A R2R3-MYB transcription factor, GmMYB12B2, affects the expression levels of flavonoid biosynthesis genes encoding key enzymes in transgenic Arabidopsis plants

Gene ◽  
2013 ◽  
Vol 532 (1) ◽  
pp. 72-79 ◽  
Author(s):  
Xiao-Wei Li ◽  
Jing-Wen Li ◽  
Ying Zhai ◽  
Yan Zhao ◽  
Xu Zhao ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transgenic Arabidopsis ◽  
Flavonoid Biosynthesis ◽  
Myb Transcription Factor ◽  
Key Enzymes ◽  
Expression Levels ◽  
Transgenic Arabidopsis Plants ◽  
Genes Encoding ◽  
R2r3 Myb

Ectopic expression of a R2R3 MYB transcription factor of dove tree (Davidia involucrata) aggravates seed abortion in Arabidopsis thaliana

2020 ◽  
Vol 47 (5) ◽  
pp. 454
Author(s):  
Jian Li ◽  
Tian Chen ◽  
Fengzhen Huang ◽  
Penghui Dai ◽  
Fuxiang Cao ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transcription Factor ◽  
Transgenic Plants ◽  
Ectopic Expression ◽  
Myb Transcription Factor ◽  
Seed Abortion ◽  
Transgenic Seeds ◽  
Genes Encoding ◽  
Colour Changes ◽  
R2r3 Myb

Serious seed abortion of dove tree (Davidia involucrate Baill.) is one of the critical factors leading to the low fecundity of this species. Seed abortion is a complicated process and various factors have been verified to synergistically determine the fate of seeds. To reveal the mechanism of seed abortion in D. involucrata, we performed transcriptome analysis in normal and abortive seeds of D. involucrata. According to the transcriptome data, we noticed that most of the genes encoding a MYB transcription factor were predominantly expressed in abortive seeds. Among these, a gene named DiMYB1 was selected and its function was validated in this study. Overexpression of DiMYB1 resulted in obviously reduced viability of transgenic seeds and seedlings, and caused a significantly higher seed abortion rate. The vegetative growth of transgenic plants was hindered, resulting in an earlier flowering time. In addition, colour changes occurred in transgenic plants. Some transgenic sprouts, stems and pods appeared purple instead of green in colour. Our finding demonstrated that DiMYB1 participates in multiple plant developmental processes, especially in seed development in Arabidopsis thaliana (L.) Heynh., which indicated the similar role of this gene in D. involucrata.

scholarly journals Ectopic Expression of a R2R3-MYB Transcription Factor Gene LjaMYB12 from Lonicera japonica Increases Flavonoid Accumulation in Arabidopsis thaliana

2019 ◽  
Vol 20 (18) ◽  
pp. 4494 ◽  
Author(s):  
Xiwu Qi ◽  
Hailing Fang ◽  
Zequn Chen ◽  
Zhiqi Liu ◽  
Xu Yu ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Ectopic Expression ◽  
Flavonoid Biosynthesis ◽  
Lonicera Japonica ◽  
Myb Transcription Factor ◽  
Flavonoid Content ◽  
Transcription Factor Gene ◽  
Important Indicator ◽  
Factor Gene ◽  
R2r3 Myb

Lonicera japonica Thunb. is a widely used medicinal plant and is rich in a variety of active ingredients. Flavonoids are one of the important components in L. japonica and their content is an important indicator for evaluating the quality of this herb. To study the regulation of flavonoid biosynthesis in L. japonica, an R2R3-MYB transcription factor gene LjaMYB12 was isolated and characterized. Bioinformatics analysis indicated that LjaMYB12 belonged to the subgroup 7, with a typical R2R3 DNA-binding domain and conserved subgroup 7 motifs. The transcriptional level of LjaMYB12 was proportional to the total flavonoid content during the development of L. japonica flowers. Subcellular localization analysis revealed that LjaMYB12 localized to the nucleus. Transactivation activity assay indicated that LjaMYB12 was a transcriptional activator. Then, ectopic expression of LjaMYB12 in Arabidopsis could increase PAL activity and flavonoid content and promote transcription of a range of flavonoid biosynthetic genes. Interestingly, the fold changes of downstream genes in the flavonoid biosynthetic pathway were significantly higher than that of the upstream genes, which suggested that LjaMYB12 may have different regulatory patterns for the upstream and downstream pathways of flavonoid biosynthesis. The results provided here will effectively facilitate the study of subgroup 7 MYBs and transcriptional regulation of flavonoid biosynthesis in L. japonica.

scholarly journals Overexpression of the Wild Soybean R2R3-MYB Transcription Factor GsMYB15 Enhances Resistance to Salt Stress and Helicoverpa Armigera in Transgenic Arabidopsis

2018 ◽  
Vol 19 (12) ◽  
pp. 3958 ◽  
Author(s):  
Xin-Jie Shen ◽  
Yan-Yan Wang ◽  
Yong-Xing Zhang ◽  
Wei Guo ◽  
Yong-Qing Jiao ◽  
...  
Keyword(s):  
Salt Stress ◽  
Transgenic Plants ◽  
Transcriptional Activation ◽  
Transgenic Arabidopsis ◽  
Wild Soybean ◽  
Stress Factors ◽  
Myb Transcription Factor ◽  
Molecular Evidence ◽  
Expression Levels ◽  
R2r3 Myb

Plant R2R3-MYB transcription factors (TFs) have been suggested to play crucial roles in the response to diverse abiotic and biotic stress factors but there is little molecular evidence of this role in soybean plants. In this work, we identified and functionally characterized an R2R3-MYB TF, namely, GsMYB15, from the wild soybean ED059. Protein and promoter sequence analysis indicated that GsMYB15 is a typical R2R3-MYB TF and contains multiple stress-related cis-elements in the promoter region. GsMYB15 is located in the nucleus and exhibits transcriptional activation activity. QPCR assays suggested that the expression of GsMYB15 could be induced by NaCl, insect attacks and defense-related hormones (MeJA and SA). Furthermore, GsMYB15 exhibited highest expression in pods compared to other tissues. Functional analysis of GsMYB15 demonstrated that overexpression of GsMYB15 could increase salt tolerance and enhance the resistance to H. armigera larvae in transgenic Arabidopsis plants. Moreover, overexpression of GsMYB15 also affected the expression levels of salt stress- and defense-related genes in the transgenic plants. Feeding with transgenic Arabidopsis plant leaves could significantly suppress the expression levels of immunity-related genes in H. armigera larvae. Overexpression of GsMYB15 also increased mesophyll cell levels in transgenic plants. Taken together, these results provide evidence that GsMYB15 is a positive regulator of salt stress tolerance and insect resistance in transformed Arabidopsis plants.

R2R3-MYB transcription factor PpMYB17 positively regulates flavonoid biosynthesis in pear fruit

Planta ◽  
2020 ◽  
Vol 252 (4) ◽  
Author(s):  
Apekshika T. Premathilake ◽  
Junbei Ni ◽  
Songling Bai ◽  
Ruiyan Tao ◽  
Mudassar Ahmad ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Flavonoid Biosynthesis ◽  
Myb Transcription Factor ◽  
Pear Fruit ◽  
R2r3 Myb
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