Discrimination of geographical origins of Chinese acacia honey using complex 13C/12C, oligosaccharides and polyphenols

This study reports an analytical method for the determination of nitroimidazole and quinolones in honey using liquid chromatography-tandem mass spectrometry (LC-MS/MS). A modified QuEChERS methodology was used to extract the analytes and determine veterinary drugs in honey by LC-MS/MS. The linear regression was excellent at the concentration levels of 1–100 ng/mL in the solution standard curve and the matrix standard curve. The recovery rates of nitroimidazole and quinolones were 4.4% to 59.1% and 9.8% to 46.2% with relative standard deviations (RSDs) below 5.2% and the recovery rates of nitroimidazole and quinolones by the matrix standard curve ranged from 82.0% to 117.8% and 79% to 115.9% with relative standard deviations (RSDs) lower than 6.3% in acacia and jujube honey. The acacia and jujube honeys have stronger matrix inhibition effect to nitroimidazole and quinolones residue; the matrix inhibition effect of jujube honey is stronger than acacia honey. The matrix standard curve can calibrate matrix effect effectively. In this study, the detection method of antibiotics in honey can be applied to the actual sample. The results demonstrated that the modified QuEChERS method combined with LC-MS/MS is a rapid, high, sensitive method for the analysis of nitroimidazoles and quinolones residues in honey.

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Antibacterial properties of Kelulut, Tualang and Acacia honey against fourteen clinically-isolated strains of bacteria-infecting wound

10.1063/5.0020300 ◽

2020 ◽

Author(s):

Mohd-Amir Mohd ◽

Raihana Edros ◽

Norul A. Hamzah

Keyword(s):

Antibacterial Properties ◽

Acacia Honey

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ACACIA HONEY INDUCES APOPTOSIS IN HUMAN BREAST ADENOCARCINOMA CELL LINES (MCF-7)

Jurnal Teknologi ◽

10.11113/jt.v79.9882 ◽

2017 ◽

Vol 79 (4) ◽

Author(s):

Muhammad Ashraf Mohd Salleh ◽

Zolkapli Eshak ◽

Wan Iryani Wan Ismail

Keyword(s):

Antioxidant Properties ◽

Breast Adenocarcinoma ◽

Total Phenolic ◽

Flavonoid Content ◽

Cell Shrinkage ◽

Cancer Cell Growth ◽

Breast Cancer Cell Growth ◽

Acacia Honey ◽

Induced Apoptosis ◽

Mcf 7

Apoptosis is one of markers considered in drug design including in treating cancers. Conventional treatments for cancer cause various side effects. One of possible alternatives is honey, an antioxidant driven by its phenolic and flavonoids content. This study aims to observe the effects of Malaysian Acacia honey in inhibiting breast cancer cell growth through apoptosis. Antioxidant properties of the honey were measured using total phenolic content (TPC) and total flavonoid content (TFC). Then, antiproliferative activity of the honey were observed using MTT assay. The honey exhibited IC50 at concentration of 5.5% (v/v). Further studies using TUNEL and live cell view revealed that Acacia honey induced apoptosis after 6 hours of treatment. Cell shrinkage, which is one of the apoptotic features, was observed as early as 2 hours, followed by the formation of apoptotic bodies within 6 hours of the honey treatment. Details of mechanism and actual compounds involved in displaying the results are being intensively studied.

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Changes of antioxidant activity in honey after heat treatment

Czech Journal of Food Sciences ◽

10.17221/509/2012-cjfs ◽

2013 ◽

Vol 31 (No. 6) ◽

pp. 601-606 ◽

Cited By ~ 8

Author(s):

G. Šarić ◽

K. Marković ◽

D. Vukičević ◽

E. Lež ◽

M. Hruškar ◽

...

Keyword(s):

Antioxidant Activity ◽

Total Phenolic Content ◽

Phenolic Content ◽

Total Phenolic ◽

Antioxidant Power ◽

Dpph Method ◽

Significant Difference ◽

Acacia Honey ◽

Different Temperatures ◽

Ferric Reducing Antioxidant Power

We determined how the antioxidant activity and total phenolic content of honey changed after being subjected to a high temperature. Antioxidant activity was determined using two methods – FRAP (ferric reducing antioxidant power) and DPPH (1,1-diphenyl-2-picrylhydrazyl) assays. Total phenolic content was determined by modified Folin-Ciocalteu method. The research was conducted on 31 samples of acacia honey and 8 samples of chestnut honey. All measurements were done at two temperatures – at 23°C (room temperature) and after 5 min of heating at 95°C. The obtained results show uneven changes of antioxidant activity and total phenolic content among individual samples, i.e. in some samples antioxidant activity decreased after heating, while in others it increased. The same applies to the total phenolic content. Statistical analysis of the results (t-test) showed no statistically significant differences between the results measured at two different temperatures (P > 0.05) in all three methods used, and in both types of honey. The only statistically significant difference (P < 0.05) was observed when using DPPH method in acacia honey.

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Antioxidant Activities and Total Phenolics of Acacia Honey

Czech Journal of Food Sciences ◽

10.17221/1112-cjfs ◽

2009 ◽

Vol 27 (Special Issue 1) ◽

pp. S245-S247 ◽

Cited By ~ 17

Author(s):

M. Krpan ◽

K. Marković ◽

G. Šarić ◽

B. Skoko ◽

M. Hruškar ◽

...

Keyword(s):

Antioxidant Activity ◽

Total Phenolic Content ◽

Antiradical Activity ◽

Phenolic Content ◽

Antioxidant Activities ◽

Optical Rotation ◽

Total Phenolic ◽

Antioxidant Power ◽

Dpph Method ◽

Acacia Honey

The antioxidant activities and total phenolic content of 30 samples of acacia honey from Croatian territory were analysed. Phenolics were determined by the modified Folin-Ciocalteu method, antiradical activity by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and potential antioxidant activity using the ferric reducing antioxidant power (FRAP) method. In all samples, physicochemical parameters (water content, electrical conductivity, total reducing sugars, sucrose content, acidity, hydroxymethylfurfural content, prolin content, optical rotation, diastase activity and invertase activity) were measured according to Croatian legislation and International regulatory standards. Honey can be considered as a dietary supplement as it contains some important components including α-tocopherol, ascorbic acid, flavonoids and phenolics. The composition and properties of honey are dependent on floral origins, climatic conditions of the produced area, processing and storage methods. The results of physicochemical analyses showed that all the values of investigated parameters are in agreement with the current legislation. Phenolic content ranged from 31.72 mg/kg to 80.11 mg/kg, antiradical activity expressed as IC50 ranged from 61.28% to 253.47% and antioxidant activity expressed as FRAP value from 6.95 to 142.43. A positive correlation was observed between total phenolic content and antioxidant activity, indicating that phenolic compounds are mainly responsible for the antioxidant power of acacia honey.

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Characterization and classification of Romanian acacia honey based on its physicochemical parameters and chemometrics

Scientific Reports ◽

10.1038/s41598-020-77685-9 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Mihaela Emanuela Crăciun ◽

Oana Cristina Pârvulescu ◽

Andreea Cristina Donise ◽

Tănase Dobre ◽

Dumitru Radu Stanciu

Keyword(s):

Physicochemical Parameters ◽

Stable Carbon Isotopes ◽

Reducing Sugars ◽

Activity Ratio ◽

Sugar Content ◽

Principal Component ◽

Reducing Sugar ◽

Linear Discriminant ◽

Acacia Honey

AbstractThree groups of Romanian acacia honey, i.e., pure, directly adulterated (by mixing the pure honey with three sugar syrups), and indirectly adulterated (by feeding the bees with the same syrups), were characterized and discriminated based on their physicochemical parameters. Moisture, ash, 5-hydroxymethylfurfural (HMF), reducing sugars (fructose and glucose), and sucrose contents, free acidity, diastase activity, ratio between stable carbon isotopes of honey and its proteins (δ13CH and δ13CP) were evaluated. Adulteration led to a significant increase in sucrose content, HMF level, and Δδ13C = δ13CH‒δ13CP as well a decrease in reducing sugar content and diastase activity. Principal component analysis (PCA) and linear discriminant analysis (LDA) were applied to experimental data in order to distinguish between pure and adulterated honey. The most relevant discriminative parameters were diastase activity, HMF, sucrose, and reducing sugar contents. Posterior classification probabilities and classification functions obtained by LDA revealed that 100% of honey samples were correctly assigned to their original group.

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Discrimination of Natural Mature Acacia Honey Based on Multi-Physicochemical Parameters Combined with Chemometric Analysis

Molecules ◽

10.3390/molecules24142674 ◽

2019 ◽

Vol 24 (14) ◽

pp. 2674 ◽

Cited By ~ 3

Author(s):

Tianchen Ma ◽

Haoan Zhao ◽

Caiyun Liu ◽

Min Zhu ◽

Hui Gao ◽

...

Keyword(s):

Principal Component Analysis ◽

Physicochemical Parameters ◽

Phenolic Content ◽

Principal Component ◽

Component Analysis ◽

Chemometric Analysis ◽

Total Phenolic ◽

Total Protein Content ◽

Acacia Honey

Honey maturity is an important factor in evaluating the quality of honey. We established a method for the identification of natural mature acacia honey with eighteen physicochemical parameters combined with chemometric analysis. The analysis of variance showed significant differences between mature and immature acacia honey in physicochemical parameters. The principal component analysis explained 82.64% of the variance among samples, and indicated that total phenolic content, total protein content, and total sugar (glucose, fructose, sucrose) were the major variables. The cluster analysis and orthogonal partial least squares-discriminant analysis demonstrated that samples were grouped in relation to the maturity coinciding with the results of the principal component analysis. Meanwhile, the 35 test samples were classified with 100% accuracy with the method of multi-physicochemical parameters combined with chemometric analysis. All the results presented above proved the possibility of identifying mature acacia honey and immature acacia honey according to the chemometric analysis based on the multi-physicochemical parameters.

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