Human papillomavirus detection and p16INK4a expression in cervical lesions: a comparative study

Abstract Background The causative agent of cervical cancer referred to as Human papillomavirus (HPV) remains a real public health problem. Many countries in West Africa, such as Togo have no data on the high-risk HPV (HR-HPV) infection and genotypes distribution. In order to fill the knowledge gap in the field in Togo, the main objective of this study was to determine the prevalence of pre-cancerous lesions of the cervix and HR-HPV genotypes among Togolese women. Methods Samples were collected from 240 women by introducing a swab in the cervix. Then, the screening of precancerous cervical lesions using the visual inspection with acetic acid and lugol (VIA / VIL) was conducted. The HR-HPV genotypes were characterised by real-time multiplex PCR. Results Out of 240 women recruited, 128 (53.3%) were infected by HR-HPV. The most common genotypes were HPV 56 (22.7%), followed by HPV 51 (20.3%), HPV 31 (19.5%), HPV 52 (18.8%) and HPV 35 (17.2%). The least common genotypes were HPV 33 (2.3%) and HPV 16 (2.3%). Among the women, 1.3% (3/240) were positive to VIA/VIL. Conclusion This study allowed HR-HPV genotypes to be characterised for the first time in Lomé, Togo. This will help in mapping the HR-HPV genotypes in West Africa.

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Human papillomavirus in malignant cervical lesions in Surinam, a high-risk country, compared to the Netherlands, a low-risk country

International Journal of Gynecological Cancer ◽

10.1046/j.1525-1438.1999.99020.x ◽

1999 ◽

Vol 9 (3) ◽

pp. 206-211 ◽

Cited By ~ 29

Author(s):

Krul ◽

Van De Vijver ◽

Schuuring ◽

Van Kanten ◽

Peters ◽

...

Keyword(s):

Human Papillomavirus ◽

High Risk ◽

The Netherlands ◽

Low Risk ◽

Cervical Lesions

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Determination of Human Papillomavirus (HPV) Load and Type in High-Grade Cervical Lesions Surgically Resected from HIV-Infected Women during Follow-up of HPV Infection

Clinical Infectious Diseases ◽

10.1086/427032 ◽

2005 ◽

Vol 40 (3) ◽

pp. 451-457 ◽

Cited By ~ 23

Author(s):

F. B. Lillo ◽

S. Lodini ◽

D. Ferrari ◽

C. Stayton ◽

G. Taccagni ◽

...

Keyword(s):

Human Papillomavirus ◽

Hpv Infection ◽

High Grade ◽

Cervical Lesions

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Multiple ASF/SF2 Sites in the Human Papillomavirus Type 16 (HPV-16) E4-Coding Region Promote Splicing to the Most Commonly Used 3′-Splice Site on the HPV-16 Genome

Journal of Virology ◽

10.1128/jvi.00462-10 ◽

2010 ◽

Vol 84 (16) ◽

pp. 8219-8230 ◽

Cited By ~ 33

Author(s):

Monika Somberg ◽

Stefan Schwartz

Keyword(s):

Cervical Cancer ◽

Human Papillomavirus ◽

Splice Site ◽

Binding Sites ◽

Mrna Levels ◽

Coding Region ◽

Cervical Lesions ◽

Hpv 16 ◽

Human Papillomavirus Type 16 ◽

E6 And E7

ABSTRACT Our results presented here demonstrate that the most abundant human papillomavirus type 16 (HPV-16) mRNAs expressing the viral oncogenes E6 and E7 are regulated by cellular ASF/SF2, itself defined as a proto-oncogene and overexpressed in cervical cancer cells. We show that the most frequently used 3′-splice site on the HPV-16 genome, site SA3358, which is used to produce primarily E4, E6, and E7 mRNAs, is regulated by ASF/SF2. Splice site SA3358 is immediately followed by 15 potential binding sites for the splicing factor ASF/SF2. Recombinant ASF/SF2 binds to the cluster of ASF/SF2 sites. Mutational inactivation of all 15 sites abolished splicing to SA3358 and redirected splicing to the downstream-located, late 3′-splice site SA5639. Overexpression of a mutant ASF/SF2 protein that lacks the RS domain, also totally inhibited the usage of SA3358 and redirected splicing to the late 3′-splice site SA5639. The 15 ASF/SF2 binding sites could be replaced by an ASF/SF2-dependent, HIV-1-derived splicing enhancer named GAR. This enhancer was also inhibited by the mutant ASF/SF2 protein that lacks the RS domain. Finally, silencer RNA (siRNA)-mediated knockdown of ASF/SF2 caused a reduction in spliced HPV-16 mRNA levels. Taken together, our results demonstrate that the major HPV-16 3′-splice site SA3358 is dependent on ASF/SF2. SA3358 is used by the most abundantly expressed HPV-16 mRNAs, including those encoding E6 and E7. High levels of ASF/SF2 may therefore be a requirement for progression to cervical cancer. This is supported by our earlier findings that ASF/SF2 is overexpressed in high-grade cervical lesions and cervical cancer.

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Prevalence of human papillomavirus infection and correlation with cervical lesions in Japanese women

Journal of Obstetrics and Gynaecology Research ◽

10.1111/j.1447-0756.2004.00213.x ◽

2004 ◽

Vol 30 (5) ◽

pp. 380-385 ◽

Cited By ~ 7

Author(s):

Kazuhisa Ishi ◽

Fujihiko Suzuki ◽

Shigetaka Yamasaki ◽

Hiroko Suto ◽

Katsunari Kina ◽

...

Keyword(s):

Human Papillomavirus ◽

Japanese Women ◽

Cervical Lesions ◽

Human Papillomavirus Infection ◽

Papillomavirus Infection

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Detection of aflatoxin B1 adducts in Mexican women with cervical lesions

World Mycotoxin Journal ◽

10.3920/wmj2020.2602 ◽

2021 ◽

pp. 1-12

Author(s):

L. Díaz de León-Martínez ◽

C.M. López-Mendoza ◽

Y. Terán-Figueroa ◽

R. Flores-Ramírez ◽

F. Díaz-Barriga ◽

...

Keyword(s):

Human Papillomavirus ◽

Dna Adducts ◽

Hpv Infection ◽

Mexican Women ◽

Cervical Lesions ◽

Increased Risk ◽

Secondary Objective ◽

E6 And E7 ◽

Aflatoxin B ◽

Potent Carcinogen

Cervical cancer (CC) is one of the most serious threats to the lives of women; co-factors in addition to oncogenic human papillomavirus (HPV) infection may be important in causing CC. Women in Mexico are exposed to dietary aflatoxin B1, a potent carcinogen, which may act as a co-factor, in inducing progression to CC. Scarce studies are addressing environmental risks associated with the development of CC, thus the study aimed to establish a relationship between the presence of AFB1 and the detection of human papillomavirus in the genome of Mexican women. Forty samples from cervical tissue of women infected with HPV were obtained; positive results regarding the HPV type (16 and/or 18) were found in 92.5% women and the presence of AFB1-DNA adducts were detected in 77.5% of the same positive HPV samples. Detection of AFB1-DNA adducts and genomic concentrations were correlated with the detection of two oncogenic types of HPV 16 and 18. AFB1-DNA positivity and higher genomic concentrations of AFB1-DNA adducts were correlated with an increased risk of oncogenic detection of HPV in cervical samples from women in Mexico. As a secondary objective, a hypothetical interaction of the adducts with the NRF2 pathway has been proposed, therefore activation of p62 and in turn E6 and E7 (HPV proteins) would inhibit the formation of autophagosomes, which would result in a presence or recurrence of CC.

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