Antibacterial effects of Chitosan solution® against Legionella pneumophila, Escherichia coli, and Staphylococcus aureus

2006 ◽  
Vol 112 (2) ◽  
pp. 96-101 ◽  
Author(s):  
Takanori Fujimoto ◽  
Yasuo Tsuchiya ◽  
Michinori Terao ◽  
Kazutoshi Nakamura ◽  
Masaharu Yamamoto
Author(s):  
Odette N. Kabena ◽  
Naomie E. Nyakembe ◽  
Jean Jacques D. Amogu ◽  
Lionel S. Asamboa ◽  
Emmanuel M. Lengbiye ◽  
...  

Aim: The aim of this work is to evaluate the spermicidal, antimicrobial activity of Oncoba welwitchii Oliv. This will justify its use on reproductive health especially in the occurrence of infertility and the imbalance of vaginal flora. Carried out at the Department of Biology of the Faculty of Sciences of the University of Kinshasa, between January and May 2019, this study was initiated to determine the effect of the use of O. welwitchii Oliv. on human spermatozoa and vaginal microorganisms. Methodology: The leaves of O. welwitchii Oliv. were collected and identified at University of Kinshasa. The spermicidal effect was performed by the Sander-Cramer method and antibacterial activity were evaluated by liquid dilution method. Results: The results revealed that the juice of the leaves of O. welwitchii Oliv. have a spermicidal effect, as this juice reduce the initial mobility of the spermatozoids after 60 minutes of the exposure from 72±12% to 1±2% and their initial viability from 74±11% to 10±18%. This juice has shown bacteriostatic effects against Escherichia coli and Staphylococcus aureus (MBC/MIC > 4) and bactericidal effects against Lactobacilus acidophilus (MBC/MIC ≤ 4). Conclusion: This study gives additional information on the phytochemistry composition and a spermicidal effects of O. welwitchii Oliv. The spermicidal effects of this plant could be associated to its acidity. It should be noted that to the best of our knowledge, no study on the phytochemistry of O. welwitchii Oliv. is available in the literature.


2005 ◽  
Vol 10 (5) ◽  
pp. 233-238 ◽  
Author(s):  
Yasuo TSUCHIYA ◽  
Michihiro TERAO ◽  
Takanori FUJIMOTO ◽  
Kazutoshi NAKAMURA ◽  
Masaharu YAMAMOTO

2012 ◽  
Vol 1 (2) ◽  
Author(s):  
Yugo Berri Putra Rio ◽  
Aziz Djamal ◽  
Asterina Asterina

AbstrakPendahuluan: Madu digunakan sebagai agen makanan dan obat tradisional, mengandung nektar atau gulaeksudat dari tanaman yang dikumpulkan oleh lebah madu serta merupakan salah satu obat tradisional yangdigunakan oleh masyarakat. Madu diketahui memiliki kemampuan sebagai efek antibakteri, seperti Escherichia colidan Stahpylococcus aureus. Kedua bakteri ini memiliki sifat yang berbeda tetapi memilki kesamaan dari penyakityang disebabkannya. Kualitas dan jenis madu juga sesuai dengan tempat dan lokasi lebah berkembang biak.Tujuan penelitian ini adalah untuk melihat perbandingan efek antibakteri madu asli Sikabu dan Lubuk Minturunterhadap Escherichia coli dan Staphylococcus aureus secara in vitro. Metode Penelitian: Madu yang diuji untukpenelitian ini adalah madu yang berasal dari Sikabu dan Lubuk Minturun. Penelitian ini dilaksanakan pada bulanDesember 2011 sampai Mei 2012 di laboratorium Mikrobiologi Fakultas Kedokteran Universitas Andalas. Jenispenelitian yang digunakan adalah eksperimental dengan menggunakan metode difusi (metode cakram) dananalitik dengan menghubungkan perbedaan yang dimiliki kedua madu sebagai efek antibakteri. Hasil Penelitian:ini menunjukkan bahwa madu asli Sikabu dan Lubuk Minturun tidak memiliki kemampuan efek antibakteri terhadapEscherichia coli, dan terdapat perbedaan efek antibakteri dari kedua madu terhadap Staphylococcus aureus.Kesimpulan: dari kedua jenis madu yang diteliti tidak ditemukan efek antibakteri terhadap Escherichia coli , namunmadu asli Sikabu memiliki efek antibakteri yang lebih baik dari pada madu Lubuk Minturun terhadapStaphylococcus aureusKata Kunci : Perbandingan Efek Antibakteri, Madu Asli Sikabu, Madu Lubuk Minturun. Escherichia coli,Staphylococcus aureusAbstractIntroduction: Honeyis used as an agent for food and traditional medicine, containing nectar or sugar exudates ofthe plant collected by honey bees, is one of the traditional medicines used by community. Honey is known to havethe ability as an antibacterial effect, such as Escherichia coli and Stahpylococcus aureus. Both of these bacteriahave different properties but have the common of the diseases it causes. The quality and type of honey is also inaccordance with the breeding places and their location. The purpose of this study was to see a comparison of theantibacterial effect of honey Sikabu and Lubuk Minturun against Escherichia coli and Staphylococcus aureus by invitro. Methods: Honey to be tested for this study were derived from Sikabu and Lubuk Minturun. This study wasconducted in December 2011 to May 2012 in the laboratory of Microbiology, Faculty of Medicine, AndalasUniversity. Type of research is experimental diffusion method (disc method) and analytical distinction owned bylinking the two of honey as an antibacterial effect. Results: These results indicate that the honey Sikabu and LubukMinturun have no antibacterial effect against Escherichia coli, both of honey have differences antibacterial effectagainst Staphylococcus aureus. Conclusion: Both types of honey from this study did not find an antibacterial effectagainst Escherichia coli, but honey Sikabu has better antibacterial against Staphylococcus aureus than honeyLubuk Minturun.Keywords: Comparisons Antibacterial Effects, Original Sikabu Honey, Honey Lubuk Minturun. Escherichia coli,Staphylococcus aureus


2018 ◽  
Vol 69 (6) ◽  
pp. 1485-1488
Author(s):  
Marioara Nicoleta Filimon ◽  
Roxana Popescu ◽  
Adrian Sinitean ◽  
Paula Maniu ◽  
Gabi Dumitrescu ◽  
...  

The interest in the antimicrobial actions of chitosan is due to its multiple properties and effects. The aim of the study was to assess the potential antibacterial effects of chitosan applied on 7 bacterial strains: Escherichia coli, Streptococcus pyogenes, Pseudomonas aeruginosa, Enterococcus faecalis, Clostridium perfringens, Legionella pneumophila and Staphylococcus aureus. Six different concentrations of chitosan were dissolved in 1% acetic acid, following two working protocols (Kirby-Bauer method and testing for bacterial cell viability). The sensitivity of tested bacterial strains following the effect of exposure to chitosan decreased as follows: E. coli] L. pneumophila] S. aureus] S. pyogenes] C. perfringens] P. aeruginosa] E. faecalis. The inhibition rates for the bacterial strains E. faecalis, S. pyogenes and S. aureus highlighted again the strong antibacterial properties of this product. Conclude that the chitosan presents a different antibacterial effect against several bacterial strains of interest directly with the employed concentrations.


2020 ◽  
Vol 10 (3) ◽  
pp. 316-329
Author(s):  
Fateme Mirzajani ◽  
Amin Hamidi

Introduction: In this project, the growth and volatile metabolites profiles of Escherichia coli (E. coli ) and Staphylococcus aureus were monitored under the influence of silver base chemical, nanoparticle and ultra-highly diluted compounds. Materials & Methods: The treatments were done for 12000 life cycles using silver nanoparticles (AgNPs) as well as ultra-highly diluted Argentum nitricum (Arg-n). Volatile organic metabolites analysis was performed using gas chromatography mass spectrometry (GC-MS). The results indicated that AgNPs treatment made the bacteria resistant and adapted to growth in the nanoparticle condition. The use of ultra-highly diluted Arg-n initially increased growth but it decreased later. Also, with the continuous usage of these materials, no more bacterial growth was observed. Results: The most important compounds produced by E. coli are Acetophenone, Octyl acetate, Styrene, 1,8-cineole, 4-t-butyl-2-(1-methyl-2-nitroethyl)cyclohexane, hexadecane and 2-Undecanol. The main compounds derived from S. aureus are Acetophenone,1,8-cineole, Benzaldehyde, 2-Hexan-1-ol, Tridecanol, Dimethyl Octenal and tetradecane. Acetophenone and 1,8-cineole were common and produced by both organisms. Conclusion: Based on the origin of the produced volatiles, main volatiles percentage of untreated sample is hydrocarbon (>50%), while bacteria treatments convert the ratio in to aldehydes, ketones and alcohols in the case of AgNPs, (>80%) and aldehydes, ketones and terpenes in the case of Arg-n (>70%).


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