scholarly journals Genome editing for horticultural crop improvement

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Jiemeng Xu ◽  
Kai Hua ◽  
Zhaobo Lang

Abstract Horticultural crops provide humans with many valuable products. The improvement of the yield and quality of horticultural crops has been receiving increasing research attention. Given the development and advantages of genome-editing technologies, research that uses genome editing to improve horticultural crops has substantially increased in recent years. Here, we briefly review the different genome-editing systems used in horticultural research with a focus on clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9)-mediated genome editing. We also summarize recent progress in the application of genome editing for horticultural crop improvement. The combination of rapidly advancing genome-editing technology with breeding will greatly increase horticultural crop production and quality.

Horticulturae ◽  
2021 ◽  
Vol 7 (8) ◽  
pp. 240
Author(s):  
Stefano Marino

Environmental conditions and nutritional stress may greatly affect crop performance. Abiotic stresses such as temperature (cold, heat), water (drought, flooding), irradiance, salinity, nutrients, and heavy metals can strongly affect plant growth dynamics and the yield and quality of horticultural products. Such effects have become of greater importance during the course of global climate change. Different strategies and techniques can be used to detect, investigate, and mitigate the effects of environmental and nutritional stress. Horticultural crop management is moving towards digitized, precision management through wireless remote-control solutions, but data analysis, although a traditional approach, remains the basis of stress detection and crop management. This Special Issue summarizes the recent progress in agronomic management strategies to detect and reduce environmental and nutritional stress effects on the yield and quality of horticultural crops.


2020 ◽  
Vol 7 (04) ◽  
Author(s):  
A B Priyanshu ◽  
M K Singh ◽  
Mukesh Kumar ◽  
Vipin Kumar ◽  
Sunil Malik ◽  
...  

An experiment was conducted at Horticultural Research Centre, SVP University of Agriculture and Technology, Meerut (UP) during Rabi season of 2018-19 to assess the impact of different INM doses on yield and quality parameters of garlic. A total of ten treatments consisting of combinations of inorganic fertilizers, organic fertilizers and bio-fertilizers like T1- (Control), T2RDF (100:50:50 kg NPK ha-1), T3-RDF + 20 kg sulphur + FYM 20 ton ha-1, T4- RDF + 20 kg sulphur + VC 4 ton ha-1, T5- 75% RDF + 40 kg sulphur + 5 ton FYM ha-1+ PSB 5 kg ha-1, T6-75% RDF + 40 kg sulphur + 2 ton VC + Azotobacter 5 kg ha-1, T7- 75% RDF + 40 kg sulphur + FYM 3 ton + VC 1 ton+ PSB 5 kg + Azotobacter 5kg ha-1, T8- 50% RDF + 40 kg sulphur + FYM 5 ton + VC2 ton + PSB 5 kg ha-1, T9- 50% RDF + 40 kg sulphur + FYM5 ton + VC 2 ton+ Azotobacter 5 kg ha-1and T10- 50% RDF + 40 kg sulphur + FYM 5 ton + VC2 ton + PSB 5 kg + Azotobacter 5 kg ha-1 were used in Randomized Block Design and replicated thrice. Out of these an application of T7 (75% RDF + 40 kg sulphur + FYM3 ton + VC 1 ton ha-1 + PSB 5 kg + Azotobacter 5 kg ha-1) was found to be significantly superior in term of yield and attributing parameters of garlic.


Pathogens ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 320
Author(s):  
Alexander Nilon ◽  
Karl Robinson ◽  
Hanu R. Pappu ◽  
Neena Mitter

Tomato spotted wilt virus (TSWV) is the type member of the genus Orthotospovirus in the family Tospoviridae and order Bunyavirales. TSWV, transmitted by several species of thrips, causes significant disease losses to agronomic and horticultural crops worldwide, impacting both the yield and quality of the produce. Management strategies include growing virus-resistant cultivars, cultural practices, and managing thrips vectors through pesticide application. However, numerous studies have reported that TSWV isolates can overcome host-plant resistance, while thrips are developing resistance to pesticides that were once effective. RNA interference (RNAi) offers a means of host defence by using double-stranded (ds) RNA to initiate gene silencing against invading viruses. However, adoption of this approach requires production and use of transgenic plants and thus limits the practical application of RNAi against TSWV and other viruses. To fully utilize the potential of RNAi for virus management at the field level, new and novel approaches are needed. In this review, we summarize RNAi and highlight the potential of topical or exogenous application of RNAi triggers for managing TSWV and thrips vectors.


Plants ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 601 ◽  
Author(s):  
Lígia Erpen-Dalla Corte ◽  
Lamiaa M. Mahmoud ◽  
Tatiana S. Moraes ◽  
Zhonglin Mou ◽  
Jude W. Grosser ◽  
...  

Horticultural crops, including fruit, vegetable, and ornamental plants are an important component of the agriculture production systems and play an important role in sustaining human life. With a steady growth in the world’s population and the consequent need for more food, sustainable and increased fruit and vegetable crop production is a major challenge to guarantee future food security. Although conventional breeding techniques have significantly contributed to the development of important varieties, new approaches are required to further improve horticultural crop production. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) has emerged as a valuable genome-editing tool able to change DNA sequences at precisely chosen loci. The CRISPR/Cas9 system was developed based on the bacterial adaptive immune system and comprises of an endonuclease guided by one or more single-guide RNAs to generate double-strand breaks. These breaks can then be repaired by the natural cellular repair mechanisms, during which genetic mutations are introduced. In a short time, the CRISPR/Cas9 system has become a popular genome-editing technique, with numerous examples of gene mutation and transcriptional regulation control in both model and crop plants. In this review, various aspects of the CRISPR/Cas9 system are explored, including a general presentation of the function of the CRISPR/Cas9 system in bacteria and its practical application as a biotechnological tool for editing plant genomes, particularly in horticultural crops.


2008 ◽  
pp. 127-136
Author(s):  
Diána Ungai ◽  
Zoltan Győri

The yield and quality of the sugar beet are mainly determined by level crop production system; therefore the effects of foliar fertilization were studied. Our field trials were carried out in 2005  and 2006 in Hajdúböszörmény, at two experimental sites. In our trials the effects of Biomit Plussz, Fitohorm and Kelcare Cu (having high Cu content) as foliar fertilizers, as well as a fungicide with high sulphur content, Cosavet DF, were studied andcompared. Effects of treatments were studied in four replicates. We took root samples at 4 week intervals, starting in August. The quality of root (sucrose, potassium, sodium and alfa-amino N content) was determined from filtrated beet broth, by an automatic beet laboratory system, called VENEMA. Leaf samples were taken three times in the growing season, element composition was measured by ICP-OES.We found that the crop and the sugar yield were significantly influenced by the foliar treatments both of the two years.


2019 ◽  
Vol 29 (4) ◽  
pp. 461-467
Author(s):  
Karen L. Panter ◽  
Timmothy M. Gergeni ◽  
Casey P. Seals ◽  
Andrea R. Garfinkel

High tunnels are gaining popularity for their use in horticultural crop production. However, little is known about the effect of high tunnel orientation on plant growth and development. In this set of studies, we show tunnel orientation does not necessarily affect the production of cut sunflower (Helianthus annuus) and culinary herbs oregano (Origanum vulgare), marjoram (Origanum majorana), and garlic chive (Allium tuberosum). Two high tunnels, one with the long axis oriented north-south (NS) and the other east-west (EW), were used to test the effects of high tunnel orientation on several crops over a 5-year period: cut sunflower (2012 and 2016); marjoram, oregano, and garlic chive (2013 and 2014); and garlic chive (2015). The tunnels are 12 × 16 ft, smaller than those used in commercial production. The size would be appropriate for hobby and seasonal production of horticultural crops for local markets. Cut sunflower stems were similar lengths both years in both high tunnels. Sunflower times to harvest were different between cultivars but not between high tunnels. Oregano fresh weight yields were highest in the NS tunnel in 2013 but similar between tunnels in 2014. Marjoram fresh weights were highest in 2013 in the EW tunnel but highest in 2014 in the NS tunnel. Garlic chive fresh weights were similar between tunnels all 3 years. We show that differences are more a function of innate cultivar characteristics than which way small high tunnels are oriented.


2021 ◽  
Vol 53 (4) ◽  
pp. 473-501
Author(s):  
Muhammad KHAN ◽  
Niaz AHMED

Optimum supply of macro and micronutrients is of critical importance in improving the yield and quality of horticultural crops. Alike, the quality and yield of mango plants significantly increase by balanced application and uptake of macro and micronutrients. However, soil type and characteristics are important factors that directly influence the bio-availability of these nutrients to the plants. In addition, variability in climate has an impact on mango yield in the current scenario. Many scientists have found that mango cultivation in saline soils is a major obstacle to achieving the desired yield and improving quality. Overdose of fertilization is the major factor for the development of saline soils, furthermore, rise in climate temperatures is also a major factor. Therefore, to overcome this problem, nutrient management and the use of balanced fertilizer are the important factors to be controlled. Thus, this review focuses on the performance and importance of essential macro and micronutrients to improve the yield and quality of mango fruits. To understand the effective use of macro and micronutrients, the positive and negative impacts of the nutrients are explained. It is suggested that analyzing the soil, mango fruits, and mango plant leaves for their nutrient status can be useful to formulate fertilization strategies for higher fruit production and quality. Research and development, along with agricultural extension, should focus more on introducing genetically effective mango varieties to improve nutrient and water utilization efficiency.


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