1015 Room Temperature Water Versus Warm Water Immersion for Minimal Sedation Colonoscopy - a Double-Blind, Randomized, Single-Center Trial

2013 ◽  
Vol 77 (5) ◽  
pp. AB177
Author(s):  
Premysl Falt ◽  
Vit Smajstrla ◽  
Martin Kliment ◽  
Josef Tvrdik ◽  
Petr Fojtik ◽  
...  
2018 ◽  
Vol 115 (6) ◽  
pp. 1204-1209 ◽  
Author(s):  
Raffaele Faoro ◽  
Margherita Bassu ◽  
Yara X. Mejia ◽  
Till Stephan ◽  
Nikunj Dudani ◽  
...  

Cryogenic fluorescent light microscopy of flash-frozen cells stands out by artifact-free fixation and very little photobleaching of the fluorophores used. To attain the highest level of resolution, aberration-free immersion objectives with accurately matched immersion media are required, but both do not exist for imaging below the glass-transition temperature of water. Here, we resolve this challenge by combining a cryoimmersion medium, HFE-7200, which matches the refractive index of room-temperature water, with a technological concept in which the body of the objective and the front lens are not in thermal equilibrium. We implemented this concept by replacing the metallic front-lens mount of a standard bioimaging water immersion objective with an insulating ceramic mount heated around its perimeter. In this way, the objective metal housing can be maintained at room temperature, while creating a thermally shielded cold microenvironment around the sample and front lens. To demonstrate the range of potential applications, we show that our method can provide superior contrast in Escherichia coli and yeast cells expressing fluorescent proteins and resolve submicrometer structures in multicolor immunolabeled human bone osteosarcoma epithelial (U2OS) cells at −140°C.


2021 ◽  
Vol 1206 (1) ◽  
pp. 012002
Author(s):  
Satadru Kashyap ◽  
Debanil Das

Abstract Composite materials are known to have many advantages and they can be used in the manufacture of a wide variety of products which make them commercially valuable. However, they may come into contact with various conditions that might lead to weathering. Weathering might lead to discoloration, decrease in durability, decline in mechanical properties and decrease in mass in composites. Hence, it is paramount to study the effects of weathering on composites before they are used in service. For this study, wood polyester composite (WPC), jute polyester composite (JPC) and coir polyester composite (CPC) were fabricated and subjected to four weathering tests - water ageing test at room temperature, water immersion test at 100°C, exposure to external environment and exposure to UV rays. It was seen that CPC samples subjected to water ageing at room temperature and immersion at 100°C showed comparatively higher water absorption rate than the WPC and JPC samples as coir fibres in CPC are hollow at the centremost region. JPC and CPC also turned whitish after 24 hours of water ageing. However, exposing the samples to the environment for a period of 144 hours did not show any significant change in the sample. It was also found that the tensile strength of all the composites decreased after being exposed to UV rays in a QUV spray weathering tester. Additionally, the mechanical properties of the composites were also modelled with analytical techniques (Halphin Tsai method) and FEM analysis and the results were found to be analogous.


1996 ◽  
Vol 32 (8) ◽  
pp. 761 ◽  
Author(s):  
C.R. Kurkjian ◽  
M.J. Matthewson

Pain Practice ◽  
2016 ◽  
Vol 17 (5) ◽  
pp. 669-677 ◽  
Author(s):  
Nagy A. Mekhail ◽  
Emad Estemalik ◽  
Gerges Azer ◽  
Kristina Davis ◽  
Stuart J. Tepper

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