoids and phenolics. The composition and properties of honey are dependent on floral origins, climatic conditions of the produced area, processing and storage methods. The results of physicochemical analyses showed that all the values of investigated parameters are in agreement with the current legislation. Phenolic content ranged from 31.72 mg/kg to 80.11 mg/kg, antiradical activity expressed as IC50 ranged from 61.28% to 253.47% and antioxidant activity expressed as FRAP value from 6.95 to 142.43. A positive correlation was observed between total phenolic content and antioxidant activity, indicating that phenolic compounds are mainly responsible for the antioxidant power of acacia honey.

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