Prevalence of enterotoxin genes and antibacterial susceptibility pattern of pathogenic bacteria isolated from traditionally preserved fish products of Sikkim, India

Food Control ◽  
2021 ◽  
Vol 125 ◽  
pp. 108009
Author(s):  
Meera Ongmu Bhutia ◽  
Namrata Thapa ◽  
Jyoti Prakash Tamang
Keyword(s):  
Pathogenic Bacteria ◽  
Susceptibility Pattern ◽  
Fish Products ◽  
Enterotoxin Genes ◽  
Antibacterial Susceptibility

Antibiotic susceptibility pattern against pathogenic bacteria causing Dental Caries

2011 ◽  
Vol 1 (1) ◽  
pp. 31-35
Author(s):  
Deepak Dwivedi ◽  
Tejram Kushwah ◽  
Mukesh Kushwah ◽  
Vinod Singh
Keyword(s):  
Dental Caries ◽  
Antibiotic Susceptibility ◽  
Pathogenic Bacteria ◽  
Resistant Bacteria ◽  
Susceptibility Pattern ◽  
Antibiotic Resistant ◽  
Antibiotic Susceptibility Pattern ◽  
Penicillin V ◽  
Antibiotics Susceptibility ◽  
Susceptibility Tests

Antibiotics to treat dental caries infection are routinely prescribed which led to the increased resistance against bacteria. The purpose of this investigation was to perform antibiotic susceptibility tests on a panel of pathogenic bacteria isolated from dental caries infection. Bacteria were isolated from caries site of patients and identified at the species level. Each of 150 species of bacteria was tested for antibiotics susceptibility to a five antibiotics using Etest. The antibiotics used were Amoxicillin, Cloxocillin, Erythromycin, Tetracycline and Penicillin‐V. The obtained resistance percentage for each antibiotic were Penicillin V: 72/150 (48%), Tetracycline: 99/150 (66%), Amoxicillin: 135/150 (90%), Cloxocillin: 117/150 (78%), and Erythromycin: 90/150 (60%) (Table 1). In case of combinatorial antibiotic exposure, the resistance percentage of Penicillin V/Amoxicillin and Amoxicillin/ Erythromycin was 39/150 (26%), and 45/150 (30%) respectively. The study has well demonstrated the clinical picture of antibiotic resistance and susceptibility pattern of bacteria causing dental caries. The obtained comprehensive data will allow investigating the spatial distribution of pathogenic, antibiotic resistant bacteria among dental caries patients which further may help into development of novel diagnostic and treatment approaches for the same.

VERIFICATION OF THE METHOD OF ISOLATION OF VIBRIO PARAHAEMOLYTICUS FROM SEA FISH AND FISH PRODUCTS

2019 ◽  
Vol 1 (2) ◽  
pp. 138-143
Author(s):  
I. M. Nityaga ◽  
◽  
B. V. Usha ◽  
O. V. Prostova ◽  
◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
Marine Fish ◽  
Vibrio Parahaemolyticus ◽  
Pathogenic Bacteria ◽  
Animal Feed ◽  
Fish Products ◽  
Gram Negative ◽  
Retail Chains ◽  
Sea Fish ◽  
Vibrio Spp

Analysis of microbiological safety of fish and fish products involves the assessment of the presence of a number of pathogenic bacteria in these products. One of these important bacteria is Vibrio parahaemolyticus – a gram-negative halophilic bacilus found in warm sea waters. The aim of this work was to verify the method of isolation of Vibrio parahaemolyticus from marine fish and fish products, as well as to study the presence of Vibrio samples of fish products from large retail chains. Verification of the method of detection of parahemolytic Vibrio from fish and fish products was carried out in accordance with GOST ISO/TS 21872-2-2013 "Microbiology of food and animal feed. Horizontal method for detection of potentially enteropathogenic Vibrio spp. Part 2. "Detection of bacteria species differs from Vibrio parahaemolyticus and Vibrio cholerae". As a result of the performed studies, the reliability of this method of detection of Vibrio parahaemolyticus on artificially contaminated samples was established. During monitoring fish products, parahemolytic vibrio was not detected.

scholarly journals Prevalence of Extended Spectrumβ-Lactamase and Antimicrobial Susceptibility Pattern of Clinical Isolates ofPseudomonasfrom Patients of Khyber Pakhtunkhwa, Pakistan

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Manzoor Ahmad ◽  
Mukhtiar Hassan ◽  
Anwar Khalid ◽  
Imran Tariq ◽  
Muhammad Hassham Hassan Bin Asad ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Pathogenic Bacteria ◽  
Tertiary Care ◽  
Diffusion Method ◽  
Susceptibility Pattern ◽  
Esbl Production ◽  
Antibiotic Resistant ◽  
Antimicrobial Susceptibility Pattern ◽  
Diffusion Technique ◽  
Sensitivity Rate

Majority of gram negative pathogenic bacteria are responsible for extended spectrumβ-lactamases (ESBLs) production, which show resistance to some newer generation of antibiotics. The study was aimed at evaluating the prevalence of ESBL and antibiotic susceptibility pattern ofPseudomonasisolates collected during 2010 to 2014 from tertiary care hospitals of Peshawar, Pakistan. Out of 3450 samples, 334Pseudomonasspp. isolates comprised of 232 indoor and 102 outdoor patients were obtained from different specimens and their susceptibility pattern was determined against 20 antibiotics. Antimicrobial susceptibility testing was carried out using the Kirby-Bauer agar diffusion method and ESBL production was detected by Synergy Disc Diffusion technique. The mean age group of the patients was 29.9 + 9.15 years. Meronem showed best activity (91.02%) from class carbapenem,β-lactam andβ-lactamase inhibitors exhibited 69.16% activity, and doxycycline had a diminished activity (10.18%) toPseudomonasspp. Outdoor isolates were more resistant than the indoor and during the course of the study the sensitivity rate of antibiotics was gradually reducing. ESBL production was observed in 44.32% while the remaining was non-ESBL. The moderate active antibiotics were amikacin (50.7%), SCF (51.4%), TZP (52.7%), and MXF (54.1%) among ESBL producing isolates. Lack of antibiotic policy, irrational uses (3GCs particularly), and the emergence of antibiotic resistant organisms in hospitals may be causes of high antibiotic resistance.

scholarly journals pathogenic bacteria, fish, DNA molecular, PCR

2010 ◽  
Vol 5 (2) ◽  
pp. 67 ◽  
Author(s):  
Dwiyitno Dwiyitno
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Clostridium Botulinum ◽  
Pathogenic Bacteria ◽  
Classical Method ◽  
Chain Reaction ◽  
Fish Products ◽  
Analytical Protocol ◽  
Seafood Products ◽  
Polymerase Chain

Food infection and intoxication of fish products are common due to consumption of foodexposed by either pathogenic or toxin promoter bacteria. A number of pathogenic bacteria havebeen identified related to fish and seafood such as Vibrio parahaemolyticusand other Vibrios,Escherichia coli, Aeromonas spp., Salmonellaspp., Staphylococcus aureus, Listeriamonocytogenes, Clostridium botulinum, C. perfringens, and Shigellaspp. However, isolationand identification of pathogenic bacteria from fish and seafood are often difficult due to the highnumber of contaminating and indigenous bacteria, while numbers of pathogenic bacteria arerelatively low. In the last 3 decades, several molecular methods have been developed and adoptedas official standard to supplement classical method, such as polymerase chain reaction (PCR)and its applications. Besides its limitation, molecular based identification offers advantages asroutine or screening analytical protocol for pathogenic bacteria in fish and seafood products.

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