Ultra-efficient antimicrobial photodynamic inactivation system based on blue light and octyl gallate for ablation of planktonic bacteria and biofilms of Pseudomonas fluorescens

2021 ◽  
pp. 131585
Author(s):  
Yu-gang Shi ◽  
Lai Jiang ◽  
Shan Lin ◽  
Wen-gang Jin ◽  
Qing Gu ◽  
...  
Keyword(s):  
Pseudomonas Fluorescens ◽  
Blue Light ◽  
Photodynamic Inactivation ◽  
Planktonic Bacteria

Photodynamic Inactivation of Bacteria In Vitro Under the Effect of Blue Light

2015 ◽  
Vol 158 (4) ◽  
pp. 475-477 ◽  
Author(s):  
E. S. Keshishyan ◽  
Z. V. Zaporozhtseva ◽  
O. M. Zenina ◽  
V. S. Zrodnikov
Keyword(s):  
Blue Light ◽  
Photodynamic Inactivation ◽  
Inactivation Of Bacteria

scholarly journals Improving vaccine efficacy based on non-covalent photoinactivation of microorganisms - sources vaccine antigens (MSVA)

2020 ◽  
Author(s):  
Artur Martynov ◽  
Boris Farber ◽  
Tatyana Osolodchenko ◽  
Ilya Klein
Keyword(s):  
Protective Effect ◽  
Blue Light ◽  
Bacterial Growth ◽  
Final Concentration ◽  
Control Group ◽  
Photodynamic Inactivation ◽  
E Coli ◽  
Light Emitter ◽  
Vaccine Antigens ◽  
Inactivated Vaccines

AbstractOne of the most promising methods for non-covalent inactivation of vaccine-producing microorganisms is the use of photoinactivation using riboflavin derivatives. The study used a dynamic combinatorial derivative of riboflavin - succinyl-maleinyl riboflavin. Corpuscular vaccines are divided into the following groups: from 2AB to 5AB - bacteria were inactivated by riboflavin derivative and blue light, and groups from 6AB to 9AB were inactivated by formalin (0.1% formalin in 9 log CFU was kept for 2 weeks in an thermostat and then sterility was determined - bacterial growth was not observed). A dynamic derivative of riboflavin at a final concentration of 0.02% can photo inactivate 6 time more bacteria P. Aeruginosa and E. coli than riboflavin. The minimum effective blue light emitter power (450 nm) for the photodynamic inactivation of both P. aeruginosa and E. coli is 1024.2 mW / cm2. In groups of mice pre-vaccinated intraperitoneally with corpuscular photo inactivated vaccines based on suspensions of and E. coli at doses of 0.5-1.0 ml 4 log (CFU) / mL, 100% survival of all animals was observed, whereas in control group with the same type of vaccines but formalin-treated vaccines, it failed to achieve a 100% protective effect.

scholarly journals Photodynamic inactivation of Pseudomonas fluorescens in Minas Frescal cheese using curcumin as a photosensitizer

LWT ◽  
2021 ◽  
pp. 112143
Author(s):  
Bruna Barnei Saraiva ◽  
Bruna Moura Rodrigues ◽  
Ranulfo Combuca da Silva Junior ◽  
Mônica Regina da Silva Scapim ◽  
Cesar Armando Contreras Lancheros ◽  
...  
Keyword(s):  
Pseudomonas Fluorescens ◽  
Photodynamic Inactivation

scholarly journals Photoinactivation of Catalase Sensitizes Wide-Ranging Bacteria to ROS-Producing Agents and Immune Cells

2021 ◽  
Author(s):  
Pu-Ting Dong ◽  
Sebastian Jusuf ◽  
Jie Hui ◽  
Yuewei Zhan ◽  
Yifan Zhu ◽  
...  
Keyword(s):  
Blue Light ◽  
Immune Cells ◽  
Bacterial Infections ◽  
Multidrug Resistant ◽  
Aerobic Bacteria ◽  
Oxygen Species ◽  
Wild Type ◽  
Planktonic Bacteria ◽  
Wide Range ◽  
Molecular Components

Bacteria have evolved to cope with the detrimental effects of reactive oxygen species (ROS) using their essential molecular components. Catalase, a heme-containing tetramer protein expressed universally in most of the aerobic bacteria, plays an indispensable role in scavenging excess hydrogen peroxide (H2O2). Here, through utilization of wild-type and catalase-deficient mutants, we identified catalase as an endogenous therapeutic target of 400-420 nm blue light. Catalase residing in bacteria could be effectively inactivated by blue light, subsequently rendering the pathogens extremely vulnerable to H2O2 and H2O2-producing agents. As a result, photoinactivation of catalase and H2O2 synergistically eliminate a wide range of catalase-positive planktonic bacteria and P. aeruginosa inside biofilms. In addition, photoinactivation of catalase is shown to facilitate macrophages to defend against intracellular pathogens. The antimicrobial efficacy of catalase photoinactivation is further validated using a Pseudomonas aeruginosa-induced mice abrasion model. Taken together, our findings offer a catalase-targeting phototherapy against multidrug-resistant bacterial infections.

Photodynamic inactivation against Pseudomonas aeruginosa by curcumin microemulsions

RSC Advances ◽  
2016 ◽  
Vol 6 (67) ◽  
pp. 63013-63022 ◽  
Author(s):  
Chi-Hsien Liu ◽  
Wei-Shiou Lee ◽  
Wei-Chi Wu
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Blue Light ◽  
Photodynamic Inactivation ◽  
Light Diode

Photodynamic inhibition of Pseudomonas aeruginosa is confirmed by curcumin microemulsion through the help of the blue light diode.

scholarly journals Development of Staphylococcus aureus tolerance to antimicrobial photodynamic inactivation and antimicrobial blue light upon sub-lethal treatment

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Aleksandra Rapacka-Zdonczyk ◽  
Agata Wozniak ◽  
Michal Pieranski ◽  
Anna Woziwodzka ◽  
Krzysztof P. Bielawski ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Blue Light ◽  
Photodynamic Inactivation

scholarly journals Surface Modification of Nanocrystalline TiO2 Materials with Sulfonated Porphyrins for Visible Light Antimicrobial Therapy

Catalysts ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 821 ◽  
Author(s):  
Adam Sułek ◽  
Barbara Pucelik ◽  
Marcin Kobielusz ◽  
Przemysław Łabuz ◽  
Grzegorz Dubin ◽  
...  
Keyword(s):  
Blue Light ◽  
Active Surface ◽  
Sem Analysis ◽  
Hybrid Nanoparticles ◽  
Photodynamic Inactivation ◽  
Nanocrystalline Tio2 ◽  
Gram Positive Bacteria ◽  
Highly Active ◽  
Surface Modified ◽  
20 Nm

Highly-active, surface-modified anatase TiO2 nanoparticles were successfully synthesized and characterized. The morphological and optical properties of the obtained (metallo)porphyrin@qTiO2 materials were evaluated using absorption and fluorescence spectroscopy, scanning electron microscopy (SEM) imaging, and dynamic light scattering (DLS). These hybrid nanoparticles efficiently generated reactive oxygen species (ROS) under blue-light irradiation (420 ± 20 nm) and possessed a unimodal size distribution of 20–70 nm in diameter. The antimicrobial performance of the synthetized agents was examined against Gram-negative and Gram-positive bacteria. After a short-term incubation of microorganisms with nanomaterials (at 1 g/L) and irradiation with blue-light at a dose of 10 J/cm2, 2–3 logs of Escherichia coli, and 3–4 logs of Staphylococcus aureus were inactivated. A further decrease in bacteria viability was observed after potentiation photodynamic inactivation (PDI), either by H2O2 or KI, resulting in complete microorganism eradication even when using low material concentration (from 0.1 g/L). SEM analysis of bacteria morphology after each mode of PDI suggested different mechanisms of cellular disruption depending on the type of generated oxygen and/or iodide species. These data suggest that TiO2-based materials modified with sulfonated porphyrins are efficient photocatalysts that could be successfully used in biomedical strategies, most notably, photodynamic inactivation of microorganisms.

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