scholarly journals Genetic characterization and determination of the number of individuals by molecular analysis in a prehistoric finding

2017 ◽  
Vol 6 ◽  
pp. e487-e489 ◽  
Author(s):  
Sara Palomo-Díez ◽  
Ángel Esparza-Arroyo ◽  
Cláudia Gomes ◽  
Olga Rickards ◽  
Javier Velasco-Vázquez ◽  
...  
Keyword(s):  
Molecular Analysis ◽  
Genetic Characterization ◽  
Number Of Individuals

scholarly journals Methods Applied for Measurement and Visualization of Changes in Biodiversity

2015 ◽  
Vol 21 (4) ◽  
pp. 593-604 ◽  
Author(s):  
Grzegorz Łagód ◽  
Mariola Chomczyńska ◽  
Agnieszka Montusiewicz ◽  
Jacek Malicki ◽  
David Stransky
Keyword(s):  
Statistical Significance ◽  
Shannon Index ◽  
Taxon Richness ◽  
Profile Method ◽  
Maximum Value ◽  
Structural Capacity ◽  
The Monte Carlo Method ◽  
Number Of Individuals ◽  
Biological Differences

Abstract The article presents the possible methods for determining biological or statistically significant differences between taxocenoses compared with respect to biodiversity. To obtain a complete description of biological differences between the compared hypothetical communities, the following indices were calculated: S (taxon richness), H (the Shannon index), Hmax (the maximum value of the Shannon index for the richness of taxa represented by the same number of individuals), Vd (a percentage value of covering the structural capacity of community, “evenness deficiency”), E (the MacArthur index - a taxon number (S) in a community for which the observed value of H equals Hmax), and Ps (a taxon richness shortage in percents). Moreover, a graphic profile method (Дд, Tj, and Lj profiles) was used for comparing the diversity of the communities. To obtain information about statistically significant differences in biodiversity between the analysed communities, rarefaction curves were applied. The curves are based on the null models and the Monte Carlo method. The rarefaction method resulted in determination of the statistical significance of the differences between taxon richness and Shannon's index values for the compared communities. The Vd and Ps indices and the profile method allowed concluding about the significance of the biological differences between taxocenoses, even when their values of Shannon's H indices were numerically similar.

scholarly journals Herbaceous periwinkle, Vinca herbacea Waldst. et Kit. 1799 (Apocynaceae), a new species of the Croatian flora

2013 ◽  
Vol 72 (2) ◽  
pp. 399-406
Author(s):  
János Csiky ◽  
Dragica Purger
Keyword(s):  
New Species ◽  
Small Extent ◽  
Critically Endangered ◽  
The South ◽  
Plant Identification ◽  
Data Deficient ◽  
Number Of Individuals ◽  
A New Species

Abstract - Populations of herbaceous periwinkle, Vinca herbacea Waldst. et Kit., were found on April 2007 on Bansko Hill (Baranja, Croatia), which lies on the south-western edge of the range of this Pontic-Pannonian species. Since V. herbacea was included neither in the handbooks for plant identification nor in the current Croatian Flora Database, a new key for the determination of Vinca L. species of Croatia is presented herein. The herbaceous periwinkle should be treated as a critically endangered (CR) species in Croatia, considering the low number of individuals and the small extent of its occurrence in extremely rare habitats at the margin of its distribution. New recordings of some very rare or »data deficient « (DD) taxa of Croatia are also presented here: Scorzonera hispanica L. and Inula germanica L.

Determination of HPRT mutant frequency and molecular analysis of T-lymphocyte mutants derived from coke-oven workers

1998 ◽  
Vol 412 (2) ◽  
pp. 177-186 ◽  
Author(s):  
Nicola Zanesi ◽  
Maddalena Mognato ◽  
Massimo Pizzato ◽  
Cristina Viezzer ◽  
Giovanni Ferri ◽  
...  
Keyword(s):  
Molecular Analysis ◽  
T Lymphocyte ◽  
Coke Oven ◽  
Coke Oven Workers ◽  
Mutant Frequency

scholarly journals Building a method for the study of the Mesolithic Neolithic transition in Portugal

2004 ◽  
Vol 31 ◽  
pp. 88-111 ◽  
Author(s):  
Mary Jackes ◽  
Christopher Meiklejohn
Keyword(s):  
Age Assessment ◽  
Neolithic Transition ◽  
Agricultural Transition ◽  
Regional Population ◽  
Population Continuity ◽  
Historical Issues ◽  
Number Of Individuals ◽  
Skeletal Samples ◽  
Late Mesolithic

This paper focuses on the agricultural transition in Portugal and on demography across this transition, concentrating on two key skeletal samples, the Mesolithic shell midden of Cabeco da Arruda and the Neolithic burial cave of Casa da Moura. It extends our previous work on the demography of the transition and the methodology surrounding its determination. We explain our method for determination of the number of individuals in samples where whole skeletons cannot be used. We then concentrate on the estimation of fertility, placing it within limits of biological feasibility, sample inadequacies, and vagaries of age assessment. From our analysis, which includes an examination of historical issues with the sites, we argue for regional population continuity between 8000 and 6000 cal BP, and suggest that Neolithic life-ways slowly intensified, founded on important elements deriving from the late Mesolithic, with changes that included increased fertility through shortening of the birth interval

Microsatellite instability diagnosis using the fully automated Idylla platform: feasibility study of an in-house rapid molecular testing ancillary to immunohistochemistry in pathology laboratories

2019 ◽  
Vol 72 (12) ◽  
pp. 830-835 ◽  
Author(s):  
Laura Samaison ◽  
Mélanie Grall ◽  
Frédéric Staroz ◽  
Arnaud Uguen
Keyword(s):  
Microsatellite Instability ◽  
Feasibility Study ◽  
Molecular Analysis ◽  
Diagnostic Tool ◽  
Microsatellite Analysis ◽  
Molecular Testing ◽  
Molecular Analyses ◽  
Microsatellite Stability ◽  
Rapid Treatment

AimTo study the performances of the Idylla MSI Assay in the diagnosis of microsatellite instability (MSI) or microsatellite stability (MSS).MethodsWe selected 12 tumour samples previously tested for MSI focusing on cases with discrepant results between MLH1, PMS2, MSH2 and MSH6 immunohistochemistry and microsatellite molecular analyses (five cases) or doubtful immunohistochemistry (two cases). Idylla MSI Assay was compared with retrospective immunohistochemistry and molecular results.ResultsIdylla MSI Assay showed an almost perfect concordance with microsatellite analysis results previously obtained (only one case with not fully conclusive analysis due to sample exhaustion). The full molecular analysis took less than 150 min per sample and revealed no mutation in any of the seven microsatellite sequences in five MSS samples and four to six mutated ones in seven MSI-High samples.ConclusionAt the era when the determination of MSI/MSS status is becoming important for rapid treatment choices, the Idylla MSI Assay consists of a valuable easy-to-perform diagnostic tool that allows, complementary to MLH1, PMS2, MSH2 and MSH6 immunohistochemistry, the diagnosis of MSI/MSS status in a single day.

scholarly journals Occurrence of Cymbasoma longispinosum Bourne, 1890 (Copepoda: Monstrilloida) in the Curuçá River estuary (Amazon Littoral)

2010 ◽  
Vol 82 (3) ◽  
pp. 577-583 ◽  
Author(s):  
Natália R. Leite ◽  
Luci C.C. Pereira ◽  
Fernando Abrunhosa ◽  
Marcus A.B. Pires ◽  
Rauquírio M. da Costa
Keyword(s):  
River Estuary ◽  
Environmental Parameters ◽  
Reproductive Period ◽  
Subsurface Water ◽  
Dry Period ◽  
Rainy Period ◽  
Amazon Estuary ◽  
North Brazil ◽  
Number Of Individuals

The present work was carried out to verify the occurrence and distribution of Cymbasoma longispinosum Bourne, 1890 in a tropical Amazon estuary from North Brazil. Samplings were performed bimonthly from July/2003 to July/2004 at two different transects (Muriá and Curuçá rivers) situated along the Curuçá estuary (Pará, North Brazil). Samples were collected during neap tides via gentle (1 to 1.5 knots) 200 μm-mesh net tows from a small boat. Additional subsurface water samples were collected for the determination of environmental parameters. Males and females of Cymbasoma longispinosum were only observed during September and November/2003. The highest number of organisms was found in September/2003 at the Muriá River transect. The presence of C. longispinosum in samples obtained during September and November/2003 could probably be related to the reproductive period of this species in the studied estuary, which is directly related to the dry period in the region. The highest salinity values and the highest number of individuals observed in September/2003 corroborate with the previous assumption, since no C. longispinosum was found during the months comprising the rainy period (January to June).

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