Use of a portable near-infrared tool for rapid on-site inspection of freezing and hydrogen peroxide treatment of cuttlefish (Sepia officinalis).

Food Control ◽  
2021 ◽  
pp. 108524
Author(s):  
Sarah Currò ◽  
Luca Fasolato ◽  
Lorenzo Serva ◽  
Luciano Boffo ◽  
Jacopo Carlo Ferlito ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Near Infrared ◽  
Sepia Officinalis ◽  
Hydrogen Peroxide Treatment ◽  
On Site Inspection

Fluorescent proteins for in vivo imaging, where's the biliverdin?

2020 ◽  
Vol 48 (6) ◽  
pp. 2657-2667
Author(s):  
Felipe Montecinos-Franjola ◽  
John Y. Lin ◽  
Erik A. Rodriguez
Keyword(s):  
Hydrogen Peroxide ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Fluorescent Imaging ◽  
Infrared Light ◽  
Red Fluorescent Protein ◽  
Color Palette

Noninvasive fluorescent imaging requires far-red and near-infrared fluorescent proteins for deeper imaging. Near-infrared light penetrates biological tissue with blood vessels due to low absorbance, scattering, and reflection of light and has a greater signal-to-noise due to less autofluorescence. Far-red and near-infrared fluorescent proteins absorb light >600 nm to expand the color palette for imaging multiple biosensors and noninvasive in vivo imaging. The ideal fluorescent proteins are bright, photobleach minimally, express well in the desired cells, do not oligomerize, and generate or incorporate exogenous fluorophores efficiently. Coral-derived red fluorescent proteins require oxygen for fluorophore formation and release two hydrogen peroxide molecules. New fluorescent proteins based on phytochrome and phycobiliproteins use biliverdin IXα as fluorophores, do not require oxygen for maturation to image anaerobic organisms and tumor core, and do not generate hydrogen peroxide. The small Ultra-Red Fluorescent Protein (smURFP) was evolved from a cyanobacterial phycobiliprotein to covalently attach biliverdin as an exogenous fluorophore. The small Ultra-Red Fluorescent Protein is biophysically as bright as the enhanced green fluorescent protein, is exceptionally photostable, used for biosensor development, and visible in living mice. Novel applications of smURFP include in vitro protein diagnostics with attomolar (10−18 M) sensitivity, encapsulation in viral particles, and fluorescent protein nanoparticles. However, the availability of biliverdin limits the fluorescence of biliverdin-attaching fluorescent proteins; hence, extra biliverdin is needed to enhance brightness. New methods for improved biliverdin bioavailability are necessary to develop improved bright far-red and near-infrared fluorescent proteins for noninvasive imaging in vivo.

An in vitro senescence model of gingival epithelial cell induced by hydrogen peroxide treatment

Odontology ◽  
2021 ◽  
Author(s):  
Sarita Giri ◽  
Ayuko Takada ◽  
Durga Paudel ◽  
Koki Yoshida ◽  
Masae Furukawa ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Epithelial Cell ◽  
Gingival Epithelial Cell ◽  
Hydrogen Peroxide Treatment

Hydrogen Peroxide Treatment of Muscle Fibres Inhibits the Formation of Myosin Cross-Bridges

2018 ◽  
Vol 166 (2) ◽  
pp. 183-187 ◽  
Author(s):  
P. V. Kochubei ◽  
G. V. Kopylova ◽  
D. V. Shchepkin ◽  
S. Yu. Bershitskii
Keyword(s):  
Hydrogen Peroxide ◽  
Muscle Fibres ◽  
Hydrogen Peroxide Treatment ◽  
Cross Bridges

A novel colorimetric and near-infrared fluorescent probe for hydrogen peroxide imaging in vitro and in vivo

RSC Advances ◽  
2015 ◽  
Vol 5 (104) ◽  
pp. 85957-85963 ◽  
Author(s):  
Peng Wang ◽  
Ke Wang ◽  
Dan Chen ◽  
Yibo Mao ◽  
Yueqing Gu
Keyword(s):  
Hydrogen Peroxide ◽  
Fluorescent Probe ◽  
Near Infrared

A novel NIR fluorescent probe (DCM-B2) based on dicyanomethylene-4H-pyran was synthesized for the detection of H2O2.

Sign in / Sign up

Export Citation Format

Share Document