An in vitro senescence model of gingival epithelial cell induced by hydrogen peroxide treatment

Odontology ◽  
2021 ◽  
Author(s):  
Sarita Giri ◽  
Ayuko Takada ◽  
Durga Paudel ◽  
Koki Yoshida ◽  
Masae Furukawa ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Epithelial Cell ◽  
Gingival Epithelial Cell ◽  
Hydrogen Peroxide Treatment

In vitro modulation of human gingival epithelial cell attachment and migration by minocycline-HCI

2010 ◽  
Vol 33 (3) ◽  
pp. 377-385
Author(s):  
A. Vanheusden ◽  
B. Nusgens ◽  
G. Goffinet ◽  
S. Zahedi ◽  
C. M. Lapidre ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Attachment ◽  
Gingival Epithelial Cell ◽  
And Migration

scholarly journals Intraosseous transcutaneous amputation prostheses versus dental implants: A comparison between keratinocyte and gingival epithelial cell adhesion in vitro

2015 ◽  
Vol 29 ◽  
pp. 237-249 ◽  
Author(s):  
CJ Pendegrass ◽  
◽  
HT Lancashire ◽  
C Fontaine ◽  
G Chan ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Epithelial Cell ◽  
Dental Implants ◽  
Gingival Epithelial Cell

In vitro modulation of human gingival epithelial cell attachment and migration by minocycline-HCI

1998 ◽  
Vol 33 (6) ◽  
pp. 377-385 ◽  
Author(s):  
A. Vanheusden ◽  
B. Nusgens ◽  
G. Goffinet ◽  
S. Zahedi ◽  
C. M. Lapiere ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Attachment ◽  
Gingival Epithelial Cell ◽  
And Migration

scholarly journals Stabilization of Urokinase and Urokinase Receptor mRNAs by HuR Is Linked to Its Cytoplasmic Accumulation Induced by Activated Mitogen-Activated Protein Kinase-Activated Protein Kinase 2

2003 ◽  
Vol 23 (20) ◽  
pp. 7177-7188 ◽  
Author(s):  
Hoanh Tran ◽  
Fabienne Maurer ◽  
Yoshikuni Nagamine
Keyword(s):  
Hydrogen Peroxide ◽  
Protein Kinase ◽  
Active Form ◽  
Dominant Negative ◽  
Mitogen Activated Protein Kinase ◽  
Hydrogen Peroxide Treatment ◽  
Mitogen Activated Protein ◽  
Cytoplasmic Accumulation ◽  
In Cells

ABSTRACT The mRNAs of urokinase plasminogen activator (uPA) and its receptor, uPAR, contain instability-determining AU-rich elements (AREs) in their 3′ untranslated regions. The cellular proteins binding to these RNA sequences (AREuPA/uPAR) are not known. We show here that the mRNA-stabilizing factor HuR functionally interacts with these sequences. HuR stabilized an AREuPA-containing RNA substrate in vitro and stabilized in HeLa Tet-off cells both endogenous uPA and uPAR mRNAs and a β-globin reporter mRNA containing the AREuPA. RNAi-mediated depletion of HuR in BT-549 and MDA-MB-231 cells significantly reduced the steady-state levels of endogenous uPA and uPAR mRNAs. Furthermore, we show that a constitutively active form of mitogen-activated protein kinase-activated protein kinase 2 (MK2), MK2-EE, has an ARE-mRNA-stabilizing effect that correlates with its ability to enhance the cytoplasmic accumulation of endogenous HuR, but not in cells cotransfected with a dominant negative version of MK2, MK2-K76R. These effects were mimicked by hydrogen peroxide treatment (oxidative stress), which resulted in the phosphorylation of endogenous MK2. In addition, hydrogen peroxide treatment enhanced the cytoplasmic binding of HuR to the AREuPA, which was abrogated in cells transfected with MK2-K76R. These results indicate a role for HuR and MK2 in regulating the expression of uPA and uPAR genes at the posttranscriptional level.

In Vitro Stimulation of Human Gingival Epithelial Cell Attachment to Dentin by Surface Conditioning

1999 ◽  
Vol 70 (6) ◽  
pp. 594-603 ◽  
Author(s):  
Alain J. Vanheusden ◽  
Gérhard Goffinet ◽  
Sharam Zahedi ◽  
Betty Nusgens ◽  
Charles M. Lapiére ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Attachment ◽  
Surface Conditioning ◽  
Gingival Epithelial Cell ◽  
Stimulation Of

In vitro effects of hydrogen peroxide on ALF expression in male mouse germ cells

2014 ◽  
Author(s):  
Khaled S A Habas ◽  
Diana Anderson ◽  
Martin H Brinkworth
Keyword(s):  
Hydrogen Peroxide ◽  
Germ Cells ◽  
Male Mouse ◽  
In Vitro Effects

Met -/- kidneys express epithelial cells that chemotax and form tubules in response to EGF receptor ligands

1997 ◽  
Vol 272 (2) ◽  
pp. F222-F228
Author(s):  
C. Kjelsberg ◽  
H. Sakurai ◽  
K. Spokes ◽  
C. Birchmeier ◽  
I. Drummond ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epithelial Cell ◽  
Egf Receptor ◽  
Transforming Growth Factor ◽  
Growth Factor Receptor ◽  
Receptor Ligands ◽  
Tubule Formation ◽  
Human Papillomavirus 16 ◽  
Immortalized Cells

The growth factor/receptor combination of hepatocyte growth factor (HGF)/c-met has been postulated to be critical for mesenchymal-to-epithelial conversion and tubule formation in the developing kidney. We therefore isolated and immortalized cells from embryonic kidneys of met -/- transgenic mice to determine whether these cells were epithelial and able to chemotax and form tubules in vitro. The cells were immortalized with retrovirus expressing human papillomavirus 16 (HPV 16) E6/E7 genes. Two rapidly dividing clones were isolated and found to express the epithelial cell markers cytokeratin, zonula occludens-1, and E-cadherin but not to express the fibroblast marker vimentin. The met -/- cells were able to chemotax in response to epidermal growth factor and transforming growth factor-alpha (TGF-alpha) and form tubules in vitro in response to TGF-alpha but not HGF. These experiments suggest that the HGF/c-met axis is not essential for epithelial cell development in the embryonic kidney and demonstrate that other growth factors are capable of supporting early tubulogenesis.

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