The association between PON1 and GSTM1 genetic variation with methylation of p16 gene promoter among Javanese farmers exposed to pesticides at Magelang Regency, Central Java, Indonesia

Inactivation of tumor suppressor genes via promoter hypermethylation may play an important role in the progression from Barrett's esophagus (BE) to esophageal adenocarcinoma (EA). We have previously shown that acid-induced p16 gene promoter hypermethylation may depend on activation of NADPH oxidase NOX5-S in BAR-T cells and OE33 EA cells. DNA methyltransferase 1 (DNMT1) is known to participate in maintaining established patterns of DNA methylation in dividing cells and may play an important role in the development of cancer. Therefore, we examined whether DNMT1 is involved in acid-induced p16 gene promoter hypermethylation in BAR-T cells. We found that the acid significantly increased p16 gene promoter methylation, decreased p16 mRNA, and increased cell proliferation, effects that may depend on activation of DNMT1 in BAR-T cells. DNMT1 is overexpressed in EA cells FLO and OE33 and EA tissues. Acid treatment upregulated DNMT1 mRNA expression and increased DNMT1 promoter activity. Acid-induced increases in DNMT1 mRNA expression and promoter activity were significantly decreased by knockdown of NOX5-S and NF-κB1 p50. Conversely, overexpression of NOX5-S, p50, or p65 significantly increased DNMT1 promoter activity. Knockdown of NOX5-S significantly decreased the acid-induced increase in luciferase activity in cells transfected with pNFκB-Luc. An NF-κB binding element GGGGTATCCC was identified in the DNMT1 gene promoter. We conclude that the acid-induced increase in p16 gene promoter methylation, downregulation of p16 mRNA, and increase in cell proliferation may depend on activation of DNMT1 in BAR-T cells. Acid-induced DNMT1 expression may depend on sequential activation of NOX5-S and NF-κB1 p50.

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The relationship between P16 gene promoter methylation and gastric cancer: A meta-analysis based on Chinese patients

Journal of Cancer Research and Therapeutics ◽

10.4103/0973-1482.151535 ◽

2014 ◽

Vol 10 (8) ◽

pp. 292 ◽

Cited By ~ 6

Author(s):

Guoping Sun ◽

Defeng Peng ◽

Heng Zhang

Keyword(s):

Gastric Cancer ◽

Promoter Methylation ◽

Meta Analysis ◽

Gene Promoter ◽

Chinese Patients ◽

P16 Gene ◽

The Relationship

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Medical genetics: advances in brief: Genetic variation in bilirubin UDP-glucuronosyltransferase gene promoter and Gilbert's syndrome

Journal of Medical Genetics ◽

10.1136/jmg.33.8.718-b ◽

1996 ◽

Vol 33 (8) ◽

pp. 718-718

Author(s):

A. Barnicoat

Keyword(s):

Genetic Variation ◽

Gene Promoter ◽

Medical Genetics ◽

Gilbert’S Syndrome ◽

Gilbert's Syndrome ◽

Udp Glucuronosyltransferase

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Genetic diversity of strawberry cultivars in Banyuroto, Magelang, Indonesia based on Cleaved Amplified Polymorphic Sequence

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d200631 ◽

2019 ◽

Vol 20 (6) ◽

Author(s):

MUHAMMAD FAUZI ARIF ◽

GANIES RIZA ARISTYA ◽

RINA SRI KASIAMDARI

Keyword(s):

Genetic Diversity ◽

Genetic Variation ◽

Dna Amplification ◽

Molecular Study ◽

Cleaved Amplified Polymorphic Sequence ◽

Central Java ◽

Ctab Method ◽

Polymorphic Bands ◽

Caps Markers ◽

Strawberry Cultivars

Abstract. Arif MF, Aristya GR, Kasiamdari RS. 2019. Genetic diversity of strawberry cultivars in Banyuroto, Magelang, Indonesia based on Cleaved Amplified Polymorphic Sequence. Biodiversitas 20: 1721-1728. Banyuroto Village, Magelang, Central Java is the center of strawberry (Fragaria spp.) development and cultivation program. The mild climate makes the location very suitable for developing sub-tropic horticulture such as strawberry. Various kinds of cultivars have been developed as part of the agrotourism program. However, genetic variation research of those cultivars is still rarely done. Therefore, the molecular study of strawberry was conducted to determine the genetic variation using CAPS markers. DNA of Five strawberry cultivars from Banyuroto and seven strawberry cultivars from Indonesian Citrus and Subtropical Fruits Research Institute, Malang were isolated using the CTAB method. DNA amplification was performed by PCR using four pairs of primers named APx, OLP, F3H2, and CTI2. The amplification results were cut with three kinds of restriction endonuclease enzymes named MboI, MluI, and TaqI. Restriction product was used to analyze the genetic variation of twelve cultivars and to construct the dendrogram using MVSP software with the UPGMA algorithm. The result showed that the percentage of polymorphic was 45% from 9 polymorphic bands. Dendrogram result showed that there were four clusters. Cluster A consists of Stroberi Hitam, Cluster B consists of Californica Cultivars, cluster C consists of Osso Grande and Osso Purbalingga cultivars, and cluster D consists of Rosalinda, Deeprose, Dorit, Earlibrite, Tristar, Festival, Brastagi, and Aerut. This research concluded that CAPS markers can be used as a method for study genetic diversity of strawberries.

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