An in vitro senescence model of gingival epithelial cell induced by hydrogen peroxide treatment

ABSTRACT The mRNAs of urokinase plasminogen activator (uPA) and its receptor, uPAR, contain instability-determining AU-rich elements (AREs) in their 3′ untranslated regions. The cellular proteins binding to these RNA sequences (AREuPA/uPAR) are not known. We show here that the mRNA-stabilizing factor HuR functionally interacts with these sequences. HuR stabilized an AREuPA-containing RNA substrate in vitro and stabilized in HeLa Tet-off cells both endogenous uPA and uPAR mRNAs and a β-globin reporter mRNA containing the AREuPA. RNAi-mediated depletion of HuR in BT-549 and MDA-MB-231 cells significantly reduced the steady-state levels of endogenous uPA and uPAR mRNAs. Furthermore, we show that a constitutively active form of mitogen-activated protein kinase-activated protein kinase 2 (MK2), MK2-EE, has an ARE-mRNA-stabilizing effect that correlates with its ability to enhance the cytoplasmic accumulation of endogenous HuR, but not in cells cotransfected with a dominant negative version of MK2, MK2-K76R. These effects were mimicked by hydrogen peroxide treatment (oxidative stress), which resulted in the phosphorylation of endogenous MK2. In addition, hydrogen peroxide treatment enhanced the cytoplasmic binding of HuR to the AREuPA, which was abrogated in cells transfected with MK2-K76R. These results indicate a role for HuR and MK2 in regulating the expression of uPA and uPAR genes at the posttranscriptional level.

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In Vitro Stimulation of Human Gingival Epithelial Cell Attachment to Dentin by Surface Conditioning

Journal of Periodontology ◽

10.1902/jop.1999.70.6.594 ◽

1999 ◽

Vol 70 (6) ◽

pp. 594-603 ◽

Cited By ~ 11

Author(s):

Alain J. Vanheusden ◽

Gérhard Goffinet ◽

Sharam Zahedi ◽

Betty Nusgens ◽

Charles M. Lapiére ◽

...

Keyword(s):

Epithelial Cell ◽

Cell Attachment ◽

Surface Conditioning ◽

Gingival Epithelial Cell ◽

Stimulation Of

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The novel histamine H2 receptor antagonist FRG-8813 prevents delay of wound repair induced by hydrogen peroxide in a rabbit gastric epithelial cell system

Journal of Gastroenterology and Hepatology ◽

10.1046/j.1440-1746.1998.01735.x ◽

1998 ◽

Vol 13 (11-s4) ◽

pp. S209-S213

Author(s):

NOBUHIRO SATO ◽

SUMIO WATANABE ◽

XIAN-EN WANG ◽

TARO OSADA ◽

HIROSHI TANAKA ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Epithelial Cell ◽

Receptor Antagonist ◽

Wound Repair ◽

Cell System ◽

The Novel ◽

H2 Receptor Antagonist ◽

Histamine H2 Receptor ◽

H2 Receptor ◽

Histamine H2 Receptor Antagonist

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In vitro effects of hydrogen peroxide on ALF expression in male mouse germ cells

Reproduction Abstracts ◽

10.1530/repabs.1.p260 ◽

2014 ◽

Author(s):

Khaled S A Habas ◽

Diana Anderson ◽

Martin H Brinkworth

Keyword(s):

Hydrogen Peroxide ◽

Germ Cells ◽

Male Mouse ◽

In Vitro Effects

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Met -/- kidneys express epithelial cells that chemotax and form tubules in response to EGF receptor ligands

AJP Renal Physiology ◽

10.1152/ajprenal.1997.272.2.f222 ◽

1997 ◽

Vol 272 (2) ◽

pp. F222-F228

Author(s):

C. Kjelsberg ◽

H. Sakurai ◽

K. Spokes ◽

C. Birchmeier ◽

I. Drummond ◽

...

Keyword(s):

Growth Factor ◽

Epithelial Cell ◽

Egf Receptor ◽

Transforming Growth Factor ◽

Growth Factor Receptor ◽

Receptor Ligands ◽

Tubule Formation ◽

Human Papillomavirus 16 ◽

Immortalized Cells

The growth factor/receptor combination of hepatocyte growth factor (HGF)/c-met has been postulated to be critical for mesenchymal-to-epithelial conversion and tubule formation in the developing kidney. We therefore isolated and immortalized cells from embryonic kidneys of met -/- transgenic mice to determine whether these cells were epithelial and able to chemotax and form tubules in vitro. The cells were immortalized with retrovirus expressing human papillomavirus 16 (HPV 16) E6/E7 genes. Two rapidly dividing clones were isolated and found to express the epithelial cell markers cytokeratin, zonula occludens-1, and E-cadherin but not to express the fibroblast marker vimentin. The met -/- cells were able to chemotax in response to epidermal growth factor and transforming growth factor-alpha (TGF-alpha) and form tubules in vitro in response to TGF-alpha but not HGF. These experiments suggest that the HGF/c-met axis is not essential for epithelial cell development in the embryonic kidney and demonstrate that other growth factors are capable of supporting early tubulogenesis.

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