Insight into the protein salting-in mechanism of arginine, magnesium chloride and ethylene glycol: Solvent interaction with aromatic solutes

2021 ◽  
Author(s):  
Tomoto Ura ◽  
Tsutomu Arakawa ◽  
Kentaro Shiraki
Keyword(s):  
Ethylene Glycol ◽  
Magnesium Chloride ◽  
Solvent Interaction ◽  
Insight Into

Mapping of Allosteric Modulator Effects in the Active Site of Factor IXa.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 2695-2695
Author(s):  
Karla Villegas ◽  
Kimberly Baker-Deadmond ◽  
Pierre F. Neuenschwander
Keyword(s):  
Ethylene Glycol ◽  
Active Site ◽  
Allosteric Modulation ◽  
Factor Ix ◽  
Ionic Calcium ◽  
Gla Domain ◽  
A Site ◽  
Hydrolysis Of ◽  
Insight Into ◽  
Active Site Region

Abstract Activated factor IX (fIXa) is a vitamin K-dependent blood coagulation serine protease involved in propagation of the coagulant response through activation of fX. Maximal enzymatic and procoagulant activity of fIXa requires the presence of several cofactors; one of which is ionic calcium, which is known to bind to a site in the protease domain of fIXa as well as several sites within the light chain Gla domain region. One of the roles of calcium appears to be allosteric modulation of the fIXa active site as evidenced by an increase in enzymatic activity towards small peptidyl substrates. We and others have additionally found that certain small hygroscopic molecules can also enhance fIXa amidolytic activity. The molecular details involved in either of these effects are not well understood. Previous studies by us have shown that a pentapeptide substrate (AGRSL; the reactive site sequence of antithrombin) is hydrolyzed by fIXa in the absence of cofactors or modulators. This hydrolysis is enhanced in the presence of ionic calcium, ethylene glycol or low molecular weight heparin suggesting effects of these molecules on the immediate active site vicinity of fIXa. In order to gain insight into the potential allosteric modulation that each of these effectors may affect in fIXa, we examined the hydrolysis of four peptide libraries based on the AGRSL pentapeptide sequence, in the presence and absence of various fIXa modulators. The four libraries synthesized were XGRSL, AXRSL, AGRXL and AGRSX; where X denotes any of the possible 20 amino acids. Each of these libraries were screened for hydrolysis by fIXa under various conditions with substrates and products being identified en masse using MALDI-TOF mass spectrometry. The results suggest that ionic calcium enhances fIXa reactivity in part by modulation of the S2 subsite in fIXa. In contrast, ethylene glycol enhances fIXa activity via modulation of the S3 subsite and heparin was found to effect the overall active site region.

scholarly journals Metal extraction from a deep eutectic solvent, an insight into activities

2020 ◽  
Vol 22 (19) ◽  
pp. 11012-11024 ◽  
Author(s):  
Peng Cen ◽  
Kastriot Spahiu ◽  
Mikhail S. Tyumentsev ◽  
Mark R. St. J. Foreman
Keyword(s):  
Solvent Extraction ◽  
Ethylene Glycol ◽  
Metal Complexes ◽  
Deep Eutectic Solvent ◽  
Metal Extraction ◽  
Insight Into

By using the SIT equation and solvent extraction to consider activities of chloride and metal complexes in the aqueous and ethylene glycol layers, it is figuratively like viewing the system through a special lens which makes everything clearer.

New insight into electrosynthesis of ordered TiO2 nanotubes in EG-based electrolyte solutions: combined experimental and computational assessment

2020 ◽  
Vol 22 (39) ◽  
pp. 22719-22727
Author(s):  
Maryam Heydari Dokoohaki ◽  
Fatemeh Mohammadpour ◽  
Amin Reza Zolghadr
Keyword(s):  
Ethylene Glycol ◽  
Tio2 Nanotubes ◽  
Electrolyte Solutions ◽  
Electrochemical Anodization ◽  
Simulation Techniques ◽  
Insight Into

To obtain a better understanding of TiO2 nanotube (TiO2-NT) synthesis in different ethylene glycol (EG)-based electrolyte solutions by electrochemical anodization, the primary steps of TiO2-NT formation were studied by experimental and simulation techniques.

An insight into the novel ring transformation reactions using ethylene glycol/BF3

Tetrahedron ◽  
1991 ◽  
Vol 47 (40) ◽  
pp. 8523-8528 ◽  
Author(s):  
Takayoshi Yamamoto ◽  
Hiroshi Suemune ◽  
Kiyoshi Sakai
Keyword(s):  
Ethylene Glycol ◽  
The Novel ◽  
Ring Transformation ◽  
Insight Into

STUDY OF THERMODYNAMICAL PROPERTIES OF POLYVINYL ACETATE IN TETRAHYDROFURAN

2012 ◽  
Vol 26 (27) ◽  
pp. 1250180 ◽  
Author(s):  
PRIYANKA V. TABHANE ◽  
OMPRAKASH P. CHIMANKAR ◽  
VILAS A. TABHANE
Keyword(s):  
Ultrasonic Velocity ◽  
Polyvinyl Acetate ◽  
Adiabatic Compressibility ◽  
Ultrasonic Waves ◽  
Acoustical Parameters ◽  
Solvent Interaction ◽  
Pulse Echo ◽  
The Given ◽  
Insight Into ◽  
Made In

The propagation of ultrasonic waves and the measurement of their velocity in solutions form an important tool for the evaluation of various acoustical and thermodynamical parameters which give an insight into the nature of miscibility and molecular interactions in polymer solutions. In the present study, the ultrasonic velocity, density, viscosity measurements have been made in 0.1 M solution using pulse echo overlap technique at 293 K at the different concentrations of polyvinyl acetate in tetrahydrofuran. Thermo-acoustical parameters viz., adiabatic compressibility, molar sound velocity, molar compressibility, expansion coefficient, acoustic impedance, van der Waals' constant and internal pressure have been computed from the experimental data. The nature of polymer/solvent interaction and the effect of concentration on the molecular interaction are studied. The nonlinear variations of polyvinyl acetate in tetrahydrofuran have been studied. The variation of ultrasonic velocity and other thermo-acoustical parameters shows nonlinear increase or decrease with molar concentration which suggest semi-compatibility of the polymer in the given solvent.

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