Effects of an Escherichia coli exopolysaccharide on human and mouse gut microbiota in vitro

2020 ◽  
Vol 150 ◽  
pp. 991-999 ◽  
Author(s):  
Baiyuan Li ◽  
Huahai Chen ◽  
Linyan Cao ◽  
Yunfei Hu ◽  
Dan Chen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gut Microbiota ◽  
Human And Mouse ◽  
Mouse Gut Microbiota

scholarly journals Salicylate blockade of granulocyte adherence and the inflammatory response to experimental peritonitis

Blood ◽  
1979 ◽  
Vol 53 (5) ◽  
pp. 1018-1022
Author(s):  
PJ Spagnuolo ◽  
JJ Ellner
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Streptococcus Pneumoniae ◽  
Inflammatory Response ◽  
Sodium Salicylate ◽  
Nylon Fiber ◽  
Intraperitoneal Infection ◽  
And Staphylococcus Aureus ◽  
Human And Mouse

Aspirin profoundly inhibited the in vitro augmentation of human and mouse granulocyte adherence to nylon fiber induced by the bacterial products Escherichia coli endotoxin and Staphylococcus aureus culture filtrate. Granulocytes obtained from normal volunteers during the 48 hr following ingestion of aspirin did not respond normally to endotoxin stimulation. Furthermore, pretreatment of mice with sodium salicylate prior to intraperitoneal infection with Streptococcus pneumoniae impaired granulocyte exudation and resulted in uncontrolled bacteremia and greater lethality of infection.

scholarly journals Characterizing scaling laws in gut microbial dynamics from time series data: caution is warranted

2021 ◽  
Author(s):  
Xu-Wen Wang ◽  
Yang-Yu Liu
Keyword(s):  
Time Series ◽  
Gut Microbiota ◽  
Scaling Laws ◽  
Time Series Data ◽  
Series Data ◽  
Temporal Variance ◽  
Short And Long Term ◽  
Human And Mouse ◽  
Mouse Gut Microbiota

AbstractMany studies have revealed that both host and environmental factors can impact the gut microbial compositions, implying that the gut microbiota is considerably dynamic1–5. In their Article, Ji et al.6 performed comprehensive analysis of multiple high-resolution time series data of human and mouse gut microbiota. They found that both human and mouse gut microbiota dynamics can be characterized by several robust scaling laws describing short- and long-term changes in gut microbiota abundances, distributions of species residence and return times, and the correlation between the mean and the temporal variance of species abundances. They claimed that those scaling laws characterize both short- and long-term dynamics of gut microbiota. However, we are concerned that their interpretation is quite misleading, because all the scaling laws can be reproduced by the shuffled time series with completely randomized time stamps of the microbiome samples.

scholarly journals MetaPro-IQ: a universal metaproteomic approach to studying human and mouse gut microbiota

Microbiome ◽  
2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Xu Zhang ◽  
Zhibin Ning ◽  
Janice Mayne ◽  
Jasmine I. Moore ◽  
Jennifer Li ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Human And Mouse ◽  
Mouse Gut Microbiota

scholarly journals Cultured microbes represent a substantial fraction of the human and mouse gut microbiota

Gut Microbes ◽  
2017 ◽  
Vol 8 (5) ◽  
pp. 493-503 ◽  
Author(s):  
Ilias Lagkouvardos ◽  
Jörg Overmann ◽  
Thomas Clavel
Keyword(s):  
Gut Microbiota ◽  
Substantial Fraction ◽  
Human And Mouse ◽  
Mouse Gut Microbiota

scholarly journals Probiotic consumption influences universal adaptive mutations in indigenous human and mouse gut microbiota

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Chenchen Ma ◽  
Chengcheng Zhang ◽  
Denghui Chen ◽  
Shuaiming Jiang ◽  
Siyuan Shen ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Probiotic Strain ◽  
Single Nucleotide Variants ◽  
Efficacy Evaluation ◽  
Single Nucleotide ◽  
Intestinal Microbes ◽  
Adaptive Mutations ◽  
Probiotic Strains ◽  
Human And Mouse ◽  
Mouse Gut Microbiota

AbstractThe adaptive evolution in indigenous intestinal microbes derived from probiotics is critical to safety and efficacy evaluation of probiotics, yet it is still largely underexplored. Here, through 11 publicly accessible datasets, we demonstrated that probiotic consumption can lead to widespread single-nucleotide variants (SNVs) in the native microbiota. Interestingly, the same probiotic strains introduced far more SNVs in mouse gut than humans. Furthermore, the pattern of probiotics-induced SNVs was highly probiotic-strain specific, and 17 common SNVs in Faecalibacterium prausnitzii genome were identified cross studies, which might lead to changes in bacterial protein structure. Further, nearly 50% of F. prausnitzii SNVs can be inherited for six months in an independent human cohort, whereas the other half only transiently occurred. Collectively, our study substantially extended our understanding of co-evolution of the probiotics and the indigenous gut microbiota, highlighting the importance of assessment of probiotics efficacy and safety in an integrated manner.

scholarly journals Modulation of Enterohaemorrhagic Escherichia coli Survival and Virulence in the Human Gastrointestinal Tract

2018 ◽  
Vol 6 (4) ◽  
pp. 115 ◽  
Author(s):  
Grégory Jubelin ◽  
Mickaël Desvaux ◽  
Stephanie Schüller ◽  
Lucie Etienne-Mesmin ◽  
Maite Muniesa ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gastrointestinal Tract ◽  
Gut Microbiota ◽  
Model Systems ◽  
In Vivo Studies ◽  
Low Oxygen ◽  
Human Gastrointestinal Tract ◽  
Human Gut ◽  
Enterohaemorrhagic Escherichia Coli

Enterohaemorrhagic Escherichia coli (EHEC) is a major foodborne pathogen responsible for human diseases ranging from diarrhoea to life-threatening complications. Survival of the pathogen and modulation of virulence gene expression along the human gastrointestinal tract (GIT) are key features in bacterial pathogenesis, but remain poorly described, due to a paucity of relevant model systems. This review will provide an overview of the in vitro and in vivo studies investigating the effect of abiotic (e.g., gastric acid, bile, low oxygen concentration or fluid shear) and biotic (e.g., gut microbiota, short chain fatty acids or host hormones) parameters of the human gut on EHEC survival and/or virulence (especially in relation with motility, adhesion and toxin production). Despite their relevance, these studies display important limitations considering the complexity of the human digestive environment. These include the evaluation of only one single digestive parameter at a time, lack of dynamic flux and compartmentalization, and the absence of a complex human gut microbiota. In a last part of the review, we will discuss how dynamic multi-compartmental in vitro models of the human gut represent a novel platform for elucidating spatial and temporal modulation of EHEC survival and virulence along the GIT, and provide new insights into EHEC pathogenesis.

scholarly journals Comprehensive mouse gut metagenome catalog reveals major difference to the human counterpart

2021 ◽  
Author(s):  
Silas Kieser ◽  
Evgeny M. Zdobnov ◽  
Mirko Trajkovski
Keyword(s):  
Gut Microbiota ◽  
Gut Microbiome ◽  
New Order ◽  
Human Gut ◽  
Rarefaction Analysis ◽  
Human Counterpart ◽  
The Impact ◽  
Human And Mouse ◽  
Gut Metagenome ◽  
Mouse Gut Microbiota

AbstractMouse is the most used model for studying the impact of microbiota on its host, but the repertoire of species from the mouse gut microbiome remains largely unknown. Here, we construct a Comprehensive Mouse Gut Metagenome (CMGM) catalog by assembling all currently available mouse gut metagenomes and combining them with published reference and metagenome-assembled genomes. The 50’011 genomes cluster into 1’699 species, of which 78.1% are uncultured, and we discovered 226 new genera, 7 new families, and 1 new order. Rarefaction analysis indicates comprehensive sampling of the species from the mouse gut. CMGM enables an unprecedented coverage of the mouse gut microbiome exceeding 90%. Comparing CMGM to the human gut microbiota shows an overlap 64% at the genus, but only 16% at the species level, demonstrating that human and mouse gut microbiota are largely distinct.

scholarly journals Escherichia coli Exopolysaccharides Induced by Ceftriaxone Regulated Human Gut Microbiota in vitro

2021 ◽  
Vol 12 ◽  
Author(s):  
Baiyuan Li ◽  
Huahai Chen ◽  
Linyan Cao ◽  
Yunfei Hu ◽  
Dan Chen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gut Microbiota ◽  
Human Health ◽  
Molar Ratio ◽  
Composition Analysis ◽  
Rrna Gene ◽  
Sequencing Analysis ◽  
Human Gut ◽  
Human Gut Microbiota

A stable intestinal microflora is an essential prerequisite for human health. This study investigated the interaction between Escherichia coli exopolysaccharides (named EPS-m2) and the human gut microbiota (HGM) in vitro. The EPS-m2 was produced by E. coli WM3064 when treated with ceftriaxone. The monosaccharide composition analysis revealed that EPS-m2 is composed of glucuronic acid, glucose, fucose, galactose/N-acetyl glucosamine, arabinose, xylose, and ribose with a molar ratio of approximately 77:44:29:28:2:1:1. The carbohydrates, protein, and uronic acids contents in EPS-m2 was 78.6 ± 0.1%, 4.38 ± 0.11%, and 3.86 ± 0.09%, respectively. In vitro batch fermentation experiments showed that 77% of EPS-m2 could be degraded by human fecal microbiota after 72 h of fermentation. In reverse, 16S rRNA gene sequencing analysis showed that EPS-m2 increased the abundance of Alistipes, Acinetobacter, Alloprevotella, Howardella, and Oxalobacter; GC detection illustrated that EPS-m2 enhanced the production of SCFAs. These findings indicated that EPS-m2 supplementation could regulate the HGM and might facilitate modulation of human health.

scholarly journals Salicylate blockade of granulocyte adherence and the inflammatory response to experimental peritonitis

Blood ◽  
1979 ◽  
Vol 53 (5) ◽  
pp. 1018-1022 ◽  
Author(s):  
PJ Spagnuolo ◽  
JJ Ellner
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Streptococcus Pneumoniae ◽  
Inflammatory Response ◽  
Sodium Salicylate ◽  
Nylon Fiber ◽  
Intraperitoneal Infection ◽  
And Staphylococcus Aureus ◽  
Human And Mouse

Abstract Aspirin profoundly inhibited the in vitro augmentation of human and mouse granulocyte adherence to nylon fiber induced by the bacterial products Escherichia coli endotoxin and Staphylococcus aureus culture filtrate. Granulocytes obtained from normal volunteers during the 48 hr following ingestion of aspirin did not respond normally to endotoxin stimulation. Furthermore, pretreatment of mice with sodium salicylate prior to intraperitoneal infection with Streptococcus pneumoniae impaired granulocyte exudation and resulted in uncontrolled bacteremia and greater lethality of infection.

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