In Vitro Antibacterial Effects of Chloroform, Methanol and Water Extracts of Croton Macrostachyus Stem Bark Against Escherichia Coli and Staphylococcus Aureus Standard and Clinical Strains

AbstrakPendahuluan: Madu digunakan sebagai agen makanan dan obat tradisional, mengandung nektar atau gulaeksudat dari tanaman yang dikumpulkan oleh lebah madu serta merupakan salah satu obat tradisional yangdigunakan oleh masyarakat. Madu diketahui memiliki kemampuan sebagai efek antibakteri, seperti Escherichia colidan Stahpylococcus aureus. Kedua bakteri ini memiliki sifat yang berbeda tetapi memilki kesamaan dari penyakityang disebabkannya. Kualitas dan jenis madu juga sesuai dengan tempat dan lokasi lebah berkembang biak.Tujuan penelitian ini adalah untuk melihat perbandingan efek antibakteri madu asli Sikabu dan Lubuk Minturunterhadap Escherichia coli dan Staphylococcus aureus secara in vitro. Metode Penelitian: Madu yang diuji untukpenelitian ini adalah madu yang berasal dari Sikabu dan Lubuk Minturun. Penelitian ini dilaksanakan pada bulanDesember 2011 sampai Mei 2012 di laboratorium Mikrobiologi Fakultas Kedokteran Universitas Andalas. Jenispenelitian yang digunakan adalah eksperimental dengan menggunakan metode difusi (metode cakram) dananalitik dengan menghubungkan perbedaan yang dimiliki kedua madu sebagai efek antibakteri. Hasil Penelitian:ini menunjukkan bahwa madu asli Sikabu dan Lubuk Minturun tidak memiliki kemampuan efek antibakteri terhadapEscherichia coli, dan terdapat perbedaan efek antibakteri dari kedua madu terhadap Staphylococcus aureus.Kesimpulan: dari kedua jenis madu yang diteliti tidak ditemukan efek antibakteri terhadap Escherichia coli , namunmadu asli Sikabu memiliki efek antibakteri yang lebih baik dari pada madu Lubuk Minturun terhadapStaphylococcus aureusKata Kunci : Perbandingan Efek Antibakteri, Madu Asli Sikabu, Madu Lubuk Minturun. Escherichia coli,Staphylococcus aureusAbstractIntroduction: Honeyis used as an agent for food and traditional medicine, containing nectar or sugar exudates ofthe plant collected by honey bees, is one of the traditional medicines used by community. Honey is known to havethe ability as an antibacterial effect, such as Escherichia coli and Stahpylococcus aureus. Both of these bacteriahave different properties but have the common of the diseases it causes. The quality and type of honey is also inaccordance with the breeding places and their location. The purpose of this study was to see a comparison of theantibacterial effect of honey Sikabu and Lubuk Minturun against Escherichia coli and Staphylococcus aureus by invitro. Methods: Honey to be tested for this study were derived from Sikabu and Lubuk Minturun. This study wasconducted in December 2011 to May 2012 in the laboratory of Microbiology, Faculty of Medicine, AndalasUniversity. Type of research is experimental diffusion method (disc method) and analytical distinction owned bylinking the two of honey as an antibacterial effect. Results: These results indicate that the honey Sikabu and LubukMinturun have no antibacterial effect against Escherichia coli, both of honey have differences antibacterial effectagainst Staphylococcus aureus. Conclusion: Both types of honey from this study did not find an antibacterial effectagainst Escherichia coli, but honey Sikabu has better antibacterial against Staphylococcus aureus than honeyLubuk Minturun.Keywords: Comparisons Antibacterial Effects, Original Sikabu Honey, Honey Lubuk Minturun. Escherichia coli,Staphylococcus aureus

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Antibacterial activity of different extract of medicinal plant Antirrihnum Orontium.

Journal Of Tropical Pharmacy And Chemistry ◽

10.25026/jtpc.v5i1.221 ◽

2020 ◽

Vol 5 (1) ◽

Author(s):

Himayat Ullah ◽

Shehzad Saleem ◽

Wisal Ahmad ◽

Muhammad Hanif ◽

Zahid Ali ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Water Extract ◽

Flowering Plant ◽

Diffusion Method ◽

Water Extracts ◽

The Family ◽

Quaternary Base ◽

And Staphylococcus Aureus

Antirrhinum Orontium is belonging to the family Plantaginacea commonly known as lesser snapdragon which is a flowering plant belong to genus Antirrhinum. It was traditionally used in treatment of Tumor, Eye inflammation and Ulcer. Antirrhinum Orontium is contain mostly tertiary alkaloids bases one of them is identified as 4-methyl-2, 6-naphthyridine and Choline is identified as quaternary base. The recent study is to investigate the antibacterial activity of Ethyl acetate, Dichloromethane, Hexane and Water extracts of Antirrhinum Orontium, against the pathogen like Escherichia coli and Staphylococcus aureus. Both the Soxhlet and Maceration water extract were found most active from the other extract against Escherichia coli and Staphylococcus aureus by using agar well diffusion method. The measured zone of inhibition for Soxhlet water extract is 21±0.05mm and Maceration water extract is 20±0.05mm against gram positive Staphylococcus aureus, while for gram negative Escherichia coli 23±0.05mm is measured for Soxlet and 21±0.05mm measured for Maceration water extracts

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Salicylate blockade of granulocyte adherence and the inflammatory response to experimental peritonitis

Blood ◽

10.1182/blood.v53.5.1018.bloodjournal5351018 ◽

1979 ◽

Vol 53 (5) ◽

pp. 1018-1022

Author(s):

PJ Spagnuolo ◽

JJ Ellner

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Streptococcus Pneumoniae ◽

Inflammatory Response ◽

Sodium Salicylate ◽

Nylon Fiber ◽

Intraperitoneal Infection ◽

And Staphylococcus Aureus ◽

Human And Mouse

Aspirin profoundly inhibited the in vitro augmentation of human and mouse granulocyte adherence to nylon fiber induced by the bacterial products Escherichia coli endotoxin and Staphylococcus aureus culture filtrate. Granulocytes obtained from normal volunteers during the 48 hr following ingestion of aspirin did not respond normally to endotoxin stimulation. Furthermore, pretreatment of mice with sodium salicylate prior to intraperitoneal infection with Streptococcus pneumoniae impaired granulocyte exudation and resulted in uncontrolled bacteremia and greater lethality of infection.

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In-vitro antibacterial effects of methanolic extract of peppermint (Mentha Piperita Lamiaceae) on standard Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa strain

Jorjani Biomedicine Journal ◽

10.29252/jorjanibiomedj.7.4.4 ◽

2019 ◽

Vol 7 (4) ◽

pp. 4-10

Author(s):

Samira Mahmoudi ◽

Rozita Nasiri ◽

Abolfazl Jafari Sales ◽

◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Bacillus Cereus ◽

Methanolic Extract ◽

Mentha Piperita ◽

Antibacterial Effects

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Evaluation preliminary phytochemistry and antimicrobial activity of extracts in Eugenia uniflora L. on Escherichia coli and Staphylococcus aureus

Research Society and Development ◽

10.33448/rsd-v10i13.20579 ◽

2021 ◽

Vol 10 (13) ◽

pp. e329101320579

Author(s):

Alexandre Horácio Couto Bittencourt ◽

Jaqueline Souza Machado ◽

Mariana Garcia da Silva ◽

Braz Antônio Pereira Cosenza

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Eugenia Uniflora ◽

E Coli ◽

And Staphylococcus Aureus

Devido ao aumento da resistência de patógenos humanos e animais aos medicamentos atuais, pesquisas por novos antimicrobianos têm sido incentivadas, principalmente por meio de plantas medicinais. Eugenia uniflora L., conhecida como pitangueira, é popularmente utilizada com diversas funções terapêuticas, justificando estudos nesta espécie. Assim, os objetivos deste estudo foram avaliar a atividade antibacteriana “in vitro” de extratos de folhas e cascas de Eugenia uniflora L. sobre Escherichia coli e Staphylococcus aureus. Para as etapas de triagem fitoquímica e microbiologia, foram utilizadas as metodologias usuais nessas áreas para obtenção dos extratos e avaliação da atividade antibacteriana com uso de controle positivo e negativo. Os resultados obtidos mostraram que no extrato das folhas foi verificada a presença de saponinas, flavonóides, alcalóides e taninos, enquanto na casca foram obtidos apenas saponinas e alcalóides. Os extratos da folha e da casca foram capazes de inibir o crescimento de Stapylococcus aureus apenas, promovendo halos de inibição de meio de 19,4 mm e 14,4 mm, respectivamente. As análises permitiram concluir que os extratos apresentam metabólitos secundários interessantes e de grande potencial antimicrobiano e que esses extratos foram capazes de inibir apenas as cepas de S. aureus, sendo resistentes às cepas de E. Coli.

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In vitro bactericidal activity of diterpenoids isolated from Aframomum melegueta K. Schum against strains of Escherichia coli, Listeria monocytogenes and Staphylococcus aureus

Planta Medica ◽

10.1055/s-0034-1394918 ◽

2014 ◽

Vol 80 (16) ◽

Author(s):

K Ngwoke ◽

O Chevallier ◽

V Wirkom ◽

P Stevenson ◽

C Elliott ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Listeria Monocytogenes ◽

Bactericidal Activity ◽

And Staphylococcus Aureus

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Anti-Bacterial from Moringa oleifera Lamk Crude Extracts

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.1060.223 ◽

2014 ◽

Vol 1060 ◽

pp. 223-226

Author(s):

Perayot Pamonsinlapatham

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Moringa Oleifera ◽

Soxhlet Extraction ◽

Stem Bark ◽

Bacterial Activity ◽

Diffusion Method ◽

Crude Extracts ◽

Agar Diffusion Method

Moringa oleiferaLamk (Moringaceae) is a worldwide use for food and traditional medicine. In this study, plants were separated into seven parts: 1) Leaf, 2) Drum, 3) Branching, 4) Seed, 5) Outer stem, 6) Stem bark, and 7) Root, respectively. Then, they were extracted by two different methods: methanol soxhlet extraction and ethanol maceration. All crude extracts were tested for anti-bacterial activity by agar diffusion method and colorimetric micro-dilution plate technique. The results showed that 7 parts of Moringa crude extracts had low anti-bacterial activity againstStaphylococcus aureus,Escherichia coli, andPseudomonas aeruginosawhen compared with standard antibiotic (ampicillin or gentamycin). In conclusion, anti-bacterial effects ofM. oleiferacrude extracts had low anti-bacterial activityin vitrotest.

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Compatibility of Cephalosporins and Aminogl Ycosides in Peritoneal Dialysis Fluid

Peritoneal Dialysis International ◽

10.1177/089686088300300305 ◽

1983 ◽

Vol 3 (3) ◽

pp. 128-129 ◽

Cited By ~ 7

Author(s):

Carol Loeppky ◽

Eugene Tarka ◽

E. Dale Everett

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Peritoneal Dialysis ◽

In Vitro Studies ◽

Dialysis Fluid ◽

Peritoneal Dialysis Fluid ◽

The Individual ◽

And Staphylococcus Aureus

Often dialysis -associated peritonitis is treated before the results of cultures are known with a cephalosporin and an aminoglycoside in combination. Because there may be antagonism between the individual drugs in such combinations, we have investigated this possibility through the use of timed, killing curves in dialysate effluent. We tested various cephalosporins and aminoglycosides alone and in combination at concentrations usually instilled into the peritoneum and determined their activity against one strain each of Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. The results of these in-vitro studies show no evidence of antagonism but rather suggest an additive effect as evidenced by more rapid killing.

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Antibacterial Properties of Nanosilver PLLA Fibrous Membranes

Journal of Nanomaterials ◽

10.1155/2009/168041 ◽

2009 ◽

Vol 2009 ◽

pp. 1-5 ◽

Cited By ~ 9

Author(s):

Lin Li ◽

Yi Li ◽

Jiashen Li ◽

Lei Yao ◽

Arthur F. T. Mak ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Silver Nanoparticles ◽

Antibacterial Properties ◽

Weight Ratio ◽

Antibacterial Effects ◽

E Coli ◽

Fibrous Membranes ◽

Composite Fibrous Membranes

Nanosilver has been studied as a valuable material for it strong antibacterial effects. In this study, we investigated the antibacterial properties of nano silver Poly-L-Lactic acid (Ag/PLLA) composite fibrous membranes. Ag/PLLA fibrous membranes were prepared with silver nanoparticles having weight ratio of silver nanoparticles to PLLA at 5% (w/w). In vitro antibacterial tests were performed usingEscherichia coli(E. coli) andStaphylococcus aureus(Staph.) to determine the antibacterial capability of the Ag/PLLA fibrous membranes. As the results suggested, Ag/PLLA fibrous membranes showed strong antibacterial properties. Thus, Ag/PLLA fibrous membrane can be used as an antibacterial scaffold for tissue engineering.

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