The protective effect of monosodium glutamate on survival of Lactobacillus rhamnosus GG and Lactobacillus rhamnosus E-97800 (E800) strains during spray-drying and storage in trehalose-containing powders

Patulin, a mycotoxin, which is a major contaminant in apple juices, has contributed immensely to the occurrence of liver diseases. Consumption of apple juice could over long period of time become harmful to the health of individuals with pre-existing liver disease. Probiotics are known for their role in patulin removal from aqueous media. In this study, we investigated the effects of a probiotic microorganism on patulin toxicity in hepatocellular carcinoma (HepG2) cells and established the protective effect of Lactobacillus rhamnosus (LGG) as mediated by induction of BH3-interacting domain antagonist (BID) in response to patulin toxicity. After 24 hours of patulin exposure followed by 24 hours of treatment with Lactobacillus rhamnosus, cells proliferation decreased with increasing patulin exposure in samples without LGG pre-treatment, whereas with increasing concentration of patulin, cells were relatively rescued in LGG treated samples. It was further observed that pre-treatment of LGG with polysaccharide gums led to a decline in cell proliferation with increasing patulin exposure. Compare to the control, the expression of p53 upregulated moderator of apoptosis (PUMA) increased slightly by 7 % at 10µM patulin exposure in treatment and decreased by 30% in untreated cell. However, the expression of BID decreased by 26% in treatment compared to the control. We further established that the protective effect of Lactobacillus rhamnosus was mediated by the inhibition of BID. Our findings suggest that Lactobacillus rhamnosus GG could potentially function as a therapeutic agent to reverse the damaging effect of patulin on the liver of individuals with pre-existing liver disease.

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Development of milk protein edible films incorporated with Lactobacillus rhamnosus GG

BioResources ◽

10.15376/biores.15.3.6960-6973 ◽

2020 ◽

Vol 15 (3) ◽

pp. 6960-6973

Author(s):

Yun Ying Lee ◽

Elaine ◽

Yus Aniza Yusof ◽

Liew Phing Pui

Keyword(s):

Storage Temperature ◽

Lactobacillus Rhamnosus ◽

Sodium Caseinate ◽

Edible Films ◽

Protein Isolate ◽

Drying Process ◽

Lactobacillus Rhamnosus Gg ◽

Film Forming ◽

Puncture Force ◽

And Storage

Bioactive edible films have the potential to be probiotic carriers. This innovative approach can replace plastic packaging and can benefit human health. This study demonstrated the incorporation of Lactobacillus rhamnosus GG (LGG) into whey protein isolate (WPI) and sodium caseinate (NaCas) edible films. Probiotic cells were directly incorporated into the film forming solutions, and the films were produced by the casting method. The physical, mechanical, and probiotic viability properties of the edible films were determined in the presence and absence of LGG. Furthermore, the viability of LGG was evaluated during the drying process and storage of 14 days at 4 °C and 25 °C, respectively. The results showed the incorporation of LGG increased the moisture content, puncture force, and lightness of both films. However, viability of LGG was lower in the WPI film regardless of storage temperature. At the end of storage days, both WPI and NaCas edible films maintained the LGG viability above the recommended levels when stored at 4 °C, which was 106 CFU/g. The findings of this study suggested that edible films made of WPI and NaCas showed feasibility to immobilize LGG with chilled storage at 4 ℃.

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Protective Effect of Lactobacillus rhamnosus GG and its Supernatant against Myocardial Dysfunction in Obese Mice Exposed to Intermittent Hypoxia is Associated with the Activation of Nrf2 Pathway

International Journal of Biological Sciences ◽

10.7150/ijbs.36465 ◽

2019 ◽

Vol 15 (11) ◽

pp. 2471-2483 ◽

Cited By ~ 4

Author(s):

Hui Xu ◽

Jiqun Wang ◽

Jun Cai ◽

Wenke Feng ◽

Yonggang Wang ◽

...

Keyword(s):

Protective Effect ◽

Intermittent Hypoxia ◽

Myocardial Dysfunction ◽

Lactobacillus Rhamnosus ◽

Obese Mice ◽

Lactobacillus Rhamnosus Gg ◽

Nrf2 Pathway

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Effects of initial cell concentration, growth phase, and process parameters on the viability of Lactobacillus rhamnosus GG after spray drying

Drying Technology ◽

10.1080/07373937.2019.1648290 ◽

2019 ◽

Vol 38 (11) ◽

pp. 1474-1492 ◽

Cited By ~ 3

Author(s):

Géraldine Broeckx ◽

Shari Kiekens ◽

Katarina Jokicevic ◽

Eline Byl ◽

Tim Henkens ◽

...

Keyword(s):

Spray Drying ◽

Process Parameters ◽

Growth Phase ◽

Cell Concentration ◽

Lactobacillus Rhamnosus ◽

Lactobacillus Rhamnosus Gg ◽

Initial Cell Concentration ◽

Initial Cell

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Microencapsulation of Cajuput Oil by Spray Drying: Influence of Wall Systems on the Oil Retention and Storage

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.117-119.1518 ◽

2011 ◽

Vol 117-119 ◽

pp. 1518-1521

Author(s):

Kangsadarn Wicheansin ◽

Ratana Indranupakorn

Keyword(s):

Protective Effect ◽

Spray Drying ◽

Oil Content ◽

Gum Arabic ◽

Modified Starch ◽

Oil Retention ◽

And Storage

The microencapsulation of cajuput oil by spray drying was investigated with respect to the effects of two kinds of matrices (gum arabic and modified starch) as well as to the effects of initial concentrations of cajuput oil on its retention and stability. The results indicated that the oil retention depended on the type of encapsulating agent. Hicap100 showed higher oil retention of encapsulated cajuput oil. Further, the surface oil content of the Hicap100 powder was very low. The protective effect of microencapsulation of Hicap100 was also studied. The functional compound, 1,8-cineole, was released more slowly from the Hicap100 compared to gum arabic, especially at the level of 30% oil loaded.

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