Computational study and in vitro alpha-glucosidase inhibitory effects of medicinal plants from a Thai folk remedy

In a continuing effort to discover more anti-inflammatory medicinal plants in China, the anti-inflammatory activities of 101 extracts from different parts of 84 traditional medicinal plants were evaluated by a panel of in vitro and in vivo assays. Nuclear factor-kappa B (NF-κB) inhibitory effects were determined by luciferase assay in stably transfected Hela cells. Cytotoxic activities were assessed using the MTT assay. Inhibitory effects on LPS-induced nitric oxide production and proinflammatory mediators were assessed by Griess reaction and Real-Time PCR analysis, respectively. In vivo anti-inflammatory activities were examined by xylene-induced mice ear edema model. In total, 22 extracts showed promising NF-κB inhibitory effects whereas 9 of them did not affect the cell viability. The 9 hit extracts were active in at least one of the subsequently performed in vitro pharmacological test systems. The extract from Hemerocallis minor (root) was selected to perform the in vivo study because it demonstrated significant suppressive effects in all the in vitro assays. Results showed that the extract of Hemerocallis minor (Root) was able to alleviate ear edema effectively in xylene-induced mice ear edema mode. Collectively, our study provides evidence for the potential anti-inflammatory effects of the medicinal plants traditionally used in China. Further phytochemical and pharmacological studies remain to be clarified.

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UJI IN VITRO DAN IN SILICO SENYAWA 5,7,2’,5’-TETRAHYDROXY FLAVAN-3-OL TERHADAP ENZIM ALPHA GLUCOSIDASE

Jurnal Fitofarmaka Indonesia ◽

10.33096/jffi.v5i2.416 ◽

2018 ◽

Vol 5 (2) ◽

pp. 279-283

Author(s):

Frengki Frengki ◽

Deddi Prima ◽

Fatma Sri Wahyuni ◽

Daan Khambri ◽

Henny Vanda ◽

...

Keyword(s):

Medicinal Plants ◽

Ethyl Acetate ◽

In Silico ◽

Ethyl Acetate Fraction ◽

Stem Bark ◽

Antidiabetic Activity ◽

Inhibition Activity ◽

Antidiabetic Effect ◽

Alpha Glucosidase

Several of Calophyllums genus have been searched and proven as medicinal plants and Calophyllum macrophyllum is one of its genus. We have been isolated a compound from ethyl acetate fraction of the stem-bark. The compound was flavan-3-ol (5,7,2’,5’-tetrahydroxy flavan-3-ol). This research aimed to determine inhibition antidiabetic activity and affinity of its compound on α-glycosidase enzyme. In vitro antidiabetic effect shown by IC50 9.10 µg/ml and docking by Arguslab 4.01 shown by ΔG -10,24 kkal/mol as in silico test. The inhibition activity of flavan-3-ol against the α-glucosidase enzyme that is very strong shows its promising potential as a candidate for antidiabetic drugs.

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Inhibitory effects of different medicinal plants on Candida albicans growth

Medycyna Weterynaryjna ◽

10.21521/mw.5995 ◽

2018 ◽

Vol 74 (1) ◽

pp. 5995-2018 ◽

Cited By ~ 3

Author(s):

ZORAN TAMBUR ◽

DESANKA CENIĆ MILOŠEVIĆ ◽

IVAN MILEUSNIĆ ◽

RADOJE DODER ◽

MARJAN MARJANOVIĆ ◽

...

Keyword(s):

Candida Albicans ◽

Medicinal Plants ◽

Essential Oil ◽

Essential Oils ◽

Dilution Method ◽

Agar Dilution Method ◽

Inhibitory Effects ◽

Strong Activity ◽

Ethanol Extracts

The objective of this study was to evaluate the in vitro antifungal properties of ethanol extracts and essential oils of medicinal plants from Serbia against Candida albicans (C. albicans) ATCC 10231. Ethanol extracts of fifteen plants were investigated, and their effects were compared with those of three different essential oils. The sensitivity of C. albicans to all plants was tested by the agar dilution method. The assay plates were estimated to contain 300, 150, 75, and 37.5 µg/ml of active extracts and 100, 50, 25, and 12.5 µg/ml of active essential oils. Inocula were applied to agar surfaces, giving approximately 106 cfu/ml of C. albicans. No inhibitory effects were observed for ethanol extracts of Hypericum perforatum and Salvia officinalis (MIC > 300 µg/ml). The most effective were the ethanol extract of Aesculus hippocastanum (MIC = 37.5 µg/ml) and the essential oil of Satureja kitaibelii (MIC = 12.5 µg/ml). Other plants showed MIC from 25 to 300 µg/ml. As far as we know, the inhibitory effects of these medicinal plants against the reference strain of C. albicans have not been commonly investigated in our country. Although the essential oil of Satureja kitaibelii shows strong activity against C. albicans, these results need clinical evaluation..

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In Vitro Antidiabetic and Antioxidant Effects of Different Extracts of Catharanthus roseus and Its Indole Alkaloid, Vindoline

Molecules ◽

10.3390/molecules25235546 ◽

2020 ◽

Vol 25 (23) ◽

pp. 5546

Author(s):

Mediline Goboza ◽

Mervin Meyer ◽

Yapo G. Aboua ◽

Oluwafemi O. Oguntibeju

Keyword(s):

Catharanthus Roseus ◽

Plant Extracts ◽

High Glucose ◽

Antioxidant Activities ◽

Scientific Evidence ◽

Indole Alkaloid ◽

Alpha Amylase ◽

Inhibitory Effects ◽

Alpha Glucosidase

The Catharanthus roseus plant has been used traditionally to treat diabetes mellitus. Scientific evidence supporting the antidiabetic effects of this plant’s active ingredient-vindoline has not been fully evaluated. In this study, extracts of C. roseus and vindoline were tested for antioxidant activities, alpha amylase and alpha glucosidase inhibitory activities and insulin secretory effects in pancreatic RIN-5F cell line cultured in the absence of glucose, at low and high glucose concentrations. The methanolic extract of the plant showed the highest antioxidant activities in addition to the high total polyphenolic content (p < 0.05). The HPLC results exhibited increased concentration of vindoline in the dichloromethane and the ethylacetate extracts. Vindoline showed noticeable antioxidant activity when compared to ascorbic acid at p < 0.05 and significantly improved the in vitro insulin secretion. The intracellular reactive oxygen species formation in glucotoxicity-induced cells was significantly reduced following treatment with vindoline, methanolic and the dichloromethane extracts when compared to the high glucose untreated control (p < 0.05). Plant extracts and vindoline showed weaker inhibitory effects on the activities of carbohydrate metabolizing enzymes when compared to acarbose, which inhibited the activities of the enzymes by 80%. The plant extracts also exhibited weak alpha amylase and alpha glucosidase inhibitory effects.

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In vitro Antioxidant, PTP-1B Inhibitory Effects and in vivo Hypoglycemic Potential of Selected Medicinal Plants

International Journal of Pharmacology ◽

10.3923/ijp.2013.50.57 ◽

2012 ◽

Vol 9 (1) ◽

pp. 50-57 ◽

Cited By ~ 8

Author(s):

A. Arya ◽

C.Y. Looi ◽

W.F. Wong ◽

M.I. Noordin ◽

S. Nyamathull ◽

...

Keyword(s):

Medicinal Plants ◽

Inhibitory Effects ◽

Ptp 1B

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Evaluating the Inhibitory Effects of Dichloromethane and Methanol Extracts of Salvia macilenta and Salvia officinalis on the Diabetes Marker Enzyme, Alpha-Glucosidase: An Approach for the Treatment of Diabetes

Jundishapur Journal of Natural Pharmaceutical Products ◽

10.5812/jjnpp.109694 ◽

2021 ◽

Vol In Press (In Press) ◽

Author(s):

Hassan Javid ◽

Soheila Moein ◽

Mahmood Reza Moein

Keyword(s):

Glucose Uptake ◽

Methanol Extract ◽

Inhibitory Effect ◽

Salvia Officinalis ◽

Marker Enzyme ◽

Inhibitory Effects ◽

Methanol Extracts ◽

Treatment Of Diabetes ◽

Alpha Glucosidase

Background: Diabetes mellitus is believed to be the most serious metabolic disease. One of the treatments for diabetes is to delay glucose uptake by inhibiting carbohydrate-hydrolyzing enzymes. Alpha-glucosidase inhibitors delay glucose uptake. Objectives: The present study was conducted aiming to evaluate the efficacy of Salvia extracts in inhibiting diabetes marker enzymes and their effects on the treatment of diabetes. Methods: This experimental study was performed in vitro. The studied plants included Salvia macilenta and Salvia officinalis. The inhibitory effects of their dichloromethane and methanol extracts were also investigated. After calculating the percentage of inhibition and IC50, Km and Vmax using GraphPad Prism 7 were also calculated. The statistical analysis was performed employing GraphPad Instat 3 software. Results: The results herein showed that the greatest inhibitory effect on alpha-glucosidase belonged to the methanol extract of S. macilenta with IC50 = 8.73 ± 0.26 mg/mL compared to that of acarbose with IC50 = 8.82 ± 0.14 mg/mL as a standard. The IC50 of dichloromethane extract of S. officinalis was 8.95 ± 0.23 mg/mL. Conclusions: The extracts had significant inhibitory effects on alpha-glucosidase. However, methanol extract of S. macilenta and dichloromethane extract of S. officinalis demonstrated the greatest inhibitory effects on alpha-glucosidase compared to acarbose as a standard.

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In Vitro Glucose Entrapment and Alpha-Glucosidase Inhibition of Mucilaginous Substances from Selected Thai Medicinal Plants

Scientia Pharmaceutica ◽

10.3797/scipharm.0907-17 ◽

2009 ◽

Vol 77 ◽

Cited By ~ 24

Author(s):

Chanida Palanuvej

Keyword(s):

Medicinal Plants ◽

Thai Medicinal Plants ◽

Alpha Glucosidase

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Inhibitory effects of bioaccessible anthocyanins and procyanidins from apple, red grape, cinnamon on α-amylase, α-glucosidase and lipase

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000652 ◽

2020 ◽

pp. 1-9

Author(s):

Pınar Ercan ◽

Sedef Nehir El

Keyword(s):

Inhibitory Activity ◽

Digestive Enzymes ◽

Positive Control ◽

Anthocyanin Content ◽

Inhibitory Effects ◽

Total Anthocyanin ◽

Total Anthocyanin Content ◽

Before And After ◽

Red Grape

Abstract. The goals of this study were to determine and evaluate the bioaccessibility of total anthocyanin and procyanidin in apple (Amasya, Malus communis), red grape (Papazkarası, Vitis vinifera) and cinnamon (Cassia, Cinnamomum) using an in vitro static digestion system based on human gastrointestinal physiologically relevant conditions. Also, in vitro inhibitory effects of these foods on lipid (lipase) and carbohydrate digestive enzymes (α-amylase and α-glucosidase) were performed with before and after digested samples using acarbose and methylumbelliferyl oleate (4MUO) as the positive control. While the highest total anthocyanin content was found in red grape (164 ± 2.51 mg/100 g), the highest procyanidin content was found in cinnamon (6432 ± 177.31 mg/100 g) (p < 0.05). The anthocyanin bioaccessibilities were found as 10.2 ± 1%, 8.23 ± 0.64%, and 8.73 ± 0.70% in apple, red grape, and cinnamon, respectively. The procyanidin bioaccessibilities of apple, red grape, and cinnamon were found as 17.57 ± 0.71%, 14.08 ± 0.74% and 18.75 ± 1.49%, respectively. The analyzed apple, red grape and cinnamon showed the inhibitory activity against α-glucosidase (IC50 544 ± 21.94, 445 ± 15.67, 1592 ± 17.58 μg/mL, respectively), α-amylase (IC50 38.4 ± 7.26, 56.1 ± 3.60, 3.54 ± 0.86 μg/mL, respectively), and lipase (IC50 52.7 ± 2.05, 581 ± 54.14, 49.6 ± 2.72 μg/mL), respectively. According to our results apple, red grape and cinnamon have potential to inhibit of lipase, α-amylase and α-glucosidase digestive enzymes.

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