scholarly journals Food emulsifiers based on milk fat globule membranes and their interactions with calcium and casein phosphoproteins

2019 ◽  
Vol 94 ◽  
pp. 30-37 ◽  
Author(s):  
Annamari Jukkola ◽  
Riitta Partanen ◽  
Wenchao Xiang ◽  
Antti Heino ◽  
Orlando J. Rojas
Keyword(s):  
Milk Fat ◽  
Milk Fat Globule ◽  
Food Emulsifiers ◽  
Fat Globule ◽  
Milk Fat Globule Membranes

scholarly journals Isolation and characterization of two individual glycoprotein components from human milk-fat-globule membranes

1982 ◽  
Vol 207 (1) ◽  
pp. 37-41 ◽  
Author(s):  
A Imam ◽  
D J Laurence ◽  
A M Neville
Keyword(s):  
Human Milk ◽  
Milk Fat ◽  
Sodium Dodecyl ◽  
Membrane Glycoproteins ◽  
Lectin Affinity Chromatography ◽  
Isolation And Characterization ◽  
Milk Fat Globule ◽  
Fat Globule ◽  
Milk Fat Globule Membranes ◽  
Human Milk Fat

Two individual glycoprotein components from human milk-fat-globule membranes (MFGM) has been purified by selectively extracting the membrane glycoproteins followed by lectin affinity chromatography and gel filtration on Sephadex G-200 in the presence of protein-disaggregating agents. The purified glycoprotein components, termed ‘epithelial-membrane glycoprotein’ (EMGP-155 and EMGP-39) have estimated molecular weights of 155 000 and 39 000 respectively, and yield a single band under reducing conditions on sodium dodecyl sulphate/polyacrylamide gel. EMGP-155 and EMGP-39 contain 21.0% and 7.0% carbohydrate by weight, with fucose (13.5%, 12.4%), mannose (3.7%, 6.2%), galactose (28.5%, 22.6%), N-acetylglucosamine (17.8%, 7.4%) and sialic acid (36.4%, 51.4%) of the carbohydrate moiety respectively. For both the glycoprotein components, aspartic and glutamic acid and serine are the major amino acid residues.

Reactions of Monoclonal Antibodies against Human Milk Fat Globule Membranes with Embryonal Tissue

1984 ◽  
pp. 1021-1024 ◽  
Author(s):  
U. KOLDOVSKY ◽  
U. WARGALLA ◽  
J. HILKENS ◽  
J. TAYLOR-PAPADIMITRIOU ◽  
PH. HAGEMANN ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Human Milk ◽  
Milk Fat ◽  
Milk Fat Globule ◽  
Fat Globule ◽  
Milk Fat Globule Membranes ◽  
Human Milk Fat

scholarly journals BIOCHEMICAL AND MORPHOLOGICAL COMPARISON OF PLASMA MEMBRANE AND MILK FAT GLOBULE MEMBRANE FROM BOVINE MAMMARY GLAND

1970 ◽  
Vol 44 (1) ◽  
pp. 80-93 ◽  
Author(s):  
T. W. Keenan ◽  
D. James Morré ◽  
Diane E. Olson ◽  
W. N. Yunghans ◽  
Stuart Patton
Keyword(s):  
Fatty Acid Composition ◽  
Plasma Membrane ◽  
Fatty Acid ◽  
Acid Composition ◽  
Milk Fat ◽  
Major Protein ◽  
Thin Sections ◽  
Milk Fat Globule ◽  
Fat Globule ◽  
Milk Fat Globule Membranes

Purified plasma membrane fractions from lactating bovine mammary glands and membranes of milk fat globules from the same source were similar in distribution and fatty acid composition of phospholipids. The sphingomyelin content of the phospholipid fraction of both membranes was higher than in these fractions from other cell components, ß-carotene, a constituent characteristic of milk fat, was present in the lipid fraction of the plasma membrane. Cholesterol esters of plasma membrane were similar in fatty acid composition to those of milk fat globule membranes. Disc electrophoresis of either membrane preparation on polyacrylamide gels revealed a single major protein component characteristic of plasma membrane from other sources. Distinct morphological differences between plasma membrane and milk fat globule membranes were observed in both thin sections and in negatively stained material. Plasma membrane was vesicular in appearance while milk fat globule membranes had a platelike aspect. These observations are consistent with derivation of fat globule membrane from plasma membrane accompanied by structural rearrangement of membrane constituents.

Milk-fat globule membranes: chemical composition and phosphoesterase activities during lactation

1977 ◽  
Vol 44 (3) ◽  
pp. 483-493 ◽  
Author(s):  
Teresa Diaz-Mauriño ◽  
M. Nieto
Keyword(s):  
Amino Acid ◽  
Chemical Composition ◽  
Amino Acid Analysis ◽  
Milk Fat ◽  
Stage Of Lactation ◽  
Milk Fat Globule ◽  
Fat Globule ◽  
Milk Fat Globule Membranes ◽  
Carbohydrate Contents ◽  
Acid Phosphomonoesterase

SummaryThe morphology, protein and glycoprotein composition, amino-acid analysis, phospholipid and carbohydrate contents and 3 phosphoesterase activities of the milk-fat globule membranes were studied in 4 cows during their whole period of lactation. No differences were found in the morphology and the overall chemical composition. However, glycoprotein composition varied with the cow and, for each cow, with the stage of lactation. The differences were more marked for membranes obtained from colostrum. Acid phosphomonoesterase was practically unchanged during the whole period of lactation, while alkaline phosphomonoesterase and phosphodiesterase increased as lactation proceeded.

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