scholarly journals Genome Editing as A Versatile Tool to Improve Horticultural Crop Qualities

2020 ◽  
Vol 6 (6) ◽  
pp. 372-384
Author(s):  
Yating Chen ◽  
Wenwen Mao ◽  
Ting Liu ◽  
Qianqian Feng ◽  
Li Li ◽  
...  
Keyword(s):  
Genome Editing ◽  
Horticultural Crop ◽  
Versatile Tool

scholarly journals CRISPR-Cas12a (Cpf1): A Versatile Tool in the Plant Genome Editing Tool Box for Agricultural Advancement

2020 ◽  
Author(s):  
Anindya Bandyopadhyay ◽  
Nagesh Kancharla ◽  
vivek javalkote ◽  
santanu dasgupta ◽  
Thomas Brutnell
Keyword(s):  
Genome Editing ◽  
Genome Engineering ◽  
Temperature Stability ◽  
Double Strand Breaks ◽  
Plant Genome ◽  
Strand Breaks ◽  
Guide Rna ◽  
Versatile Tool ◽  
Type V ◽  
Global Population

Global population is predicted to approach 10 billion by 2050, an increase of over 2 billion from today. To meet the demands of growing, geographically and socio-economically diversified nations, we need to diversity and expand agricultural production. This expansion of agricultural productivity will need to occur under increasing biotic, and environmental constraints driven by climate change. Clustered regularly interspaced short palindromic repeats-site directed nucleases (CRISPR-SDN) and similar genome editing technologies will likely be key enablers to meet future agricultural needs. While the application of CRISPR-Cas9 mediated genome editing has led the way, the use of CRISPR-Cas12a is also increasing significantly for genome engineering of plants. The popularity of the CRISPR-Cas12a, the type V (class-II) system, is gaining momentum because of its versatility and simplified features. These include the use of a small guide RNA devoid of trans-activating crispr RNA (tracrRNA), targeting of T-rich regions of the genome where Cas9 is not suitable for use, RNA processing capability facilitating simpler multiplexing, and its ability to generate double strand breaks (DSB) with staggered ends. Many monocot and dicot species have been successfully edited using this Cas12a system and further research is ongoing to improve its efficiency in plants, including improving the temperature stability of the Cas12a enzyme, identifying new variants of Cas12a or synthetically producing Cas12a with flexible PAM sequences. In this review we provide a comparative survey of CRISPR-Cas12a and Cas9, and provide a perspective on applications of CRISPR-Cas12 in agriculture.

scholarly journals CRISPR-Cas Genome editing system: a versatile tool for developing disease resistant crops

Plant Stress ◽  
2022 ◽  
pp. 100056
Author(s):  
Ashwini Talakayala ◽  
Srinivas Ankanagari ◽  
Mallikarjuna Garladinne
Keyword(s):  
Genome Editing ◽  
System A ◽  
Disease Resistant ◽  
Versatile Tool

scholarly journals Genome editing for horticultural crop improvement

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Jiemeng Xu ◽  
Kai Hua ◽  
Zhaobo Lang
Keyword(s):  
Genome Editing ◽  
Crop Production ◽  
Recent Progress ◽  
Crop Improvement ◽  
Horticultural Crop ◽  
Research Attention ◽  
Horticultural Crops ◽  
Yield And Quality ◽  
Horticultural Research

Abstract Horticultural crops provide humans with many valuable products. The improvement of the yield and quality of horticultural crops has been receiving increasing research attention. Given the development and advantages of genome-editing technologies, research that uses genome editing to improve horticultural crops has substantially increased in recent years. Here, we briefly review the different genome-editing systems used in horticultural research with a focus on clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9)-mediated genome editing. We also summarize recent progress in the application of genome editing for horticultural crop improvement. The combination of rapidly advancing genome-editing technology with breeding will greatly increase horticultural crop production and quality.

scholarly journals Recent advances of Cas12a applications in bacteria

2021 ◽  
Author(s):  
Meliawati Meliawati ◽  
Christoph Schilling ◽  
Jochen Schmid
Keyword(s):  
Genome Editing ◽  
Dna Sequences ◽  
Genome Engineering ◽  
Bacterial Genome ◽  
Adaptive Immune System ◽  
Eukaryotic Cell ◽  
Promising Alternative ◽  
Guide Rna ◽  
Recent Advances ◽  
Versatile Tool

Abstract Clustered regularly interspaced short palindromic repeats (CRISPR)-mediated genome engineering and related technologies have revolutionized biotechnology over the last decade by enhancing the efficiency of sophisticated biological systems. Cas12a (Cpf1) is an RNA-guided endonuclease associated to the CRISPR adaptive immune system found in many prokaryotes. Contrary to its more prominent counterpart Cas9, Cas12a recognizes A/T rich DNA sequences and is able to process its corresponding guide RNA directly, rendering it a versatile tool for multiplex genome editing efforts and other applications in biotechnology. While Cas12a has been extensively used in eukaryotic cell systems, microbial applications are still limited. In this review, we highlight the mechanistic and functional differences between Cas12a and Cas9 and focus on recent advances of applications using Cas12a in bacterial hosts. Furthermore, we discuss advantages as well as current challenges and give a future outlook for this promising alternative CRISPR-Cas system for bacterial genome editing and beyond. Key points • Cas12a is a powerful tool for genome engineering and transcriptional perturbation • Cas12a causes less toxic side effects in bacteria than Cas9 • Self-processing of crRNA arrays facilitates multiplexing approaches

scholarly journals SgRNA Expression of CRIPSR-Cas9 System Based on MiRNA Polycistrons as a Versatile Tool to Manipulate Multiple and Tissue-Specific Genome Editing

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Chen Xie ◽  
Yan-Lian Chen ◽  
Dong-Fang Wang ◽  
Yi-Lin Wang ◽  
Tian-Peng Zhang ◽  
...  
Keyword(s):  
Genome Editing ◽  
Tissue Specific ◽  
Versatile Tool

scholarly journals CRISPR-Cas12a (Cpf1): A Versatile Tool in the Plant Genome Editing Tool Box for Agricultural Advancement

2020 ◽  
Vol 11 ◽  
Author(s):  
Anindya Bandyopadhyay ◽  
Nagesh Kancharla ◽  
Vivek S. Javalkote ◽  
Santanu Dasgupta ◽  
Thomas P. Brutnell
Keyword(s):  
Genome Editing ◽  
Plant Genome ◽  
Versatile Tool

scholarly journals Recent Advances in CRISPR/Cas9-Mediated Genome Editing in Dictyostelium

Cells ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 46 ◽  
Author(s):  
Tetsuya Muramoto ◽  
Hoshie Iriki ◽  
Jun Watanabe ◽  
Takefumi Kawata
Keyword(s):  
Homologous Recombination ◽  
Genome Editing ◽  
Recent Progress ◽  
Target Genes ◽  
High Fidelity ◽  
Target Sequence ◽  
Future Perspectives ◽  
Versatile Tool ◽  
Endogenous Genes ◽  
Genome Modifications

In the last 30 years, knockout of target genes via homologous recombination has been widely performed to clarify the physiological functions of proteins in Dictyostelium. As of late, CRISPR/Cas9-mediated genome editing has become a versatile tool in various organisms, including Dictyostelium, enabling rapid high-fidelity modification of endogenous genes. Here we reviewed recent progress in genome editing in Dictyostelium and summarised useful CRISPR vectors that express sgRNA and Cas9, including several microorganisms. Using these vectors, precise genome modifications can be achieved within 2–3 weeks, beginning with the design of the target sequence. Finally, we discussed future perspectives on the use of CRISPR/Cas9-mediated genome editing in Dictyostelium.

scholarly journals Rapid production of SaCas9 in plant-based cell-free lysate for activity testing

2021 ◽  
Author(s):  
Andreas Schiermeyer ◽  
Pedro Cerda-Bennasser ◽  
Thomas Schmelter ◽  
Xin Huang ◽  
Paul Christou ◽  
...  
Keyword(s):  
Genome Editing ◽  
Nuclease Activity ◽  
In Vitro Activity ◽  
Recombinant Production ◽  
Cas9 Nuclease ◽  
Nicotiana Tabacum L ◽  
Rapid Production ◽  
Versatile Tool ◽  
Established Cell

Cas9 nucleases have become the most versatile tool for genome editing projects in a broad range of organisms. The recombinant production of Cas9 nuclease is desirable for in vitro activity assays or the preparation of ribonucleoproteins (RNPs) for DNA-free genome editing approaches. For the rapid production of Cas9, we explored the use of a recently established cell-free lysate from tobacco (Nicotiana tabacum L.) BY-2 cells. Using this system, the 130-kDa Cas9 nuclease from Staphylococcus aureus (SaCas9) was produced and subsequently purified via affinity chromatography. The purified apoenzyme was supplemented with ten different sgRNAs, and the nuclease activity was confirmed by the linearization of plasmid DNA containing cloned DNA target sequences.

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