Development of a microtitre plate method for determination of phenol utilization, biofilm formation and respiratory activity by environmental bacterial isolates

2005 ◽  
Vol 56 (4) ◽  
pp. 231-235 ◽  
Author(s):  
Emtiazi Giti ◽  
Hassanshahyan Mehdi ◽  
Golbang Nasser
Keyword(s):  
Biofilm Formation ◽  
Respiratory Activity ◽  
Bacterial Isolates ◽  
Microtitre Plate ◽  
Plate Method

scholarly journals Biofilm formation by bacteria isolated from intensive care units of a tertiary care hospital, with special relevance to its risk factors

2021 ◽  
Vol 9 (10) ◽  
pp. 2959
Author(s):  
Mayuri Gogoi ◽  
Ajanta Sharma
Keyword(s):  
Risk Factors ◽  
Tissue Culture ◽  
Intensive Care ◽  
Biofilm Formation ◽  
P Value ◽  
Bacterial Isolates ◽  
Plate Method ◽  
Culture Plate ◽  
Tissue Culture Plate ◽  
Device Associated Infection

Background: The purpose of this study was to detect biofilm formation by bacterial isolates from patients with device associated infection admitted in intensive care units (ICUs), to compare the three methods used for detection of bioiflm, to compare the antimicrobial susceptibility pattern of the biofilm producers with the non-producers and to study the risk factors associated with biofilm formation.Methods: A total of 115 bacterial isolates from patients with device associated infection admitted in different ICU for a period of one year was included in the study. These clinical isolates were detected for biofilm formation by tissue culture plate method, tube method and Congo red agar method. Kirby-Bauer disc diffusion method of antibiotic susceptibility was performed on all isolates.Results: Out of the 115 bacterial isolates, 71 were biofilm producers. Tissue culture plate method detected the maximum number of biofilm producers (61.7%). The maximum number of biofilm producers were isolated from tracheal aspirate and endotracheal tubes (52.1%) followed by blood (17%) and urine (12.6%) respectively. The predominant biofilm producing isolates were Klebsiella pneumoniae (39.4%), Staphylococcus aureus (19.7%) and Pseudomonas aeruginosa (16.9%). Multi drug resistance among the biofilm producers was significantly higher than the non-biofilm producers (p value=0.0125). The risk of biofilm formation was seen to increase with the increase in duration of hospital stay (p value=0.0092, statistically very significant).Conclusions: From this study it was found that a high degree of biofilm producers were isolated from patients on indwelling devices. Tissue culture plate was found to be the most accurate method. The degree of multidrug resistance among the bioiflm producers was significantly higher than the non-producers.

scholarly journals Inhibitory effects of antifungal drugs on biofilm producing Aspergillus spp. recovered from drinking water system

2020 ◽  
Author(s):  
Noorulain Nazir ◽  
Abubakar Siddique ◽  
Nisar A Khan
Keyword(s):  
Drinking Water ◽  
Amphotericin B ◽  
Crystal Violet ◽  
Biofilm Formation ◽  
Respiratory Diseases ◽  
Antifungal Susceptibility ◽  
Antifungal Drugs ◽  
Microtitre Plate ◽  
Plate Method ◽  
Crystal Violet Assay

Abstract Aims: Biofilms formed in drinking water distribution systems serve as a continuous source of fungal infections. Biofilms are thick aggregates of adherent microorganisms including pathogenic species of fungi. Respiratory diseases and skin allergy reactions are caused by drinking water containing biofilm forming fungus and bacteria. One of the main causes of nosocomial infections and respiratory diseases in hospitals is due to the fungal biofilm formation in machines, catheters and other surgical instruments. There are some antifungal drugs which are used to control biofilm formation to minimize the infection rate. Methodology and results: The present study was conducted to isolate and identify Aspergillus species which are the main fungal spp. responsible for the biofilm formation in drinking water and to check their antifungal susceptibility against antifungal drugs. The isolated fungal samples from drinking water were cultivated on Potato dextrose agar for the isolation of Aspergillus species. Isolated Aspergillus species were identified on the basis of cultural, morphological and microscopic examination. Then in-vitro ability of biofilm produced by isolated Aspergillus species was estimated using microtitre plate method and quantification by crystal violet assay. Antifungal susceptibility testing against isolated fungal spp. was done by antifungal drug Amphotericin B.Results: From results, it is concluded that drinking water of labs, hospitals and common water chillers were more prevelant by Aspergillus species whereas water from reverse osmosis plants showed negative results. From microtitre plate method and crystal violet assay, it was concluded that Aspergillus spp. are Susceptible against Amphotericin B drug as compared to miconazole.

scholarly journals Inhibitory Effects of Antifungal Drugs on Biofilm Producing Aspergillus spp. Recovered from Drinking Water System

2020 ◽  
Vol 7 (2) ◽  
Author(s):  
Nazir NUA ◽  
◽  
Siddique A ◽  
Khan MN ◽  
Ishaque M ◽  
...  
Keyword(s):  
Drinking Water ◽  
Amphotericin B ◽  
Biofilm Formation ◽  
Respiratory Diseases ◽  
Antifungal Susceptibility ◽  
Antifungal Drugs ◽  
Aspergillus Species ◽  
Microtitre Plate ◽  
Plate Method ◽  
Crystal Violet Assay

Aims: Biofilms formed in drinking water distribution systems serve as a continuous source of fungal infections. Biofilms are thick aggregates of adherent microorganisms including pathogenic species of fungi. Respiratory diseases and skin allergy reactions are caused by drinking water containing biofilm forming fungus and bacteria. One of the main causes of nosocomial infections and respiratory diseases in hospitals is due to the fungal biofilm formation in machines, catheters and other surgical instruments. There are some antifungal drugs which are used to control biofilm formation to minimize the infection rate. Methodology and results: The present study was conducted to isolate and identify Aspergillus species which are the main fungal spp. responsible for the biofilm formation in drinking water and to check their antifungal susceptibility against antifungal drugs. The isolated fungal samples from drinking water were cultivated on Potato dextrose agar for the isolation of Aspergillus species. Isolated Aspergillus species were identified on the basis of cultural, morphological and microscopic examination. Then in-vitro ability of biofilm produced by isolated Aspergillus species was estimated using microtitre plate method and quantification by crystal violet assay. Antifungal susceptibility testing against isolated fungal spp. was done by antifungal drug Amphotericin B. Results: From results, it is concluded that drinking water of labs, hospitals and common water chillers were more prevelant by Aspergillus species whereas water from reverse osmosis plants showed negative results. From microtitre plate method and crystal violet assay, it was concluded that Aspergillus spp. are Susceptible against Amphotericin B drug as compared to miconazole. Keywords: Aspergillus spp; Biofilm; Drinking water; Disk diffusion method; Amphotericin B

Biofilm formation and Antibiotic susceptibility pattern among Staphylococcus aureus in a tertiary care hospital in Kanchipuram: An Evaluation of screening methods for biofilm formation

2016 ◽  
Vol 5 (04) ◽  
pp. 4991
Author(s):  
Abirami Lakshmy Jayachandran* ◽  
Sarasa S. ◽  
Sheila Doris T. ◽  
Balan K. ◽  
Sangeetha Vilwanathan ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Susceptibility ◽  
Biofilm Formation ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Microtitre Plate ◽  
Disc Diffusion Method ◽  
Plate Method ◽  
Antibiotic Susceptibility Pattern ◽  
Biofilm Production

The ability of Staphylococcus aureus to form biofilms is of significant clinical interest, as biofilm development impacts the efficacy of antimicrobial therapy and the subsequent outcome of an infection. The present study is undertaken to detect the biofilm production and to determine the antibiotic susceptibility pattern among the Staphylococcus aureus isolates. A total of 100 Staphylococcus aureus isolated for the first time from pus, blood, catheter, IV cannulas were included in the study. Biofilm detection was done by tube method and Microtitre plate method. Antibiotic susceptibility was done by Kirby bauer disc diffusion method. Methicillin resistance was detected by Cefoxitin disc diffusion method. By tube method and Microtitre plate method 26% and 46% of the isolates were identified as biofilm producers. By Microtitre plate method, BHI broth (Brain heart infusion broth) and BHI broth with sucrose was used and the difference in the biofilm forming ability was compared. When BHI broth with sucrose was used 69% showed biofilm formation whereas when tested with BHI broth, only 46% were identified as biofilm producers. Good sensitivity was observed for Amikacin (88%) and cefotaxime (82%). MRSA (Methicillin resistant Staphylococcus aureus) was detected among 19% of the isolates. Among the biofilm producers if there are drug resistant bacteria like MRSA the problem becomes challenging and requires combination of several antibiotics. Hence Screening for biofilm production by bacterial isolates should be performed. Infection control program should address the effective execution of disinfection procedures.

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