Abstract
Photobiomodulation (PBM) has been used as a bio stimulatory tool for adipose-derived mesenchymal stem cells (ADMSCs). The goal of this in vitro research was to examine the effects of combined and/or single applications of near infra-red (NIR) and green PBM using 5 J/cm2 on ADMSCs. The results indicated that the viability of ADMSCs are not affected by single or combined wavelengths of 525 and 825 nm at 5 J/cm2. However, PBM significantly stimulated cell metabolism seen by an increase in proliferation, it also upregulated intracellular ROS and MMP using 825 nm and 525 nm wavelengths. The combined wavelength irradiation mimicked results found for 825 nm during ATP measurement, cell concentration and migration rate, however significant MMP stimulation and increased ROS production was achieved. In conclusion, results indicate that the combination wavelengths of 525 and 825 nm can be used in the expansion and differentiation of ADMSCs for regenerative purposes.