A comprehensive insight from microalgae production process to characterization of biofuel for the sustainable energy

Background: Lignocellulosic residues generated by various anthropogenic activities can be a potential raw material for many commercial products such as biofuels, organic acids and nutraceuticals including xylitol. Xylitol is a low-calorie nutritive sweetener for diabetic patients. Microbial production of xylitol can be helpful in overcoming the drawbacks of traditional chemical production process and lowring cost of production. Objective: Designing efficient production process needs the characterization of required enzyme/s. Hence current work was focused on in-vitro and in-silico characterization of xylose reductase from Emericella nidulans. Methods: Xylose reductase from one of the hyper-producer isolates, Emericella nidulans Xlt-11 was used for in-vitro characterization. For in-silico characterization, XR sequence (Accession No: Q5BGA7) was used. Results: Xylose reductase from various microorganisms has been studied but the quest for better enzymes, their stability at higher temperature and pH still continues. Xylose reductase from Emericella nidulans Xlt-11 was found NADH dependent and utilizes xylose as its sole substrate for xylitol production. In comparison to whole cells, enzyme exhibited higher enzyme activity at lower cofactor concentration and could tolerate higher substrate concentration. Thermal deactivation profile showed that whole cell catalysts were more stable than enzyme at higher temperature. In-silico analysis of XR sequence from Emericella nidulans (Accession No: Q5BGA7) suggested that the structure was dominated by random coiling. Enzyme sequences have conserved active site with net negative charge and PI value in acidic pH range. Conclusion: Current investigation supported the enzyme’s specific application i.e. bioconversion of xylose to xylitol due to its higher selectivity. In-silico analysis may provide significant structural and physiological information for modifications and improved stability.

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Production Process Effect on Mexican Agave Syrups Quality: A Preliminary Study

Journal of Food Research ◽

10.5539/jfr.v7n3p50 ◽

2018 ◽

Vol 7 (3) ◽

pp. 50

Author(s):

Blanca I. Maldonado-Guevara ◽

Sandra T. Martín del Campo ◽

Anaberta Cardador-Martínez

Keyword(s):

Production Process ◽

Industrial Process ◽

Quality Parameters ◽

Thermal Hydrolysis ◽

Process Variables ◽

Anova Analysis ◽

Hydroxymethyl Furfural ◽

Wide Range ◽

Preliminary Study

In this work, the quality parameters of commercial agave syrups produced in five different Mexican states and with different production process were evaluated. Regulated parameters (pH, Moisture, Ashes, and 5-(hydroxymethyl) furfural), as well as color and aw, were measured on 25 agave syrups, including traditional samples as controls. Traditional and semi-industrial syrups were samples obtained by thermal hydrolysis. Additionally, the semi-industrial process included control of process variables such as pH, °Bx, and temperature. The industrial process is technified. The agave syrups ranged from 70-76 °Bx, pH ranged from 3.2-6.7, and moisture from 20.2-28.6%. The aw values shown a wide variation as well as L* a* and b* color parameters. Some of those parameters shown significant differences in ANOVA analysis; however, most of the samples complied with the norm. General Discriminant Analysis (GDA) made it possible to discriminate between production process by using pH, % Ash, b*, 5-(hydroxymethyl)furfural and a* parameters. Further analysis of a wide range of syrups and the inclusion of non-regulated compounds such as volatile compounds and carbohydrates are needed to get more information for a deeper characterization of agave syrups.

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CARACTERIZAÇÃO HIDRÁULICA DO MICROASPERSOR DAN SPRINKLERS GRUPO MODULAR

Irriga ◽

10.15809/irriga.2001v6n1p13-18 ◽

2001 ◽

Vol 6 (1) ◽

pp. 13-18 ◽

Cited By ~ 1

Author(s):

Marcio Antonio Vilas Boas ◽

Eurides Kuster Macedo Júnior ◽

Silvio Cesar Sampaio ◽

Melânia Inês Valiati

Keyword(s):

Production Process ◽

Power Function ◽

Characteristic Equation ◽

State University ◽

The West ◽

Potential Equation ◽

Group 2 ◽

The Relationship

CARACTERIZAÇÃO HIDRÁULICA DO MICROASPERSOR DAN SPRINKLERS GRUPO MODULAR Márcio Antônio Vilas BoasEurides Kuster Macedo JuniorSilvio César SampaioMelânia Inês ValiatiUNIOESTE - Universidade Estadual do Oeste do ParanáCEP: 85814-110 - Cascavel – PR - Brasil - Cx. Postal 711Fone: (045) 225 -2100 (R-249) - Fax : (045) [email protected] 1 RESUMO Este trabalho teve como objetivo avaliar as características hidráulicas do microaspersor DAN SPRINKLERS do grupo modular de fabricação da DAN SPRINKLERS - ISRAEL, de uso recente no Oeste do Paraná. Os ensaios foram realizados no Laboratório de Hidráulica do Departamento de Engenharia da Universidade Estadual do Oeste do Paraná – UNIOESTE. Na avaliação dos microaspersores estudou-se, a variação decorrente do processo de fabricação e a determinação da equação característica da relação vazão–pressão. Os microaspersores do Grupo modular com diâmetros de bocais 0,94; 1,16;1,41;1,92 e 2,34 mm, foram submetidos às pressões de 100, 150, 200, 250, 300 e 350 kPa. As equações características determinadas indicaram que o microaspersor testado não é auto-compensante , tolerante a sensibilidade de variações de pressões e que a equação potencial se ajusta bem aos dados. Os coeficientes de variação de fabricação obtidos foram menores que 5%, classificando-se, de acordo com a Norma ISO, como de categoria A. UNITERMOS: Microaspersão, coeficiente de variação, modelo potencial. VILAS BÔAS, M. A., MACEDO JUNIOR, E. K. HYDRAULIC CHARACTERIZATION OF MICROSPRINKLER DAN SPRINKLER - MODULATE GROUP 2 ABSTRACT This work had as objective to evaluate the characteristics hydraulic of the microsprinklers of the group to modulate of production of DAN SPRINKLERS - ISRAEL, of recent use in the West of Paraná. The tests was accomplished in the Laboratory of Hydraulics of the Department of Engineering of the State University of the West of Paraná - UNIOESTE. In the evaluation of the microasprinklers it was studied such characteristics as, the variation due to the production process and the determination of the characteristic equation of the relationship vazão-pressure. The microsprinklers of the Group to modulate with diameters of nozzle 0,94; 1,16;1,41;1,92 and 2,34 mm, the pressures were submitted 100, 150, 200, 250, 300 and 350 kPa. The certain characteristic equations showed that the tested microsprinklers is not solemnity-compensante and that the potential equation was fit well to the data. The obtained coefficients of production variation were everybody below 5% being able to not this way to classify them in agreement with for ISO category as A. KEYWORDS: Microsprinkler, coefficient variation, power function.

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