Chitosan-graft-poly(N-hydroxy ethyl acrylamide) copolymers: Synthesis, characterization and preliminary blood compatibility in vitro

2019 ◽  
Vol 123 ◽  
pp. 1257-1266 ◽  
Author(s):  
Ehsan Bahramzadeh ◽  
Elvan Yilmaz ◽  
Terin Adali
Keyword(s):  
Blood Compatibility ◽  
Acrylamide Copolymers

scholarly journals Calcium phosphate phase transition in the presence of Aminosilane

2014 ◽  
Vol 70 (a1) ◽  
pp. C67-C67
Author(s):  
Babak Mostaghaci ◽  
Brigitta Loretz ◽  
Robert Haberkorn ◽  
Guido Kickelbick ◽  
Claus-Michael Lehr
Keyword(s):  
Phase Transition ◽  
Calcium Phosphate ◽  
Blood Compatibility ◽  
Transfection Efficiency ◽  
Hek293 Cells ◽  
Step Method ◽  
Calcium Phosphate Nanoparticles ◽  
Amine Groups ◽  
The Impact

Calcium phosphate has been the point of interest for in vitro gene delivery for many years because of its biocompatibility and straight forward application. However, there are some limitations regarding in vivo administration of these particles mostly because of vast agglomeration of the particles and lack of strong bond between the particles and pDNA. We introduced a simple single step method to functionalize calcium phosphate nanoparticles with Aminosilanes having a different number of amine groups. The nanoparticles were characterized chemically and structurally and their toxicity and interaction with pDNA were studied as well. Results revealed that different crystalline phase of calcium phosphate nanoparticles (Brushite and Hydroxyapatite) with a size below 150 nm were prepared, depending on conditions of synthesis and phase, each with a narrow size distribution. The aminosilane agents caused oriented nucleation and growth of crystallites and can decrease the pH for producing hydroxyapatite phase. The phenomenon could be revealed with the presence of anisotropy in the structure of synthesized hydroxyapatite. The number of amine groups in the Aminosilane agent could change the phase transition pH. Brushite particles revealed to have stronger interaction with pDNA mostly because of their higher positive surface charge. Both particles showed blood compatibility and negligible toxicity. Transfection experiment revealed the capability of both brushite and hydroxyapatite particles to transfect A549 and HEK293 cells. The new modified nanoparticles can be stored in a dried state and re-dispersed easily at the time of administration. Moreover, the transfection efficiency is higher in comparison with conventional calcium phosphate. This study showed the impact of presence and type of the modifying agent on the crystal structure and the amount of surface functionalization of nanoparticles, which in consequence influenced their interaction with cells.

scholarly journals In Vitro Blood Compatibility of Novel Hydrophilic Chitosan Films for Vessel Regeneration and Repair

10.5772/52706 ◽  
2013 ◽  
Author(s):  
Antonello A. ◽  
Luigi Ippolito ◽  
Federica Riccardi ◽  
Silvia Pipitone ◽  
Marina Morganti ◽  
...  
Keyword(s):  
Blood Compatibility ◽  
Chitosan Films ◽  
Vessel Regeneration

Surface Characterization and In Vitro Blood Compatibility of Poly(Ethylene Terephthalate) Immobilized with Hirudin

2005 ◽  
Vol 288-289 ◽  
pp. 421-424
Author(s):  
F. Li ◽  
Jin Wang ◽  
H. Sun ◽  
Nan Huang
Keyword(s):  
Free Energy ◽  
Photoelectron Spectroscopy ◽  
Surface Characterization ◽  
Ethylene Terephthalate ◽  
Blood Compatibility ◽  
Argon Plasma ◽  
Angle Measurement ◽  
Poly Ethylene Terephthalate ◽  
Poly Ethylene

Poly(ethylene terephthalate) films were exposed under argon plasma glow discharge and induced polymerization of acrylic acid (AA) in order to introduce carboxylic acid group onto PET (PET-AA) assisting by ultroviolet radiation. Hirudin-immobilized PETs were prepared by the grafting of PET-AA, followed by chemical reaction with hirudin. The surface structure of the treated PET is determined by X-ray photoelectron spectroscopy (XPS). The wettability and surface free energy, interface free energy of the films is investigated by contact angle measurement. Platelet adhesion evaluatiion is conducted to examine the blood compatibility in vitro. Scanning electron microscopy (SEM) and optical microscopy reveal that the amounts of adhered, aggregated and morphologically changed platelets are reduced on hirudin-immobilized PET films.

In vitro assessment of blood compatibility: Residual and dynamic markers of cellular activation

2011 ◽  
Vol 27 (8) ◽  
pp. 925-936 ◽  
Author(s):  
Greg Johnson ◽  
Benjamin Curry ◽  
Linda Cahalan ◽  
Roni Prater ◽  
Michael Beeler ◽  
...  
Keyword(s):  
Blood Compatibility ◽  
Cellular Activation ◽  
In Vitro Assessment

Study on Chitosan-Based Nanocomposite Hydrogel in Soft Tissue Defect of Hand

2020 ◽  
Vol 12 (9) ◽  
pp. 1120-1126
Author(s):  
Jian Zhang ◽  
Zhaoyu Gao ◽  
Yao Zhang ◽  
Jinghai Tian
Keyword(s):  
Soft Tissue ◽  
Blood Compatibility ◽  
Rabbit Model ◽  
Soft Tissue Defect ◽  
Common Disease ◽  
Nanocomposite Hydrogel ◽  
Soft Tissue Defects ◽  
Clinical Role ◽  
Tissue Defects

Hand soft tissue defects are a common disease in surgical emergency, which seriously affects the limb function of patients and significantly reduces their quality of life. To effectively repair hand soft tissue defects, a difficult problem posed to surgeons, we report here the use of a chitosan-based nanocomposite hydrogel (CS-GEL-PDRN). In vitro experiments showed that the nanocomposite hydrogel could promote the proliferation of human embryonic fibroblasts M-22 and human vascular endothelial cells EC-304. In addition, the hydrogel had excellent blood compatibility. In the rabbit model of soft tissue defects, CS-GEL-PDRN effectively coordinated cytokine responses, accelerated wound healing and promoted tissue repair. Based on this, CS-GEL-PDRN is expected to play a clinical role in the repair of hand and other soft tissue defects in the future.

scholarly journals Novel Biocompatible Zr-Based Alloy with Low Young’s Modulus and Magnetic Susceptibility for Biomedical Implants

Materials ◽  
2020 ◽  
Vol 13 (22) ◽  
pp. 5130
Author(s):  
Renhao Xue ◽  
Dong Wang ◽  
Dawei Yang ◽  
Ligang Zhang ◽  
Xiaoning Xu ◽  
...  
Keyword(s):  
Magnetic Susceptibility ◽  
Young’S Modulus ◽  
Blood Compatibility ◽  
Young's Modulus ◽  
Emission Spectrometry ◽  
Biomedical Implants ◽  
Ion Release ◽  
Mg63 Cells ◽  
Ti Addition

The microstructure, mechanical properties, magnetic susceptibility, electrochemical corrosion performance, in vitro cell compatibility and blood consistency of Zr-16Nb-xTi (x = 0, 4, 8, 12 and 16 wt.%) materials were investigated as potential materials for biomedical implants. X-ray diffraction (XRD) and Transmission electron microscopy (TEM) analyses revealed the secondary phase martensite α’ formed during the quenching process. The phase composition contained metastable β and martensite α’, resulting from Ti addition. These phase constitutions were the main causes of a low Young’s modulus and magnetic susceptibility. The in vitro cytocompatibility analysis illustrated that the MG63 cells maintained high activity (from 91% to 97%) after culturing in Zr-16Nb-xTi extraction media for 12 days due to the high internal biocompatibility of Zr, Nb and Ti elements, as well as the optimal corrosion resistance of Zr-16Nb-xTi. On the basis of Inductively coupled plasma optical emission spectrometry (ICP-OES) ion release studies, the concentration of Zr, Nb and Ti was noted to reach the equipment detective limit of 0.001 mg/L, which was much lower than pure Ti. With respect to the corrosion behavior in Hank’s solution, Zr-16Nb-16Ti displayed superior properties, possessing the lowest corrosion current density and widest passivation region, attributed to the addition of Ti. The blood compatibility test illustrated that the Zr-16Nb-xTi materials were nonhemolytic, and the platelets maintained a spherical shape, with no aggregation or activation on Zr-16Nb-xTi. Overall, Ti addition has obvious effects on the developed Zr-16Nb-xTi alloys, and Zr-16Nb-4Ti exhibited low magnetic susceptibility, low modulus, good biocompatibility and proper corrosion properties, demonstrating the potential of use as implant biomaterials.

Sign in / Sign up

Export Citation Format

Share Document