ACACIA HONEY INDUCES APOPTOSIS IN HUMAN BREAST ADENOCARCINOMA CELL LINES (MCF-7)

2017 ◽  
Vol 79 (4) ◽  
Author(s):  
Muhammad Ashraf Mohd Salleh ◽  
Zolkapli Eshak ◽  
Wan Iryani Wan Ismail
Keyword(s):  
Antioxidant Properties ◽  
Breast Adenocarcinoma ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Cell Shrinkage ◽  
Cancer Cell Growth ◽  
Breast Cancer Cell Growth ◽  
Acacia Honey ◽  
Induced Apoptosis ◽  
Mcf 7

Apoptosis is one of markers considered in drug design including in treating cancers. Conventional treatments for cancer cause various side effects. One of possible alternatives is honey, an antioxidant driven by its phenolic and flavonoids content. This study aims to observe the effects of Malaysian Acacia honey in inhibiting breast cancer cell growth through apoptosis. Antioxidant properties of the honey were measured using total phenolic content (TPC) and total flavonoid content (TFC). Then, antiproliferative activity of the honey were observed using MTT assay. The honey exhibited IC50 at concentration of 5.5% (v/v). Further studies using TUNEL and live cell view revealed that Acacia honey induced apoptosis after 6 hours of treatment. Cell shrinkage, which is one of the apoptotic features, was observed as early as 2 hours, followed by the formation of apoptotic bodies within 6 hours of the honey treatment. Details of mechanism and actual compounds involved in displaying the results are being intensively studied.

scholarly journals ANTIOXIDANT PROFILE AND PHYTOCHEMICAL CONTENT OF DIFFERENT PARTS OF SUPER RED DRAGON FRUIT (HYLOCEREUS COSTARICENSIS) COLLECTED FROM WEST JAVA-INDONESIA

2017 ◽  
Vol 10 (12) ◽  
pp. 290 ◽  
Author(s):  
Irda Fidrianny ◽  
Nadia Ilham ◽  
Rika Hartati
Keyword(s):  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Phytochemical Content ◽  
Dragon Fruit ◽  
Dpph Method ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
Different Parts

Objectives: The goals of this research were to observe antioxidant properties from different parts of super red dragon fruit (Hylocereus costaricensis) using two antioxidant testing methods which were 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS).Methods: Antioxidant activities were determined using DPPH and ABTS assays, total phenolic content (TPC) using Folin–Ciocalteu reagent, flavonoid content by Chang’s method.Results: Inhibitory concentration 50% (IC50) of DPPH scavenging activity of all of the extracts in the range of 2.69 μg/ml was −94.17 μg/ml. The ethyl acetate peel extract of super red dragon fruit expressed the highest TPC (4.56 g GAE/100 g) and the highest total flavonoid content (12.63 g QE/100 g). TPC in flesh extract of super red dragon fruit had a negative and significant correlation with their IC50 of ABTS. The IC50 of DPPH and IC50 of ABTS of flesh extract of super red dragon fruit showed positive and significant correlation.Conclusion: All different parts extracts of super red dragon fruit (except n-hexane flesh extract) were categorized as a very strong antioxidant by DPPH method. Phenolic compounds in flesh extract of super red dragon fruit were the major contributor in antioxidant activities by ABTS method. DPPH and ABTS showed linear results in antioxidant activities of super red dragon fruit flesh extract.

ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF RUTA ANGUSTIFOLIA EXTRACT

2015 ◽  
Vol 77 (25) ◽  
Author(s):  
Nur Azfa Shuib ◽  
Anwar Iqbal ◽  
Fatimatul Akmal Sulaiman ◽  
Izzatie Razak ◽  
Deny Susanti
Keyword(s):  
Antibacterial Activity ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Disc Diffusion ◽  
Flavonoid Content ◽  
Disc Diffusion Method ◽  
Polyphenolic Content

Ruta angustifolia was used in this study in order to evaluate the antimicrobial activity and antioxidant properties and its correlation with the polyphenolic content. Two Gram-positive bacteria (Staphylococcus aureus ATCC 25923, Bacillus cereus ATCC 11778) and two Gram–negative bacteria (Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 8739) were used to determine the antibacterial activity. Aqueous maceration extract was used for antioxidant activities and methanolic maceration extract was used for antibacterial activity. The antioxidant properties and activities were evaluated by using total phenolic content (TPC), total flavonoid content (TFC), DPPH free radical scavenging activity and beta-carotene bleaching method. Whereas, the antibacterial activity was examined using disc diffusion method against selected microorganism at concentration 1.0 mg/disc. The results showed the phenolic content of R. angustifolia extract was 18.89 g GAE/100 g extract while the flavonoid content was 14.170 g QE/100 g extract. R. angustifolia exhibited good radical scavenging with IC50 value of 2.04 mg/ml. The result for disc diffusion method showed no inhibition zone against all the strains of bacteria at 1.0 mg/disc concentration of the extract. Based on the results, it can be concluded that the R. angustifolia aqueous extract has the antioxidant properties and there is correlation between polyphenolic content of the extract with its antioxidant activity. However, R. angustifolia methanolic extract did not show any antibacterial activity.

scholarly journals LC-ESI-QTOF/MS Characterisation of Phenolic Acids and Flavonoids in Polyphenol-Rich Fruits and Vegetables and Their Potential Antioxidant Activities

Antioxidants ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 405 ◽  
Author(s):  
Chunhe Gu ◽  
Kate Howell ◽  
Frank R. Dunshea ◽  
Hafiz A. R. Suleria
Keyword(s):  
Liquid Chromatography ◽  
Phenolic Acids ◽  
Fruits And Vegetables ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Tannin Content ◽  
Antioxidant Power ◽  
Plant Foods

Polyphenols are naturally occurring compounds found largely in fruits and vegetables. The antioxidant properties of these polyphenols including total phenolic content (TPC), total flavonoid content (TFC), tannin content, 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH), 2,2′-azinobis-(3-ethylbenzo-thiazoline-6-sulfonic acid) (ABTS) scavenging abilities and ferric ion reducing antioxidant power (FRAP) were measured among sixteen (16) plant foods (mango, blueberry, strawberry, black carrot, raspberry, dark grapes, garlic, ginger, onion, cherry, plum, apple, papaya, peach, pear and apricot) by modifying, standardising and translating existing antioxidant methods using a 96-well plate reader. Eighteen targeted phenolic acids and flavonoids were characterised and quantified using high-performance liquid chromatography-photometric diode array (HPLC-PDA) and verified by modifying an existing method of liquid chromatography coupled with electrospray-ionisation triple quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF/MS). While most of these compounds were accurately detected by the HPLC-PDA at a low concentration, a few polyphenols in low concentrations could be only be characterised using the LC-ESI-QTOF/MS method. Our results showed that mango possessed the highest overall antioxidant activity, phenolic acid and flavonoid content among the selected fruits. Factor analysis (FA) and Pearson’s correlation tests showed high correlations among ABTS, DPPH, FRAP and phenolic acids, implying the comparable capabilities of scavenging the DPPH/ABTS free radicals and reducing ferric ions from the antioxidant compounds in the samples. Phenolic acids contributed significantly to the antioxidant activities, and flavonoids contributed more to tannin content based on the correlations. Overall, methods modified and standardized in this study can provide better understanding of high throughput technologies and increase the reliability of antioxidant data of different plant foods.

scholarly journals Arsenic trioxide inhibits breast cancer cell growth via microRNA-328/hERG pathway in MCF-7 cells

2012 ◽  
Vol 12 (1) ◽  
pp. 1233-1238 ◽  
Author(s):  
YING WANG ◽  
LEQIU WANG ◽  
CHANGHAO YIN ◽  
BAIZHU AN ◽  
YANKUN HAO ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Growth ◽  
Arsenic Trioxide ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cancer Cell Growth ◽  
Breast Cancer Cell Growth ◽  
Mcf 7

scholarly journals Comparative study of the antioxidant properties of silymarin and milk thistle extracts

2021 ◽  
Vol 19 (1) ◽  
pp. 1-6
Author(s):  
V. Ivanov ◽  
M. Pavlova
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Water Extract ◽  
Total Phenolic ◽  
Milk Thistle ◽  
Flavonoid Content ◽  
Silybum Marianum ◽  
Dpph Method ◽  
Phenolic And Flavonoid

Purpose: Milk thistle (Silybum marianum (L.) Gaertn) has been used for centuries as a natural remedy for liver and gall bladder / biliary tract diseases. Silymarin is the purified and standardized extract from milk thistle seeds. The purpose of the present study is to present a comparative analysis of the total phenolic substances content and the antioxidant effect of silymarin and three different types of Silybum marianum extracts (i.e. ethanol extract, water extract and lyophilized extract). Methods: Total phenolic and flavonoid content of extracts was analysed and the antioxidant activity was assessed by determining their free radical scavenging ability by DPPH method. Results: Total phenolic content is the highest in silymarin– 134.12±3.22 mg GAE/g, followed by lyophilized water extract – 51.74±2.10 mg GAE/g. Silymarin has also been found to have the highest antioxidant activity among all extract samples (338.03 VCEAC µmol /g), followed by S.marianum lyophilised extract (123.05 VCEAC µmol/g). Conclusions: A strong correlation between total phenolic and flavonoid content in the extracts and their antioxidant activity has been found.

scholarly journals Evaluation of The Physicochemical, Sensorial and Antioxidant Properties of Functional Ale Beer Brewed with Rice and Fruit by-Products

2021 ◽  
Vol 20 (2) ◽  
Author(s):  
Wichien Sriwichai ◽  
Pakkawat Detchewa ◽  
Patcharee Prasajak
Keyword(s):  
Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Principal Component ◽  
Banana Peel ◽  
Total Phenolic ◽  
Malt Extract ◽  
Flavonoid Content ◽  
Coffee Pulp ◽  
By Products ◽  
Rice Powder

Functional beer brewed with a high bioactive compound ingredients is beneficial to prevent many diseases. Rice and fruit by-products are rich in bioactive compounds and have a potential for functional beer production. The aim of the present work was to assess the physicochemical, nutritional and sensorial properties of the beer brewed with rice and fruit by-products. The five formulas of beer investigated were the dried malt extract, Phitsanulok paddy rice powder, riceberry powder, banana peel and coffee pulp. The ratio of the dried malt extract and the other alternative ingredients was 80:20. The contents in reducing sugar, total phenolic compounds, flavonoid, chlorogenic acid, caffeine and dietary fiber were evaluated. The correlations between the physicochemical, sensorial, antioxidant properties and acceptability of beers were assessed by the Principal Component Analysis. The results showed that beers were differed in color density and have a low alcohol content. Among beer formula, beers brewed with coffee pulp was the richest in total phenolic and beers brewed with banana peel was the richest in flavonoid content (278.82 mg gallic acid equivalent/ L beer and 69.18 mg catechin equivalent/ L respectively). The antioxidant capacity was the highest in banana peel beer formula (76.64%). The Principal Component Analysis revealed that beers brewed with the two varieties of rice powder formula were in the same group of beer control (dried malt extract formula). These beers were characterized by a high note of appreciation from consumer. Whereas, a high correlation between the antioxidant capacity and total polyphenolic, flavonoid content was observed in beer fruit by-products formulas. The incorporation of two varieties of rice powder or fruit by-products was feasible in brewing process to produce a functional ale beer appreciated by customer and having a noticeable antioxidant capacity.

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