Comparative genomics and antibiotic resistance of Yersinia enterocolitica obtained from a pork production chain and human clinical cases in Brazil

2021 ◽  
pp. 110917
Author(s):  
Bruna Torres Furtado Martins ◽  
João Luiz de Meirelles ◽  
Wellington Pine Omori ◽  
Rafael R. de Oliveira ◽  
Ricardo Seiti Yamatogi ◽  
...  
2021 ◽  
Vol 854 (1) ◽  
pp. 012043
Author(s):  
Nedjeljko Karabasil ◽  
Tamara Boskovic ◽  
Dragan Vasilev ◽  
Nikola Betic ◽  
Mirjana Dimitrijevic

Abstract As pork and pork products represent an important part of the diet, the issue of pork safety and quality has become more prominent. Food safety concerns are shaping consumers’ attitudes toward safe food. The farm and meat sectors aim at producing healthy animals in a protected environment, which is a key point for food/meat safety. The most common biological hazards in the pork production chain are Salmonella spp., Yersinia enterocolitica, Trichinella spp. and Toxoplasma gondii. These hazards are not detectable by conventional meat inspection, and measures rely on prevention or reduction of contamination along the production chain.


2019 ◽  
Vol 310 ◽  
pp. 108310 ◽  
Author(s):  
Cibeli Viana ◽  
Mallu Jagnow Sereno ◽  
Kadigia Pegoraro ◽  
Ricardo Seiti Yamatogi ◽  
Douglas Ruben Call ◽  
...  

2018 ◽  
Vol 276 ◽  
pp. 5-9 ◽  
Author(s):  
Bruna Torres Furtado Martins ◽  
Clarisse Vieira Botelho ◽  
Danilo Augusto Lopes Silva ◽  
Frederico Germano Piscitelli Alvarenga Lanna ◽  
Juliana Libero Grossi ◽  
...  

2021 ◽  
Vol 94 ◽  
pp. 103660
Author(s):  
Bruna Torres Furtado Martins ◽  
Everton Cruz de Azevedo ◽  
Ricardo Seiti Yamatogi ◽  
Douglas Ruben Call ◽  
Luís Augusto Nero

2021 ◽  
Vol 4 (2) ◽  
pp. 23-26
Author(s):  
Isa Shu’aibu ◽  
Habiba Abdullahi ◽  
S. Hanna Kadum ◽  
A. Jabir Hamza ◽  
Y. Kabiru Mustapha ◽  
...  

In Africa, the use of poor and unhygienic methods for animal milking and milk processing that leads to spoilage of milk by microbes affects the production of milk and dairy products, especially in the small scale and local processing plants. This study was conducted to analyze the quality and safety of raw milk collected from six different towns in Kwami local government area of Gombe State, Nigeria. The samples were serially diluted using ten-fold dilution and used aliquot 1 ml to inoculate the appropriate media using pour plate technique. The total viable count for bacteria in CFU/ml on plate count agar (PCA) was highest in sample E from U/Anchau with an average of total viable count of 3.8x104 CFU/ml, followed by 3.0x104 CFU/ml in sample B (Dirri), then 2.8x104 CFU/ml in sample D from Burakosuma, 2.5x104 CFU/ml in sample F from Dun urji, 2.3x104 CFU/ml in sample C from Zanbe with least count from sample A at Bele as 1.8x104 CFU/ml. Five (5) bacterial species of public health importance were isolated and identified using biochemical tests namely; Enterobacter sp., Yersinia enterocolitica, Escherichia coli, Staphylococcus aureus and Salmonella sp.. Out of the organisms isolated, Enterobacter sp. had the highest occurrence of 93% (n=280), then Yersinia enterocolitica 90% (n=270), E. coli 70% (n=210), S. aureus 57% (n=170), and finally Salmonella sp. 23% (n=70). Based on the microbiological outcomes, preventive measures for milking and processing that focus on training of farmers and dairy employees for the improvement of the hygiene of local milk and dairy production chain should be defined.


2020 ◽  
Vol 17 (3) ◽  
pp. 194-201
Author(s):  
Cibeli Viana ◽  
Mallu Jagnow Sereno ◽  
Luciano dos Santos Bersot ◽  
Jalusa Deon Kich ◽  
Luís Augusto Nero

2018 ◽  
Vol 38 (3) ◽  
pp. 393-399 ◽  
Author(s):  
Débora D.S. Gobbi ◽  
Maria G. Spindola ◽  
Luisa Z. Moreno ◽  
Carlos E.C. Matajira ◽  
Maria G.X. Oliveira ◽  
...  

ABSTRACT: Arcobacter is an emerging zoonotic pathogen, and the major transmission routes to humans are the handling or consumption of contaminated raw/undercooked food products of animal origin, water and seafood. The isolation and identification of Arcobacter species are not routine in clinical laboratories; therefore, its true incidence in human infections may be underestimated. The present study aimed to isolate and characterize Arcobacter from carcasses and fecal samples collected at swine slaughterhouses and from meat markets in São Paulo State, Brazil. The isolates were identified using multiplex-PCR to differentiate the species and analyzed by single-enzyme amplified fragment length polymorphism (SE-AFLP). Arcobacter spp. were isolated from 73.0% of swine carcasses, 4% of fecal samples and 10% of pork samples. A. butzleri was the most prevalent species identified, followed by A. cryaerophilus. Interestingly, the carcasses presented higher frequency of A. butzleri isolation, whereas only A. cryaerophilus was isolated from fecal samples. SE-AFLP enabled the characterization of A. butzleri and A. cryaerophilus into 51 and 63 profiles, respectively. The great genetic heterogeneity observed for both species corroborates previous reports. This study confirms the necessity for a standard isolation protocol and the improvement of molecular tools to further elucidate Arcobacter epidemiology.


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