Detection of mislabeled commercial fishery by-products in the Philippines using DNA barcodes and its implications to food traceability and safety

Food Control ◽  
2013 ◽  
Vol 33 (1) ◽  
pp. 119-125 ◽  
Author(s):  
Benedict A. Maralit ◽  
Roselyn D. Aguila ◽  
Minerva Fatimae H. Ventolero ◽  
Sweedy Kay L. Perez ◽  
Demian A. Willette ◽  
...  
2005 ◽  
Vol 68 (1) ◽  
pp. 120-125 ◽  
Author(s):  
ANTHONY C. SALES ◽  
TAKUMI YOSHIZAWA

Mold counts and Aspergillus section Flavi populations in rice and its by-products from the Philippines were examined. The average mold counts of rough rice, brown rice, and locally produced polished rice were 4.1 × 103, 1.0 × 103, and 1.1 × 103 CFU/g, respectively. Average Aspergillus section Flavi counts of the same samples were 3.0 × 102, 1.1 × 102, and 2.6 × 102 CFU/g, respectively. Twenty-seven percent of mold isolates from rough rice, polished rice, and brown rice were section Flavi spp., 31% of which were toxigenic. No section Flavi isolates were obtained from imported rice samples from Thailand and Vietnam. Aspergillus section Flavi was also isolated from rice hull, rice bran, and settled dust from rice milling operations. Toxigenic isolates of both Aspergillus flavus and Aspergillus parasiticus were present in at least one sample of each type of rice and rice by-product except settled dust. Aflatoxins produced in vitro by the isolates ranged from <1 μg/kg to 6,227 μg/kg. A. flavus isolates produced only B aflatoxins, whereas A. parasiticus isolates produced both B and G aflatoxins. Although total mold counts of Philippine rice and its by-products are within tolerable limits, the establishment of maximum limits in counts of potentially aflatoxigenic species in foods and feeds is important because the mere presence of toxin producers is considered a possible risk factor. The results of this research illustrate the need for strict monitoring of rice during both storage and marketing, especially in warm and humid seasons when infestation and consequent production of aflatoxins by Aspergillus section Flavi is expected.


Biofuels ◽  
2020 ◽  
pp. 1-11
Author(s):  
Alchris Woo Go ◽  
Ian Dominic F. Tabañag ◽  
Yi-Hsu Ju ◽  
Angelique T. Conag ◽  
Arjay S. Toledo ◽  
...  

Zootaxa ◽  
2018 ◽  
Vol 4514 (1) ◽  
pp. 145 ◽  
Author(s):  
IAN NIEL B. DELA CRUZ ◽  
OLGA M. NUÑEZA ◽  
CHUNG-PING LIN

The nymph of the perlid Neoperla obliqua Banks, 1913 from Mt. Malindang, Mindanao Island, the Philippines is described and associated with the male and female adults using DNA barcodes. Using pairwise CO1, the nymph was associated with 99.9 ± 0.14% interspecific similarity, while comparison between sexes generated a 0.2% intraspecific divergence between males and females of putative conspecifics. Additionally, the mature ova from female adults are described using scanning electron microscopy (SEM). The distinctive chorionic surface is rugose with longitudinal ridge-like pattern with a bare and flat collar. 


Author(s):  
S. P. Khedekar

Concrete is the premier construction material around the world and is most widely used in all types of construction works, including infrastructure, low and high-rise buildings, and domestic developments. It is a man-made product, essentially consisting of a mixture of cement, aggregates, water and admixture(s). Inert granular materials such as sand, crushed stone or gravel form the major part of the aggregates. Traditionally aggregates have been readily available at economic prices and of qualities to suit all purposes. But the continued extensive extraction use of aggregates from natural resources has been questioned because of the depletion of quality primary aggregates and greater awareness of environmental protection. In light of this, the non-availability of natural resources to future generations has also been realized. Different alternative waste materials and industrial by products such as fly ash, bottom ash, recycled aggregates, foundry sand, China clay sand, crumb rubber, glass were replaced with natural aggregate and investigated properties of the concretes. Apart from above mentioned waste materials and industrial by products, few studies identified that coconut shells, the agricultural by product can also be used as aggregate in concrete. According to a report, coconut is grown in more than 86 countries worldwide, with a total production of 54 billion nuts per annum. India occupies the premier position in the world with an annual production of 13 billion nuts, followed by Indonesia and the Philippines.


2020 ◽  
Author(s):  
Shing Hei Zhan ◽  
Tsai-Yin Hsieh ◽  
Lan-Wei Yeh ◽  
Ting-Chun Kuo ◽  
Shoichiro Suda ◽  
...  

ABSTRACTThe global aquarium trade can introduce alien freshwater invaders, potentially impacting local aquatic ecosystems and their biodiversity. The role of the aquarium trade in spreading freshwater red macroalgae that hitchhike on ornamental aquatic plants and animals is unassessed. We investigated this human-mediated phenomenon via a broad biodiversity survey and genetic analysis of freshwater red algae in the field and aquarium shops in East Asia. Using rbcL-based DNA barcoding, we surveyed 125 samples from 46 field sites and 88 samples from 53 aquarium shops (213 samples in total) mostly across Taiwan – a key hub in the global aquarium trade – as well as in Hong Kong, Okinawa (Japan), the Philippines, and Thailand. We augmented our rbcL sequences with GenBank rbcL sequences that represent 40 additional countries globally. We found 26 molecular operational taxonomic units (mOTUs) in Taiwan, some of which are cryptic. Phylogeographical analysis revealed three potential introduced mOTUs in Taiwan, which exhibit no local genetic variation in Taiwan and are distributed across continents. Also, we posit that some presumably endangered freshwater red algae may be preserved in aquaria, an unintentional ex situ conservation site for these organisms that are vulnerable to water pollution from anthropogenic disturbances. Collectively, these data suggest that freshwater red algae have been hitchhiking and dispersed via the aquarium trade, an important and overlooked mechanism of introduction of these organisms across the globe.


Foods ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 249 ◽  
Author(s):  
Mark Balendres ◽  
Petr Karlovsky ◽  
Christian Cumagun

The tropical, warm, and humid conditions that are favorable to the growth and development of mycotoxigenic fungi put the Philippines at a high risk of mycotoxin contamination. To date, seven mycotoxigenic Aspergillus species, four Fusarium species, and one Penicillium species have been isolated from various agricultural crop commodities in the country. There are five mycotoxin groups (aflatoxin, fumonisin, ochratoxin, nivalenol, and zearalenone) that have been detected in both the raw form and the by-products of major crops grown in the country. Since the first scientific report of aflatoxin contamination in the Philippines in 1972, new information has been generated on mycotoxins and mycotoxigenic fungi, but little has been known of other mycotoxins until the last two decades. Further, despite the increase in the understanding of mycotoxigenic fungi and mycotoxins in the country, very limited knowledge exists on practices and measures that control both the fungi and the toxins. This paper reviews the current literature on mycotoxigenic fungi and mycotoxins in the Philippines with emphasis on the last two decades and on other mycotoxins.


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