Algorithm for establishing the time of death of a dog based on temperature measurements in selected sites of the body during the early post-mortem period

2018 ◽  
Vol 289 ◽  
pp. 124-129
Author(s):  
P. Listos ◽  
M. Gryzinska ◽  
J. Batkowska ◽  
M. Grela ◽  
A. Jakubczak
2018 ◽  
Vol 74 (1) ◽  
pp. 33-38
Author(s):  
PIOTR LISTOS ◽  
MAGDALENA GRYZIŃSKA ◽  
JUSTYNA BATKOWSKA ◽  
MAŁGORZATA DYLEWSKA ◽  
KATARZYNA CZEPIEL-MIL

Precisely determined time of death is one of the most important pieces of information obtained during a post-mortem investigation. There are several traditional methods for determining time of death, the most important of which are evaluation of early post-mortem changes, such as the change in body temperature. The study was aimed at identifying insects collected from the body and establishing the time of death by observing the developmental cycle of the indicator species Calliphora vicina. The material for the main experiment was the carcass of a dog. The length of the developmental cycles of insects depends on ambient conditions, so studies should take as many criteria as possible into account. Succession of arthropods, as well as the species composition and indicator insects of particular stages of decomposition, is very similar in human and animal carcasses. The results obtained were considered with respect to the time that had passed since the death of the animal. Experiments on dog carcasses may in the future contribute to the development of research enabling determination of the time of death of animals, which is a subject of increasing interest in forensic veterinary medicine....


Author(s):  
Richard D.W. Hain ◽  
Satbir Singh Jassal

In helping children to have a ‘good death’, it is vitally important to understand the practical issues around death. Parents often have little or no understanding of this and will look to the health-care professional for guidance. When done well, the parents are left unaware of the complexities involved. However, when done badly, the parents can be left feeling very upset. Predicting the time of death for a child is notoriously difficult, and this issue is addressed by this chapter. A detailed overview of practicalities around the time of death is provided, with information on the correct procedure before death, certifying and registering a death, cremation, organ and tissue donation, and transporting and looking after the body. Further practicalities, including the post-mortem, the role of the coroner, and the role of the Child Death Overview Panel, are also covered.


2016 ◽  
Vol 154 (1) ◽  
pp. 87
Author(s):  
P. Listos ◽  
W. Łopuszyński ◽  
M. Gryzińska ◽  
J. Piórkowski ◽  
A. Śmiech ◽  
...  

1899 ◽  
Vol 45 (191) ◽  
pp. 758-760
Author(s):  
E. B. Whitcombe

The patient, thirty-nine years of age, was admitted into Birmingham Asylum in February, 1898. He was a porter, married, in fairly robust condition, and was a typical example, both mentally and physically, of general paralysis of the insane of somewhat short duration. He was stated to have been steady, of temperate habits, and had been in the army. For twelve years he served in India. No history of fevers or other illness. The disease progressed without any special features until January 14th of this year, when he was noticed to be worse; his breathing was a little rapid, and in consequence he was sent to the infirmary ward and was examined thoroughly by the assistant medical officer, who found nothing specially interesting, but ordered him to be put to bed and kept warm. This was about 3 o'clock in the afternoon. At 7 o'clock the same evening I was asked to see the patient (he had been examined at 5 o'clock by the nurse). I found the left leg from thigh to toe was double the size of the other leg, and nearly the whole surface of the leg was perfectly black, and there were numerous large bullæ the size of one's fist in different places along the leg. There was no special line of demarcation. At first sight it looked like an extreme case of local purpura, but after a careful examination I came to the conclusion that putrefaction had actually set in, and that the man was dying, and death took place an hour after I saw him. The most extraordinary part of this case occurred afterwards. I am accustomed to go and see a body before giving my certificate to the coroner. I saw this man between 10 and 11 on Sunday morning, he having died at 8 p.m. on Saturday. The body was double the former size; it was more like the body of a negro, the whole surface being in a black condition, and the bullæ had increased on the other parts of the body. The scrotum was distended to the size of a man's head, and the penis swelled and distorted. The case was the more extraordinary as the highest temperature recorded locally at the time was 52·8°, and the lowest 34°. I personally saw the coroner, and together we went through numerous works on jurisprudence, but we could find nothing to give us any idea as to the cause of this condition, and he very kindly and in scientific interests ordered an inquest. He sent Dr. Simon, Professor of Medical Jurisprudence in Mason College, to make the post-mortem examination. The results were practically nil, the whole body internally and externally being putrefied. The cause of death was very naturally put down to general paralysis, but as to any cause for this extremely rapid putrefaction we could arrive at no conclusion. The case is one of very great interest. I believe that the first idea that the nurse had in the infirmary was that this man must have been injured. Now there was the usual considerable difference between the appearance of an injury and this condition, which looked like purpura; but besides this the difficulty that occurred to my mind was as to the fixing of the time of death. Here was a body presenting the appearances which are usually recognised as those of three or four weeks' duration, and these had happened certainly within sixteen hours. From the point of view of jurisprudence it occurred to me that a murder might be committed, that the body might present these appearances, and that it would be a most serious matter for a medical man to give an opinion as to the time of death. We know that in hot countries this condition does occur, but we were in the middle of winter, and the condition arose from, so far as we could judge, no special cause whatever. There was some atheroma of the arteries, but otherwise we could distinguish nothing of importance at the post-mortem. It is to be regretted that no bacteriological examination was made.


1977 ◽  
Vol 17 (2) ◽  
pp. 112-122 ◽  
Author(s):  
Jørn Simonsen ◽  
Jorgen Voigt ◽  
Niels Jeppesen

In 20 cases with known times of death continuous post-mortem measurements of the temperature fall in brain, calf, liver, axilla and rectum of the bodies have been made, and, in addition, the environmental temperature has been recorded. The observations were not made under standardized conditions, and the clothing of the bodies was left untouched as far as possible. The measurements of the brain temperatures have given the greatest accuracy in determining the time of death; for temperatures above 25 °C the uncertainty was of the order of magnitude of ±2 1/2 hours, at lower temperatures greater. The other sites of measurement permitted less reliable estimates of the post-mortem time, but none of them were found to be appropriate beyond 20 hours after death. There is one factor which cannot be calculated. It is the temperature at the moment of death. All investigations show that it may vary enormously. In the present study the difference between the maximum and the minimum starting temperature ranges between 5 °C and 8 °C, dependent on the site of measurement. As the fall in temperature—irrespective of the site of measurement—during the first few hours post mortem is of the magnitude of 1 °C per hour, the above variation gives an inaccuracy which by far exceeds what can be achieved of greater accuracy by the aid of brain temperature measurements. For this reason the authors feel justified in concluding that the determination of the time of death will always be encumbered with great uncertainty, but that the most reliable estimate within the first 20 hours after death can be based upon the measurement of the brain temperature associated with an evaluation of the development of the signs of death. None of the other methods tested so far appears to have offered a greater reliability.


2020 ◽  
pp. 002580242093423 ◽  
Author(s):  
Covadonga Palacio ◽  
Rossella Gottardo ◽  
Vito Cirielli ◽  
Giacomo Musile ◽  
Yvane Agard ◽  
...  

Post-mortem changes of ions in the body fluids have been proposed as an objective tool for inferring the time of death. In particular, the post-mortem increase of potassium concentrations in the vitreous humour has gained great attention in the literature. On the other hand, ammonium, another ion released in post-mortem processes, has received much less attention, potentially due to unresolved analytical issues using current clinical chemistry methods. This paper presents an application of a new analytical approach based on capillary electrophoresis providing the simultaneous analysis of potassium and ammonium ions in the vitreous humour. In addition, to assess the consistency of the post-mortem increase of ammonium concentrations in the vitreous humour, the determination of this ion in the vitreous humour of the two eyes of the same body at the same post-mortem interval has been verified. Vitreous humour was collected from 33 medico-legal cases where the time of death was known exactly. Prior to analysis, all samples were diluted 1:20 with a 40 μg/mL solution of BaCl2 (internal standard). In the study of the variability of the ammonium concentration between the two eyes, no statistically significant differences were found, supporting the hypothesis of an even post-mortem increase of the ion concentrations in this particular biological fluid. Significant correlations of potassium and ammonium ions with the post-mortem interval were found, with r2 of 0.75 and 0.70, respectively.


Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 226
Author(s):  
Silvio De Luca ◽  
Emanuela Zanardi ◽  
Giovanni Loris Alborali ◽  
Adriana Ianieri ◽  
Sergio Ghidini

The assessment of swine welfare requires feasible, reliable, and reasonable indicators. On-farm evaluation of pig welfare can provide valuable information to veterinarians and farmers. However, such protocols can result expensive and time-consuming. With this regard, an interest in the appraisal of swine welfare at abattoir has grown over the recent years. In particular, the use of certain lesions collected directly from slaughtered animals to determine the welfare status of pigs has been evaluated by several authors. In the present review, the different methods developed to score lesions collected directly from the body and the viscera of animals slaughtered in European abattoirs (“abattoir-based measures”) are presented. The text specifically focuses on the methods currently available in the literature for the scoring of body, pluck and gastric lesions during post-mortem activities. Moreover, the strengths and weaknesses of abattoir-based measures schemes are discussed. To conclude, the future perspectives of the assessment of pig welfare at the slaughterhouse are described, appealing for a benchmarking system that can be systematically used by veterinarians and other professional figures involved in the process.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Raphael Severino Bonadio ◽  
Larissa Barbosa Nunes ◽  
Patricia Natália S. Moretti ◽  
Juliana Forte Mazzeu ◽  
Stefano Cagnin ◽  
...  

AbstractMost biological features that occur on the body after death were already deciphered by traditional medicine. However, the molecular mechanisms triggered in the cellular microenvironment are not fully comprehended yet. Previous studies reported gene expression alterations in the post-mortem condition, but little is known about how the environment could influence RNA degradation and transcriptional regulation. In this work, we analysed the transcriptome of mouse brain after death under three concealment simulations (air exposed, buried, and submerged). Our analyses identified 2,103 genes differentially expressed in all tested groups 48 h after death. Moreover, we identified 111 commonly upregulated and 497 commonly downregulated genes in mice from the concealment simulations. The gene functions shared by the individuals from the tested environments were associated with RNA homeostasis, inflammation, developmental processes, cell communication, cell proliferation, and lipid metabolism. Regarding the altered biological processes, we identified that the macroautophagy process was enriched in the upregulated genes and lipid metabolism was enriched in the downregulated genes. On the other hand, we also described a list of biomarkers associated with the submerged and buried groups, indicating that these environments can influence the post-mortem RNA abundance in its particular way.


1994 ◽  
Vol 165 (6) ◽  
pp. 787-791 ◽  
Author(s):  
Nedzara Jusic ◽  
Malcolm Lader

BackgroundThe relationship between antipsychotic drug treatment and sudden unexplained death remains unclear. The estimation of post-mortem blood drug concentrations should be helpful.MethodEight medico-legal cases were reviewed with respect to behaviour of patient, type and dosage of drug treatment, mode of death, post-mortem findings and drug concentrations.ResultsThe problems of evaluating such drug levels are discussed. Five of the eight patients had probably toxic concentrations of antipsychotic and/or antidepressants, which caused death, usually involving cardiac arrhythmias.ConclusionsIn cases of sudden unexpected death, a sample of blood from a peripheral vein should be obtained immediately death is pronounced or the body discovered, and sent for analysis. To minimise such fatalities, the patient should be monitored carefully, with ECG if feasible, and electrolyte balance checked. The drug regimen used should be kept simple and large doses of antipsychotics and/or antidepressants avoided wherever possible.


1978 ◽  
Vol 14 (3) ◽  
pp. 343-359
Author(s):  
Philip L. Quinn

Suppose that a person P1 dies some time during 1978. Many years later, the resurrection world, a perennial object of Christian concern, begins on the morning of the day of judgment. On its first morning there are in that world distinct persons, P2 and P3, each of whom is related in remarkably intimate ways to P1. You are to imagine that each of them satisfies each of the criteria or conditions necessary for identity with P1 to some extent, that both of them satisfy these conditions to exactly the same extent, and that every other denizen of the resurrection world satisfies each of these conditions to a lesser extent than P2 and P3 do. Thus, for example, philosophers often claim that bodily continuity is a necessary condition for personal identity. If it is, you might assume that the body P2 has on the morning of the day of judgment contains some of the same atoms the body of P11 contained when P1 died, and that P2's body on that day contains exactly n atoms from P1's body at the time of death just in case P3's body on that day contains exactly n atoms from P1's body at the time of death. Or, again, some philosophers hold that connectedness of memory is necessary for personal identity. If so, you are to suppose that on the morning of the day of judgment P3 seems to remember some of the events in the life of P1 having happened to him, and that P3 seems to remember a certain event in the life of P1 having happened to him just in case P2 seems to remember that very event in the life of P1 having happened to him. You are to fill in the details by adding complete parity between P2 and P3 with respect to similarity of DNA molecules, character traits and whatever else you deem relevant to personal identity. And, finally, you are to complete the story by imagining that P2 and P3 live very different sorts of lives in the resurrection world. To heighten the poignancy of the story, you might imagine that P2 enjoys forever after the beatitude promised to the blessed while P3 suffers the everlasting torments reserved for the damned.


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