Characterization of interactions between Escherichia coli O157:H7 with epiphytic bacteria in vitro and on spinach leaf surfaces

2012 ◽  
Vol 153 (3) ◽  
pp. 351-357 ◽  
Author(s):  
Gabriela Lopez-Velasco ◽  
Heather A. Tydings ◽  
Renee R. Boyer ◽  
Joseph O. Falkinham ◽  
Monica A. Ponder
Keyword(s):  
Escherichia Coli ◽  
Escherichia Coli O157 ◽  
Epiphytic Bacteria ◽  
Leaf Surfaces ◽  
Spinach Leaf

scholarly journals Inhibition of Escherichia coli O157:H7 and Salmonella enterica Isolates on Spinach Leaf Surfaces Using Eugenol-Loaded Surfactant Micelles

Foods ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 575
Author(s):  
Songsirin Ruengvisesh ◽  
Chris R. Kerth ◽  
T. Matthew Taylor
Keyword(s):  
Escherichia Coli ◽  
Salmonella Enterica ◽  
Limit Of Detection ◽  
Sodium Dodecyl ◽  
Microbial Pathogens ◽  
Escherichia Coli O157 ◽  
Human Pathogens ◽  
Surfactant Micelles ◽  
Leaf Surfaces ◽  
Spinach Leaf

Spinach and other leafy green vegetables have been linked to foodborne disease outbreaks of Escherichia coli O157:H7 and Salmonella enterica around the globe. In this study, the antimicrobial activities of surfactant micelles formed from the anionic surfactant sodium dodecyl sulfate (SDS), SDS micelle-loaded eugenol (1.0% eugenol), 1.0% free eugenol, 200 ppm free chlorine, and sterile water were tested against the human pathogens E. coli O157:H7 and Salmonella Saintpaul, and naturally occurring microorganisms, on spinach leaf surfaces during storage at 5 °C over 10 days. Spinach samples were immersed in antimicrobial treatment solution for 2.0 min at 25 °C, after which treatment solutions were drained off and samples were either subjected to analysis or prepared for refrigerated storage. Whereas empty SDS micelles produced moderate reductions in counts of both pathogens (2.1–3.2 log10 CFU/cm2), free and micelle-entrapped eugenol treatments reduced pathogens by >5.0 log10 CFU/cm2 to below the limit of detection (<0.5 log10 CFU/cm2). Micelle-loaded eugenol produced the greatest numerical reductions in naturally contaminating aerobic bacteria, Enterobacteriaceae, and fungi, though these reductions did not differ statistically from reductions achieved by un-encapsulated eugenol and 200 ppm chlorine. Micelles-loaded eugenol could be used as a novel antimicrobial technology to decontaminate fresh spinach from microbial pathogens.

Cloning and characterization of GDP-perosamine synthetase (Per) from Escherichia coli O157:H7 and synthesis of GDP-perosamine in vitro

2007 ◽  
Vol 363 (3) ◽  
pp. 525-530 ◽  
Author(s):  
Guohui Zhao ◽  
Jun Liu ◽  
Xiang Liu ◽  
Min Chen ◽  
Houcheng Zhang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Escherichia Coli O157

Infrared Sensor–Based Aerosol Sanitization System for Controlling Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes on Fresh Produce

2014 ◽  
Vol 77 (6) ◽  
pp. 977-980 ◽  
Author(s):  
SANG-OH KIM ◽  
JAE-WON HA ◽  
KI-HWAN PARK ◽  
MYUNG-SUB CHUNG ◽  
DONG-HYUN KANG
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Salmonella Typhimurium ◽  
Fresh Produce ◽  
Sensor Technology ◽  
Escherichia Coli O157 ◽  
Full Time ◽  
Infrared Sensor ◽  
Leaf Surfaces ◽  
Spinach Leaf

An economical aerosol sanitization system was developed based on sensor technology for minimizing sanitizer usage, while maintaining bactericidal efficacy. Aerosol intensity in a system chamber was controlled by a position-sensitive device and its infrared value range. The effectiveness of the infrared sensor–based aerosolization (ISA) system to inactivate Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes on spinach leaf surfaces was compared with conventional aerosolization (full-time aerosol treated), and the amount of sanitizer consumed was determined after operation. Three pathogens artificially inoculated onto spinach leaf surfaces were treated with aerosolized peracetic acid (400 ppm) for 15, 30, 45, and 60 min at room temperature (22 ± 2°C). Using the ISA system, inactivation levels of the three pathogens were equal or better than treatment with conventional full-time aerosolization. However, the amount of sanitizer consumed was reduced by ca. 40% using the ISA system. The results of this study suggest that an aerosol sanitization system combined with infrared sensor technology could be used for transportation and storage of fresh produce efficiently and economically as a practical commercial intervention.

scholarly journals Isolation and Characterization of a New T-Even Bacteriophage, CEV1, and Determination of Its Potential To Reduce Escherichia coli O157:H7 Levels in Sheep

2006 ◽  
Vol 72 (9) ◽  
pp. 6405-6410 ◽  
Author(s):  
Raul R. Raya ◽  
Peter Varey ◽  
Rebecca A. Oot ◽  
Michael R. Dyen ◽  
Todd R. Callaway ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Oral Dose ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Isolation And Characterization ◽  
Unit Reduction

ABSTRACT Bacteriophage CEV1 was isolated from sheep resistant to Escherichia coli O157:H7 colonization. In vitro, CEV1 efficiently infected E. coli O157:H7 grown both aerobically and anaerobically. In vivo, sheep receiving a single oral dose of CEV1 showed a 2-log-unit reduction in intestinal E. coli O157:H7 levels within 2 days compared to levels in the controls.

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